35 resultados para Economical profitabily

em Chinese Academy of Sciences Institutional Repositories Grid Portal


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The highly pure and active photosystem II (PSII) complex was isolated from Bangia fusco-purpurea (Dillw) Lyngb., an important economic red alga in China, through two steps of sucrose density gradient ultracentrifugation and characterized by the room absorption and fluorescence emission spectra, DCIP (2,6-dichloroindophenol) reduction, and oxygen evolution rates. The PSII complex from B. fusco-purpurea had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 nm) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Moreover, the acquired PSII complex displayed high oxygen evolution (139 mu mol O-2/(mg Chl h) in the presence of 2.5 mM 2,6-dimethybenzoqinone as an artificial acceptor and was active in photoreduction of DCIP (2,6-dichloroindophenol) by DPC (1,5-diphenylcarbazide) at 163 U/(mg Chl a h). SDS-PAGE also suggested that the purified PSII complex contained four intrinsic proteins (D1, D2, CP43, and CP47) and four extrinsic proteins (33-kD protein, 20-kD protein, cyt c-550, and 14-kD protein).

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Scanning electron microscopic (SEM) moire method was used to study the surface structure of three kinds of butterfly wings: Papilio maackii Menetries, Euploea midamus (Linnaeus), and Stichophthalma how-qua (Westwood). Gratings composed of curves with different orientations were found on scales. The planar characteristics of gratings and some other planar features of the surface structure of these wings were revealed, respectively, in terms of virtual strain. Experimental results demonstrate that SEM moire method is a simple, nonlocal, economical, effective technique for determining which grating exists on one whole scale, measuring the dimension and the whole planar structural character of the grating on each scale, as well as characterizing the relationship between gratings on different scales of each butterfly wing. Thus, the SEM moire method is a useful tool to assist with characterizing the structure of butterfly wings and explaining their excellent properties. (c) 2007 Optical Society of America.

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This paper deals with a case study of the restoration of submerged macrophytes for improving water quality in a hypertrophic shallow lake, Lake Donghu of Wuhan, Hubei Province, China. Macrophyte restoration experiments were conducted in large-scale enclosures established in three sublakes of different trophic status, and the effectiveness for water quality improvement was tested by using the enclosure experiment in the hypertrophic sublake. Water quality was remarkably improved after the reestablishment of aquatic macrophytes. It is suggested that the submerged vegetation of less polluted sublakes could be capable of recovering spontaneously once the stocking of herbivorous fishes has been ceased, and the K-selected plants such as Potamogeton maackianus should be introduced into these sublakes to enhance the stability of aquatic vegetation. However, it may not be possible and economical to restore the submerged macrophytes in severely polluted basins unless external pollution has been cut off and internal nutrient loadings considerably reduced. In this case, the r-selected submerged plants should be used as the pioneer species for macrophyte recovery. (C) 2001 Elsevier Science B.V. All rights reserved.

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Poly(3,4-ethylenedioxythiopliene):poly(styrene sulfonate) (PEDOT:PSS) films have been electrochemically polymerized in situ on ITO glass substrate in boron trifluoride diethyl etherate electrolyte (BFEE). Cyclic voltammograms show good redox activity and stability of the PEDOT films. These films had been directly used to fabricate organic-inorganic hybrid solar cells with the structure of ITO/PEDOT/ZnO:MDMC-PPV/Al. The solar cells made of electrochemically polymerized films exhibit higher energy conversion efficiencies compared with that prepared by the spin-coating method, and the highest value is 0.33%. This in-situ electropolymerized method effectively simplifies fabricating procedures and may blaze a facile and economical route for producing high-efficiency solar cells.

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Radiant heat conversion performance dominated by the active layer of Ga0.84In0.16As0.14Sb0.86 diode has been systematically investigated based on an analytic absorption spectrum, which is suggested here by numerically fitting the limited experimental data. For the concerned diode configuration, our calculation demonstrates that the optimal base doping is 3-4 x 10(17) cm(-3), which is less sensitive to the variation of the external radiation spectrum. Given the scarcity of the alloy elements, an economical device configuration of the 0.2-0.6 mu m emitter and the 4-6 mu m base would be particularly acceptable because the corresponding conversion efficiency cannot exhibit discouraging degradation in comparison to the one for the optimal structure, the thickness of which may be up to 10 mu m. More importantly, the method we suggested here to calculate alloy absorption can be easily transferred to other composition, thus bringing great convenience for design or optimization of the optoelectronic device formed by these alloys.

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In this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-DNBA)-degrading bacterium, Comamonas testosteroni A3, was investigated. Our results showed that the combinations of strain A3 with each of five strains with a high biofilm-forming capability (Pseudomonas sp. M8, Pseudomonas putida M9, Bacillus cereus M19, Pseudomonas plecoglossicida M21 and Aeromonas hydrophila M22) presented different levels of enhancement regarding biofilm-forming capability. Among these culture combinations, the 24-h dual-species biofilms established by C. testosteroni A3 with P. putida M9 and A. hydrophila M22 showed the strongest resistance to 3,5-DNBA shock loading, as demonstrated by six successive replacements with DMM2 synthetic wastewater. The degradation rates of 3,5-DNBA by these two culture combinations reached 63.3-91.6% and 70.7-89.4%, respectively, within 6 h of every replacement. Using the gfp-tagged strain M22 and confocal laser scanning microscopy, the immobilization of A3 cells in the dual-species biofilm was confirmed. We thus demonstrated that, during wastewater treatment processes, it is possible to immobilize degrader bacteria with bacteria with a high biofilm-forming capability and to enable them to develop into the mixed microbial flora. This may be a simple and economical method that represents a novel strategy for effective bioaugmentation.

