Vertical variations in kinetics of alkaline phosphatase and P species in sediments of a shallow Chinese eutrophic lake (Lake Donghu)

The vertical distribution of the variables relevant to P forms in sediments were studied in a shallow Chinese freshwater lake (Lake Donghu) in 1997, 1998, 1999 and 2000, to assess the contribution of enzyme to P availability in sediment cores. Sediment P was fractionationd into iron-bound P, calcium-bound P, acid soluble organic P (ASOP) and hot NaOH extractable residual organic P. The former two species made the largest contribution to the sediment P pool. All P species exhibited significantly higher concentrations in different depths at Station I, compared with those found at Station II, except for ASOP. Coupled with these lower ASOP concentrations, the V-max data of alkaline phosphatase, measured on the same samples, were significantly higher at station I. Taken together, ASOP were probably important in supplying the enzymatic substrate (Phosphatase Hydrolyzable Phosphorus, PHP) into interstitial water. Dissolved orthophosphate and PHP concentrations were highly heterogeneous , but peaked in subsurface, paralleled by higher V-max and lower K-m values of alkaline phosphatase, throughout the sediment core. Sediment in the eutrophic lake is not only enriched in available P (iron-bound P), or stores residual P, but also tends to release PHP, thereby inducing the production of alkaline phosphatase and releasing o-P into water column by enzymatic hydrolysis. The latter process may also occur in relatively deep sediment layers.

CONTRIBUTION OF BACTERIOPLANKTON, PHYTOPLANKTON, ZOOPLANKTON AND DETRITUS TO ORGANIC SESTON CARBON LOAD IN A CHANGJIANG FLOODPLAIN LAKE (CHINA)

The relative compositions of bacterioplankton, phytoplankton, zooplankton and detritus of seston were studied during the course of inundation in a floodplain lake of central Changjiang (China). Peaks in bacterial biomass developed shortly after flooding, coinciding with the initial leaching of organic nutrients from vegetation submerged under floodwater, and again at high water, shortly before the climax of phytoplankton biomass. Rods predominated the bacterial carbon biomass. Phytoplankton developed a postflood bloom at initial falling, corresponding to the drainage of the lake water into the river. While minimal biomass occurred during the advent of flooding, most likely due to disturbance and dilution. Algal biomass was usually dominated by Chlorophyta. Highest biomass of zooplankton was recorded at the end of the flooding in connection with the decline in turbidity, and once again at early drainage, closely associated with high phytoplankton biomass. Copepods (mainly nauplii) always constituted the majority of zooplankton carbon biomass. Peaks in detrital carbon concentrations were recorded at rising and falling water phases, corresponding respectively to the riverine discharge and decomposition of macrophyte mats. At rising water phase, CPOC was abundant. While during other water phases, this predominance was shifted to FPOC alone. Taken together, average contribution of bacterioplankton, phytoplankton, zooplankton and detritus to total seston carbon was 3.29, 21.21, 6.83 and 68.67 %, respectively.

Carbon film deposited by mass-selected low energy ion beam technique and ion bombardment effect

By mass-selected low energy ion beam deposition, amorphous carbon film was obtained. X-ray diffraction, Raman and Auger electron spectroscopy depth line shape measurements showed that such carbon films contained diamond particles. The main growth mechanism is subsurface implantation. Furthermore, it was indicated in a different way that ion bombardment played a decisive role in bias enhanced nucleation of chemical vapor deposition diamond.

大型制冷机组的冲洗与调试

大型制冷机组部件众多，管路比较复杂，又是必须承受高压的容器，安装、调试都有严格的要求。以30HXC165A制冷机组的现场冲洗、调试为例，详细介绍了大型制冷机组在试运行之前标准的准备工作。这些工作包括吹扫、试压、排污、充注制冷剂和冲洗调试等全部过程及注意事项。本文为大型螺杆式制冷机组这种压力容器设备的运行和维护管理提供了工程实践经验。结果表明：在施工调试过程中，只有各方互相协调，并且严格按照相关文件和规范要求才能顺利完成制冷机组的安装调试，为以后的正常运行打下基础。

