第18届国际理论与应用力学大会(ICTAM-18)

<正> 由国际理论与应用力学联合会(IUTAM)召开的4年一次的第18届国际理论与应用力学大会(ICTAM-18),于1992年8月22—28日在以色列海法市的以色列工业学院举行。大会选定的3个主题是:固体与结构力学中的不稳定性;海面力学与海气相互作用;生物力学。设置主题分会的目的是对力学中的某些重要领域做综合介绍,以强调这领域的重要性,或对新领域起提倡作用。

激光毛化技术对18-8不锈钢疲劳性能影响的研究

通过模拟分析 ,对激光毛化 18 8不锈钢的疲劳寿命与毛化覆盖率的关系进行研究 ,预测结果与相关实验结果进行对比。结果显示 ,由于激光毛化处理的影响 ,18 8不锈钢的疲劳寿命得到改善 ,而且在毛化覆盖率为 2 0 %左右时 ,材料疲劳性能最好

脉冲激光辐照ZrO_2涂层的18-8不锈钢快凝组织与精细结构

利用脉冲辐照研究超高温度梯度下表层预轩ZrO_2纳米颗粒涂层的18-8奥氏体不锈钢的快凝组织形态、微结构特征及力学性质，结果表明，在表层约15μm范围内形成Zr的合金化微区，Zr最大含量(原子分数)为43.3%，但Zr成分分布存在不连续特征；预置纳米ZrO_2陶瓷涂层明显提高了激光作用深度。超高温梯度和高冷却速度显著细化了快凝亚组织并得到超细枝晶及胞晶，并观察到位错胞及栅栏孪晶两类亚晶，而激光熔区的外延生长机制未使晶粒细化。激光辐照后，合金化区硬度增加而弹性模量降低，其余部分硬度增加而弹性模量无变化。

相位锁定双包层光纤激光器阵列获得18．3W的相干输出

光纤激光器以其结构紧凑、转换效率高、易散热和光束质量好等优点正逐渐成为高功率全固态激光器的主要选择。目前，单根光纤激光器的输出功率已经超过千瓦量级，但是由于受受激拉曼散射（SRS）和受激布里渊散射（SBS）等非线性效应的影响，单根光纤激光器的最终输出功率还是受到限制。要在保证光束质量的同时进一步提高输出功率就需要采用相干组束技术。

云南18 个民族Y染色体双等位基因单倍型频率的主成分分析

世居云南的少数民族中,壮、傣、水、布依、布朗、德昂、佤、彝、白、怒、哈尼、傈僳、拉祜、纳西、景颇、阿昌、基 诺和独龙18 个民族是由“羌”、“濮”、“越”3 大部落群体演化而来,是云南的土著居民。利用PCR2RFL P 方法对这18 个土著民族进行Y染色体上13 个双等位基因位点进行基因分型。结果显示,不同历史族源的民族群体在Y染 色体双等位基因单倍型分布上具有一定的差异:在百越后裔民族群体中以单倍型H11 、H12 为主要分布;在氐羌后 裔民族中以单倍型H5 、H6 和H8 为主要分布;在百濮后裔民族群体中主要单倍型分布为H6 、H8 和H11 。进一步 主成分分析表明,百越后裔民族群体和氐羌后裔民族在主成分图上聚为两组,提示父系基因库有不同的来源,与历 史记载相印证。

Molecular modeling on some human interleukins sharing gamma c of interleukin-2 receptor, and structure-function relationships

Five models for human interleukin-7 (HIL-7), HIL-9, HIL-13, HIL-15 and HIL-17 have been generated by SYBYL software package. The primary models were optimized using molecular dynamics and molecular mechanics methods. The final models were optimized using a steepest descent algorithm and a subsequent conjugate gradient method. The complexes with these interleukins and the common gamma chain of interleukin-2 receptor (IL-2R) were constructed and subjected to energy minimization. We found residues, such as Gln127 and Tyr103, of the common gamma chain of IL-2R are very important. Other residues, e.g. Lys70, Asn128 and Glu162, are also significant. Four hydrophobic grooves and two hydrophilic sites converge at the active site triad of the gamma chain. The binding sites of these interleukins interaction with the common gamma chain exist in the first helical and/or the fourth helical domains. Therefore, we conclude that these interleukins binds to the common gamma chain of IL-2R by the first and the fourth helix domain. Especially at the binding sites of some residues (lysine, arginine, asparagine, glutamic acid and aspartic acid), with a discontinuous region of the common gamma chain of IL-2R, termed the interleukins binding sites (103-210). The study of these sites can be important for the development of new drugs. (C) 2000 Elsevier Science B.V. All rights reserved.

Interaction between human interleukin-16 and CD4 receptor of HIV-1

AIM: To study the interaction between human interleukin-16 (IL-16) and the receptor CD4 (T-lymphocyte differentiation antigen) of human immunodeficiency virus type 1 (HIV-1). METHODS: Two structurally con served regions (SCRs) of human IL-16 were built by the SYBYL/Biopolymer module using the corresponding transmembrane (TM) domain of human interleukin-1 (HIL-4) and HIL-2 as the templates. The coordinates for amino-terminal residue sequence, carboxyl-terminal residue sequences, and cytoplasm loops were generated using Biopolymer's LOOP SEARCH algorithm. RESULTS: HIL-16 first formed a homodimer, then contacted with CD4 dimer further forming a dimeric complex. Subsequently, the dimeric complex constructed the tetrameric complex by two disulfide bridges between the cysteines of HIL-16 (Cys31-Cys31). CONCLUSION: The interaction model is useful to propose the action mechanism of HIL-16 and is beneficial for rational designing of novel anti-HIV drugs.