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LUCC是全球变化研究的核心主题之一,也是社会经济可持续发展的关键问题。改革开放后四川的社会经济发展非常快,在各种因素的驱动下,土地利用/覆盖发生了深刻变化。目前四川省缺乏基于实际调查数据的、全域性的、具有连续时间序列的LUCC和驱动力分析及土地可持续利用研究成果,这对我们从全局上把握全省土地利用现状、发展变化趋势,利用土地政策参与宏观调控,实现长期可持续发展目标,建设资源节约型、环境友好型社会极为不利。本研究针对这一问题,选取全川八大土地利用类型作为研究对象,研究了全省1996年到2006年的土地利用/覆盖格局和变化情况,分析了不同尺度的驱动因素,对全省农用地和建设用地的集约利用状况、潜力进行了分析评价,并提出相应的对策措施。 1.1996年-2006年10年来整个省域的土地利用/覆盖格局变化。 (1)1996年-2006年全省的土地利用/覆盖格局 1996年,全省是一个以农用地为主的土地利用/覆盖格局,林地和牧草地属于优势覆盖类型(合占69.17%),居民点及工矿用地和交通用地合占只有3%左右。 2000年的LUCC格局较为明显的特点是耕地所占比重下降0.4个百分点,水域和未利用土地所占比重有所下降,牧草地保持不变,其余地类所占比重有所上升。 与2000年相比,2004年林草地的优势格局进一步得到强化(合占比重达到70.23%)。耕地面积占幅员面积的比重下降0.83个百分点,略有下降的有未利用土地、水域和牧草地。值得关注的是在“退耕还林还草”的大背景下,牧草地占幅员面积的比重下降0.04个百分点。 到2006年,仍为林草地为主导优势的格局,二者合占上升0.15%。在城市化快速推进的背景下,居民点及工矿用地中的城市用地和建制镇用地占比重超过15%,农村居民点占比重降至76%。交通用地中农村道路占比重降至57.8%,公路用地占比重升至37.5%。五个地貌区的土地利用/覆盖格局与全省的变化基本一致。值得关注的是盆西平原区的交通用地上升幅度和盆地丘陵区的未利用土地的开发利用力度明显大于其它地貌区。 (2)1996-2006年10年间土地利用/覆盖格局的变化 1996-2000年4年间,耕地、水域和未利用地三个地类下降,年均减少0.75、0.19和0.32个百分点。其中耕地年均减少49229.0公顷,约一半流向林地,13.77%流向园地,约20%流向建设用地。另外5个地类面积增长,增长绝对量最大的是林地,年均增长40063.7公顷,交通用地增幅最大,4年年均增长达1.95%。 2001-2004年是西部大开发逐步推进、“退耕还林还草”项目全面展开和土地整理深入实施的关键期,LUCC更为深刻。耕地、未利用地、水域和牧草地四个地类面积下降,其余地类按增长幅度依次是园地、交通用地、居民点及工矿用地和林地。耕地加速下降,年均降幅达到1.59%,其减少去向主要是林地(占66.75%)和园地(占19.84%),其增加来源主要是未利用地、园地和水域。交通用地的增幅最大,为3.96%,其增加主要来源于耕地、未利用土地和林地,分别占49.96%、16.63%和13.09%。居民点及工矿用地增长幅度为3.12%。 从1996年到2006年的10年间,耕地、未利用地、水域和牧草地下降幅度分别为10.36%、3.61%、1.34%和0.26%。园地增幅达23.61%。绝对面积增长最大的则是林地,达630733.3公顷。交通用地和居民点及工矿用地增幅也较大,分别为15.00%和9.31%。 10年间年均总变化量为310326.6公顷,2000年-2004年之间变化最大(为356865.8公顷),高于平均变化量,而1996-2000年间和2004-2006年间都小于平均变化量。 (3)10年间不同地貌区的LUCC变化 盆西平原区的特点是园地大幅上升达77%,居民点及工矿用地和交通用地也大幅上升,耕地、未利用地下降幅度大,该区耕地、水域、未利用地的减少强度和园地、居民点及工矿用地、交通用地的上升强度均居五区第一;盆地丘陵区的特点是牧草地下降幅度大,为-36.89%,交通用地、园地和林地上升幅度较大,该区耕地减少、未利用地减少、林地增加、居民点及工矿用地和交通用地增加的变化强度均居五区相应地类增减的第二位;盆周山地区的特点是耕地减少较多,交通用地和园地增长较大,该区林地变化强度居各区第一位,牧草地和水域变化强度居各区第二位,耕地、居民点及工矿用地和未利用地居各区第三位;川西南山地区的特点是园地、耕地、交通用地和居民点及工矿用地变化幅度大,另外四个地类变化较小。该区减少的牧草地占全省牧草地减少的97.91%,变化强度居各个地貌区的第一位,园地相对变化强度居五区的第二位;川西北高山高原区的特点是耕地大幅下降、园地大幅上升,交通用地升幅也较大,其余地类变化不大。值得注意的是,该区牧草地和水域面积增加,与全省该地类的变化相反。其余地类的相对变化强度均是五个地貌区中最小的。 用变化强度分值考量变化强度,盆西平原区的变化强度最大,盆地丘陵区和盆周山地区的变化强度相当,川西北高山高原区的变化强度则要小得多。 (4)1996年及2006年全省土地利用/覆盖格局的景观生态学分析 全省是以自然景观占优势(占约70%)、农业景观为补充、建设用地景观居于从属地位的土地利用景观格局。景观多样性和均匀度不高。到2006年,全省总的景观格局并无大的改变。总体情况是随着时间的推移和人类活动的加强,区内景观优势度上升、多样性和均匀度变小。但斑块数减少,斑块面积和斑块孔隙度有所增大。斑块的形状指数和分维数均有所下降,表明受人为干扰有加剧的趋势。反映景观格局结构的破碎度指数有轻微下降。景观指数的变化表明全省土地利用有缓慢集中、规模聚集的趋势。 (5)三大生态建设工程对土地利用/覆盖变化的影响 1996-2006年间LUCC与三大生态建设工程实施的耦合分析,发现退耕工程对耕地、林地、牧草地等地类覆盖变化的影响最大,天保工程次之,长防工程最小。 2.四川省LUCC驱动力分析 (1)总体分析: 从整体上分析,人为因素对区域整体LUCC的影响从1996年的63.32%增加到2006年的66.99%,变得日益强烈。同时人为因素影响强度表现出明显的区域差异,地势平缓、经济区位条件好的区域其人为影响强度明显较高。 政策体制转变下的经济高速增长、快速的城市化、工业化过程和生态建设是四川省LUCC宏观尺度的驱动因素。区域的LUCC主要受到了由内向外(从城市到乡村)和由外向内(从山顶向平地)两种作用力的共同推动。局部尺度上,如距离交通线、水利线、中心城市的远近,地形凸起、大型独立项目落址、重污染项目的阻隔等,甚至一些乡规民俗等因素也会成为LUCC的驱动影响因素。在较小的尺度上,人类个体行为选择对LUCC的影响也是存在的。   根据驱动因子的特性作者将其划分为驱变、阻变、良性、惰性因子等类型。 (2)分地貌区的驱动因子分析 各地貌区都存在城市化、工业化、生态工程实施、自然灾害等驱动因子,但主次不一。对于盆西平原和盆地丘陵区,城市化、工业化是前两位的因子,而对另外三个地貌区,生态工程实施和产业结构调整则成为第第一、二位的驱动因子。 (3)分地类的驱动因子分析(以坡耕地为例) 分坡度的耕地变化分析发现,耕地减少主要集中在2°以下的平地、15°-25°和25°以上三个坡度级,是其它坡度级耕地减幅的三倍左右。这表明耕地减少受城市化进程和“退耕还林还草”工程驱动影响尤为巨大。 3.土地利用格局优化、集约利用评价和可持续利用及对策研究 (1)土地利用格局优化的战略选择及调整预测 土地利用格局调整的战略是农业生产用地、建设用地和生态及其他用地占幅员的比重分别稳定在13%、7%和80%左右,重点是三大类别内部二级和三级地类的合理调整。 (2)全省土地集约利用评价 全省农用地利用集约度为0.46,总体上集约度不高,处于较适度利用阶段。建设用地利用集约度为0.38,处于较适度利用阶段。集约利用提升空间较大。 农用地的潜力主要在于加强土地保育、完善利用制度、提高单产。城市建设用地的包括存量潜力、强度潜力、结构潜力,空间很大。农村居民点整理潜力可以逐步挖掘。 (3)新增建设用地集约利用的统筹安排 据测算,到2020年,四川省城市建设用地需求量在463850-492360hm2之间,城镇各业新增建设用地规模为361276.79hm2,占用耕地200565.94 hm2。2004-2020年间四川省农村居民点整理潜力33.86万hm2。农村居民点建设用地需求量为70.57万公顷。 (4)土地集约利用措施与坡耕地可持续利用战略 提出了土地集约利用的措施。在对坡耕地生态系统结构与功能分析的基础上,提出坡耕地可持续利用战略与生态恢复战略,并从技术和政策层面提出了坡耕地合理利用和生态退耕的措施和建议。 LUCC is one of the key questions of global change and sustainable development of society. After the opening and reform of China, the society and economy of Sichuan Province developed very fast ,the land-use/cover changed very strong droved by many factors .But nowadays we have no constant spatial-temporal study and driving force analysis about the whole province based on investigation. And it is lack of land sustainable utilization study based on correlative study. So we choose all the land resource in Sichuan, combine RS and GIS and field investigation, and take statistic-mathematic means and system analysis, to study the LUCC patterns and different scale driving force of different physiognomy regions, land cover types and periods; to analyze the current situation and potential of land resource intensive utilization, and gave out corresponding measurements. We found that forest and grassland are the dominant cover types of Sichuan provincial land –use/cover pattern, and becoming more and more stronger from 1996 to 2006,the natural landscape is the metric and occupy 70%,the diversity and evenness index are not high; the totally change quantity from 2000 to 2004 is the biggest; cultivated land especially steep cultivated land ,garden plot, forestry land ,settlement and industry land and traffic land changed relative stronger; among five physiognomy regions ,the changing intensity of PEN XI PING YUAN QU is the biggest, CHUAN XI BEI GAO SHAN GAO QU is smallest; under the background of policy system changing, the fast developing of economy, fast urbanization and industrialization and ecology construction are the macro-scale driving force of Sichuan provincial LUCC; to compare the impacts of “TUI GENG GONG CHENG” on LUCC especially to cultivated land ,forestry land and grassland is strongest, “TIAN BAO GONG CHENG ” is stronger,“ HANG FANG GONG CHENG” is smallest; the intensive utilization level of farmland and construction land of whole province is relative moderation, there is huge potential to excavate and fulfill the increasing demand of construction land;we must take synthetic measurements to accelerate the sustainable utilization of land resource, including administrative, economical ,technological and ecological policies.

