88 resultados para Eco, Humberto
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作为常用的除草剂和氮肥,乙草胺、尿素在黑土区广泛应用。本文就乙草胺和尿素单一及复合污染对黑土农田的生态毒理效应及机理进行研究,为黑土区农业持续发展提供理论参考。乙草胺和较高浓度的尿素均对蛆蝴有毒害作用,可导致蛆叫体重下降及死亡;污染胁迫引起生物体生内产生大量O2-等自由基,抗氧化酶系中两种关键酶SOD和POD活性受活性氧自由基诱导而应激性升高,可溶性蛋白含量下降,过高浓度则将导致酶系崩溃,生物死亡。乙草胺和较高浓度的尿素均对小麦和白菜的发芽、根伸长及幼苗生长有毒害作用,可降低发芽率,抑制其根、芽的伸长;乙草胺导致小麦叶片叶绿素和可溶性蛋白含量降低,对SOD和POD活性则表现为先诱导后抑制;低浓度尿素能增加小麦仆卜绿素及蛋白质含量,提高SOD和POD活性,但高浓度导致小麦叶片叶绿素、可溶性蛋白含量及SOD活性降低,POD活性进一步升高,影响生物膜的生物合成及完整性,使植株生长受阻,甚至枯死。乙草胺对黑土土壤微生物有不同程度的抑制作用,低浓度下可较快恢复,高浓度与此相反。乙草胺、尿素均为低浓度促进黑土呼吸作用,浓度升高则促进作用减弱。二者的联合作用多呈现为低浓度尿素可部分拮抗乙草胺的毒性作用,高浓度尿素则强化乙草胺的毒害作用,表现为协同作用。
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为了对沈阳市绿化树种的合理配置及城市森林环境效益的定量评价提供科学依据,本文以沈阳典型城市森林功能类型为试验区,利用L工一6400光合作用测定系统,采用自然条件下定位观测和室内分析相结合的方法,在典型城市森林功能类型主要绿化树种光合与蒸腾生理生态特性分析的基础上,首次对沈阳市城市森林固碳释氧和降温增湿生态环境效益进行了系统研究。典型城市森林功能类型主要树种的光合与蒸腾日变化曲线多呈单峰型,春季和夏季公园主要树种以双峰型曲线居多。光合速率与蒸腾速率季节变化趋势主要表现为夏季>秋季>春季。利用多元逐步回归分析方法,首次建立了沈阳市典型功能类型主要树种光合速率和蒸腾速率与生理生态因子的关系模型,确定了各季节影响光合与蒸腾速率的主要因子。典型城市森林功能类型乔木树种单位叶面积日固碳释氧能力为:白榆>京桃>旱柳>银中杨>刺槐>油松>银杏,降温增湿能力为:白榆>京桃)银中杨>旱柳>刺槐>油松>银杏;灌木树种日固碳释氧与降温增湿能力均为:金钟连翘>重瓣榆叶梅>紫丁香。碳氧平衡分析表明,沈阳市城市森林年总固碳量205.20万吨,总释氧量147.88万吨,沈阳市耗氧量为1 463.04万吨,森林释氧量为其耗氧量的10.11%。
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在糖化学中,糖原酸酯是一类重要的合成中间体,广泛运用于1,2-反式糖苷的合成,尤其对于寡糖的立体选择性合成具有重要的价值。目前文献报道的制备糖原酸酯的方法大多存在对环境不友好的问题。本文对传统的糖原酸酯制备方法进行了改进,通过研究发现无机碱也能够有效地催化合成糖原酸酯。以溴代糖和醇(或糖基受体)为原料,在无机碱、四丁基溴化铵、乙腈的体系中,合成了一系列简单醇糖原酸酯和糖-糖原酸酯。 聚乙二醇及其衍生物作为有机反应的溶剂和催化剂在有机化学中有广泛的应用。本文阐述了一种以溴代糖和醇(或糖基受体)为原料,在无机碱和聚乙二醇二甲醚反应体系中合成糖原酸酯的方法。该方法中,聚乙二醇二甲醚即作为绿色溶剂又作为催化剂,反应条件温和、环保、高效。 糖胺是一类重要的糖苷酶抑制剂,已在糖尿病和其他代谢紊乱等疾病的治疗中发挥了极其重要的作用。本文提供了一种合成一类具有潜在的糖苷酶抑制活性、结构新颖的二环糖胺的途径。该合成思路是以1-叠氮基-2-C-乙酰甲基-3,4,6-三-O-苄基-2-脱氧-β-D-葡萄糖为原料,经二环糖亚胺中间体,通过二环糖亚胺还原或加成得到一类二环糖胺。 Sugar orthoesters as one of the most important intermediates in carbohydrate chemistry, are used extensively in the synthesis of sugar 1,2-trans-glycosides, especially oligosaccharide. These methods in the literature are mostly eco-unfriendly. Herein we described a modified protocol for the preparation of sugar orthoesters using inorganic base, by improving the conventional method. Our method involves the treatment of peracetylated or perbenzotlated glycosyl bromides with alcohols in the presence of a quaternary ammonium salt and an inorganic alkali in acetonitrile solvent, affording both simple sugar orthoesters and sugar-sugar orthoesters. Polyethylene glycol and their derivatives as solvents or catalysts play a significant role in the organic reaction. We developed a novel and environmentally benign methodology towards the synthesis of sugar orthoesters, which are prepared by the reaction of peracetylated or perbenzotlated glycosyl bromides and alcohols in the presence of dimethyl ether of polyethylene glycol as either the reaction medium or catalyst. Glycosylamines and pseudo-glycosylamines have been tested against various glycosidases, and applied to the treatment of diabetes and other metabolic disorders. We presented a route of the synthesis of a bicyclic glycosylamine as a potential glycosidases inhibitor with unique structure. Reduction of 2-C-acetlymethyl-β-glucopyranosyl azide derivative firstly produced a bicyclic glycosylimine intermediate, and subsequently the bicyclic glycosylamine and its derivatives would be prepared through the selective reduction or addition the C=N double bond of the bicyclic glycosylimine intermediate.