水文条件对紫色土坡面土壤侵蚀及养分流失的影响

采用人工模拟降雨试验,研究水文条件对紫色土坡面土壤侵蚀及氮和磷养分流失的影响。试验处理包括2个施肥水平(低肥和高肥水平),4个水文条件(自由下渗、土壤水分饱和、壤中流、壤中流+降雨)和一个降雨强度(60 mm/h,历时60 min)。结果表明:壤中流+降雨和土壤水分饱和条件下的土壤侵蚀量分别是自由下渗条件下的3.1和1.7倍,同自由下渗相比,壤中流、壤中流+降雨和土壤水分饱和条件下,地表径流中NO3-N、HPO4-P的浓度和流失量有显著增加;低肥水平条件下,自由下渗、土壤水分饱和、壤中流和壤中流+降雨地表径流中,NO3-N的浓度分别是0.88、58.90、698.41和87.80 mg/L,对应水文条件下地表径流中,HPO4-P的浓度分别是0.252、0.322、0.811和0.383 mg/L,高肥水平条件下,径流中的NO3-N和HPO4-P的浓度也有相同的趋势;土壤水分饱和条件下,地表径流中NO3-N和HPO4-P的流失量分别是自由下渗条件下的27~39和1.3倍,壤中流+降雨条件下,地表径流中NO3-N和HPO4-P的流失量分别是自由下渗条件下的100~114和1.5~1.7倍,同时,壤中流+降雨和土壤...

称重式蒸渗仪系统改进及在农田蒸散研究中的应用

水分是制约黄土丘陵区农作物正常生长和农业生产的关键因子。该区年平均降水量少且季节多变,研究农田土壤水分平衡对该区旱地农业生产具有重要意义。土壤蒸渗仪(Lysimeter)可测量总蒸散量、渗漏量等有关水量平衡的各个分量,具有其它装置和方法不可比拟的优越性。它为田间蒸散和降水入渗实验研究提供了一种较先进的量测方法和技术手段。它对推动我国实验水文学的发展具有重要作用,并为相关领域的科学研究提供了一种较先进的技术设备和实验环境。中国科学院安塞试验站对原建造的用于测定农田蒸腾蒸发转化的称重式蒸渗仪在2005年对称重感应系统、电源进行改造,主要对主机、采集软件进行了重新更新,使其可直接在Windows界面下工作,数据采集更加方便,将测量出来的模拟量信号转换为数字信号,经处理显示并记录在数据采集器内。改进后蒸渗仪主要有以下特点:(1)土柱重19~26t,蒸渗仪精度为152 g(0.05 mm),可测量蒸腾蒸发量和地下水对土壤水的补给量与入渗量;(2)蒸渗仪面积3 m2(1.5 m×2 m),深度3 m,可充分允许农作物根系发育与吸水,土壤水和地下水转化,地下水位变化等过程进行,蒸渗仪的供排水系统能够在蒸渗仪内模拟实际地下水...

瑞丽江流域鱼类组成、区系及生活史特点研究

于2003年9月、2006年4月和11月,分别对瑞丽江流域鱼类资源进行了3次实地调查,共采集到鱼类49种,其中包含1个鱼类属级新纪录及新种——小眼小波鱼(Microrasbora microphthalma)。结合历史资料及文献整理,瑞丽江共有鱼类60种,隶属8目、19科、44属;其中,伊洛瓦底江水系特有鱼类16种,外来引入种9种。鱼类组成上,以鲤科鱼类最多,有26种,占总种数的43.3%;其次为鮡科,有11种,占总种数的18.3%。瑞丽江鱼类区系以喜温的"第三纪类群"中的山区鱼类为主要成分,上游杂有耐高寒的"青藏高原类群"成分,下游杂有热带江河平原鱼类。从生境、生活水层及食性探讨了瑞丽江鱼类生活史特点,并简要分析了鱼类的受危因素并提出了相应的保护建议。

Diamond growth by carbon ion implantation of diamond

Medium energy (5-25 keV) C-13(+) ion implantation into diamond (100) to a fluence ranging from 10(16) cm(-2) to 10(18) cm(-2) was performed for the study of diamond growth via the approach of ion beam implantation. The samples were characterized with Rutherford backscattering/channelling spectroscopy, Raman spectroscopy, X-ray photoemission spectroscopy and Auger electron spectroscopy. Extended defects are formed in the cascade collision volume during bombardment at high temperatures. Carbon incorporation indeed induces a volume growth but the diamond (100) samples receiving a fluence of 4 x 10(17) to 2 x 10(18) at. cm(-2) (with a dose rate of 5 x 10(15) at. cm(-2) s(-1) at 5 to 25 keV and 800 degrees C) showed no He-ion channelling. Common to these samples is that the top surface layer of a few nanometers has a substantial amount of graphite which can be removed by chemical etching. The rest of the grown layer is polycrystalline diamond with a very high density of extended defects.