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海拔梯度造成的环境异质性,如崎岖的地形、复杂的植被结构以及花期延迟等可能会极大地影响到物种的形态和遗传变异格局。理解物种形态和遗传变异的海拔格局对于物种多样性的管理和保护是非常重要的。尽管植物群体遗传学是一个飞速发展的研究领域,然而与海拔相关的形态变异、遗传变异及群体间遗传差异的研究却很少。到目前为止,还不清楚遗传变异与海拔之间是否必然的相关性。 川滇高山栎是一种重要的生态和经济型树种,广泛分布于中国西南的四川、西藏、贵州和云南省的高海拔地区,在保持水土、调节气候方面起着十分重要的作用。尽管主要受阳光限制而仅分布于阳坡,但其海拔梯度范围较大,表明川滇高山栎对不同的环境具有很强的适应性。本文通过叶型及生理响应、微卫星分子标记和扩增性片段长度多态性方法,试图探索川滇高山栎叶沿海拔梯度的形态和生理响应及其沿海拔梯度的遗传变异格局,为川滇高山栎的保护和利用提供进一步的遗传学理论依据和技术指导。 对叶形、含氮量及碳同位素的试验结果表明,平均比叶面积、气孔密度、气孔长度和气孔指数等气孔参数随海拔的升高呈非线性变化。在海拔大于2800 m时,川滇高山栎的比叶面积、气孔长度和气孔指数都随海拔升高而降低,但是在海拔小于2800 m时,这些指标都随海拔的升高而增大。相对而言,单位叶面积的含氮量和碳同位素则表现出相反的变化模式。另外,比叶面积是决定碳同位素沿海拔梯度变化的最重要参数。本研究结果表明,海拔2800 m附近是川滇高山栎生长和发育的最适地带,在这里生长的植物叶片厚度更薄、气孔更大、叶碳同位素值更小。 利用六对微卫星引物对五个不同海拔川滇高山栎群体遗传多样性进行研究,结果表明,群体内表现出较高的遗传多样性,平均每位点等位基因数11.33个,平均期望杂合度达0.820。群体间差异较小,分化仅为6.6%。聚类分析也并没有显示出明显的海拔格局。然而低频率等位基因却与海拔呈显著性正相关(R2=0.97, P < 0.01),表明在高海拔处,川滇高山栎以更多的稀有基因来适应恶劣的环境条件。本试验结果表明由海拔梯度形成的选择性压力对川滇高山栎群体的遗传变异影响并不明显。 为了进一步探讨川滇高山栎群体遗传变异与海拔之间的相互关系,我们还对其进行了扩增性片段长度多态性分析。结果表明:(1)随海拔的升高(从群体WL2到群体WL5),群体内遗传变异降低,而群体间遗传差异增加;(2)低海拔群体WL1表现出最低的遗传变异性(HE = 0.181),同时与其余四个群体间呈现出最大的遗传差异性(平均FST = 0.0596);(3)在除去低海拔群体WL1后,Mantel检测表明群体间遗传距离与海拔距离之间表现出正相关性。另外,研究结果还表明,遗传变异受生境条件(过度的湿热环境)及人为干扰(火烧、砍伐和放牧)的影响,这一点至少在低海拔群体WL1上发生了作用。 通过叶形态、生理及DNA分子水平的研究,结果表明叶形态特征和碳同位素与海拔紧密相关,与海拔之间呈非线性变化,海拔2,800 m附近是川滇高山栎生长和发育的最适地带。海拔梯度在一定程度上会影响到川滇高山栎群体的遗传变异结构,但在这样一个狭窄的地理分布区域里,这种影响并不足以导致群体间较大的遗传分化。同时生境条件及人为干扰也是影响遗传变异的限制性因子,不容忽视。 Altitudinal gradients impose heterogeneous environmental conditions, such as rugged topography, a complex pattern of vegetation and flowering delay, and they likely furthermore markedly affect the morphological and genetic variation pattern of a species. Understanding altitudinal pattern of morphological and genetic variation at a species is important for the management and conservation of species diversity. Although plant population genetics is a fast growing field of research, there are only few recent investigations, which analyzed the genetic differentiation and changes of intra-population variation along altitudinal gradients. At present, it is still unclear whether there are some common patterns of morphological and genetic variation with altitude. Quercus aquifolioides Rehder & E.H. Wilson, which is an important ecological and economical endemic woody plant species, is widely distributed in the Yunnan and Sichuan provinces, Southwest China. Its large range of habitat across different altitudes implies strong adaptation to different environments, although it is mainly restricted to sunny, south facing slopes. It plays a very important role in preventing soil erosion, soil water loss and regulating climate, as well as in retaining ecological stability. In this paper, we tried to understand the altitudinal pattern of morphological and genetic variation along altitudinal gradients through the experiments of leaf morphological and physiological responses, microsatellite analysis and AFLP markers. In leaf morphological and physiological responses experiment, we measured leaf morphology, nitrogen content and carbon isotope composition (as an indicator of water use efficiency) of Q. aquifolioides along an altitudinal gradient. We found that these leaf morphological and physiological responses to altitudinal gradients were non-linear with increasing altitude. Specific leaf area, stomatal length and index increased with increasing altitude below 2,800 m, but decreased with increasing altitude above 2,800 m. In contrast, leaf nitrogen content per unit area and carbon isotope composition showed opposite change patterns. Specific leaf area seemed to be the most important parameter that determined the carbon isotope composition along the altitudinal gradient. Our results suggest that near 2,800 m in altitude could be the optimum zone for growth and development of Q. aquifolioides, and highlight the importance of the influence of altitude in research on plant physiological ecology. Genetic variation and differentiation were investigated among five natural populations of Q. aquifolioides occurring along an altitudinal gradient that varied from 2,000 to 3,600 m above sea level in the Wolong Natural Reserve of China, by analyzing variation at six microsatellite loci. The results showed that the populations were characterized by relatively high intra-population variation with the average number of alleles equaling 11.33 per locus and the average expected heterozygosity (HE) being 0.779. The amount of genetic variation varied only little among populations, which suggests that the influence of altitude factors on microsatellite variation is limited. However, there is a significantly positive correlation between altitude and the number of low-frequency alleles (R2=0.97, P < 0.01), which indicates that Q. aquifolioides from high altitudes has more unique variation, possibly enabling adaptation to severe conditions. F statistics showed the presence of a slight deficiency of heterozygosity (FIS=0.136) and a low level of differentiation among populations (FST=0.066). The result of the cluster analysis demonstrates that the grouping of populations does not correspond to the altitude of the populations. Based on the available data, it is likely that the selective forces related to altitude are not strong enough to significantly differentiate the populations of Q. aquifolioides in terms of microsatellite variation. To further elucidate genetic variation pattern of Q. aquifolioides populations under sub-alpine environments, genetic variation and differentiation were investigated along altitudinal gradients using AFLP markers. The altitudinal populations with an average altitude interval of 400 m, i.e. WL1, WL2, WL3, WL4 and WL5, correspond to the altitudes 2,000, 2,400, 2,800, 3,200 and 3,600 m, respectively. Our results were as follows: (i) decreasing genetic variation (ranging from 0.253 to 0.210) and increasing genetic differentiation with altitude were obtained from the WL2 to the WL5 population; (ii) the WL1 population showed the lowest genetic variation (HE = 0.181) and the highest genetic differentiation (average FST = 0.0596) with the other four populations; (iii) the positive correlation was obtained using Mantel tests between genetic and altitude distances except for the WL1 population. Our results suggest that altitudinal gradients may have influenced the genetic variation pattern of Q. aquifolioides populations to some extent. In addition, habitat environments (unfavorable wet and hot conditions) and human disturbances (burning, grazing and felling) were possible influencing factors, especially to the low-altitude WL1 population. The present study shows that there were close correlations between morphological features and carbon isotope composition in our data. This indicates that a coordinated plant response modified these parameters simultaneously across different altitudes. Around 2,800 m altitude there seems to be an optimum zone for growth and development of Q. aquifolioides, as indicated by thinner leaves, larger stomata and more negative d13C values. All available evidence indicates altitudinal gradients may have influenced the genetic variation pattern of Q. aquifolioides to some extent. Decreasing genetic variation and increasing genetic differentiation with altitude was obtained except for the WL1 population. And the environment of habitats and human disturbances were also contributing factors, which impact genetic variation pattern, especially to the low-altitude WL1 population.