Resumo:
青藏高原东缘的亚高山针叶林是长江上游重要的生态屏障,经过近六十年的采伐后,取而代之的是大量人工种植的云杉纯林。目前,这些人工林已经表现出树种单一,结构层次简单等生态问题,其物种多样性及生态效益与同地带天然林相比差距较明显。如何丰富该地区物种多样性,完善人工林生态系统的生态功能是一个十分重要的课题。林下植物是人工林群落的重要组成部分,对维持群落的生物多样性及完善生态系统功能具有明显的作用。因此,研究该地区人工针叶林的林下植被对不同生境的适应性对于理解人工林生态系统物种多样性的形成和维持机制都具有重要的意义。 本文以青藏高原东部亚高山针叶林的主要森林类型----云杉人工林为研究对象,选择林下11种具有不同喜光特性的常见植物,分别设置人工林林冠下及成熟林窗为研究样地,通过对各种植物叶片形态与物质分配特征、叶片解剖学特征、叶片光合生理特性、植物自然分布特征等方面的比较分析,研究林下植物对不同光生境的适应策略及其适应能力,揭示不同物种对人工林生境的适应共性,为西南亚高山地区植被恢复及人工林的经营管理提供科学依据。具体研究结果如下: 在叶片形态和物质分配特征方面:在林窗光生境中,11种林下植物叶片比叶重(LMA)显著高于林下光生境的同种植物。同时,林窗下生长的植物叶片叶片厚度及栅栏细胞长度显著增加,这是影响叶片比叶重变化的直接原因。而多数植物叶重比在两种生境中无明显变化。说明在长期适应自然生境之后,植物可能更多地采取调节叶片组织细胞水平(即叶片功能细胞形态)及叶片器官水平(即单个叶片形态)特征的策略来适应各类生境,而非整株水平上的叶片总比重的增减。 在叶片解剖结构特征方面:多数阔叶物种栅栏组织厚度(PT)、栅栏组织厚度/海绵组织厚度(PT/ST)、栅栏细胞层数及近半数种的气孔密度(SD)在林窗生境中更大或更多,而叶片表皮细胞厚度(UET、LET)气孔长径(SL)及海绵组织厚度(ST)受两种生境影响不大。喜光特性相似的物种在生境适应策略上具有一定的趋同性。 在光合生理特征方面:在林窗生境中多数种植物的最大光合速率(Amax)、暗呼吸速率(Rd)及喜光植物光补偿点(LCP)显著或极显著高于林内生境同种植物。且在同一生境条件下,多数深度耐荫植物比喜光及轻度喜光植物有稍低的Rd和LCP。各植物在林内低光生境中具有更大的内禀光能转化效率,并在中午12:00~14:00之间光强最大的时刻发生了的最深程度的光抑制。多数种能通过调节自身某种光合素含量或色素之间的比例来适应不同的光生境,即通过增加叶绿素含量或降低Chla/b值来适应林内弱光生境,通过提高类胡萝卜素含量或单位叶绿素的类胡萝卜素含量降低强光带来的伤害。绝大多数物种并不采取调节叶片C、N含量的策略来适应不同的光生境。总之,植物部分光合参数(Amax、Rd、LCP)受生境的影响与其自身喜光特性有关,但另一些参数(Fv/Fm日变化、色素含量及比例、叶氮相对含量)受生境影响与其自身喜光特性无明显关联。 在表型可塑性方面:在叶片各表型参数中,器官水平及细胞水平的形态特征参数平均可塑性大于整株水平形态和物质分配特征参数可塑性;叶片光合组织的可塑性大于非光合组织可塑性;反映植物光合能力的参数可塑性大于叶片色素含量参数可塑性。植物叶片形态和物质分配、解剖学特征参数平均可塑性大小与其自身喜光特性基本吻合,即喜光种及轻度耐荫种各参数可塑性最高,深度耐荫种可塑性最小,而这种规律并未在光合生理参数的可塑性大小上体现出来。但是叶片形态和物质分配参数、光合生理参数的平均可塑性水平却大于叶片解剖学参数。 在植物自然分布特征方面:喜光物种云杉幼苗及歪头菜在林内生境中分布密度明显降低,深度耐荫种疏花槭却恰恰相反,更多数物种(7种植物)在两种生境中密度变化趋势不明显。从分布格局来看,7种植物在两种生境中均为聚集分布,但聚集强度为林窗>林内;少数物种桦叶荚迷、直穗小檗、冰川茶藨、黄背勾儿茶在林窗中为聚集型,在林内生境中的分布型发生改变而成为随机型,说明光生境的差异能影响到植物种群的分布特征。但这种影响程度与植物自身的喜光特性无关,同时与各物种叶片表型平均可塑性的大小也无明显关联。 The subalpine coniferous forest area in eastern Qinghai-Tibet Plateau is important ecology-barrier of upriver Yangtze. In past sixty years, those forests had been cut down and replaced with a lot of spruce plantations. At now, there are many ecology problems presenting to us such as singleness species, simple configuration, lower species diversity and ecological benefit than natural forests at the same belt. How to restore the species diversity and enhance the eco-function of the plantations is a very important issue. The understory plants are important part of plantation community, which improved the bio-diversity and eco-function distinctly of forests. So, it is very significance to study the adaptation of understory plants to different environment in plantation, and this study would helping us to understand how plantations to develop and remain their biodiversity. This study was conducted in a 60a spruce plantation in Miyaluo located in western Sichuan, China, and spruce plantation is major types of subalpine coniferous forest in eastern Qinghai-Tibet Plateau. In this paper, the leaf morphological and biomass-distributed characteristics, the anatomical characteristics, the photosynthetic characteristics and the distribution patterns characteristics of eleven different light-requirement understory species grown in two different environments (forest gaps and underneath close canopy) were studied and compared. The purpose of this study was to analyze the adaptation of this forest understory plants, to show up the commonness of these different light-requirement understory species in light acclimation, and to provide some scientific reference to manage and restore the vegetation of subalpine plantation of southwest China. The results were as follows: The leaf morphological and biomass-distributed characteristics: These eleven species in forest gaps had significantly higher dry weight per leaf area (LMA) than those under close canopy. The palisade parenchyma cells of the broad-leaved species in gaps were significantly longer than those grown under the canopy, which been a directed factor for the change of leaf mass per unit area (LMA) in different environment. But the leaf weight ratio (LWR) of most plants species were not evidently changed by the contrasted environments in our study. It was shown the morphological characteristics changing