461 resultados para Capillary Eletrophoresis

em Chinese Academy of Sciences Institutional Repositories Grid Portal


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1999年,在我国实践5号卫星上完成了两层流体空间实验,实验研究两层不相混合流体的纯Marangoni对流(温度梯度与界面垂直)与热毛细对流(温度梯度方向与流体界面平行).前者存在发生Marangoni对流的最小临界温差值△Tc,低于该值流体系统处于静止状态;后者中只要存在沿界面的温度梯度便会产生热毛细对流.空间实验采用石蜡和氟化液两层流体新体系,实现了平整的液-液交界面,并从卫星上传回上万幅数字图像.通过多幅图像叠加处理得到了定量的流速场.数值模拟计算分析了相应工况时对流流动的速度场,两者的流场结构和速度大小基本一致,实验验证了理论模型.

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In a vertically oscillating circular cylindrical container, singular perturbation theory of two-time scale expansions was developed in inviscid fluids to investigate the motion of single free surface standing wave including the effect of surface tension.

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In the field of fluid mechanics, free surface phenomena is one of the most important physical processes. In the present research work, the surface deformation and surface wave caused by temperature difference of sidewalls in a rectangular cavity have been investigated. The horizontal cross-section of the container is 52 mmx42 mm, and there is a silicon oil layer of height 3.5 mm in the experimental cavity. Temperature difference between the two side walls of the cavity is increased gradually, and the flow on the liquid layer will develop from stable convection to un-stable convection. An optical diagnostic system consisting of a modified Michelson interferometer and image processor has been developed for study of the surface deformation and surface wave of thermal capillary convection. The Fourier transformation method is used to interferometer fringe analysis. The quantitative results of surface deformation and surface wave have been calculated from a serial of the interference fringe patterns.The characters of surface deformation and surface wave have been obtained. They are related with temperature gradient and surface tension. Surface deformation is fluctuant with time, which shows the character of surface wave. The cycle period of the wave is 4.8 s, and the amplitudes are from 0 to 0.55 mu m. The phase of the wave near the cool side of the cavity is opposite and correlative to that near the hot side. The present experiment proves that the surface wave of thermal capillary convection exists on liquid free surface, and it is wrapped in surface deformation.

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An optical diagnostic system consisting of the Michelson interferometer with the image processor has been developed for the study of the kinetics of the thermal capillary convection. The capillary convection, surface deformation, surface wave and the velocity field in a rectangular cavity with different temperature's sidewalls have been investigated by optical interference method and PIV technique. In order to calculate the surface deformation from the interference fringe, Fourier transformation is used to grating analysis. The quantitative results of the surface deformation and surface wave have been calculated from the interference fringe pattern.

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The influence of the indenter shapes and various parameters on the magnitude of the capillary force is studied on the basis of models describing the wet adhesion of indenters and substrates joined by liquid bridges. In the former, we consider several shapes, such as conical, spherical and truncated conical one with a spherical end. In the latter, the effects of the contact angle, the radius of the wetting circle, the volume of the liquid bridge, the environmental humidity, the gap between the indenter and the substrate, the conical angle, the radius of the spherical indenter, the opening angle of the spherical end in the truncated conical indenter are included. The meniscus of the bridge is described using a circular approximation, which is reasonable under some conditions. Different dependences of the capillary force on the indenter shapes and the geometric parameters are observed. The results can be applicable to the micro- and nano-indentation experiments. It shows that the measured hardness is underestimated due to the effect of the capillary force. (c) 2008 Elsevier Ltd. All rights reserved.

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In this paper, we examine a new basic state of long axisymmetric liquid zone, subjected to axial temperature gradients which induce steady viscous flow driven by thermocapillarity. Axial velocity 1/4S-1/2R(B) of liquid zone connects pulling velocity of single crystal. The stability of liquid zone with pulling velocity 1/4S-1/2R(B) to small axisymmetric disturbance is examined The eigenvalue problems on the stability are derived. A special case (eta = 0) is discussed.

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In this paper, applying the direct variational approach of first-order approximation to the capillary instability problem for the eases of rotating liquid column, toroid and films on both sides of cylinder, we have obtained the necessary and sufficient conditions for motion stability of the "cylindrical coreliquid-liquid-cylindrical shell" systems. The results obtained before are found to be special cases of the present investigation. At the same time, we have explained physical essence of rotating instability and settled a few disputes in previous investigations.