南极中山站、长城站附近18个湖泊生态学特征的逐步判别分析

南极中山站、长城站附近１８个湖泊生态学特征的逐步判别分析蔡庆华，王洪铸，梁彦龄（中国科学院水生生物研究所，武汉４３００７２）关键词南极，湖泊，生态学特征，逐步判别分析，拉斯曼丘陵，乔治王岛ＳＴＥＰＷＩＳＥＤＩＳＣＲＩＭＩＮＡＮＴＡＮＡＬＹＳＩＳＦＯＲ...

无机阴离子在涂敷十六烷基吡啶的C_(18)键合相柱上的分离和测定

<正> 近年来,离子相互作用色谱法得到了迅速发展,使用这种方法,不仅可用有机树脂作固定相,同样可使用高效的键合固定相,并且可以通过改变离子相互作用,试剂在流动相中的浓度或涂敷在固定相上的量来改变柱的交换容量。实验使用常规的液相色谱仪,易于方法的广泛应用。在无机阴离子的分离中,这一方法显示了优越的分离效率。 本文研究了在涂敷十六烷基吡啶的C_8键合固定相上分离无机阴离子,并利用一些无机阴离子在

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

In this study, an IL-8 homologue has been cloned and identified from a reptile, Chinese soft-shelled turtle for the first time. The full-length cDNA of turtle IL-8 was 1188 bp and contained a 312 bp open reading frame (ORF) coding for a protein of 104 amino acids. The chemokine CXC domain, which contained Glu-Leu-Arg (ELR) motif and four cysteine residues, was well conserved in turtle IL-8. The 4924 bp genomic DNA of turtle IL-8 contained four exons and three introns. Phylogenetic analysis showed that the amino acid sequence of turtle IL-8 clustered together with birds. RT-PCR analysis showed that turtle IL-8 mRNA was constitutively expressed liver, spleen, kidney, heart, blood and intestine tissues of control turtles. Real-time quantitative PCR analysis further indicated that the turtle IL-8 mRNA expression was apparent in various tissues at 8 h and up-regulated significantly during 8 h-7 d after Aeromonas hydrophila infection. The present studies will help us to understand the evolution of IL-8 molecule and the inflammatory response mechanism in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

In this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved.

Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky)

Eighteen microsatellite markers were isolated and characterized using an enrichment protocol in the Chinese mandarin fish Siniperca chuatsi (Basilewsky), a commercially important piscivorous fish in China. Out of 48 pairs of primers designed, 18 loci exhibited polymorphism with three to six alleles (mean 4.4 alleles/locus) and average observed heterozygosity ranged from 0.633 to 0.833 (mean 0.748) in a test population from Dongting Lake of China. Except for two loci, all other 16 loci were in the Hardy-Weinberg equilibrium. These markers would be useful for such studies as population genetics, ecology and selective breeding of the Chinese mandarin fish in future.

Genomic organization, gene duplication, and expression analysis of interleukin-1 beta in channel catfish (Ictalurus punctatus)

Interleukin-1 beta (IL-1 beta) is one of the pivotal early response pro-inflammatory cytokines that enables organisms to respond to infection and induces a cascade of reactions leading to inflammation. In spite of its importance and two decades of studies in the mammalian species, genes encoding IL-1 beta were not identified from non-mammalian species until recently. Recent research, particularly with genomic approaches, has led to sequencing of IL-1 beta from many species. Clinical studies also Suggested IL-1 beta as an immunoreagulatory molecule potentially useful for enhancing vaccination. However, no IL-1 beta genes have been identified from channel catfish, the primary aquaculture species from the United States. In this study, we identified two distinct cDNAs encoding catfish IL-1 beta. Their encoding genes were identified, sequenced, and characterized. The catfish IL-1 beta genes were assigned to bacterial artificial chromosome (BAC) clones. Genomic studies indicated that the IL-1 beta genes were tandemly duplicated on the same chromosome. Phylogenetic analysis of various IL-1 beta genes indicated the possibility of recent species-specific gene duplications in channel catfish, and perhaps also in swine and carp. Expression analysis indicated that both IL-1 beta genes were expressed, but exhibited distinct expression profiles in various catfish tissues, and after bacterial infection with Edwardsiella ictaluri. (c) 2005 Elsevier Ltd. All rights reserved.

1×8阵列波导光栅型波分复用/解复用器设计的一种简单方法

用有效折射率法，采用近轴近似和波导模场分布的高斯近似，简化了阵列波导光栅(AWG)器件的设计过程中繁杂的计算，且保证了器件的性能指标。给出了设计思路，并给出了1×8路、中心波长为1550.9nm，波长间隔为1.6nm的AWG波分复用/解复用器的设计实例。