been adopted as a strategy of light acclimation for plants wasn’t on whole plant level (leaf weight ratio) but cellular level (the function cells morphological characteristics) and organic level (the leaf morphological and biomass-distributed characteristics) mostly. The leaf anatomical characteristics: Most broad-leaved plants in gaps increased palisade parenchyma thickness (PT), the palisade parenchyma cell layers and the ratio of palisade to spongy parenchyma (PT/ST). So did as almost about half species in this study in stomatal density (SD). No significant differences in thickness of leaf epidermal cells (UET, LET), stomatal length (SL) and spongy parenchyma (ST) between two environments of most species were observed. The results suggested that species with light-requirement approximately had convergent evolution on adaptation to light condition. The leaf photosynthetic characteristics: The dark respiration rate (Rd) of most plants species, the light compensation point (LCP) of light-demanding plants species in gaps were significantly increased than under close canopy in this study. In a same habitat, most deep-shade-tolerant plants had lower Rd and LCP than those light-demanding plants and slight-shade-tolerant plants. Each species has bigger inherent electron transport rate under close canopy than in gaps, and the greatest photoinhibition happened during 12 to 14 in the daytime. Most species could adapt different light environment by the way of changing their photosynthetic pigments content or the ratio of pigments content. For example, some plants under close canopy increased chlorophyll (Chl) or reduced the values of the ratio Chla/b to adapted the low light condition, some plants in gaps increased carotenoid (Car) or reduced the weight ratio CarChl to avoid been hurt in high light. For most plants, changing the content of C and N in leaf wasn’t a strategy of light acclimation. In conclusion, the variation of some leaf photosynthetic parameters in different light environment such as Fv/Fm, pigments, C and N in leaf related with the light-requirmnet of species, but the others such as Amax, Rd, LCP did not. The leaf plasticity indexes: Among those leaf plasticity indexes, the leaf morphological and biomass-distributed parameters on cellular and organic level were greater than on whole plant level for same species, and the photosynthetic parenchyma parameters were greater than non-photosynthetic parenchyma parameters in same leaf, and photosynthetic capability parameters were greater than photosynthetic pigments content parameters for same species. The average plasticity indexes of leaf morphological and biomass-distributed and anatomical parameters were accordant with plants’ light-requirement approximately: those light-demanding plants and slight-shade-tolerant plants had bigger plasticity indexes than deep-shade-tolerant plants. But this regular wasn’t observed in physiological plasticity indexes for most plants, though the average leaf plasticity indexes of leaf morphological and biomass-distributed, photosynthetic characteristics parameters was greater than the anatomical characteristics parameters. The distribution patterns characteristics: Oppositely to the deep-shade-tolerant specie Acer laxiflorum Pax., the density of light-demanding species Picea asperata Mast. and Vicia unijuga A. Br. in gaps was bigger than under close canopy. Each of the other species has the approximately density in two different environment. The spatial patterns of seven species were aggregated distribution in two environments, but the trend of aggregation of population under close canopy was decrease from in gaps. A few species such as Viburnum betulifoium Batal., Berberis dasystachya Maxim., Ribes glaciale Wall. and Berchemia flavescens Brongn. were aggregated distribution in gaps while random distribution under close canopy. It was shown that the difference between two light environments could affect the distribution pattern of plant population, and the effect didn’t relate with the light-requirement or plasticity indexes of species.
