Acid-stable amperometric soybean peroxidase biosensor based on a self-gelatinizable grafting copolymer of polyvinyl alcohol and 4-vinylpyridine
Data(s) |
2001
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Resumo |
A peroxidase was extracted from Chinese soybean seed coat, and its thermostability and acid-stability were characterized. This peroxidase was immobilized into a self-gelatinizable grafting copolymer of polyvinyl alcohol with 4-vinylpyridine(PVA-g-PVP) to construct an acid-stable hydrogen peroxide biosensor. The effect of pH was studied for optimum analytical performances by amperometric and spectro-photometric methods, also the K-m(app) and the stability of the soybean peroxidase-based biosensor are discussed. At pH 3.0, the soybean peroxidase maintained its bioactivity and the enzyme electrode had a linear range from 0.01 to 6.2 mM with a detection limit of 1.0 x 10(-7) M. In addition, the main characteristics of different hydrogen peroxide sensors were compared. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Wang BQ;Li B;Cheng GJ;Dong SJ.Acid-stable amperometric soybean peroxidase biosensor based on a self-gelatinizable grafting copolymer of polyvinyl alcohol and 4-vinylpyridine,ELECTROANALYSIS,2001,13(7):555-558 |
Palavras-Chave | #HYDROGEN-PEROXIDE #HORSERADISH-PEROXIDASE #MODIFIED ELECTRODES #ENZYME ELECTRODE #MONOLAYER #SURFACE #FILM |
Tipo |
期刊论文 |