日光温室地下滴灌土壤水分运动数值模拟

依据土壤水分动力学理论和地下滴灌土壤水分运动特征，在测定地下滴灌滴头出流特性、土壤水分运动参数、地表蒸发强度的基础上，引入作物根系吸水模型，建立了地下滴灌土壤水分运动数值模型，用有限单元法中的Galerkin法对模型进行数值求解，田间验证结果表明模型具有较高的精度。 研究发现，地下滴灌滴头流速随供水压力的增大而增加，呈幂函数关系；不同埋深对滴头流速基本无影响；而土壤初始含水量只在灌水开始时对滴头流速有一定影响，最终滴头流速趋于相同且稳定；地下滴灌滴头流速主要受供水压力和滴头孔径的影响。 运用matlab数值分析软件对土壤持水曲线的van Genuchten模型进行求参，所求VG模型计算的不同水势下土壤含水量计算值与田间实测值之间偏差较小，曲线回归模型相关指数高达0.95，能很好地表征土壤水分特征曲线。 建立了地下滴灌土壤水分运动数值模型，确定地下滴灌条件下具体模型应用的边界和初始条件，利用HYDRUS-2D软件进行有限元求解。运行结果表明所建立的数学模型和所采用的数值方法能够较好地模拟地下滴灌条件下土壤水分运动过程。 采用量纲分析法推导了日光温室地下滴灌条件下的土壤湿润模式模型，建立了湿润宽度和湿润深度的经验公式，与实际监测结果对比，土壤湿润宽度和湿润深度模型估计值与实测之间偏差较小，能准确的模拟地下滴灌条件下土壤湿润体的宽度和深度。

森林流域单元水文模型的构建与应用

目前全球缺水、水污染、洪涝灾害以及水土流失仍然非常严重，尤其在我国北方地区。流域水文模型可用来进行不同需水管理的情景分析，为解决我国水问题提供科学依据。分布式水文模型是流域水文模型的发展方向，具有显著特点：1）应用前景广泛，不仅可以模拟流域水文过程，还可以协助模拟泥沙或污染物的运移过程，为水利工程设计、水土保持、环境保护等领域提供技术支持；2）能够预测流域土地利用或气候变化下的流域水文响应过程变化，为管理部门提供决策支持；3）模型所需要的参数全部具有物理意义，可通过实际测量确定，适合模拟实测系列较短或是无观测流域的水文过程；4）对于目前国际水文界的前沿问题—水文尺度转换提供了一种有效的解决途径。 然而分布式水文模型还不完善，如1)真实性问题。对一些水文过程和边界条件还不确定。2)尺度转换问题。目前很少考虑尺度对参数有效性的影响。3)检验问题。还无法判断对有些难以测量的水文状态变量的模拟正确与否。4)计算时间和数据存储的问题。有些分布式水文模型虽然具有很强的水文物理基础和完善的模型结构，但是计算时间过长和(或)数据存储过大，难以应用。上述问题的核心就是对分布式水文模型的核心—单元水文模型的研究不够，需要为进一步完善单元水文模型进行研究。 本文采用饱和入渗理论、Saint-Venant方程、Richards方程、Penman-Monteith方程等等构建了以有限差分法求解的适用于森林流域的单元水文模型，并通过实验室模拟试验和坡地径流场资料进行了验证，主要结论为： 通过不同坡度和不同雨强下的室内坡面产汇流实验模拟，表明：该模型模拟的坡面流和壤中流过程与实测过程基本一致，峰现时间、径流历时、峰值流量、出流总量模拟值与实测值的相对误差均较小，基本小于10%。模型的模拟精度较高，实用性较强，为深入研究壤中流机制和改进流域降雨-径流模型提供了理论依据。 通过坡地径流观测场实测资料的验证，表明：该模型模拟的坡面流过程精度较高，累计流量的精度更高于小时过程的精度，离差系数、效率系数、确定系数均较理想，具有应用价值，有助于改善分布式水文模型在森林流域的模拟效果。

