菜花烙铁头蛇毒中一种具有激肤释放酶与纤维蛋白原溶酶活性的Jerdonase

通过DEAF Sephadex t-50阴离子交换、超细Sephadex 6100分子筛和反相高效液相CQ色谱层析，从菜花烙铁头蛇毒冻干粉中纯化出一种具有激肤释放酶活性和。纤维蛋白原溶酶活性的丝氨酸蛋白酶，命名为Jerdonase。在12 .5%胶浓度的SD6还原电泳条件下，该酶分子量大约为55 kD，在非还原电泳条件下，分子量大约为53 kD。此酶是一种糖蛋白，含有约35 .8%的中性糖。它的N末端氨基酸序列为IIGGDEENINEHPFLVALYDA，其序列和蛇毒中其他丝氨酸蛋白酶具有非常高的序列相似性。Jerdonase能够催化BAEE、 S-2238和S-2302的水解，其水解活性可被PMSF抑制，但是EDTA对此没有影响。Jerdonase能优先水解人纤维蛋白原的An链，同时伴随有微弱的B[3链水解活性。另外，此酶能够水解牛低分子量的激肤原，释放舒缓激肤。总之，所有的结果表明Jerdonase是一个具有多功能活性的蛇毒丝氨酸蛋白酶。

Characterization of a thrombin-like enzyme from the venom of Trimeresurus jerdonii

From the venom of Trimeresurus jerdonii, a distinct thrombin-like enzyme, called jerdonobin. was purified by DEAF A-25 ion-exchange chromatography, Sephadex G-75 gel filtration, and fast protein liquid chromatography (FPLC). SDS-PAGE analysis of this enzyme shows that it consists of a single polypeptide chain with a molecular weight of 38,000. The NH2-terminal amino acid sequence of jerdonobin has great homology with venom thrombin-like enzymes documented. Jerdonobin is able to hydrolyze several chromogenic substrates. The enzyme directly clots fibrinogen with an activity of 217 NIH units/mg, The fibrinopeptides released, identified by HPLC consisted of fibrinopeptide A and a small amount of fibrinopepide B. The activities of the enzyme were inhibited by phenylmethylsulfonyl fluoride (PMSF) and p-nitrophenyl-p-guanidinobenzoate (NPGB). However, metal chelator (EDTA) had no effect on it. indicating it is venom serine protease. (C) 2000 Elsevier Science Ltd. All rights reserved.

烙铁头蛇毒L-氨基酸氧化酶的分离、纯化及其生物学活性

通过DEAF Sephadex A-50阴离子交换柱，Sephadex G75分子筛，Resourse Q阴离子交换柱三步层析从湖南产的烙铁头蛇毒中分离、纯化得到一个L-氨基酸氧化酶( T1vf LAO)，它由两个非共价的亚基组成，每个亚基的分子量为55 kD。与台湾产的烙铁头蛇毒L-氨基酸氧化酶分子量(70 kD)不同。T1v+ LAO的N末端氨基酸序列是ADNKNPLEECFRETNYEEFLEIAR，与报 道的蜂科的L-氨基酸氧化酶的相似性比眼镜蛇科的要高。T1vf LAO能抑制大肠杆菌、金黄色葡萄球菌和痢疾杆菌的生长，杀死肿瘤细胞以及诱导血小板聚集。这些活性能被过氧化氢酶所抑制，说明T1vf LAO生理学功能主要是通过酶反应产生的过氧化氢( H202)介导的。

基于形象化标签推荐技术的聋儿康复资源系统

针对目前教育、教学资源系统中存在的问题,在以用户为中心的场景设计方法指导下,提出了简单、易学、易用和高效4个层次递进的系统设计开发目标,并重点分析研究了Web2.0的个性化服务:标签和推荐服务。结合这两种服务的优势,在提出和实现形象化标签推荐技术后,阐述了其设计与实现。结合聋儿康复资源系统的设计与开发,详细地介绍了整个系统的功能框架、实现流程以及形象化标签在系统中的运用。最后对全文进行了总结,并对下一步研究工作做出了展望。

语前聋小儿人工耳蜗植入术后的言语康复

An Approach to the Rehabilitation of Prelingually Deaf Children After Cochlear Implantation Zheng Xiujin(Medical Psychology) Directed by Professor Yin WenGang Abstract Objective: To sum up the acquirement rule of speech and language capability which is for the prelingually deaf children after cochlear implantation by listening and language rehabilitation training and to investigate the factors that affect rehabilitation speed. Method: Sixty-four children received a cochlear implant at the age of 2 to 5 years from 2001 to 2005. They begin to be trained under group pattern after switch on 1 month. The whole training program lasted more than 7 months; after that, according to the teacher’s plan the training program was to be continued at home. Result: The period is 108±7.7 days that they can pronounce correctly 50 percent of all of simple-finals and compound-finals, the period is 115.0±7.8 days that they begin auditory repeating, the period is 135.3±10.9 days that they can speech the first specific word independently and the period is 200.3±13.9 days that they can speak 70 words and come into tri-gamut-word and two-word sentence period. The patient that is the group at the age of 2-3 years can take part in normal kindergarten after switch on about 10 months. There are no significant differences in various grades of speech-language development with different age groups and so do with different sex groups. There are significant differences in various grade of speech-language development with various IQ group (P<0.01) and so do with using and not using hearing aids before implantation. Conclusion: From the research we find that the speech and language development sequence is the same level between the prelingually deaf children of 2 to 5 years who received cochlear implant after speech training and normal children and which are stages of uncomplicated sound production, continuous syllabic (babbling), speech sprout, single-word utterances and two-word utterances in proper order. The time is short significantly and the reason is that cognition capability is enhanced along with the increase of age. The intelligence is main factor that affect rehabilitation speed and the speed in the group of high IQ is faster than common IQ. It is not because of the dominance cognition of the senior group that makes the increasing of the rehabilitation, it even makes slowly. The reason of which is that the senior group are exposed the language environment too late to achieve speech and language development. So we should perform an operation and training early. The effectiveness of rehabilitation after cochlear implantation is improved by using hearing aids before implantation. The reason is auditory stimulate can be benefit of to deaf children. The rehabilitation speeds in the children at the age of 2 to 5 years have nothing to do with sex. Key words: cochlear implant; speech therapy; paediatric rehabilitation