Aggregation behavior of amphiphilic D-pi-A molecules bearing recognition group

Aggregation behavior of two amphiphilic D-pi -A molecules bearing barbituric acid as both recogniton group and electron-drawing substituent, 5-(4-dodecyl oxybenzylidene)-(1H, 3H)-2,4,6-pyrimidine trione (PB12) and 5-(4-N,N-didodecyl aminobenzylidene)-(1H,3H)-2,4,6-pyrimidine trione (AB(12)) was studied by UV-visible, fluorescence, and surface voltaic spectroscopies (SPS). The experimental results indicate that PB12 tends to form J-aggregate and AB(12) tends to form H-aggregate under increasing concentration. An intramolecular twisted charge transfer (TICT) emission around 500 nm is observed when J-aggregate is formed between PB12 molecules, and an excimer emission around 600 nm is observed when H-aggregate is formed between AB(12) molecules.

Acid-stable amperometric soybean peroxidase biosensor based on a self-gelatinizable grafting copolymer of polyvinyl alcohol and 4-vinylpyridine

A peroxidase was extracted from Chinese soybean seed coat, and its thermostability and acid-stability were characterized. This peroxidase was immobilized into a self-gelatinizable grafting copolymer of polyvinyl alcohol with 4-vinylpyridine(PVA-g-PVP) to construct an acid-stable hydrogen peroxide biosensor. The effect of pH was studied for optimum analytical performances by amperometric and spectro-photometric methods, also the K-m(app) and the stability of the soybean peroxidase-based biosensor are discussed. At pH 3.0, the soybean peroxidase maintained its bioactivity and the enzyme electrode had a linear range from 0.01 to 6.2 mM with a detection limit of 1.0 x 10(-7) M. In addition, the main characteristics of different hydrogen peroxide sensors were compared.

Synthesis and X-ray crystal structure of [(eta(5)-C5H5)Sm(mu-OC20H20N2O)](2)(mu-THF)(THF)(2): Mono(cyclopentadienyl)lanthanide complex bearing a C-2-symmetric tetradentate Schiff-base ligand

Cp2SmCl(THF) reacts with 0.5 equivalent disodium salts of trans-(+/-)-N,N'-bis(salicylidene)-1,2-cyclohexanediamine give the title complex [(eta(5)-C5H5)Sm(mu-OC20H20N2O)](2)(mu-THF)(THF)(2) (1). X-ray crystal determination shows that the molecule is a dimer, in which two (eta(5)C(5)H(5))Sm(mu-OC20H20N2O) units are connected via a THF oxygen and two bridging oxygen atoms of Schiff base ligands. The average Sm-C distance is 2.78(7) Angstrom, while those of Sm-O (bridging THF oxygen) and Schiff base oxygens are 2.79(3) and 2.43(4) Angstrom; respectively. (C) 1998 Elsevier Science Ltd. All rights reserved.

高效毛细管电泳柱端安培检测异丙嗪

本文用高效毛细管区带电泳柱端安培检测了抗组胺药物异丙嗪（ＰＭＺ）。讨论了缓冲溶液浓度，ｐＨ值对柱效的影响，为提高检测的重现性考察了电极的电化学处理条件。在优化的实验条件下，异丙嗪的线性范围为１×１０－７～１×１０－４ｍｏｌ／Ｌ，检测下限为３．５×１０－８ｍｏｌ／Ｌ，分离并检测了尿样中的１×１０－７ｍｏｌ／Ｌ的异丙嗪。

Laser light-scattering study of novel thermoplastics .1. Phenolphthalein poly(aryl ether ketone)

Five different molecular weight phenolphthalein poly(aryl ether ketone) (PEK-C) fractions in CHCl3 were studied by static and dynamic laser light scattering(LLS). The dynamic LLS revealed that the PEK-C samples contain some large polymer clusters. These large clusters can be removed by filtering the solution with a 0.1-mu m filter. We found that the persistence length of PEK-C in CHCl3 at 25 degrees C is similar to 2 nm and the Flory characteristic ratio, C-infinity is similar to 25. Our results showed that [R(g)(2)](1/2)(z) = (3.50+/-0.20) x 10(-2)M(w)(0.54+/-0.01) and [D] = (2.37+/-0.05) x 10(-4)M(w)(-0.55+/-0.01), with [R(g)(2)](1/2)(z), M(w), and [D] being the z-average radius of gyration, the weight-average molecular weight, and the z-average translational diffusion coefficient, respectively. A combination of static and dynamic LLS results enabled us to determine D = (2.20+/-0.10) x 10(-4)M(-0.555+/-0.015), where D and M correspond to monodisperse species. Using this calibration between D and M,we have determined molecular weight distributions of five PEK-C fractions from their corresponding translational diffusion coefficient distribution.

