survivin表达对重离子诱导人肝癌HepG2细胞生物学效应的影响

放射治疗是肿瘤三大治疗手段之一（手术治疗、放疗、化疗），如何提高肿瘤细胞的放射敏感性一直是科研人员关注的研究方向。电离辐射导致细胞死亡的主要方式是细胞凋亡，然而肿瘤细胞内往往细胞凋亡信号通路异常，降低了治疗效果。其中细胞内高水平表达的细胞凋亡抑制蛋白（Inhibitor of Apoptosis Protein，IAP）抑制了caspase分子的活性，而caspase分子正是细胞凋亡的执行分子。因此科学家们通过各种手段尤其是RNA干涉的方法以抑制肿瘤细胞内细胞凋亡抑制蛋白的表达及蛋白活性来达到提高肿瘤治疗效果的目的。 Survivin是凋亡抑制蛋白家族的一员，该蛋白在大多数恶性肿瘤中高表达，而在正常组织中检测不到，因此具有组织特异性。Survivin参与肿瘤细胞分化并抑制肿瘤细胞凋亡，它的高表达被证明与很多恶性肿瘤对放射治疗中产生的辐射抗性相关。本文主要研究了不同LET射线辐照下人肝癌HepG2细胞 survivin的表达及其表达对重离子诱导的生物学效应的影响。首先，我们使用不同LET的碳离子束和X射线辐照HepG2细胞，采用标准克隆形成法确定其辐射敏感性，利用流式细胞技术（FCM）检测辐射后细胞周期分布，RT-PCR和western blotting检测survivin的表达。结果显示，人肝HepG2癌细胞经不同LET射线照射，survivin表达是不同的。与低LET的X射线相比，高LET碳离子诱导的细胞损伤和周期阻滞更明显，从而诱导了更强烈的survivin表达。 接着，根据Genbank提供的survivin序列，合成特异性survivin-siRNA寡核苷酸，转染HepG2细胞，抑制survivin的表达。我们发现siRNA转染后诱导了细胞G2/M期阻滞，增加了自发性和辐射诱导的细胞凋亡。在碳离子辐照后，siRNA细胞克隆存活率明显下降。这些结果显示survivin表达是细胞产生高LET辐射抗性的关键因素。最后，我们初步探讨了在细胞凋亡过程中，survivin基因的作用机制。发现抑制survivin表达，对离子束辐射诱导的Bcl-2和Bax表达没有明显的影响。Survivin表达直接抑制了caspase-3和-9的活性，从而抑制了细胞凋亡。以上的实验结果表明：不同LET射线辐照细胞后survivin出现差异表达，与X射线相比，高LET重离子诱导的HepG2细胞中survivin表达更明显，以survivin为靶基因的siRNA技术应用于HepG2细胞，可以极大提高该细胞对重离子辐射的敏感性。本论文研究为临床应用重离子束治疗癌症提供了非常有用的基础数据，同时也为重离子束放射治疗联合基因治疗提供了新的思路

《内蒙古白音敖包自然保护区沙地云杉林生态系统研究》简介

沙地云杉林是陆地上非常特殊的森林生态系统类型，在世界上只分布于我国浑善达克沙地东部边缘，号称八百里瀚海的生态环境脆弱地区．由于它的存在为我国东北的西部和内蒙古东部沙地改良和三北防护林体系建设提供基因库和造林科学依据．《沙地云杉林生态系统研究》是由徐文...

A mathematical model of crevice and pitting corrosion—I. The physical model

A predictive and self-consistent mathematical model incorporating the electrochemical, chemical and ionic migration processes characterizing the propagation stage of crevice and pitting corrosion in metals is described. The model predicts the steady-state solution chemistry and electrode kinetics (and hence metal penetration rates) within an active corrosion cavity as a function of the many parameters on which these depend, such as external electrode potential and crevice dimensions. The crevice is modelled as a parallel-sided slot filled with a dilute sodium chloride solution. The cavity propagation rates are found to be faster in the case of a crevice with passive walls than one with active walls. The distribution of current over the internal surface of a crevice with corroding walls can be assessed using this model, giving an indication of the future shape of the cavity. The model is extended to include a solid hydroxide precipitation reaction and considers the effect of consequent changes in the chemical and physical environment within the crevice on the predicted corrosion rates. In this paper, the model is applied to crevice and pitting corrosion in carbon steel.