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In the present research work, the thermal capillary convection has been investigated and measured by particle image velocimetry (PIV) technique. There is one liquid layer in a rectangular cavity with different temperature’s sidewalls. The cavity is 52mm,42mm,20mm, 4mm in height of the silicon oil liquid layer. A sidewall of the cavity is heated by electro-thermal film, another sidewall is cooled by the semiconductor cooling sheet. The velocity field and the stream lines in cross section in liquid layer have been obtained at different temperature difference. The present experiment demonstrates that the pattern of the convection mainly relates with temperature difference.

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An optical diagnostic system consisting of the Michelson interferometer with the image processor has been developed for studying of the surface wave in the thermal capillary convection in a rectangular cavity. In this paper, the capillary convection, surface deformation and surface wave due to the different temperature between the two sidewalls have been investigated. The cavity is 52mm?42mm in horizontal cross section and 4mm in height. The temperature difference is increased gradually and flow in liquid layer will change from steady convection to unstable convection. The optical interference method measures the surface deformation and the surface wave of the convection. The deformation of the interference fringes, which produced by the meeting of the reflected light from the liquid surface and the reference light has been captured, and the surface deformation appears when the steady convection is developed. The surface deformation is enhanced with the increasing of the temperature difference, and then several knaggy peeks in the interference fringes appear and move from the heated side to the cooled side, it demonstrates that the surface wave is existed. The surface deformation, the wavelength, the frequency, and the wave amplitude of the surface wave have been calculated.

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Models describing wet adhesion between indenters and substrates joined by liquid bridges are investigated. The influences of indenter shapes and various parameters of structures on capillary force are focused. In the former, we consider several shapes, such as conical, spherical and truncated conical indenter with a spherical end. In the latter, the effects of the contact angle, the environmental humidity, the gap between the indenter and the substrate, etc. are included. Different dependences of the capillary force on the indenter shapes and the geometric parameters are observed. Most interesting finding is that applying the present results to micro- and nano-indentation experiments shows the size effect in indentation hardness not produced but underestimated by the effects of capillary force.(4 refs)

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The static and dynamic instabilities of a torsional MEMS/NEMS actuator caused by capillary effects are studied, respectively. An instability number, eta, is defined, and the critical gap distance, g(cr), between the mainplate and the substrate is derived. According to the values of eta and g, the instability criteria of the actuator are presented. The dimensionless motion equation of the MEMS/NEMS torsional actuator is derived when it makes nonlinear oscillation under capillary force. The qualitative analysis of the nonlinear equation is made, and the phase portraits are presented on the phase plane. In addition, the bifurcation phenomena in the system are also analyzed. (C) 2008 Elsevier Inc. All rights reserved.

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A new dual simultaneous detector was developed for capillary electrophoresis microchip. Confocal laser-induced fluorescence (LIF) and moveable contactless conductivity detection (MCCD) were combined together for the first time. The two detection systems shared a common detection cell and could respond simultaneously. They were mutually independent and advantageous in analyses of mixtures containing organic and inorganic ions. The confocal LIF had high sensitivity and the MCCD could move along the separation channel and detect in different positions of the channel. The detection conditions of the dual detector were optimized. Rhodamine B was used to evaluate the performance of the dual detector. The limit of detection of the confocal LIF was < 5 nM, and that of the MCCD was 0.1 mu M. The dual detector had highly sensitivity and could offer response easily, rapidly and simultaneously. 

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The influence of contact angle and tube radius on the capillary-driven flow for circular cylindrical tubes is studied systematically by microgravity experiments using the drop tower. Experimental results show that the velocity of the capillary flow decreases monotonically with an increase in the contact angle. However, the time-evolution of the velocity of the capillary flow is different for different sized tubes. At the beginning of the microgravity period, the capillary flow in a thinner tube moves faster than that in a thicker tube, and then the latter overtakes the former. Therefore, there is an intersection between the curves of meniscus velocity vs microgravity time for two differently sized tubes. In addition, for two given sized tubes this intersection is delayed when the contact angle increases. The experimental results are analyzed theoretically and also supported by numerical computations.