日光温室渗灌土壤水分运动数值模拟

土壤水动力学参数的推导和土壤水分运动的数值模拟是研究土壤水分运动规律的两个重要部分，是农田节水灌溉自动化控制工程设计的理论依据。本文以曰光温室草甸棕壤的三种质地为研究对象，应用土壤转换函数理论和有限元法，通过RETC软件由土壤机械组成和容重两个基本物理性质指标推导其土壤水动力学参数；运用HYDRUS-2D软件对同一渗灌管埋深〔30cm）、3种管径、10种供水压力、6种初始土壤水势条件下的土壤水分运动进行了数值模拟；并对土壤水动力学参数推导和土壤水分运动数值模拟结果进行了实测验证。结果表明：（1）RETC和HYDRUS-2D是进行土壤水动力学参数推导和土壤水分运动数值模拟的有力工具。（2）土壤水动力学性质对渗灌条件下土壤水分运动速度具有显著的作用，最适宜渗灌的土壤是壤土，偏砂、偏虱质地次之。（3）渗灌的供水压力对三种质地土壤水分运动具有显著的影响。壤砂土、壤土和戮土三种质地土壤的适宜供水压力应分别不小于500cmH2O、700cmH2O、400cmH2O。（4）就内径为10mm、15mm和20mm渗灌管而言，渗灌管管径越大越有利于渗灌水向渗灌管四周土壤入渗，三种质地土壤渗灌均宜采用20mm管径渗管。（5）在壤砂质土和在较干燥的（土壤水势值＜-1000cmH2O）壤质土和赫质土中，土壤初始水势值对渗灌土壤水分运动数值模拟结果的影响较小，而在较湿润的（土壤水势值＞-1000cmH2O）壤质土和载土中，随着土壤初始水势值的降低，渗灌土壤水分运动速度（模拟）加快。

Investigation of high cycle and Very-High-Cycle Fatigue behaviors for a structural steel with smooth and notched specimens

The high cycle and Very-High-Cycle Fatigue (VHCF) properties of a structural steel with smooth and notched specimens were studied by employing a rotary bending machine with frequency of 52.5 Hz. For smooth specimens, VHCF failure did occur at fatigue cycles of 7.1 x 10(8) with the related S-N curve of stepwise tendency. Scanning Electron Microscopy (SEM) was used for the observations of the fracture surfaces It shows that for smooth specimens the crack origination is surface mode in the failure regime of less than 10(7) cycles While at VHCF regime, the material failed from the nonmetallic inclusion lies in the interior of material, leading to the formation of fisheye pattern. The dimensions of crack initiation region were measured and discussed with respect to the number of cycles to failure. The mechanism analysis by means of low temperature fracture technique shows that the nonmetallic inclusion in the interior of specimen tends to debond from surrounding matrix and form a crack. The crack propagates and results to the final failure. The stress intensity factor and fatigue strength were calculated to investigate the crack initiation properties. VHCF study on the notched specimens shows that the obtained S-N curve decreases continuously. SEM analysis reveals that multiple crack origins are dominant on specimen surface and that fatigue crack tends to initiate from the surface of the specimen. Based on the fatigue tests and observations, a model of crack initiation was used to describe the transition of fatigue initiation site from subsurface to surface for smooth and notched specimens. The model reveals the influences of load, grain size, inclusion size and surface notch on the crack initiation transition. (C) 2010 Elsevier Ltd. All rights reserved

Study on Water Resources Regulation and Reservoir Operating Prediction for Suppression of Salt Water Intrusion in the Pearl River Estuary