陕北水蚀风蚀交错区两种生物结皮对土壤饱和导水率的影响

该文以陕北水蚀风蚀交错区普遍发育的地表和地上两种生物结皮为研究对象,分别以3种非生物结皮(无结皮、物理结皮、去除生物结皮)为对照,使用盘式入渗仪测定其饱和导水率。结果表明:与无结皮土壤相比,两种类型生物结皮均可极显著降低土壤饱和导水率;与去除生物结皮土壤相比,两种类型生物结皮对土壤饱和导水率的降低均不显著;与有物理结皮发育的土壤相比,地表生物结皮对土壤饱和导水率的降低不显著,而地上生物结皮对土壤饱和导水率的降低显著。一方面,两种生物结皮对土壤饱和导水率均有明显降低作用,预示生物结皮在降雨活动中可能会增加径流、降低入渗,阻碍研究区水分亏缺条件下的植被恢复和生态与环境建设。另一方面,与不同的对照相比,生物结皮对土壤饱和导水率的影响截然不同,该结论可在一定程度上解释当前有关生物结皮影响土壤水分入渗方面所存在的分歧。

硫脲及抑制剂组合对土壤尿素氮转化和玉米产量的影响

采用培养试验和田间小区试验相结合,研究了两种浓度硫脲及低用量硫脲和硝化抑制剂双氰胺(DCD)、脲酶抑制剂苯基磷酰二胺(PPD)组合对土壤脲酶活性、土壤尿素氮转化和玉米产量的影响。培养试验表明,硫脲及抑制剂组合对土壤脲酶活性有显著的抑制作用,抑制时间为2周。TU、TU1+DCD和TU1+PPD,对土壤NH4+-N的释放、NH4+-N向NO3--N的进一步转化有显著的抑制作用,进而影响土壤中速效氮的总量变化。田间试验表明,施用抑制剂显著增加了玉米百粒重和产量,增产幅度为9.14%1~1.49%。

小城镇无害化资源化水处理技术研究与应用

针对我国水资源短缺和小城镇水环境污染的恶化趋势 ,概述了适用于在小城镇实施的污水无害化、资源化处理技术及发展现状 ,提倡对小城镇污水进行无害化处理后进行资源化利用 ,因地制宜地发展小城镇污水处理技术 ,还就无害化、资源化水处理技术在我国中小城镇应用前景进行了分析与展望

中国省际煤炭资源流动的时空演变及驱动力

根据1957-2005年中国各省区煤炭调出和调入数据,运用SPSS和GIS方法,首先对"一五"～"十五"各省区的煤炭流动地域类型进行了判别,然后在省域尺度研究了煤炭资源区域流动的时空过程,最后探讨了煤炭资源流动时空演变的驱动力。"一五"～"十五"中国省际间煤炭流动演变特征有:中国省际间煤炭资源流动规模逐年增大,省际间煤炭调出总量年均增长5.9%,煤炭调入总量年均增长5.6%。流动范围逐年扩展,无流地由1957年的10个省区缩小至近年的1个左右。流场呈集中输流、分散汇流的特征,调出省区个数<调入省区个数,调

21世纪初中国生态退耕的空间格局及黄土高原典型区退耕的生态位适宜性分析(英文)

