19 resultados para AK26A-2589
em Chinese Academy of Sciences Institutional Repositories Grid Portal
Resumo:
比较了掺钕光纤和掺镱光纤对1064nm光放大的特点.基于速率方程和传输方程,数值分析了在915nm泵浦下双程掺镱光纤放大器的增益特征,并且和传统的单程放大进行了比较.分析了信号光和泵浦光,以及粒子数沿着光纤方向的分布特点.最后对双程掺镱光纤放大器进行了优化.
Resumo:
A novel Littman-Metcalf external cavity laser diode array with two feedback mirrors is introduced. The line-width broadening effect caused by smile can be reduced by the novel external cavity. At the drive current of 16A, the line-width is narrowed to 0.1nm from free-running width of 1.6nm with output efficiency of 84%.
Resumo:
中国科学院昆明动物研究所始建于1959年. 历经40余年的艰苦努力, 现已发展成为具有一定规模和特色、多学科相互交叉渗透的综合性动物学研究机构. 1999~2000年, 在中国科学院知识创新工程建设的推动下, 昆明动物研究所在科研目标凝练和学科建设、创新机制转换、队伍建设和人才培养, 科研成果转化, 创新文化与园区建设等方面都取得了长足的进展。
Resumo:
GaInAsP-InP microsquare resonators with InP pedestals are fabricated by two-step chemical etching, and obvious mode peaks are observed in the photoluminescence spectra of the resonators. The mode Q-factors about 500 are obtained for a microsquare resonator with the side length of 7 mu m. The experimental mode interval is in agreement with that predicted by the light ray method based on the cavity length, instead of that of the whispering-gallery (WG)-like modes, which has mode interval twice of that determined by the cavity length. The finite-difference time-domain simulation shows that a little asymmetry may greatly reduce the difference of the Q-factors between the WG-like modes and the other modes.
Resumo:
东北黑土区是我国重要的商品粮生产基地,在保证我国的粮食安全中发挥着重要作用。随着生产的发展,多种农用化学品尤其是大量杀虫剂和除草剂进入黑土农田环境,在预防作物病虫害及减少人工劳动强度的同时,这些化学品在环境中会发生一系列复杂的物理、化学和生物化学反应,使黑土面临着环境安全危机。因此,研究农用化学品的土壤生态过程与黑土农田生态系统环境安全有关的科学问题显得尤为重要。 本文以东北地区广泛使用的除草剂乙草胺为代表,在推荐施用量条件下,通过室内恒温(25℃)避光培养试验,研究了乙草胺在黑土中的主要生态过程(降解、吸附)及在土壤中的残留时间特征,探讨了影响乙草胺生态过程行为的主要因素,同时利用磷脂脂肪酸(Phospholipid Fatty Acids,PLFAs)为标识物研究了土壤微生物对外源污染物的的响应。 研究结果表明:吸附是乙草胺在土壤中主要去向之一,高有机质黑土对乙草胺的吸附能力很强。微生物降解是乙草胺消失的另一个重要要因素,适当的水分有益于土壤中微生物生长,从而促进土壤中乙草胺降解,其中,细菌比真菌具有更强的降解乙草胺的能力;随着乙草胺的施药量增加,乙草胺总降解率下降。在乙草胺施入及降解过程中,土壤微生物群落不断发生变化,真菌PLFAs、细菌PLFAs;G+菌PLFAs、G-菌PLFAs数量及比例受到乙草胺施入的显著影响,细菌比真菌的变化更为显著。原位土壤中,乙草胺在表层土壤(0-10cm和10-20cm)微生物降解作用显著;而降水是促进乙草胺向深层次迁移的主要因素。 通过对乙草胺的主要生态过程进行研究,对深入探讨乙草胺生态环境安全性及合理使用除草剂有着重要的指导作用。
Resumo:
赤霉素是一种高效能的广谱植物生长调节剂,为五大植物激素之一,具有重要的生物学功能。目前利用赤霉素突变体研究生物合成途径和信号转导已经成为热点。 GA 20-氧化酶是GA生物合成中的一类关键酶,它位于GA合成途径的中心位置。本研究根据烟草(Nicotiana tabacum)GA 20-氧化酶基因序列,设计2对分别含有特定酶切位点的特异引物,以烟草基因组DNA为模板,扩增目的基因(约250 bp)片段。将正、反向目的片段分别插入中间载体的内含子两侧,再经BamH I和Sac I双酶切回收约700 bp的目的片段,插入到双元载体质粒p2355中,成功构建了含GA 20-氧化酶基因片段反向重复序列的植物表达载体p23700。分别将p2355质粒和p23700质粒导入根癌农杆菌(Agrobacterium tumefaciens)EHA105中并转化烟草叶片细胞,经卡那霉素选择培养,PCR及GUS组织染色鉴定,获得转基因烟草植株。以EHA105-p2355转化的烟草,获得41株转基因植株,均没有矮化表型;而以EHA105-p23700转化的烟草,获得转基因植株14株,其中具有矮化表型的烟草10株,表明反向重复序列转录产物能形成发夹RNA(hpRNA),产生小分子干扰RNA(small interferring RNA,简称siRNA),干扰目的基因的表达。 赤霉素含量测定表明矮化植株中赤霉素合成途径的最终产物GA3总含量明显低于野生型烟草植株。荧光定量PCR结果表明,矮化转基因烟草的GA 20-氧化酶基因表达量受到明显抑制,表达量明显低于野生型对照。同时对上游内根-贝壳杉合成酶(Ent-kaurene synthase,KS)基因,下游的GA-3β羟化酶基因进行了RT-PCR分析,结果显示上游基因的表达没有规律性变化,而下游基因表达量亦降低。上述结果表明,GA 20-氧化酶基因的表达被有效地干扰了,表达受到抑制,从而影响植株体内GA3的合成,影响植株的生长发育,导致植株矮化。并推测,GA 20-氧化酶基因受到抑制,可能影响下游基因的表达。并且通过干旱胁迫测试,发现矮化植株相对于野生型植株及不含干扰片段的转基因植株,对干旱的耐受力有了很大的提高,具有更强的耐受力。 研究结果为进一步进行相关研究奠定基础。 