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西南地区在我国的经济发展和生态环境建设中占重要地位,但也是我国生态环境最脆弱的地区之一,生态系统退化,生态功能减弱,严重制约着西南林业的可持续经营与发展。本项目采用DNA 分子标记SSR 研究不同生境条件下粗枝云杉群体的遗传变异及其时空分布格局,考察遗传变异与复杂的山地生态环境间的潜在联系,系统地揭示粗枝云杉天然群体与环境系统相互作用的生态适应与分子进化机制。粗枝云杉适应性强,生长迅速,在植树造林和工业用材方面占有重要地位,研究成果可为中国西南部亚高山天然林的可持续经营及退化生态系统的恢复与重建提供理论依据和科学指导。主要研究结果如下: 1. SSR 位点变异丰富,等位基因频率的分布格局多样。7 个SSR 标记全是多态位点,每位点的等位基因数变化范围为13~24,平均为19.9 个。SSR 位点的等位基因片段长度范围变化较大。73.1%的等位基因变异遵循逐步突变模型(SSM)而发生1 个重复基元的变化,22.3%和4.6%的变异分别按两阶段突变模型(TMP)发生1 个重复基元以上的变化和在SSR 位点侧翼区发生1 个碱基变化的插入-删除事件。 2. 粗枝云杉拥有中等偏高水平的遗传多样性和相对大的群体间遗传分化。通过分析代表10 个群体的250 个个体在7 个SSR位点的变化,调查了源自中国西南山区的粗枝云杉的微卫星变异。相当高的遗传多样性和强烈的群体分化发生在粗枝云杉中, 其群体平均Nei's 期望杂合度为0.707 , 群体间遗传距离为0.121~0.224(FST)和0.100~0.537(RST)。然而,群体间遗传距离与地理距离之间无相关性,从而排除了简单的距离分离模式并暗示迁移不是影响粗枝云杉遗传变异格局的主要因素。事实上,使用私有等位基因估算的基因流数量非常低,仅等于0.753。等位基因置换检验(Allele permutation tests)揭示逐步突变及遗传漂变都对群体间分化有贡献。另外,在多数位点检测到显著的群体间遗传差异,这个结果说明自然选择,假设通过环境压力,是引起粗枝云杉微地理分化的主要因素之一。根据SSR基因型,250 个粗枝云杉个体的70%被正确地归类入其各自的来源群体,结果表明微卫星(SSR)对区分来自中国不同生态地理位点的粗枝云杉基因型是有效的。 3. 在SSR、RAPD 和AFLP 位点,显著的群体间遗传结构被发现的,但三种标记间遗传分化程度和群体遗传关系有差异。利用来自10 个群体的247 个个体,我们报告了关于样本粗枝云杉群体间遗传关系的总体看法。根据各自对评价遗传关系的信息能力和适用性,SSR、RAPD 和AFLP 标记被选用,三种技术非常有效地区别这些基因型。使用的SSR、RAPD 和AFLP 标记分别估计平均Dice 相似性系数。Mantel 检验产生显著但相对低的共表型适合度(RAPD = 0.63£AFLP = 0.60和SSR = 0.75)。比较三种标记系统,RAPD 和AFLP 共表型指数相对高地相关(r =0.59),而RAPD 和SSR 及SSR 和AFLP 之间的相关系数分别是0.53 和0.35。所有系统树,包括不同标记资料结合获得的系统树,反映了多数群体依据它们的地理条件而成某种特定关系。结果暗示单个或结合标记系统能用来深入洞察粗枝云杉遗传研究,并且不同标记系统合并资料能提供更可靠的信息。 Southwestern region plays an important role in economic developmentand ecological construction in China. Yet, it is also one of the weak regionsof ecological environment in China with degraded ecosystem and imperfectfunction, which restricts the sustaining management and development ofsouthwestern forestry. The genetic variation and spatial distribution patternof P. asperata populations originating from different habitats wereinvestigated using SSR molecular markers in this study. The correlationsbetween genetic variation and ecological and environmental conditionswere detected, and the interaction between P. asperata populations andenvironmental system and the mechanism of ecological adaption -molecular evolution were revealed. Given the significant ecological andeconomic roles of the fast-growing and wide-adaptive species in reforestation and production of pulp wood and timber, the study couldprovide a strong theoretical evidence and scientific direction for thesustaining management of subalpine natural forest, and the afforestationand rehabilitation of degraded ecosystem. The results are as follows: 1. The genetic variation at SSR loci was abundant and the distributionof allelic frequencies was uneven. All seven loci were polymorphic, and thenumber of alleles per locus varied from 13 to 24 with a mean valueequaling 19.9. The allele sizes at SSR loci were found to vary widely.73.1% of allelic variation followed stepwise mutation model (SSM) whichresults increase or decrease by one repeat type, and 22.3% and 4.6% wereresulted from two-phase mutation model (TMP) with allele size varying bymore than one repeat type and from insertion-deletion events in theflanking regions at SSR loci with a single basepair changing, respectively. 2. P. asperata possessed a moderate to high level of genetic diversityand considerable genetic differentiation. Microsatellite variation of P.asperata. originating from the mountains of southwestern China wasinvestigated by analyzing variation at seven SSR loci in 250 individualsrepresenting ten populations. A fair degree of genetic diversity and strongpopulation subdivision occurred with the mean gene diversity (H) of 0.707,and genetic distances among populations varying between 0.121 and 0.224(FST) and between 0.100 and 0.537 (RST). However, inter-populationgenetic distances showed no correlation with geographic distances between the population sites. This ruled