自1999年我国开始试行以"退耕还林"工程为主的生态退耕以来,到目前已历时十年。特别是进入21世纪,工程实施强度较大,因此从国家尺度上进行宏观生态退耕格局的分析对于评估生态退耕政策和确定未来政策导向具有重要意义。本研究以TM遥感监测的土地利用变化数据库为基础分析2000-2005年生态退耕的时空格局。在此基础上,以黄土高原典型地区陕西中北部为研究区,采用生态位适宜度评价模型探讨了生态退耕工程实施的合理性。研究结果表明:(1)2000-2005年间,生态退耕主要集中在中国中部地区,尤其是在黄河和长江的中上游地区,生态退耕面积与建设占用面积基本持平。在各生态类型区中,黄土高原土壤侵蚀区生态退耕面积最大,达到了1162.50km~2,主要用于还林;(2)整体看来,研究区内生态退耕的空间格局是合理的,即适宜性越差的耕地退耕比例越大。从退耕区域的适宜性水平来看,有77.35%的地区属于勉强适宜区,其中退为林地的面积为603.32km~2,退为草地的面积为528.94km~2。整个研究区内前者大于后者。然而,退耕地中仍有19.38%属于中度适宜区,原因可能来自于退耕指标分配等管理因素,另外,当地农民外出务工从而将中等适宜度的耕地退耕。结果表明,当前仍有大面积不适宜和勉强适宜的耕地存在,在未来工程实施过程区应该首先考虑该类地区。

Microstructure and mechanical properties of Mg-4.5Zn-xNd (x=0, 1 and 2, wt%) alloys

Microstructure and mechanical properties of Mg-4.5Zn-xNd (x = 0, 1 and 2, wt%) alloys heat-treated at 603 K for 2 It have been investigated. T-phase (an Mg-Zn-Nd ternary phase) was observed in the Nd containing alloys. The optimal mechanical properties were obtained in the Mg-4.5Zn-1Nd alloy, and the ultimate tensile strength and yield strength were 228 and 79 MPa, respectively. Through comparing with the Mg-4.5Zn alloy, the increments of ultimate tensile strength and yield strength were 51 and 17 MPa.

Laser glazing of lanthanum magnesium hexaaluminate

Lanthanum magnesium hexaalumminate (LMA) is an important candidate for thermal barrier coatings due to its thermal stability and low thermal conductivity. On the other hand, laser glazing method can potentially make thermal barrier coatings impermeable, resistant to corrosion on the surface and porous at bulk. LMA powder was synthesized at 1600 degrees C by solid-state reaction, pressed into tablet and laser glazed with a 5-kW continuous wave CO2 laser.

Computer simulation of Gd(III) speciation in human interstitial fluid

The speciation and distribution of Gd(III) in human interstitial fluid was studied by computer simulation. The results show that at the background concentration, all the Gd(III) species are soluble and no precipitates appear. However as the total concentration of Gd(III) rises above 2.610 x 10(-9) mol/l the insoluble species become predominant. GdPO4 is formed first as a precipitate and then Gd-2(CO3)(3). Among soluble species, free Gd(III), [Gd(HSA)], [Gd(Ox)] and the ternary complexes of Gd(III) with citrate as the primary ligand are main species when the total concentration of Gd(III) is below 2.074 x 10(-2) mol/l. With the total concentration of Gd(III) further rising, [Gd-3(OH)(4)] begins to appear and gradually becomes a predominant species.

Study on the phase behavior of BaEu2Mn2O7 through heat treatment of a single crystal

A new orthorhombic phase of BaEu2Mn2O7 with the space group of Ccmm (no.63) was identified for single crystals after heat treatment and its Crystal Structure was determined by single crystal X-ray diffractometry. The volume Of the unit cell has twice the fundamental tetragonal cell and corner-shared MnO6 octahedra are slightly distorted and Mn-O-Mn angle between the neighboring octahedra tilts with an angle by around 3 degrees from b-axis. It is concluded from the results of the heat treatment of single crystals at various temperatures that this orthorhombic phase changes into a tetragonal One With superstructure (P4(2)/mnm) at 402 K and changes once more into the fundamental tetragonal phase (I4/mmm) above 552 K. The tetragonal phase with superstructure which has been expected to be an unstable one is stable between the two temperatures.

Confining conducting polyaniline in a stable inorganic network

Water soluble conducting polyaniline with electrical conductivity of 10(-1)-10(-2) S/cm was prepared employing dopant induced water solubility technology. The water resistance of the conducting film was significantly improved employing,sol-gel hybrids method, especially when the conductive polyaniline loading was below 30 wt%. The reason for the improvement is that the conducting polyaniline chains are confined in a stable inorganic network.