84 resultados para 589
em Chinese Academy of Sciences Institutional Repositories Grid Portal
Resumo:
Pressure wave refrigerators (PWR) refrigerate the gas through periodical expansion waves. Due to its simple structure and robustness, PWR may have many potential applications if the efficiency becomes competitive with existing alternative devices. In order to improve the efficiency, the characteristics of wave propagation in a PWR are studied by experiment, numerical simulation and theoretical analysis. Based on the experimental results and numerical simulation, a simplified model is suggested, which includes the assumptions of flux-equilibrium and conservation of the free energy. This allows the independent analysis of the operation parameters and design specifics. Furthermore, the optimum operation condition can be deduced. Some considerations to improve the PWR efficiency are also given.
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By means of a surface plastic deformation method a nanocrystalline (NC) intermetallic compound was in situ synthesized on the surface layer of bulk zirconium (Zr). Hardened steel shots (composition: 1.0C, 1.5Cr, base Fe in wt.%) were used to conduct repetitive and multidirectional peening on the surface layer of Zr. The microstructure evolution of the surface layer was investigated by X-ray diffraction and scanning and transmission electron microscopy observations. The NC intermetallic layer of about 25 gm thick was observed and confirmed by concentration profiles of Zr, Fe and Cr, and was found to consist of the Fe100-xCrx compound with an average grain size of 22 nm. The NC surface layer exhibited an extremely high average hardness of 10.2 GPa. The Zr base immediately next to the compound/Zr interface has a grain size of similar to 250 nm, and a hardness of similar to 3.4 GPa. The Fe100-xCrx layer was found to securely adhere to the Zr base. (c) 2007 Elsevier B.V All rights reserved.
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<正> 一、引言 动导数对于飞行器的动态品质分析和控制系统的设计是必不可少的参数,它直接影响飞行器的动态特性。因此,国外自七十年代以来,无论在风洞实验、飞行试验,还是分析、计算等方面都作了很多工作,取得了较大的进展。 动导数计算说到底主要是非定常气动力计算问题,已有的线化理论不能满足需要,对考虑非线性效应的动导数计算方法的需求则日显迫切。二次理论是计算非线性效应的一种方法,它是由第二次叠代或物面偏角参数的二次展开法构成,主要非线性效应由二
Resumo:
本文在提出广义Gauss定理的思路下,提出了广义Stokes定理和一个推广Virial定理的新方法,后者应用于天体磁流体力学和引力平衡问题时得到的结果有:(1)气体具有运动时的平衡系统的判据,(2)磁场对气团形态的影响。应用于实验室等离子体平衡问题时,其结果有:(1)包围在气体中孤立磁场的特性,(2)发现内包无力场必须外包一有力场,(3)无力场的形态,应用于Tokmak等离子体环时的结果有:(1)环的胖瘦对环表面磁压的影响,(2)两个外加磁场分量分别和气压、环的胖瘦,截面形态、环电流分布和逆磁或顺磁的平衡关系,搞清楚了外加磁场约束等离子体总体平衡的物理机制。
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本文提出了分析研究岩石杆中应力波衰减机制(初始微裂纹、节理、剪胀等因素的影响)的一种方法,并相应提出了减少波形振荡,提高试验精度的措施。利用这种方法,对这些因素引起应力波衰减的特点进行了初步探讨。此外还提出了在波传播时幅值无衰减的情况下,从一根杆试验中测得的应变波形,求得包括卸载在内的完整的动态滞迴应力应变关系的方法,这个方法比SHPB法更方便。实验结果说明,完整致密的石灰岩只有在剪张点以下方可当作弹性材料处理。
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The effects of stochastic extension on the statistical evolution of the ideal microcrack system are discussed. First, a general theoretical formulation and an expression for the transition probability of extension process are presented, then the features of evolution in stochastic model are demonstrated by several numerical results and compared with that in deterministic model.
Resumo:
Poly(dimethylsiloxane) (PDMS) is usually considered as a dielectric material and the PDMS microchannel wall can be treated as an electrically insulated boundary in an applied electric field. However, in certain layouts of microfluidic networks, electrical leakage through the PDMS microfluidic channel walls may not be negligible, which must be carefully considered in the microfluidic circuit design. In this paper, we report on the experimental characterization of the electrical leakage current through PDMS microfluidic channel walls of different configurations. Our numerical and experimental studies indicate that for tens of microns thick PDMS channel walls, electrical leakage through the PDMS wall could significantly alter the electrical field in the main channel. We further show that we can use the electrical leakage through the PDMS microfluidic channel wall to control the electrolyte flow inside the microfluidic channel and manipulate the particle motion inside the microfluidic channel. More specifically, we can trap individual particles at different locations inside the microfluidic channel by balancing the electroosmotic flow and the electrophoretic migration of the particle.
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Determination of the energy range is an important precondition of focus calibration using alignment procedure (FOCAL) test. A new method to determine the energy range of FOCAL off-lined is presented in this paper. Independent of the lithographic tool, the method is time-saving and effective. The influences of some process factors, e.g. resist thickness, post exposure bake (PEB) temperature, PEB time and development time, on the energy range of FOCAL are analyzed.