Salt water intrusion occurred frequently during dry season in Modaomen waterway of the Pearl River Estuary. With the development of region's economy and urbanization, the salt tides affect the region's water supply more and more seriously in recent years. Regulation and allocation of freshwater resources of the upper rivers of the estuary to suppress the salt tides is becoming important measures for ensuring the water supply security of the region in dry season. The observation data analysis showed that the flow value at the Wuzhou hydrometric station on the upper Xijiang river had a good correlation with the salinity in Modaomen estuary. Thus the flow rate of Wuzhou has been used as a control variable for suppression of salt tides in Modaomen estuary. However, the runoff at Wuzhou mainly comes from the discharge of Longtan reservoir on the upper reaches of Xijiang river and the runoff in the interval open valley between Longtan and Wuzhou sections. As the long distance and many tributaries as well as the large non-controlled watershed between this two sections, the reservoir water scheduling has a need for reasonable considering of interaction between the reservoir regulating discharge and the runoff process of the interval open watershed while the deployment of suppression flow at Wuzhou requires longer lasting time and high precision for the salt tide cycles. For this purpose, this study established a runoff model for Longtan - Wuzhou interval drainage area and by model calculations and observation data analysis, helped to understand the response patterns of the flow rate at Wuzhou to the water discharge of Longtan under the interval water basin runoff participating conditions. On this basis, further discussions were taken on prediction methods of Longtan reservoir discharge scheduling scheme for saline intrusion suppression and provided scientific and typical implementation programs for effective suppression flow process at the Wuzhou section.

核素131I胆道腔内辐照支架的制作和动物实验及临床应用研究

恶性梗阻性黄疸(malignant obstructive jaundice,MOJ)发现时多为晚期,如不治疗生存期仅3个月。由于肿瘤位置特殊、患者一般情况较差,仅5%～20%的患者可行外科切除和分流手术,并且手术死亡率相当高,分流术病死率高达10%～43%。1974年Molnar和Stocknm首先采用PTCD术（经皮肝胆管内外引流术，Percutaneous Transhepatic Cholangic Drainage,PTCD）,使MOJ患者的临床症状得到缓解, 获得进一步治疗的机会。1989年金属支架开始应用于胆道系统,恢复了胆汁的生理性引流,并使大多数患者拔除引流管,提高了生活质量,成为非手术治疗MOJ的首选方法。但肿瘤生长是造成支架堵塞、黄疸复发的主要原因。文献报道MOJ患者放置金属支架后,支架堵塞的概率达20%～86%,其中大多数由肿瘤生长通过支架网眼或超过支架边缘引起。因此,在支架置放的同时如何积极控制肿瘤生长成为提高疗效的关键问题。MOJ的局部治疗方法有多种,但都存在不足之处。国内学者采用动脉化疗栓塞结合PTCD或支架置放的方法治疗MOJ,使患者的生存期得到延长。但引起MOJ的胆管癌、淋巴结转移癌、胰腺癌、壶腹癌多为少血供肿瘤,而且由于血供特殊,大部分病例碘油沉积欠佳,因此动脉化疗栓塞对这类肿瘤的作用是有限的。外辐照治疗对胆管癌、淋巴结转移癌、胰腺癌、壶腹癌等引起MOJ的肿瘤有一定疗效,并与胆道引流术结合应用于上述肿瘤引起的MOJ。由于瘤体周围有肝脏、胰腺、胃肠道、肾脏等对射线敏感的器官, 限制了外辐照剂量,影响了疗效的提高。近几年,一种斩新的治疗手段正在应用于临床，就是通过PTCD手术时建立的通道再行腔内辐照治疗(核素内辐照治疗),可以较好的控制肿瘤继续生长，抑制支架再狭窄的发生,可使MOJ的治疗取得更好的效果。本研究的目的就是探索胆道内辐照支架制作的可行性，并设计特别的施源器，其具体方法可以简述为：以球囊扩张器扩张重度狭窄胆管+置入特制的二腔单囊管+腔内低剂量率放疗+腔内热频热疗+胆管引流等联合方法治疗。同时，就该二腔单囊管内辐照支架的安全性进行动物实验，从分子生物学、核医学的角度阐述了实验研究的机理和病理改变，同时进行了临床的应用研究，探讨胆道支架发生再阻塞的相关因素及对策，进一步提高（MOJ）患者的生存期。本工作结果总结如下： 1 对放射源和施源器均进行了改进，放射源为液态放射性核素131I，施源器为可调节的长柱形球囊，是专门设计的硅橡胶二腔单囊管，其功能为：中腔通接胆管腔（蓝色），具有引流胆汁作用；另一腔管连接球囊（绿色），是长柱状形可调节长短的球囊，囊内装填放射性液态核素131I；囊内还设置射频加热电极，能加热升温。另外，还可以根据肿瘤的大小和长度，来调节施源器的长度（长柱形球囊），一般照射长度超出支架两端1-2cm。二腔单囊管内辐照支架的特点：剂量分布合理，低剂量率效应，射线能量适中，放疗、热疗同步实施，引流、扩张和局部用药作用。与其他治疗方法比较，这一核医学治疗技术的最大优点是：大直径柱状液态源，近似面源，液囊适形病变胆管腔，均匀贴紧病灶壁，剂量分布合理，γ射线能量适中，低剂量率持续照射，同时联合射频温热治疗及胆道引流、局部用药对症处理等。 2 动物实验研究结果指出，囊装液态131I支架对胆管壁的放射性损伤随131I放射性活度的增加而逐渐加重,呈现明显的放射性活度效应关系。而普通支架组胆管壁无放射性损伤,但是胆管壁粘膜层和肌层增生较131I支架组重,胆管出现再狭窄倾向。本研究为临床合理应用131I支架治疗胆管癌及胆管良性狭窄选择合适的131I放射性活度,提供了必要的剂量学和基础研究依据。 3 动物实验研究结果表明,131I支架组犬胆管组织Fas表达较普通支架组明显,且其表达水平的变化与犬胆管壁平滑肌细胞凋亡的检测结果相同。131I支架组胆管腔面积比普通支架组明显增加,胆管狭窄程度比普通支架组轻。这主要是由于131I辐射促进Fas 基因表达,诱导增殖性平滑肌细胞凋亡,从而减轻胆管损伤后愈合过程中的管腔狭窄。 4． 胆道恶性梗阻胆道腔内囊装液态131I辐照治疗施源的直径为8mm时是胆管充分扩张的最低限度,根据近距离放疗的剂量学特点,它能保证胆管癌腔内辐照治疗时将施源管因素导致的放疗反应和局部肿瘤复发减至最低。并具有创伤小,操作简单,疗效明显,易被患者接受的特点。 5．囊装液态131I治疗恶性梗阻性黄疸（肝门部胆管癌18例），临床疗效满意，随访7～21个月,3个月生存率83.33 %(15/ 18)；6个月生存率72.22%(13/18)；9个月生存率56.25%(12/16)；12个月生存率46.15%(6/13),15个月生存率36.33%(4/11)；18个月生存率22.22%(2/9),21个月生存率28.57%(2/7)。4例发生并发症,其中2例为放射性胆管炎胆道出血,1例为胆汁性腹膜炎,另1例是胰瘘胰腺炎