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小麦条锈病(Puccinia striiformis f. sp. tritici)是世界性小麦病害,可导致受害小麦减产30%以上,甚至绝收。小麦条锈病在我国西南、华北麦区危害严重,四川麦区是小麦条锈病发病最重的地区之一,每年因条锈病流行造成小麦产量损失巨大。利用抗条锈病品种是控制该病害最安全、经济的有效途径,因此挖掘利用抗病新基因,开展抗病遗传基础研究是当前育种工作中面临的重要任务。 偏凸山羊草(Aegilops ventricosa,DDMvMv,2n=28)是一年生草本植物,起源于地中海西部沿岸地区,具有对小麦白粉病、锈病等高抗或免疫、耐盐、抗寒、蛋白质含量高等优良性状,是小麦遗传育种很好的种质资源。本研究以高抗条锈病的小麦—偏凸山羊草6Mv/6B代换系(Moisson 6Mv/6B)为材料,对其含有的带条锈病抗性基因的偏凸山羊草6Mv染色体在四川小麦背景中的传递情况、与小麦—簇毛麦双端体附加系所具有的白粉病抗性的聚合以及对Moisson 6Mv/6B进行电离辐射诱变筛选抗条锈病的小麦—偏凸山羊草易位系三个方面进行了研究。取得的主要研究结果如下: 1. Moisson 6Mv/6B与高感条锈病的四川地区普通小麦品种绵阳26、绵阳93-124和SW3243的杂种F1与其普通小麦亲本分别作为父、母本回交,通过对其BC1和F2的结实率、根尖细胞有丝分裂中期染色体的观察以及对条锈病抗性的鉴定,发现含6Mv染色体的F1植株作母本时的回交结实率(83.10%)普遍高于含6Mv染色体的F1植株作父本(48.61%),结实率与普通小麦基因型密切相关(χ2=34.15>>χ20.05=5.99(df=2));6Mv染色体在三种四川小麦中通过雌、雄配子传递的传递方式与其传递率间没有显著相关性,其传递率与普通小麦基因型呈显著相关性(χ2=6.42>χ20.05=5.99(df=2))。 2. Moisson 6Mv/6B与高抗白粉病的小麦—簇毛麦双端体附加系Pana(2n=42+2t)正反杂交,希望在聚合两者抗性的同时观察不同受体背景下的抗性反应。对Moisson 6Mv/6B和Pana正反杂交的结实率、杂交后代的农艺性状进行观察,并对杂交后代进行基因组荧光原位杂交(GISH)分析及条锈病和白粉病的抗性鉴定。结果表明Moisson 6Mv/6B作母本时杂交结实率(80.56%)高于Pana作母本时(58.33%),结实率与杂交方式间紧密相关(χ2=4.96>χ20.05=3.84(df=1));Moisson 6Mv/6B和Pana杂交后代株高比最高亲本高约10cm,成熟期也较两亲本提前两个星期左右;正反杂交后代中具有偏凸山羊草6Mv染色体的植株具有条锈病抗性,具有簇毛麦端体的植株具有白粉病抗性,同时筛选到4株含有偏凸山羊草和簇毛麦遗传物质并对条锈病和白粉病兼抗的材料,证明来自偏凸山羊草6Mv染色体的条锈病抗性与来自簇毛麦端体的白粉病抗性已经聚合在一起,且没有产生相互抑制的作用,暗示通过这两个抗性基因的聚合是完全能获得兼抗条锈病和白粉病的小麦新种质。 3. 对Moisson 6Mv/6B在减数分裂时期的成株进行总剂量为6Gy、辐射频率为120rad/min的60Co-γ射线辐射,对辐射植株自交后代进行农艺性状及根尖细胞有丝分裂中期染色体形态观察和条锈病抗性鉴定。结果为辐射植株自交结实率为2.22%,根尖细胞有丝分裂中期的染色体存在明显碎片,辐射自交后代植株对条锈病具有成株期抗性。 小麦—偏凸山羊草6Mv/6B代换系对条锈病抗性稳定,是培育条锈病抗性品种的良好供体。本研究证明在四川小麦背景中要利用该品种抗性,在结实数满足需要时,可将其作父本,亦可作母本,但关键是要选择好一个优良的受体基因型;同时其条锈病抗性与来自簇毛麦的白粉病抗性没有相互抑制作用,可将两者抗性有效聚合用于小麦育种中。 Wheat stripe rust (Puccinia striiformis f. sp. Tritici) is a worldwide disease of wheat, and could lead to victims of 30 percent or even total destruction of wheat production. Wheat stripe rust harms badly in China's southwest and North China. Sichuan province is one of the regions damaged by wheat stripe rust heavily. The use of resistant varieties is the most secure and economical way to control the wheat stripe rust. Therefore, it is essential to identify new disease-resistant genes and genetically research of disease resistance. Aegilops ventricosa (DDMvMv, 2n = 28) is an annual herbaceous plant, originating in the coastal areas of the western Mediterranean, with good characters such as resistance of wheat powdery, rust, salt, cold and high protein content. It is a good germplasm resource. In this study, the wheat- Aegilops ventricosa 6Mv/6B substitution line Moisson 6Mv/6B (highly resistant to the wheat stripe rust) was used to study on the transmission of chromosome 6Mv of Aegilops ventricosa in different genetic background of Sichuan wheat varieties, hybridization with wheat- Haynaldia villosa ditelosomic addition line Pana (highly resistant to the powdery mildew) and screening of wheat- Aegilops ventricosa translocation line by exposuring Moisson 6Mv/6B under ionizing radiation. The main results are as following: 1. Moisson 6Mv/6B was crossed with Sichuan wheat varieties mianyang26, mianyang93-124 and SW3243 (highly susceptible to stripe rust), respectively. Their F1 hybrids were further backcrossed as male and female to corresponding wheat varieties. The seed-setting rate, chromosomes confirmation in the mitotic metaphase of root tip cells, and resistance to stripe rust of the subsequent BC1 and F2 plants were investigated. The average seed-setting rate of backcross via 6Mv as female donor (83.10%) was higher than that of backcross via 6Mv as male donor (48.61%), suggesting that the seed-setting rate was associated with the wheat genotypes(χ2=34.15>>χ20.05=5.99(df=2)). In all analyzed populations, transmission frequencies of chromosome 6Mv were not correlated with the ways of 6Mv through male or through female. However, transmission frequencies of chromosome 6Mv were significantly correlated with Sichuan wheat genotypes(χ2=6.42>χ20.05=5.99(df=2)). 2. To aggregating the resistances to stripe rust and powdery mildew, as well as research on the resistance reactions in different genetic background, Moisson 6Mv/6B was reciprocally hybrided with the wheat- Haynaldia villosa ditelosomic addition line Pana (highly resistant to the powdery mildew). The seed-setting rate, agronomic characters, genomic in situ hybridization (GISH) of hybrid progenies,and resistances to stripe rust and powdery mildew were investigated. The results showed that the seed-setting rate of hybridization via Moisson 6Mv/6B as female donor (80.56%) was significant higher than that via Pana as female donor (58.33%). The seed-setting rate was associated with the hybrid methods (χ2 = 4.96> χ20.05 = 3.84 (df = 1)). The plant height of hybrid progenies was about 10 cm higher than Pana, the parent with maximum height. And the maturity of hybrid progenies was about two weeks earlier than that of the parents. In the hybrid progenies, the plants with the 6Mv chromosome have the resistance to stripe rust and the plants with the telosome from Haynaldia villosa have the resistance to powdery mildew. It was found that four plants with both the 6Mv chromosome and the telosome from Haynaldia villosa were resistant to stripe rust and powdery mildew. It indicated that the resistance to stripe rust and powdery mildew aggregated, and no mutual inhibition was found. It implied that the aggregation of the two resistance genes was able to provide the new wheat germplasm with the resistances to stripe rust and powdery mildew. 3. Moisson 6Mv/6B was irradiated with 60Co-γ rays of 6Gy (120rad/min) during meiosis. The agronomic characters and chromosomes confirmation in the mitotic metaphase of root tip cells,as well as resistance to stripe rust were investigated. The seed-setting rate of irradiated plants was only 2.22%. The chromosomes in mitotic metaphase had clear fragments. The resistance to stripe rust of progeny of irradiated plants was the adult-plant resistance. The wheat- Aegilops ventricosa 6Mv/6B substitution line is a good stripe rust resistance donor for its stabile resistance. Our study demonstrated that the key for use the resistance is to choose a good receptor. There is no difference between Moisson 6Mv/6B be the female and be the male if the seed number meets the requirement. At the same time, the stripe rust resistance of Moisson 6Mv/6B did not have the mutual inhibition with the powdery mildew resistance from Haynaldia villosa. It is able to aggregate the two resistances for wheat breeding.