聚环氧氯丙烷聚氨酯／聚苯乙烯IPN的玻璃化转变行为

用ＤＳＣ和动态弹粘谱（ＤＭＳ）研究了聚环氧氯丙烷聚氨酯／聚苯乙烯互穿聚合物网络［ＰＵ（ＰＥＣＨ）／ＰＳＩＰＮ］的玻璃化转变行为发现，当ＩＰＮ中聚苯乙烯（ＰＳ）含量大于２０％（质量分数）时，ＩＰＮ是不相容体系，有两个玻璃化转变ＰＳ交链度的增加，对应于ＰＵ的玻璃化转变（ＴｇⅠ）不变，对应于ＰＳ的玻璃化转变（ＴｇⅡ）向高温方向移动，转变越来越不明显，加入氨酯反应催化剂时，Ｔｇ明显内移，ＴｇⅡ内移超过２０℃，相容性增加。

LIQUID-CHROMATOGRAPHY AMPEROMETRIC DETECTION OF CARBOXYLIC-ACIDS AND PHENOLIC-ACIDS WITH A COPPER-BASED CHEMICALLY-MODIFIED ELECTRODE

A copper-based chemically-modified electrode has been constructed and characterized by various experimental parameters in flow-through amperometric detection of carboxylic acids and phenolic acids. Novel hydrodynamic voltamperograms were first obtained in flow-through amperometric detection with the Cu-based CME and subsequently negative and positive peaks were observed in a single chromatogram. This unique and flexible potential dependence could be of great benefit in chromatographic speciation and quantification. These observations suggest that the detector response was governed by the complexation reaction of copper ions with the solutes.

The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis

Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved.

西菲律宾海有机悬浮颗粒分布变化及其光衰减研究

论文以2004年在西菲律宾海获取的光学剖面资料为基础，首次将光学衰减系数作为悬浮有机颗粒的替代指标，对西菲律宾海上层水体悬浮颗粒及颗粒有机碳的时空分布和变化进行了研究。结果表明，光衰减系数cp同颗粒有机碳浓度具有显著的线性相关关系，这对于海洋初级生产力估算和碳循环研究具有重要意义。断面WE和SN上的浮游植物现存量与颗粒有机碳现存量变化趋势完全一致。上层水体中的颗粒有机碳主要来源于有机碎屑颗粒。西菲律宾海混合层的昼夜变化对于表层水体光学变异以及颗粒输出作用具有重要影响。表层水温、光衰减系数cp和混合水深都与海面光合有效辐射同相变化。表层水体中的颗粒有机碳现存量同混合水深同步变化，受混合层控制作用显著；而对于深层水体，推测主要受生物垂向迁移和颗粒聚集沉降的影响。 根据表层水体中微微型自养颗粒的计数分析结果，原绿球藻的丰度最高，其次为聚球藻，自养真核细胞的丰度最低。首次估算了三种微微型颗粒的散射系数，并与光衰减系数cp做了对比分析。研究表明，菲律宾海三种微微型颗粒的散射作用仅占光衰减系数cp的5%，其中原绿球藻、自养真核细胞和聚球藻分别占2.17%、2.85%和0.33%。根据微微型颗粒的散射特征，可大致将研究海区划分为三个生物水文学区域。 对菲律宾海上层水体的光场特征作了分析，结果表明水下辐照度Ed按照指数规律衰减。通过对漫衰减系数Kd的研究，首次证实了西菲律宾海非藻组分的光衰减作用占主要地位。表层水体中非藻组分与浮游植物的光衰减作用大小相近，随着水深增大，非藻组分的光衰减作用逐渐占据主导地位。此外，西菲律宾海离水辐亮度Lwn(555)和海面遥感反射率Rsr(555)同生物地球化学核心变量POC之间存在显著的指数相关关系，这对于海洋初级生产力的遥感估算和应用具有重要意义。

A new species, Gerres septemfasciatus (Perciformes: Gerreidae) from the Chinese coastal waters of the South China Sea

We describe a new species of gerreid fish, Gerres septemfasciatus, based on four specimens collected from the northern South China Sea. G septemfasciatus most closely resembles G limbatus in general appearance. However, G. septemfasciatus is distinguished from the latter and other congeners by having 3 to 3.5 scales between the base of. the fifth dorsal spine and lateral line. This species has a distinctive color pattern, including 7-8 regular, vertical, blue-grayish bands on its side. The distribution of this species is currently known to include the Chinese coastal waters of the South China Sea, but may be also include the coastal waters of southeastern Asia.