out a simple isolation by distance modeland suggested that migration does not have a great impact. In fact, theamount of gene flow, detected using private alleles, was very low, equalingonly 0.753. Allele permutation tests revealed that stepwise-like mutations,coupled with genetic drift, could contribute to population differentiation.Moreover, significant genetic differences between populations weredetected at most loci. The results indicate that natural selection, presumablythrough environmental stress, may be one of the main factors causingmicro-geographical differentiation in the genetic structure of P. asperata.Based on SSR genotypes, 70% of the 250 individuals were correctlyclassified into their sites of origin. This suggests that microsatellites (SSRs) are effective in distinguishing genotypes of P. asperata originating fromdiverse eco-geographical sites in China. 3. Using a set of 247 individuals from ten P. asperata populations wereport an overview on the genetic relationship among the sampled P.asperata populations. RAPD, AFLP and SSR were used in terms of theirinformativeness and applicability for evaluate relationship and all threetechniques discriminated the genotypes very effectively. Mean Dicesimilarities coefficient were estimated using RAPD, AFLP and SSR,respectively. The Mantel test resulted in a significant but relatively low fit(RAPD = 0.63, AFLP = 0.60 and SSR = 0.75) of cophenetic values.Comparing the three marker systems to each other, RAPD and AFLP cophenetic indices were highly correlated (r = 0.59), while correlationcoefficient between RAPD and SSR was r = 0.53 and between SSR andAFLP was r = 0.35. For all markers a relatively high similarity indendrogram topologies was obtained although some differences wereobserved. All the dendrograms, including that obtained by the combineduse of all the marker data, reflect some relationships for most of thepopulations according to their geographic conditions. The results indicatethat single or combined marker system could be used to insight into geneticstudy in P. asperata and the combined data of different marker systems canprovide more reliable information.
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常绿阔叶林以其富饶的生物资源、丰富的生物多样性和巨大的生态与环境效益引起了人们越来越大的重视,它的研究已成为国际植被科学界关注的主题之一。我国分布着世界上面积最大的亚热带常绿阔叶林,在世界植被中具有重要地位,它的分布表现出明显的地带性差异,存在着多样的植物群系及其对应的气候特征。但是在植物功能性状领域,与全球范围其它生物群系相比,常绿阔叶林物种的研究较少,其功能性状间、功能性状与环境间的关系尚不清晰。 本研究以常绿阔叶林木本植物的当年生小枝为对象,试图从小枝水平上的生物量分配格局、叶片大小与数量的权衡关系、小枝茎的构型效应、叶片元素化学计量学,以及小枝大小的成本与效益分析等方面,较为系统地揭示小枝水平上的植物功能性状间及其与气候间的关系。因此,在华西雨屏带内部的不同纬度设置峨眉-青城-雷波-平武的温度梯度进行比较,并对有降水差异的川西南偏湿性(雷波)与偏干性常绿阔叶林(西昌)进行对比研究,同时在不同山体进行不同海拔梯度的比较研究。 本文主要研究结果如下: (1)小枝生物量分配格局叶水平上,叶片重-叶柄重(Y轴vs.X轴,下同)呈斜率小于1的异速生长关系,表明叶柄对叶内部的生物量分配影响显著。小枝水平上,叶和茎的生物量以及它们与小枝总生物量间基本呈等速生长关系,表明大的小枝或大叶物种不一定在叶生物量的分配上占优势。不同生活型间,在小枝或者茎的生物量一定时,常绿物种叶片的生物量比例较落叶物种稍高。与温度和水分较优越(峨眉及其低海拔)的生境相比,在相对低湿(螺髻)与低温(平武)的生境中的植物会减少对叶的投入而增加对支撑部分的投资比例。 (2)小枝叶片大小与数量的权衡无论是不同气候带还是不同生活型以及不同海拔梯度,叶片大小与出叶强度基本都是呈负的等速生长关系,表明了叶片大小-数量在小枝水平上的权衡。在不同气候梯度的对比中,叶片数量(出叶强度)一定时,高温和高水分生境(峨眉)比低温(平武)和低湿(螺髻山)生境中的物种的叶片大小(质量和面积)更大,表明不同生境的比较中,小的叶片可能具有较高的出叶强度和更高的适合度收益。“出叶强度优势”(Leafingintensitypremium)假说可能不适宜解释不同生境物种叶片大小差异。 (3)小枝茎的构型效应虽然茎长和茎径与叶片大小都呈正相关关系,与出叶强度都呈负相关关系,但茎长/茎径比与叶/茎生物量之比呈负相关关系;与叶片的大小呈负相关关系,与出叶强度呈正相关关系。这说明小枝构型能影响小枝叶/茎生物量分配和叶大小-数量的权衡关系。其影响机制可能是小枝内部的顶端优势。另外,茎长/茎径比在低湿和低温等不利生境中的植物中较高,而在降水和温度较适宜环境中较低。 (4)叶片C、N、P化学计量学N含量和P含量,C/N比和比叶重(LMA,leafmassperarea)呈正的等速生长关系,而N和LMA,P和LMA呈负的等速生长关系。在LMA一定时,C/N比随着生境胁迫压力的增加而降低,N、P含量随着生境压力的增加而增加。在P含量一定时,N含量随着生境压力的增加而降低,即N/P比在生境条件较优(峨眉及其低海拔)时较高。常绿和落叶植物叶片的N/P比没有差异,在LMA一定时,常绿植物的N、P含量较高、C/N比较低。总之,植物的C、N、P化学计量学特征受叶片属性如LMA与气候,及其相互作用的影响。 (5)小枝大小的代价与效益分析、TLA与小枝总重总叶面积(TLA,totalleafarea,Y轴,下同)与总叶重(X轴)均呈斜率小于1的异速生长关系,TLA与小枝横切面积呈斜率为1的等速生长关系。表明叶片面积的增加总是小于叶重和小枝总重的增加,随着小枝的增大,它的叶面积支撑效率下降。在热量和降水优越的生境(峨眉及其低海拔)中,相同小枝重或者相同茎横切面积的小枝,其叶面积支撑效率较低湿与低温环境下(螺髻山、平武及高海拔)的高。 总体上,本文初步研究了小枝水平上可能存在的以下三种权衡关系:叶-茎生物量分配权衡;叶片大小-数量的权衡;小枝茎长-茎径的权衡关系,以及气候要素等对这三种权衡关系的影响。