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猪场废水COD浓度高、氨氮浓度高、悬浮物浓度高,已成为农村面源污染的主要来源,并严重威胁到农村饮用水安全。猪场废水氨氮浓度高、处理难度大,如何采用经济高效的方法,去除氨氮使其达到排放标准,一直是猪场废水处理中面临的重要难题。 厌氧氨氧化是近年受到国内外水处理研究者广泛关注的新型生物脱氮技术,具有不需要外加有机碳源、节省供氧量、降低能耗等优点。虽然国内外研究者对厌氧氨氧化过程的脱氮机理、厌氧氨氧化菌的生理生化特性等进行了多方面的研究,但已有的报道大多以模拟废水为研究对象,以猪场废水为研究对象的报道,在国内外文献中极少有报导。 本论文以猪场废水为主要研究对象,考察了猪场废水的亚硝化过程、厌氧氨氧化的启动过程,并对亚硝化和厌氧氨氧化联合用于猪场废水脱氮进行了探索。 1.论文首先研究了猪场废水的亚硝化过程,考察了废水水质和主要运行条件对亚硝化过程的影响。实验表明:(1)亚硝化阶段反应时间为8到10h时,出水中氨氮和亚硝酸盐浓度比可达到1:1~1:1.23,满足厌氧氨氧化反应对二者比例的要求;达到前述要求时,氨氮去除率达到58.3~65.6 %,亚硝化率在整个过程均保持在97 %以上,COD去除率在59.2~68.6 %;(2)曝气量(溶解氧)对亚硝化过程影响显著,随着曝气量增大,达到厌氧氨氧化要求的氨氮与亚硝酸盐氮浓度比例所需水力停留时间τ越短,pH出现明显下降的时间越短;(3)τ对应的pH在7.8~8.1之间,无需进行pH调节即可满足厌氧氨氧化反应对pH的要求;(4)氨氮和COD降解过程遵循一级反应动力学,氨氮和COD降解的速率常数分别为0.0656~0.0724 1/h和0.0491~0.0664 1/h。 2.在进行亚硝化过程研究的同时,以模拟废水为试验对象,进行厌氧氨氧化启动研究。以反硝化污泥和养殖厂储水池厌氧底泥的混合污泥作为接种污泥,历时大约100天,培育出具有厌氧氨氧化活性的污泥,氨氮和亚硝酸盐氮最高进水浓度分别为223.8 mg/L和171.4 mg/L,去除率最高分别达48%和41.5%,此时二者消耗比例为1.33:1。 3.在猪场废水的亚硝化研究完成和厌氧氨氧化过程初步启动成功后,在模拟废水中逐步加入猪场废水的亚硝化处理出水,逐步实现亚硝化和厌氧氨氧化的组合。亚硝化出水添加到厌氧反应器后,厌氧氨氧化反应仍可继续进行,且去除效率逐步提高。研究发现添加的亚硝化出水中携带的亚硝化细菌在厌氧氨氧化菌膜外层生长并累积,增加了厌氧氨氧化反应基质的传质阻力,妨碍了厌氧氨氧化效率的提高。 4.亚硝化-厌氧氨氧化实际工程应用探索中,生物接触氧化池可在有效去除废水中的有机物的同时实现亚硝化,出水中氨氮和亚硝酸盐比例平均为1.10,可满足后续厌氧氨氧化的要求;在适宜的进水浓度和温度下,ABR池出现了厌氧氨氧化启动的迹象;研究同时发现,水质的波动和气温的变化是工程中影响厌氧氨氧化菌活性的重要因素。 论文的主要创新点在于:(1)以猪场废水为研究对象,以实现厌氧氨氧化为目标,对亚硝化过程进行了比较详细的考察,获得了亚硝化出水满足厌氧氨氧化要求的工艺条件,通过对其COD和氨氮降解过程的考察,得出亚硝化阶段COD降解和氨氮去除的动力学模型;(2)对亚硝化-厌氧氨氧化处理猪场废水进行了探索,确立了影响其污染物去除率稳定的重要因素。 论文的上述研究成果,为厌氧氨氧化技术的实用性研究提供理论依据。 Piggery wastewater, which is characterized by high concentration of COD、ammonium and suspend substance, has become a most important source of non-point source pollution and also severely threats drinking water security in rural area. How to discharge piggery wastewater with the ammonium concentration meeting standard by economical and effective method? This is the most urgent problem in piggery wastewater treatment. As a new biological nitrogen removal technology, Anammox process has been paid more and more attention by researchers all over the world. Anammox has advantages of no need of organic carbon addition, low oxygen consumption and energy consumption. Plenty of investigations have been carried out to the mechanism, physiological and biochemical characteristic of bacteria about Anammox. Most of researches focused on synthetic wastewater, there is rare report about its application in piggery wastewater. In this paper,experimental studies were performed to investigate Sharon process in treatment of piggery wastewater,the start up process of Annammox using synthetic wastewater were studied, the feasibility of applying Sharon-Anammox process in the nitrogen removal of piggery wastewater was evaluated. 1. Sharon process of piggery wastewater was firstly investigated to analyze the effects of water quality and main running parameters, which meet the NH4+-N to NO2--N ratio requirement of successive Anammox. Results showed: (1)During Sharon Process,after 8~10 hours’ reaction the NH4+-N to NO2--N ratio in effluent reached 1:1.0~1:1.23, when the removal percentage of NH4+-N was 58.3~65.6 %, a semi-nitration rate of above 97 % was achieved during the process; meanwhile 59.2~68.6 % of the COD was also removed. (2)The aeration rate(oxygen) had obvious effect on the hydraulic retention time(τ) which met the NH4+-N to NO2--N ratio requirement of Anammox. As aeration rate increased, the hydraulic retention time(τ) was shortened. (3) The pH corresponding to τ was between 7.8 and 8.1, thus it needed no artificial adjustment. (4) The reduction of ammonia and COD followed the first-order reaction kinetics. The velocity constants of ammonia and COD were 0.0656~0.0724 1/h and 0.0491~0.0664 1/h, respectively. 2. The startup of Anammox process using the artificial wastewater was performed simultaneously with Sharon. The aim was to investigate the running parameters of Anammox and make foundation for the combination stage. By using the mixture of denitrifying sludge and anaerobic sludge in tank of the breeding factory, sludge of Anammox activity was cultivated in UASB after 100 days. The removal percentage of NH4+-N and NO2-N were up to 48% and 41.5%, respectively, when the NH4+-N and NO2-N influent concentration were 223.8 mg/L and 171.4 mg/L, respectively, the NH4+-N and NO2-N removal rate was 1.33:1. 3. After investigation of Sharon and startup of Anammox, effluent of Sharon process was added into the synthetic wastewater to combine Sharon and Anammox step by step. It took some time after the addition of Sharon effluent that Anammox reaction continued and the removal rate kept increasing. It indicated that nitrifying bacteria were carried by the Sharon effluent cumulated in the outer layer of Anammox. This enhanced transfer resistance of Anammox reaction and the increasing removal rate was restrained. 4. In the bio-contact oxidation pond of practical project, Sharon process were carried out successfully and organic compounds were removed effectively. An average NO2-N/ NH4+-N rate of 1:1.0 was achieved in the effluent, which met the requirement of successive Anammox. Under condition of suitable influent concentration and temperature, there was evidence that Anammox could start up in ABR. The variety of wastewater and temperature had great affects on Anammox activity in practical engineering. Innovation of this paper: (1) The Sharon process for treating piggery wastewater was discussed in details. Technological parameters that met requirement of Anammox were obtained. The dynamic models of COD and ammonium removal in the process were educed. (2) Sharon-Ananmmox for treatment of piggery wastewater was investigated, and the primary influencing factors was studied. This paper could be a theoretical consult for research of Anammox utility.

