Studies of the intermolecular DNA triplexes of C+center dot GC and T center dot AT triplets by electrospray ionization Fourier-transform ion cyclotron resonance mass spectrometry

Formation and stabilities of four 14-mer intermolecular DNA triplexes, consisting of third strands with repeating sequence CTCT, CCTT, CTT, or TTT, were studied by electrospray ionization Fourier-transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) in the gas phase. The gas-phase stabilities of the triplexes were compared with their CD spectra and melting behaviors in solution, and parallel correlation between two phases were obtained. In the presence of 20 mm NH4+ (pH 5.5), the formation of the TTT triplex was not detected in both solution and the gas phase.

米托蒽醌与双链及三链核酸相互作用的电喷雾质谱研究

The interaction of mitoxantrone (MXT) with duplex and triplex DNA, contain repeating sequence CTCT, CCTT and CTT were studied by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS). The 1:3 specific complexes of mitoxantrone and duplex DNA and 1:2 specific complexes of mitoxantrone and triplex DNA were observed. The results show that mitoxantrone has no remarkable sequence selectivity, however it has distinct structure selectivity, and destabilization the triplex.

社会发展与中国人人格变迁——控制测验理论与工具的变异后不同时期的中国人人格差异研究

In non-western society，researches on social development and personality change focused on economic development and social modernization. The present study is aimed at exploring the relationship between the social transformation and personality changes of Chinese people by using so-called indigenous personality measurement of CPAI (Chinese Personality Assessment Inventory). Meanwhile, the influence of CPAI measurement itself and measurement theory were also taken into consideration. In study 1, two sets of CPAI data collected in a 10 year interval were analyzed. At the same time, the CPAI-2 data was analyzed in terms of modernization level of various cities from which the data were collected. However, this study didn’t consider the importance of “equivalence” of the measurement, CPAI. In study 2, we detected DIF (Differential item functioning) across the different period groups to confirm if CPAI was equal to people in different period. In this process, both CTT and IRT method were used. The outcome reminded us that there were some DIF items. In study 3, to make sure that the personality measurement is fair to people in different period, we only saved those items whose DIF effect size lower than 0.01, and used IRT method to estimate test-taker’s personality. Then, cohort analysis was used to explore the pattern of personality change of Chinese people. In study 4, we factor-analyzed the DIF items to find the relation between social transformation and the latent personality variable which were composed of DIF items. From these 4 studies, we could got the following conclusions: (1) The CPAI 22 traits could be divided into two categories, with the changing of age, period and cohort, type I traits didn’t change, they were Logical vs Affective Orientation, Enterprise, Responsibility, Inferiority vs Self-Acceptance, Optimism vs Pessimism, Face, Family, Defensive, Graciousness vs Meanness; While with the changing of age, period and cohort, type II traits changed, they were Leadership, Self vs. Social Orientation, Veraciousness vs Slickness, Traditionalism vs Modernity, Harmony, Renqing, Meticulousness, Extraversion vs Introversion, Emotionality, Practical Mindedness, Internal vs External Locus of Control, Thrift vs Extravagance, Discipline. Meanwhile DIF items measured 5 psychologycial characteristics which changed greatly with the changing of age, period and cohort, they were Life attitude of Cynicism-realism, Psychological maladjustment, Coping style of Waiyuanneifang, Self-efficacy, the value of Individualism. (2) In sum, Chinese people in 1992 were more traditional than those in 2001, and with the 10-year of rapid development, according to the market economy’s needs, Chinese people became more individualism. (3) The DIF method of CTT and IRT were comparable. But, in generally, IRT method was more accurate and valid in detecting DIF as were as estimating personality. (4) The DIF outcomes showed that CPAI had good item validity. Meanwhile, it’s possible to develop a subscale by using CPAI items to assess some psychological characteristics. In this current study, according to their stability and variability, we could divided personality traits and psychological characteristics into 3 categories, and the outcome supported the hypothesis of “Six Factor Model”, these foundings were of some theoretic meanings. Meanwhile, as the relation between social development and personality change being explored, it certain help Chinese people cope with the rapid changing society. In this study, we also found that it’s possible to develop a subscale by using CPAI items to assess obverse personality traits and it had some practical use. Furthermore, the use of different measurement theory and cohort analysis embodied some innovation in methodology.