植物生长季海北高寒湿地辐射收支特征

依据祁连山海北高寒湿地植物生长期观测资料, 分析了区域辐射收支情况及其变化特征. 结果表明: 祁连山海北高寒湿地有较强的太阳总辐射(DR), 但地表反射辐射(UR)、地面有效辐射(ELR)较低;地表长波辐射(ULR)、大气逆辐射(DLR)、净辐射(Rn)均较高, 且具有明显的日、季节变化. 植物生长期5～9月的日变化振幅DR＞Rn＞UR＞ULR＞DLR＞ELR, 其总量表现出ULR＞DLR＞DR＞Rn＞UR＞ELR, 分别达4650.6、 4225.5、 3058.8、 2156.2、 477.6和425.1 MJ•m-2. 从月际分布来看, ULR、 DLR、 DR最高出现在7月, Rn出现在6月, UR出现在5月, ELR出现在8月, 分别为980.3、 913.1、 721.5、 554.9、 112.1和127.4 MJ•m-2.

Comparative study on CO2 emissions from different types of alpine meadows during grass exuberance period

Potentilla fruticosa scrub, Kobresia humilis meadow and Kobresia tibetica meadow are widely distributed on the Qinghai-Tibet Plateau. During the grass exuberance period from 3 July to 4September, based on close chamber-GC method, a study on CO2 emissions from different treatments was conducted in these meadows at Haibei research station, CAS. Results indicated that mean CO2emission rates from various treatments were 672.09+152.37 mgm-2h-1 for FC (grass treatment); 425.41+191.99 mgrn-2h-1 for FJ (grass exclusion treatment); 280.36+174.83 mgrn-2h-1 for FL (grass and roots exclusion treatment); 838.95+237.02 mgm-2h-1 for GG (scrub+grass treatment); 528.48+205.67 mgm-2h-1for GC (grass treatment); 268.97 ±99.72 mgm-2h-1 for GL (grass and roots exclusion treatment); and 659.20±94.83 mgm-2h-1 for LC (grass treatment), respectively (FC, FJ, FL, GG, GC, GL, LC were the Chinese abbreviation for various treatments). Furthermore, Kobresia humilis meadow, Potentilla fruticosa scrub meadow and Kobresia tibetica meadow differed greatly in average CO2 emission rate of soil-plant system, in the order of GG＞FC＞LC＞GC. Moreover, in Kobresia humilis meadow,heterotrophic and autotrophic respiration accounted for 42% and 58% of the total respiration of soil-plant system respectively, whereas, in Potentilla fruticosa scrub meadow, heterotrophic and autotrophic respiration accounted for 32% and 68% of total system respiration from G-G; 49% and 51%from GC. In addition, root respiration from Kobresia humilis meadow approximated 145 mgCO2m-2h-1,contributed 34% to soil respiration. During the experiment period, Kobresia humilis meadow and Potentilla fruticosa scrub meadow had a net carbon fixation of 111.11 grn-2 and 243.89 grn-2,respectively. Results also showed that soil temperature was the main factor which influenced CO2 emission from alpine meadow ecosystem, significant correlations were found between soil temperature at 5 cm depth and CO2 emission from GG, GC, FC and FJ treatments. In addition, soil moisture may be the inhibitory factor of CO2 emission from Kobresia tibetica meadow, and more detailed analyses should be done in further research.

Growth and milk performance of yak in southern Qinghai area

The Study carried out in Xueshan Township, Guoluo prefecture, Qinghai Province, P. R. China from 1997 to 1999 on 1) Growth regulation of yak from born to adult and 1/4 wild yak from born to one and half year; 2) The effect of nursing or not on the growth and development of dam; 3) Meat performance of local yak; 4)Milk productivity of female yak. It can be served as the theory basis from which crossbreed improvement of yak and government decision could be drawn.

全方位装配型AGV的工程应用

自动导引车(Automated Guided Vehicle)通常称AGV,自动导引车系统 (Automated Guided Vehicle System)通常称AGVS。AGV是一种无人驾驶搬运车,它可以按照监控系统下达的指令,根据预先设计的程序,依照车载传感器确定的位置信息,沿着规定的行驶路线和停靠位置自动行驶。AGVS由中央监控系统,AGV,通信系统,地面导引系统,装载、卸载站及其移载装置,充电站等组成。自动导引车系统广泛应用在仓库,工厂, 码头,机场中的自动化仓储系统,柔性加工,柔性装配系统,以