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钻井废水是油气井开采钻探过程中产生的废水,钻井废水成分复杂,有机物浓度高、色度高、悬浮物浓度高,水质变化大,排放点分散,不经处理排放会污染环境,破坏生态。随着石油工业的不断发展和国家环保法律法规的日益严格,钻井废水的治理也越来越受到重视。如何采用经济有效的方法处理废弃钻井液,对油气井开采业的可持续发展具有重要意义。本论文以遂宁磨153 井的钻井废水为主要研究对象,在对废水进行絮凝沉降预处理和生物法处理探索的基础上,针对钻井废水可生化性差的特点,采用水解酸化和Fenton 试剂改善钻井废水的可生化性,对反应过程进行了比较详细的考察,对可生化性改善的机理进行了探索。主要研究结论如下:1 用PFS 和PAC 配制的混合混凝剂对钻井废水COD 的去除效果比较显著,在最佳条件下COD 的去除率可达75%,且絮体沉降速度较快,出水pH 保持中性;2 水解酸化法处理钻井废水可显著改善废水的可生化性。经48 小时水解酸化处理,钻井废水的理论BOD5可提高约22 倍,表观BOD5/COD值由0.004 提高到0.034。用接触氧化反应器处理经水解酸化处理后的废水,处理效果比较稳定,COD平均去除率达35.5%;3 研究了Fenton反应中各影响因子对废水COD去除率、BOD5/COD的影响并分析其作用机制,确定了最佳条件:初始pH为4.0,H2O2/Fe2+(摩尔浓度比)为20,H2O2/COD(质量浓度比)为1,反应时间为2 个小时。此条件下,废水的COD去除率约为40%,BOD5/COD值从0.002~0.003 提高至0.15~0.2,可生化性得到很大提高。本论文的主要创新点在于:1 以成分复杂、水质变化大的气井钻井废水为研究对象,从理论BOD 和表观BOD 两方面对水解酸化过程中废水可生化性的变化进行了分析;2 对Fenton 试剂改善钻井废水可生化性的过程、主要影响因素进行了比较详细的考察。本论文的研究成果,可为生物法处理钻井废水的深入研究提供理论依据。Drilling wastewater is produced in the process of oil-gas well drilling,because of its complicated composition, high concentrate of organic compound andsuspended solid, high chroma, levity of water quality and decentralization ofdischarge point, it pollutes environment seriously if discharged without treatment.With the development of petroleum industry and the issuing of more strict laws forenvironmental protection, it has been paid more and more attention on drillingwastewater treatment. It is of great importance for the sustainable development ofoil-gas well drilling to treat drilling wastewater by economical and effective methods.In this paper, drilling wastewater of Mo No.153 well in Suining was studied asthe main object. On the basis of research on pre-treatment with flocculant andbiological treatment, and according to the character of poor biodegradability, thedrilling wastewater was treated by hydrolytic acidification and Fenton’s reagent toimprove its biodegradability. The process and mechanism of biodegradabilitychanging were investigated. The primary conclusions are:1 It is effective to treat drilling wastewater with mixing PFS and PAC asflocculant. The removal rates of COD came up to 75% under optimal conditions, thesedimentation rate of flocculation is rapid, and the pH value of treated water remainedneutral;2 The biodegradability of drilling wastewater was highly improved afterhydrolytic acidification process. The theoretic BOD5 of drilling wastewater increasedby 22 times and its detected BOD5/COD ratio increased from 0.004 to 0.034 afterhydrolytic acidification for 48 hours. The wastewater after hydrolytic acidificationwas treated by biological contact oxidation reactor. Stable treatment performance was achieved, and the average removal rates of COD came up to 35.5%;3 The effects of various affection factors on the removal efficiency of COD andBOD5/COD radio in treating drilling wastewater by Fenton’s reagent wereinvestigated and the mechanism was analyzed. The optimal conditions were: initialpH of solution was 4.0, the molar ratio of H2O2 and Fe2+ was 20, the concentrationratio of H2O2 and COD was 1 and the reaction time was 120 min. Under the aboveconditions, the removal efficiency was about 40% and the ratio of BOD5 and CODincreased from 0.002 ¡« 0.003 to 0.15 ¡« 0.2. The biodegradability of drillingwastewater was greatly improved.The innovations of this thesis are:1 The drilling wastewater was taken as the research object which hascomplicated composition and variational water quality, and the changes ofbiodegradability were analyzed from theoretic BOD and detected BOD aspects duringhydrolytic acidification process;2 The biodegradability changing process and primary affection factors of drillingwastewater treating by Fenton’s reagent were investigated.The results of this study could provide theoretic foundation for further researchon biological treatment of drilling wastewater.

