The Characteristics of Semi-Cokes --The Solid Residues from Coal Partial Gasification

In this paper the proximate analysis and ultimate analysis of sulfur in different semi-cokes generated from Rizhao bituminous coal and Beijing anthracite under different temperatures is done. Also the tendency of the contents of volatile, ash, fixed carbon and sulfur in different semi-cokes along with the different preparation temperatures is studied. Then the combustion experiment of semi-cokes in the drop-tube furnace system was carried out, and the kinetic parameters of different semi-cokes ware calculated.

Self-gelatinizable graft copolymer of poly(vinyl alcohol) with 4-vinylpyridine as an immobilization matrix for the construction of a tyrosinase-based amperometric biosensor

A tyrosinase-based amperometric biosensor using a self-gelatinizable graft copolymer of poly(vinyl alcohol) with 4-vinylpyridine (PVA-g-PVP) as an immobilization matrix was constructed. The 4-vinylpyridine component of PVA-g-PVP enhances the adherence to a glassy carbon electrode surface. The content of 4-vinylpyridine in this immobilization matrix plays a key role in retaining the activity of tyrosinase. A simple, milder method was adopted by simply syringing the copolymer-tyrosinase aqueous solution on to the electrode surface and allowing water to evaporate at 4 degrees C in a refrigerator. Several parameters, including copolymer composition; pH, applied potential and enzyme membrane composition, ware optimized. The enzyme membrane composition can be varied to obtain higher sensitivity or a wider linear detection range. The biosensor was used for the determination of phenol, p-cresol and catechol. The biosensor exhibited excellent reproducibility, stability and sensitive response and can be used in flow injection analysis. The biosensor showed an extended linear range in hydrophilic organic solvents and it can be used in monitoring organic reaction processes. The analytical performance demonstrated this immobilization matrix is suitable for the immobilization of tyrosinase.

玻璃海鞘多肽PCI的分离纯化及其抗血管生成活性研究

血管生成是肿瘤生长、发展的必经之路，并且与实体瘤的发生、转移有着密切的关系，抑制肿瘤新生血管生成具有特异性高、疗效好、不易产生耐药性以及毒副作用低等特点，因此抑制肿瘤血管形成可望成为治疗癌症的一个突破点，以抗血管生成为主的肿瘤生物治疗研究已成为近十年的研究热点。玻璃海鞘（Coina intestinalis）属于内性目、玻璃海鞘科，因其进化上的独特地位常作为研究神经发育、免疫系统进化的材料。在其它种属的海鞘中分离发现了多种具有抗肿瘤活性的多肽，但关于玻璃海鞘抗血管生成活性多肽的分离纯化和活性研究未见报道。本文利用多种分离纯化手段，采用活性追踪的方法首次从玻璃海鞘中分离得到具有抗新生血管生成作用的多肽，并对其抗血管生成活性做了初步研究。 本研究利用冷丙酮分级沉淀，超滤截留分子量小于5kDa的蛋白，再经SephadexG25、Superdex75柱层析，µRPC C2/C18反相柱层析等分离手段，采用活性追踪的方法，由玻璃海鞘（Coina intestinalis）中分离纯化出抗血管生成多肽PCI，据保留时间计算其分子量为1.8 kDa。MTT检测表明其对人脐静脉血管内皮细胞（HUVEC）具有强烈的抑制作用，IC50为7.5 μg/ml，并对多种肿瘤细胞有直接的抑制作用。斑马鱼胚胎体内实验进一步表明PCI在40 μg/ml的浓度下作用12h，斑马鱼胚胎新生血管生成受到显著抑制，肠下静脉血管长度为正常组的30%，斑马鱼胚胎血管生成率为正常组的45%。

CIM思想指导下的工业自动化总体技术

工业自动化总体技术是在ＣＩＭ思想指导下产生的实施ＣＩＭ的关键技术。总体技术的核心是总体设计与总体集成技术。本文言及总体技术产生的背景，其宗旨与含义，总体技术方法学，工业自动化系统体系结构与参考模型，以及实践总体技术的概况动向。

基于中间件的MES与ERP系统信息集成技术研究

现在企业系统与控制系统的主流的集成技术为基于中间件的系统集成,即通过中间件的桥梁作用把各种ERP和MES系统连接起来,实现信息在两者之间的交互与共享。因此本文提出“信息集成内容遵循S95标准,消息封装格式应用XML技术,企业应用集成平台采用BizTalkserver来实现”的中间件集成方法,最后应用此中间件引擎给出了一个在MES与ERP系统之间实现信息集成的示例。

满意度测量问卷之研制

Job satisfaction is the basic component of organizational performance. At present, however, there is no instrument to be used to measure this variable in chine. This research was about the measurement of job satisfaction. In our research, We use Minnesota Satisfaction Questionnaire (or MSQ) as a base, then put anther 20 items to form out original questionnaire. After two round measurements and carefully analysis, finally, We got a new questionnaire. In the questionnire; 6 of the 20 MSQ items were discarded, and anther 6 items put in. The form of our questionnire is similar to MSQ. But this research also shown that there ware some problems in it, and advarnce research should be taken to improve the research results.