Resumo:
The paper comprehensively analyzes the distortions of a circular wedge prism with 400 mm diameter in a scanner by method of optical-mechanical-thermal integrating analysis. The structure and intensity of the prism assembly is verified and checked, and the surface deformations of the prism under gravity load, as well as the thermo-elastic distortions of the prism, are analyzed in detail and evaluated, which is finally contrasted with the measured values of Zygo Mark interferometer. The results show: the maximal distortion of the prism assembly is 10 nm magnitude and the maximal stress is 0.441 Mpa, which has much tolerance to the precision requirement of structure and the admissible stress of material; the influence of heat effect on the surface deformations of prism is proved to be far greater than the influence of gravity load, so some strict temperature-controlled measures are to be considered when the scanner is used. (c) 2006 Elsevier GmbH. All rights reserved.
Resumo:
abstract {Proton induced X-ray emission (PIXE) technique is an effective method for the chemical composition analysis of ancient glass samples without destruction. Chemical composition of the ancient glass samples dated from the Warring States Period (770-476 B.C.) to the Six Dynasties Period (220-589 A.D.), which were unearthed in Sichuan area, was quantitatively determined by the PIXE technique. The results show that the glass Bi (disc) and the glass eye beads of the Warring States Period all belong to the PbO-BaO-SiO2 system. According to the composition and shape, we infer that these glass Bi and eye beads were made in China. Whereas, the chemical compositions of the glass ear pendants and beads of the Six Dynasties Period are varied, including K2O-CaO-SiO2, K2O-SiO2 and other glass systems. Based on the obtained results and those from literatures, some questions related to the technical propagation of the ancient Chinese glass are discussed.}
Resumo:
一、棕色固氮菌突变种DJ35固氮酶钼铁蛋白的纯化、体外重组及结晶研究 棕色固氮菌(Azotobacter vinelandii Lipmann)突变种DJ35的菌体破碎后,所得的未经加热的粗提物经DEAE Cellulose 52柱层析后得到部分纯的钼铁蛋白(ΔnifE Av1)和铁蛋白(Av2)。部分纯的ΔnifE Av1再经Sephacryl S-300和Q-Sepharose Fast Flow柱层析进一步纯化,便首次得到SDS凝胶电泳检测为基本纯的ΔnifE Av1。SDS-PAGE及Western blotting的结果表明,ΔnifE Av1具有与野生型棕色固氮菌钼铁蛋白(OP Av1)相同的亚基种类和组成(α2β2)。质子还原活性测定表明,在与Av2进行活性互补时ΔnifE Av1不具有明显的质子还原活性,而与从OP Av1抽提出的FeMoco抽提液保温后便可与Av2实现活性互补。这表明,ΔnifE Av1是一种缺失FeMoco的钼铁蛋白。 将ΔnifE Av1用过量的邻菲啰啉(o-phenanthroline)厌氧处理并经Sephadex G-25柱层析分离后,便得到部分丢失Fe的ΔnifE Av1©。