2-D and 3-D processing and interpretation of multi-fold ground penetrating radar data:a case history from an archaeological site

A 2.5-D and 3-D multi-fold GPR survey was carried out in the Archaeological Park of Aquileia (northern Italy). The primary objective of the study was the identification of targets of potential archaeological interest in an area designated by local archaeological authorities. The second geophysical objective was to test 2-D and 3-D multi-fold methods and to study localised targets of unknown shape and dimensions in hostile soil conditions. Several portions of the acquisition grid were processed in common offset (CO), common shot (CSG) and common mid point (CMP) geometry. An 8×8 m area was studied with orthogonal CMPs thus achieving a 3-D subsurface coverage with azimuthal range limited to two normal components. Coherent noise components were identified in the pre-stack domain and removed by means of FK filtering of CMP records. Stack velocities were obtained from conventional velocity analysis and azimuthal velocity analysis of 3-D pre-stack gathers. Two major discontinuities were identified in the area of study. The deeper one most probably coincides with the paleosol at the base of the layer associated with activities of man in the area in the last 2500 years. This interpretation is in agreement with the results obtained from nearby cores and excavations. The shallow discontinuity is observed in a part of the investigated area and it shows local interruptions with a linear distribution on the grid. Such interruptions may correspond to buried targets of archaeological interest. The prominent enhancement of the subsurface images obtained by means of multi-fold techniques, compared with the relatively poor quality of the conventional single-fold georadar sections, indicates that multi-fold methods are well suited for the application to high resolution studies in archaeology.