在此基础上,我们还讨论了这些权衡关系的可能形成机制,及其与物种生态适应的联系。本研究丰富了生活史对策中关于权衡关系的研究内容,为我国常绿阔叶林功能生态学研究积累了材料。 Evergreen broad-leaved forests are attracting much more attention from vegetation ecologists than ever before because of their abundant nature resource and biological diversity, and also great ecological benefits. China has the largest distribution of subtropical evergreen broad-leaved forests (temperate rainforests) that are typical and representative in the world. The forests span over more than ten degrees in latitude and more than 30 degrees in longitude, providing an ideal place to study plant functional ecology, i.e., the climatic effect on plant functional traits and the relationship between the traits. However, relative to the other biomes, there are few studies addressing functional ecology of the plant species from subtropical evergreen broad-leaved forests. In this study, I focused on the leaf size-twig size spectrum of the woody species of subtropical evergreen broad-leaved forests in southwestern china. I collected data on leaf size and number, twig size in terms of both mass and volume, and stem architecture from five temperate mountains, and then I analyzed the relationships between leaf and stem biomass and between leaf size and number, the effect of stem length/diameter ratio on biomass allocation and on the relationship between leaf size and number, leaf C:N:P stoichiometry, and the twig efficiency of supporting leaf area in relation to twig size. I also addressed the climate effect on the spectrum. The temperature gradient from warm to cool sites was represented by Emei Mountain, Qingchengshan, Leibo, and Pingwu, and the rainfall gradient was assumed to emerge from the comparison between Leibo (High) and Luojishan (Low). In addition, altitudinal effects were analyzed with comparisons between low and high altitudes for each mountains. My main results are as follows. Isometric relationships were found between leaf mass and twig mass and between lamina mass and twig mass, suggesting that the biomass allocation to leaves or laminas was independent of twig mass. Petiole mass disproportionably increase with respect to lamina mass and twig mass, indicating the importance of leaf petioles to the within-twig biomass allocation. In addition, the investigated species tended to have a larger leaf and lamina mass, but a smaller stem mass at a given twig mass at favorable environments including warm and humid sites or at low altitude than unfavorable habitats, which might be due to the large requirements in physical support and transporting safety for the species living at unfavorable conditions. Moreover, the evergreen species invested more in leaves and laminas than the deciduous at given stem or twig biomass within any specified habitats. Negative, isometric scaling relationships between leaf number and size broadly existed in the species regardless of climate, altitude, and life forms, suggesting a leaf size/number trade-off within twigs. Along the climatic gradients, at given leaf number or leafing intensity, the leaves were larger in the favorable environments than the poor habitats. This suggested that the fitness benefit gained by small leaves could be larger than that with high leafing intensity in the stressful sites. I concluded that the “leafing intensity premium” hypothesis was not appropriate to interpreting between-habitat variation in leaf size. Both stem length and diameter were positively correlated to leaf size but negatively correlated to leafing intensity. The ratio of stem length to diameter was negatively correlated to leaf mass fraction, and it was negatively correlated to leaf size but positively correlated to leafing intensity. This suggested that the stem architecture influenced twig biomass allocation and the relationship between leaf size and number. The mechanism underlying the architectural