Gibberellin(GA) is an efficient plant growth regulator. As one of five major plant hormones, it plays an important biological function. Using GA mutant for investigating biosynthetic pathways and signal transduction has become high lights. GA 20-oxidase is a crucial enzyme involved in gibberellin biosynthesis. According to tobacco (Nicotiana tabacum) GA 20-oxidase enzyme gene sequence and based on binary vector p2355, we constructed a plant expression vector p23700, which habors an inverted repeat DNA fragment of GA 20-oxidase gene drivered by Cauliflower mosaic virus promtor (CaMV 35Sp). Binary plasmid p2355 had no inverted repeat DNA fragment of GA 20-oxidase gene. The vector p2355 and p23700 were introduced into Agrobacterium tumefaciens EHA105 and tobacco leaf transformation was conducted. After selected by kanamycin and characterized by PCR and GUS hischemical reaction, transsgenic plants were obtained. Fourtheen transgenic plants, which were transformed by EHA105-p23700, were obtained. Among them, 10 were dwarf mutants. However, 41 transgenic plants with the same normal phenotype as wild type,which were transformed by EHA105-p2355, were obtained. Analysis of Gibberellin contents showed that it was lower in dwarf mutants than in normal phenotype plants. Moreover, comparing to normal phenotype plants including wild type and transgenic plants with no interference fragment, the drought tolerance of dwarf plants have greatly increased. And their proline content increased obviously after drought test. Fluorescence quantitative real time PCR (RT-PCR) showed that GA 20-oxidase gene expression was significantly inhibited in dwarf transgenic tobacco. Meanwhile, the expression of the upstream gene ent-kaurene synthase (KS) gene and downstream gene GA-3β hydroxylase gene was also detected by RT-PCR. The results presented that KS gene expression had no regular change while GA-3β hydroxylase gene expression reduced. It implied that inhibiting GA 20-oxidase gene probably reduce the expression of downstream genes. The results showed that the transcriptional products of the foreign inverted repeat fragment can form hairpin RNA (hpRNA) to induce RNAi. It presented that GA 20-oxidase gene expression was effectively interfered, resulting in reducing GA3 synthesis and inhibiting plant growth and development, then dwarf plants were produced. However, the dwarf plants had higher tolerance of drought.
Resumo:
在ECPSSR理论的基础上,利用OBKN近似描述电子俘获过程,得到了包括电子俘获过程贡献的ECPSSR理论,编写了相应的计算程序。采用该程序计算了不同电荷态离子与多种靶原子碰撞的电子俘获截面和相应的X射线产生截面,将计算得到的包含电子俘获过程贡献的X射线产生截面与实验结果进行了比较。对于具有满K壳层的入射离子碰撞,X射线产生截面与入射离子电荷态基本无关;对于以直接电离为主导的碰撞过程,计算得到的X射线产生截面与实验数据符合得很好;对于全裸和单K空穴入射离子的碰撞,计算高估了X射线产生截面。
Resumo:
基于中国1980-2000年气候数据及两期土地利用/土地覆盖(LUCC)数据,利用Holdridge植被生态分区模型、重心模型及土地利用程度模型,分析气候变化及人类活动对中国植被覆盖及土地利用的影响程度及变化趋势。1980-2000年间,中国大部分地区温度升高,降水增多。气候变化不仅影响了中国植被群落分区,更进一步影响了植被群落的生长状况;东北、华北、内蒙古高原等区域未利用土地型向草地和灌木生态类型转换,草地和灌木型生态类型向林地和耕地型转换;又因为1980-2000年间中国经济的发展,东部沿海区域城市乡村建设用地及交通用地的增多,使土地利用类型南农业耕地型向建设用地型发展,导致土地利用程度指数的升高。气候变化及经济发展的双重作用,导致中国土地利用程度重心20年来向东北方向移动了54km,东西方向土地利用程度偏移强度,气候占81%,人类活动占19%,南北方向土地利用程度偏移强度,气候占85%,人类活动占15%。
Resumo:
一种温和解聚聚对苯二甲酸丙二醇酯的方法是以甲醇为解聚剂,将聚对苯二甲酸丙二醇酯与甲醇的重量比为1∶1-20,解聚反应温度介于150-300℃之间,压力为甲醇自生成压力,解聚时间为0.1-10.0小时。聚对苯二甲酸丙二醇酯的废料解聚为对苯二甲酸二甲酯(DMT)和1,3-丙二醇(PDO)。本发明具有工艺流程简单、易于操作、定向生成单体产物DMT和PDO的优点。