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造纸行业是造成我国水环境有机污染物的重要污染源之一,其水污染的特点是小厂多、草浆多、工艺落后、污染扩散面广、造成废 水排放量大,每年排放的废水量约39亿立方米,占全国工业废水排放量的1/6,其中有机污染物(以BOD5计)160万吨左右,约占全 国工业废水中有机污染物总量的1/4。尤以占全国制浆造纸行业90%以上的碱法草浆造纸厂的蒸煮黑液量大面广,除含有机物外,还 含有木质素、残碱、硫化物、氯化物等污染物,属于PH值高、色度深、难于治理的高浓度有机废水,对水体污染特别严重,各地要 求治理呼声很高,急待研究并尽快找出各种有效的治理途径。对于碱法草浆蒸煮,黑液高浓度废水的治理,有各种方法,根据国内 的研究进展和我们已有试验工作表明,最经济有效,具有实用价值,在生产上可获得成功是厌氧处理法。近10多年来,国外关于高 效厌氧处理技术研究进展迅速,并出现了多种多样的工艺设备,如高效厌氧生物反应器,并在实用化方面取得了很大成绩,建立了 生产性装置,达到了高负荷运行,效果良好。本试验是根据我们已有研究基础,针对我国国情,对小型制浆造纸厂水污染防治除了 开发碱回收及各种综合利用技术外,要特别加强废水(废液)实用技术研究的指导思想,本试验采用改进型的上流式厌氧污泥床反应 器,设计了两种试验方案,通过试验结果如下。1. 试验方案I—碱法草浆黑液酸化和厌氧发酵I号UASB反应器动态模型试验结果表 明:(1). 采用中温35℃±1℃高效厌氧反应器USAB内装有填料(陶粒)和三相分离器,具有保持高浓度生物量和防止污泥流失的特点 ,污泥浓度Vs 可达30%以上,因而具有高效、节能、产能、滞留期短的优点,当进水CODcr在7500-10000mg/l,HRT由7天缩短到3天 ,有机容积负荷在1.22gCODcr/l·d-3.43gCODcr/l·d时,CODcr平均去除率可达55%-45.5%,最高CODcr去除率可达60.2-63.5%, BOD5去除率可达75.9-83.2%,沼气容积产气率可达0.29-0.67l/l·d,每克CODcr转化为沼气产率达0.39-0.48l/gCODcr·d,CH4含量 65.8-75.5%。厌氧发酵出水再用化学法进行后处理脱除难降解的木质素,CODcr总去除率达80%以上。(2). 动态试验结果表明:采 用酸化—厌氧发酵处理黑液工艺合理,技术路线可行。2. 试验方案II—黑液用化学法(Hcl)去除木质素进行厌氧发酵,II号UASB反 应器动态模型试验结果表明:(1). 采用中温35℃±1℃高效厌氧反应器UASB(内有软填料),当进水CODcr7000-13000mg/l左右,HRT 由6天缩短到1天,有机负荷由0.98gCODcr/l·d增加到11gCODcr/l·d时,COD平均去除率均可稳定在70-77%,BOD5去除率为87.3- 93.1%,沼气容积产气率0.21-2.6l/l·d,每克CODcr转化为沼气产率为0.39-0.48l/gCODcr·d,高的可达0.53l/gCODcr·d,转化 率较高,CH4含量63-70%。(2). 试验证明碱法草浆黑液物化预处理—厌氧发酵处理的技术路线也是可行的,工艺合理、效果较好。 在有条件的工厂可采用。3.厌氧发酵阶段几大类群微生物计数表明:(1). 当发酵工艺和运行处于相对稳定状态时,微生物群体的 组成也达到相对的稳定,各类微生物之间保持动态平衡关系。当产乙酸菌的数量为107-108个/ml时,产甲烷菌的数量为105-106 个/ml,当产乙酸菌数量为106-107个/ml时,产甲烷菌的数量为103-105个/ml。(2).稳态运行条件下,黑液预处理为甲烷发酵创造 了有利的生态环境,获得了较好的处理效果和较高的COD转化为沼气的产率0.39-0.48l/g·CODcr·d,反应器中形成较为稳定而数 量较下水污泥中高1-2个数量级的厌氧发酵微生物区系组成。这一结果为黑液厌氧发酵提供了微生物理论依据。Paper industry is one of the important pollution source of water environment in our country. Its character of water pollution is many small factories, much grass pulp, disadvantageous technique, large preading area of pullution. Its effluent makes up 1/6 of whole country's industry wastwater. Its organic pollutant accounts 1/4 of whole country's. Alkaline grass paper pulp effluent with pollutants such as ligoin, remaining alkali sulfide, chloride besides organic material, is a kind of high concentrate organic wastewater which has high PH walug, dark colour and is difficult in treatment. There is urgent require to find ways to treat the wastewater. There are different ways to treat alkaline paper grass pulp effluent. According to the research advances and our experiment work, the most economical and useful way is anaerobic degradation which was advanced quick in last ten years. In the control of waste water of small pulp paper mill, the study of wastewater utilization technology should be emphasized, besides alkaline retrieving and different kinds of comprehensive utilization technology. Our experiment used modified UASB(Upflow Anaerobic Sludge Blanket Reactor). Two kinds of plan were disgned. The results are lined below. 1. The first experiment plant-aciding black pulp effluent and methanogenic digestion. The dynamic model experiment results of I-UASB reactor showed: (1)The mesophilic(35℃±1℃)high effect UASB reactor having haydite and threee state seperation in it had the character of keeping high bioimass concentration and preventing losss of sludge. It had advantages of high effect, energe saving, energe prodcing and short HRT(Hydroulic retention time). When the influent COD was 7500-10000mg, HRT was shortened from 7 days to 3days, organic loading rate was 1.22g-3.43COD/l· d, the average COD removal efficiency was 55%-45%. The highest COD efficiency was 60.2-63.5%, BOD removal of 75.9 -83.4% was achieved. Biogass production rate were up to 0.29-0.67l/l·d. Biogass converted efficiency from every gram of COD could reach 0.39-0.48l/gCOD·d. Methane content was 65.0-75.5%. Chemical method was used to deplate lignin in anaerobic digestion effluent. Total COD removal efficiency could be more than 80%. (2)Using aciding annaerobic digestion to treat the black effluent was reseanable in technique and technology. 2. The second experiment plan-anaerobic digestion was used after the chemical method was used to deplate lignin in the black effluent. The result of dynamic experiment of II-UASB reactor showed: (1)High effect mesophilic (35℃±1℃)UASB reactor having soft slaffing in was used. When influent COD was about 7000-13000mg/l, HRT was shortened from 6 days to 1 day and organic loading rate was increased from 0.90 to 11g COD /l·d, average COD removal efficiency remained stable on 70-77%. BOD, removal efficiency was between 87.3-93.1%. Biogass production rate was 0.2-2.6l/l ·d .Biogass converted efficiency from a gram of COD was 0.39-0.481/gCOD·d with the high value of 0.53l/gCOD·d. Methane content was 63-70%. (2)The way that using physical, chemical Pre-treatment-anaerobic digestion to treat alkaline black effluent is feasible and can be used in some factories when the condition exists. 3. Counting of several class of microoganisms in anaerobic digestion stage showed: (1)As the disgestion was in stable motion, the compositon of microorganic colony could get relative stable. Dynamic balance was remaining among different kinds of microorganism such as methanogenic bacteria, Acidogenic bacteria, sulfate reducing bacteria, and heterotrophic bacteria. (2)Under stable motion, the pre-treatment of black effluent produced favourable eco-enviroment for methanegenic digestion. Good treatment effect and high biogass convertent efficiency from COD(0.39-0.48l/g·COD· d)were gotten. Some stable and high quantity(10-100times more than sewage sludge)microorganism colony were formed in the reactor. This result provided theoretical basis for anaerobic digestion of black effluent.