effect might lie in the apical dominance within twig. Moreover, the ratio was greater in unfavorable habitats but smaller in favorable environments. Positive, isometric relationships were found between N and P contents per leaf mass, and between C/N ratio and leaf mass per area (LMA), but N and P contents scaled negatively to LMA. C/N ratio decreased but N and P increased with increasing habitat stress at a given LMA. N content declined with increasing habitat stress at given P content. These indicated that N/P and C/N were higher but LMA was lower in favorable habitats than in the other circumstances. The evergreen and deciduous species were non-heterogeneous in N/P, but the evergreen species have higher N and P contents and lower C/N than the deciduous ones. In general, C:N:P stoichiometry were related to both climatic conditions and other important functional traits like LMA. Total leaf area (TLA) allometricly scaled to leaf mass with a slope shallower than 1, similar to the relationship between TLA and total twig mass (leaf mass plus stem mass), suggesting that TLA failed to keep pace with the increase of leaf mass and twig size. However, TLA scaled isometricly to twig cross-sectional area. Thus, it could be inferred that the twig efficiency of displaying leaf area decreased with increasing twig size. In addition, the efficiency at a given twig size was large in favorable than unfavorable habitats. In general, in this preliminary study, I studied three tradeoff relationships within twigs, i.e., between leaf and stem biomass, between leaf number and size, and between stem length and diameter, as well as the climatic effect on the relationships. I discussed the mechanisms underlying the tradeoff relationships in view of biophysics and eco-physiology of plants. I believe that this study can serve as important materials advancing plant functional ecology of subtropical forest and that it will improve the understanding of life history strategies of plants from this particular biome.
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植物功能生态学研究不仅提供了植物生理生态学与生态系统生态学的连接,还为植物种群生活史对策研究提供了材料。Westoby 等 (2002) 提出了利用植物功能性状变量的主导维度来确定和量化植物生活史的生态适应策略。在他们所提出四个主导维度中,叶大小-小枝大小是研究相对较少的一维;其内部各组分的关系、对环境的响应,以及与其它重要维度的关系,目前的理解非常有限。 本研究以贡嘎山不同海拔不同功能群物种为研究对象,采用种间比较和系统发生独立性比较等研究方法,系统研究了植物的功能特征及其相关性在不同生境及不同功能群间的差异,旨在分析不同功能群物种的叶大小-小枝大小的成本和收益。其研究结果将有助于我们理解植物生活史对策的进化,进而理解物种共存和维持物种多样性的机制。主要研究结果如下: 1. 叶大小-小枝大小关系 小枝茎横截面积与单叶面积和总叶面积均呈异速生长关系,即总叶面积和单叶面积的增加比茎横截面积的增加速度快。但是,总叶面积和叶片干重的增加却基本上与小枝茎干重的增加等速。系统发生独立性比较研究的结果与此相一致。表明,在某一给定的茎投入时,至少大叶大枝物种不比小叶小枝物种在支撑叶面积和叶片干重方面具有优势。同时,在某一给定的小枝茎投入时,常绿阔叶物种比落叶阔叶物种支撑更少的叶面积。在茎干重与总叶面积的关系中,落叶复叶物种比落叶单叶物种具有更高的y轴截距,表明复叶物种比单叶物种在展叶面积方面更有效。复叶物种与单叶物种相比,通常具有较大的叶大小和小枝大小。 2. 叶大小-叶数量关系 叶大小与数量间在不同的叶片习性、不同的叶片形态以及不同的生境类型的物种间均存在稳定的负的等速生长关系,且这种关系在系统发生独立性比较时依然成立。然而,在某一给定的出叶强度 (单位小枝的叶数量) 时,常绿阔叶物种比落叶物种具有更小的叶面积。而在给定体积基础上的出叶强度时,落叶复叶物种的叶面积显著大于落叶单叶物种,且复叶物种比单叶物种具有更大的叶大小和更小的出叶强度。但是,叶大小与数量间的关系在不同的海拔间并没有显著的差异。 3. 小枝大小-总叶面积关系 在不同的生活型或不同的生境下,小枝上总叶面积与茎干重和小枝干重均呈正的异速生长关系,且斜率显著小于1.0,表明小枝上总叶面积的增加都不能赶上小枝及茎大小的增加。这种“收益递减”表明随着小枝干重的增加,光截取的收益递减。此外,叶面积比 (总叶面积与小枝干重的比值) 与单叶干重呈显著负相关关系,系统发生独立性比较的结果与此相一致。根据以上结果,可以推测,大叶的物种在质量较好的生境中出现,而群落内部小枝茎的寿命较长的物种可以拥有较大的叶片。 4. 叶片色素浓度-LMA关系 随着海拔的升高,阔叶木本植物和草本植物的叶片色素浓度减少,叶绿素a/b和类胡萝卜素/叶绿素比值以及比叶重 (LMA) 增加。然而,在草本植物中的色素浓度、色素比值和LMA的变化比阔叶木本植物的更明显。同时,LMA与叶片色素浓度呈负相关关系,但是在落叶物种中的LMA对色素浓度的影响比常绿阔叶物种更强烈。总之,草本植物的叶片特征对海拔梯度的变化似乎比木本植物更敏感,LMA对叶片色素的保护作用在落叶物种中比在常绿阔叶物种显得更重要。这些结果表明不同生活型物种可能采取不同的保护机制来降低叶绿体器官的损伤和增加他们的碳获取能力。 