在同时存在Av2和MgATP发生系统的条件下,ΔnifE Av1©, 而不是处理前的ΔnifE Av1,可为由KMnO4或Na2CrO4、高柠檬酸铁、Na2S、Na2S2O4 和二硫苏糖醇组成的含Mn或含Cr重组液(RS-Mn或RS-Cr)显著激活,但在缺少MgATP或Av2的条件下,RS-Mn和RS-Cr则不能激活ΔnifE Av1©。这就表明,RS-Mn和RS-Cr对ΔnifE Av1©的激活都需要邻菲啰啉的预处理及Av2和MgATP的同时存在。从分别缺失nifZ和nifB点突变的固氮菌突变种DJ194和UW45中纯化得到的钼铁蛋白,ΔnifZ Av1和NifB- Av1,经邻菲啰啉厌氧处理并经Sephadex G-25柱层析分离后,也分别得到部分丢失Fe的ΔnifZ Av1©和NifB- Av1©。与ΔnifE Av1©一样,这两种蛋白在Av2和MgATP同时存在时也可被RS-Cr和含Mo重组液(RS-Mo)明显激活。 为获得可供X-射线衍射的ΔnifE Av1的大单晶,对组成蛋白质沉淀剂的各种化合物的种类和浓度、缓冲液的pH值、结晶方法及蛋白样品的批次、浓度等结晶条件进行了大量优化研究。首次获得了ΔnifE Av1的深棕色短斜四棱柱晶体,并对其蛋白组成进行了鉴定。 二、铬铁蛋白中残存的棕色固氮菌细菌铁蛋白的晶体生长及鉴定 从无钼、无氨而含铬的固氮培养基中生长的棕色固氮菌突变种UW3中纯化得到了部分纯的CrFe蛋白。在试图培养CrFe蛋白大晶体时发现,棕色晶体和砖红色晶体可同时或单独出现。SDS-和厌氧天然-PAGE皆表明,棕色晶体主要由与Av1类似大小的亚基(~60 kD)组成,而砖红色晶体则主要由~20 kD亚基组成。Western-blotting表明只有~60 kD亚基可与OP Av1的抗体发生反应,而~20 kD亚基则无这种反应。在部分纯的CrFe蛋白中,~20 kD的蛋白含量远低于~60 kD蛋白的含量,表明由这种小亚基组成的蛋白只是CrFe蛋白中的一种污染蛋白。用3,5-二氨基苯甲酸染色的天然电泳表明,形成砖红色和棕色晶体的蛋白是迁移率不同的两种含铁蛋白。质谱分析表明,该晶体蛋白为棕色固氮菌的细菌铁蛋白(AvBF)。分辨率为2.34 Å的X-射线衍射结果也表明,砖红色晶体属于H3空间群,晶胞参数为a = 124.965 Å, b= 124.965 Å 和 c = 287.406 Å。首次完成的结构解析也表明,这种砖红色晶体确为24聚体的AvBF。 关键词:棕色固氮菌突变种DJ35和UW3; ΔnifE Av1; 铬铁蛋白; 细菌铁蛋白; 纯化和特性; 体外激活组装; 晶体生长及组成鉴定
Resumo:
白沙蒿(Artemisia sphaerocephala Krasch.)是中国西北部沙漠的流动及固定沙丘上广泛分布的优势种灌木。瘦果小而轻;借助显微镜和扫描电镜对瘦果的形态结构进行了观察,其种子的种皮与果皮愈合,果皮外层为很厚的粘液层,其粘液层在遇湿后迅速吸水膨胀,其重量增至原来的589倍。在自然生境中,粘液物质将沙粒粘附于瘦果的周围,使瘦果的重量发生变化。瘦果能长时间在水上漂浮,粘液物质有助于种子的萌发和苗的发育。白沙蒿产生三种不同颜色的瘦果,其种子具有不同的萌发速率但最终达到相同的萌发率。瘦果的粘液物质对白沙蒿种子的传播与萌发具有重要的生态意义。白沙蒿种子为需光种子,种子在光下萌发而在暗中受到抑制。种子萌发的适宜温度为250 C,在100 C和300 C萌发速率和萌发率都很低,萌发在50 C受到抑制。种子在沙中被埋越深,其萌发速率和萌发率就越低。在沙平面下2厘米或更深层沙土下出苗率为零。但是,当将种子上层沙土移走,只保留0.5厘米沙土覆盖种子,这些种子的萌发率达到原先就位于沙土下0.5厘米的种子的萌发率,但是后者的萌发速率较高。土壤水分含量越高,从1. 7%到14. 7%,其萌发就越快。土壤水分含量从19.4%起,种子的萌发受到了延迟,苗的发育受到了抑制。 梭梭(Haloxylon ammodendron)是我国西北荒漠中分布的多年生植物,属中亚荒漠植被成分。其种子萌发的最适温度为loo C,亚适宜温度为15-20 0C。从200C起,温度越高,萌发比率越低。种子无论在光下和暗中都能萌发,萌发率无显著性差异。浓度低于0.2 molL的NaCl溶液对萌发的影响不大;但从0.8 mol/L起,萌发率随着浓度增高而降低,直至为零。将在盐溶液中处理9d的种子转移至蒸馏水后,原来较高浓度下的种子具有较高的萌发恢复率。不同浓度下的种子的萌发率和萌发恢复率要比蒸馏水中的低,表明NaCl处理后的部分种子永久地失去萌发力。梭梭的种子为短命种子,在自然状态下,种子的含水量为8.5%,寿命约为10个月。将梭梭种子含水量降至2. 5%和1.4%,其耐贮藏力增强。经过500 C下5天和10天的人工加速老化后,超干种子表现出较强的抗老化能力。与对照相比,超干种子萌发率、根长及活力指数等均显著提高。较低的电导率和较高的脱氢酶活性,说明种子在超干处理后表现出了膜系统的完整性和较高的种子生活力。超干处理既能显著提高梭梭种子的耐贮性,也能有效保持种质的稳定性,是梭梭种质资源保护的重要途径之一。 沙柳(Salix psammophila)是毛乌素沙地流动沙丘和固定沙丘上广泛分布的重要固沙植物。沙柳叶片的光合速率呈现出双峰型的日动态变化,在午后14点出现光合午休现象。蒸腾速率呈现出单峰型的日动态变化,蒸腾在午后15点达到最大。水分利用效率在早晨9点后保持在2.5 μmol C02.mmol-l H20左右。叶片水分亏缺也表现出规律性的变化。从早晨开始,叶片水分亏缺逐渐增强,在18时达到最大值。比较生长季节的不同时期环境因子的变化情况表明,春末的光照强度和气温比夏末高,但土壤水分含量则相反。沙柳在春末的光合速率、蒸腾速率分别是夏末的79%和72.4%,表明水分亏缺是影响沙柳生长发育的主要限制因素。