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近年来,我国炼油行业发展迅速,炼油能力全世界第二,炼油行业已成为污染大户。本研究针对炼油废水生物处理中存在的稳定达标难、抗冲击负荷能力差、建设投资与运行成本高等问题,就菌剂强化处理炼油废水中试与工程应用展开了研究,以期为菌剂的工程应用与推广提供理论参考与技术支持;并以炼油废水中的主要特征污染物苯酚为研究对象,考察了不同浓度苯酚冲击下功能菌的响应机制,并以此为指导研制功能菌激活促进剂,考察其对功能菌生物学指标的调控效果,以期为废水生物处理有毒污染物冲击调控提供理论依据与技术支持。 中试研究表明,菌剂强化处理炼油废水,出水COD、NH4+-N 平均值为86.7、7.6 mg/L,其平均去除率较常规生物处理系统分别提高了35.47%、59.28%,其耐受COD、NH4+-N 容积负荷分别高达2.42、0.139kg/(m3·d),具有良好的耐冲击能力。工程应用研究表明,菌剂强化处理炼油废水,出水COD、NH4+-N 平均值分别为85.05、8.4mg/L,其去除率较常规生物处理系统提高了25.1%、28.7%,出水水质各项指标均达到了国家《污水综合排放标准GB 8978-1996》一级排放标准。技术经济分析表明,菌剂强化处理炼油废水在建设成本、运行成本上分别降低38%、49%,具有良好的技术经济优势。 苯酚冲击下功能菌响应机制研究表明:不同浓度苯酚冲击下,生物学指标生物量、脱氢酶酶活、1,2-双加氧酶酶活对冲击都有不同程度的响应,其响应敏感程度为脱氢酶酶活>生物量>1,2-双加氧酶酶活。1,2-双加氧酶酶活与COD 降解率相关性良好,可表征苯酚降解过程,确认为调控重点。以此为指导研制出苯酚降解功能菌抗冲击激活促进剂,可有效调控功能菌对有毒污染物苯酚的降解效果,1000mg/L 苯酚冲击下,经调控,其COD 去除率较对照提高20%,降解时间缩短16%以上。其对生物学指标的调控效果为1,2-双加氧酶酶活>生物量>脱氢酶酶活,验证了功能菌在苯酚冲击下的响应机制。研究表明菌剂强化处理炼油废水切实可行,具有良好的技术经济优势。有毒污染物冲击下废水生物处理系统响应机制研究为抗冲击调控提供了新的研究思路。 Currently, China’s oil refining industry is developing rapidly and has become the second largest all over the world. The oil refining industry is one of the major pollution industries in our country. The pilot scale study and engineering application research were conducted aiming at the problems in refining wastewater such as poor treatment stability and water quality, poor anti-shock capacity and expensive running cost, etc., so as to provide theoretical references and technological supports for the engineering application and popularization of microbial preparation in wastewater treatment. Also, the response mechanism of functional microbe under shock of different phenol concentrations, which is the main pollutants in refinery wastewater, was studied. Based on this result, functional microbe activation accelerator was developed, and the regulation effect of functional microbe biological index under phenol shocking were studied, in order to provide theoretical basis and technological support for regulation of toxic shocking of wastewater biological treatment. The result of pilot scale research indicated: for treatment of refinery wastewater in bioaugmention treatment system of microbial preparation, the COD and NH4+-N average value of effluent was 86.7 and 7.6 mg/L, Comparing with normal biological treatment system, the average removal rates of COD, NH4+-N increased 35.47%,59.28% separately by bioaugmention treatment system, which showed better anti-shocking capacity, the volumetric load r of COD and NH4+-N reached 2.42 kg/(m3·d) and 0.139 kg/(m3·d), respectively. The research on engineering application of refinery wastewater bioaugmentation treatment by microbial preparation indicated:the average concentrations of effluent COD and NH4+-N in the bioaugmentation treatment system were 85.05 and 8.4mg/L, which increased by 25.1% and 28.7% comparing with normal biological treatment system of refinery wastewater, And the effluent quality meets the first grade of discharging standard of National Integrated Wastewater Discharge Standard GB 8978-1996. The economic analysis of technology indicated: the demonstration project of bioaugmentation treatment of refinery wastewater by microbial preparation decreased by 38% in construction cost and 49% in running cost. This technology has economic benefits. The response mechanism of functional microbe under phenol shock indicated: biological index such as the biomass concentration, dehydrogenase and 1,2-dioxygenase had different responses under phenol shocking of different concentrations. The response sensitivity of different biological index under phenol shocking of different concentrations is: dehydogenase activity > biomass >1,2-dioxygenase activity, and high correlation of 1,2-dioxygenase and COD degradation percentage is achieved, thus 1,2-dioxygenase could be used to reflect the degradation situation of pollutants. So, 1,2-dioxygenase is the keypoint of regulation. The anti-shock activation accelerator of phenol degradation functional microbe was primarily developed. The results indicated: the activation accelerator could regulate the degradation effect of toxic substance-phenol by functional microbe effectively. For the functional microbe treatment system under phenol shocking of 1000mg/L, the COD degradation rate increased by 20% and the degradation time reduced by more than 16% under regulation of activation accelerator. The regulation effects of biological index are: 1,2-dioxygenase > biomass > dehydrogenase. In this way, the response mechanism of functional microbe under toxic shocking is verified. The result indicated: the augmented microbial preparation treatment of refinery wastewater is applicable. It has many technical and economical advantages. The research results of responses mechanism of wastewater treatment system on toxic pollutants would offer a new idea for regulation of anti-shock.

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对氦(He)离子高温(600K)注入6H-SiC中的辐照缺陷,在阶梯温度退火后演化行为的拉曼光谱和室温光致发光谱的特征进行了分析.这两种方法的实验结果表明,离子注入所产生晶格损伤的程度与注入剂量有关;高温退火导致损伤的恢复,不同注入剂量造成的晶格损伤需要不同的退火温度才可恢复.在阶梯温度退火下呈现出了点缺陷的复合、氦-空位团的产生、氦泡的形核、长大等特性.研究表明:高温(600K)注入在一定剂量范围内是避免注入层非晶化的一个重要方法,为后续利用氦离子注入空腔掩埋层吸杂或者制备低成本、低缺陷密度的绝缘层上碳化硅(SiCOI)材料提供了可能.

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The Lanzhou All Permanent magnet ECR ion source NO. 1 (LAPECR1) is the first all permanent magnet multiple ECRIS made in IMP. This ECRIS is running at 14.5GHz and can provide intense low charge state ion beams (varying from several to hundreds of e mu A) or medium charge state ion beams (varying from several to tens of e mu A). The size of source body is circle divide 102mmx296mm, the compactness and economical features enable the source suitable to be put on a HV platform or equipped by a small laboratory. This article gives the main parameters of the ion source.