Studies on plant functional ecology not only bridge plant eco-physiology and ecosystem functioning, but also enrich plant population biology. As pointed out by Westoby et al (2002), plant life history strategies can be identified and quantified by four leading dimensions of variations in plant functional traits, i.e., seed size/output, leaf mass per area and leaf life span, plant height, and leaf size-twig size. Compared to the other dimensions, the cost/benefit of the leaf size-twig size spectrum has scarcely been analyzed in relation to environmental gradients and life form types, and the adaptive significance of this spectrum is not fully understood. In the present study, the relationships between functional traits of plant twigs are determined for the species with different life forms along an altitudinal gradient of Gongga Mountain with both cross-species analysis and evolutionary divergence analysis. The primary objective of this study is to examine the cost/benefit of leaf size-twig size in plants. The study results are supposed to provide insights into the understanding of the mechanism of species coexistences. The results are shown in the following. 1. The relationship between leaf size and twig size Twig cross-sectional area allometrically scaled with both individual leaf area and total leaf area supported by the twigs. However, the increase in total lamina mass/area was generally proportional to the increase in stem mass. These correlations between trait variations were significant in both interspecies analysis and phylogenetically independent comparison (PIC) analysis, which indicated that thick-twigged/large-leaved species, at least, do not have an advantage in supporting leaf/lamina area and lamina mass for the same twig stem investment than thin-twigged/ small-leaved species. Meanwhile, the evergreen broad-leaved species supported a smaller leaf area for the same twig stem investment in terms of both cross-sectional area and stem mass than the deciduous species. The deciduous compound-leaved species have a higher y-intercept in the scaling relationship of twig stem mass versus total leaf area than the deciduous simple-leaved species, indicating that compound-leaved species were more efficient in displaying leaf area. The compound-leaved species were larger in both leaf size and twig size than their counterpart in the present study. 2. The relationship between leaf size and leaf number Significantly negative and isometric scaling relationships between leaf size and leafing intensity (leaf number per twig mass or volume) were found to be consistently conserved across species independent of leaf habit, leaf form and habitat type. The negative correlations between leaf size and leafing intensity were also observed across correlated evolutionary divergences. However, leaf area was smaller in the evergreen broad-leaved species at a given leafing intensity than in the deciduous species. The deciduous compound-leaved deciduous species were higher in leaf area than deciduous simple-laved species at a given volume-based leafing intensity. Moreover, the compound-leaved deciduous species were larger in leaf size but smaller in leafing intensity than their simple counterparts. No significant difference was found in the scaling relationships between altitudes. 3. The relationship between twig size and total leaf area Leaf area was found to scale positively and allometrically with both stem and twig mass (stem mass plus leaf mass) with slopes significantly smaller than 1.0, independent of life form and habitat type, indicating that the increase in total leaf area fails to keep pace with increasing twig size and stem size. This ‘diminishing returns’ suggests that the benefit of light intercept decreased with increasing twig mass. Moreover, the leaf area ratio (the ratio of total leaf area to stem or twig mass) correlated negatively with individual leaf mass. The results of PIC were consistent with the correlations. According to the results, it is speculated that large-leaved species may be favored when habitat is good and when stem longevity are long within community. 4. The relationship between leaf pigment concentrations and leaf mass per area With increasing altitude, the concentrations of pigments decreased, but the ratios of chlorophyll a/b and carotenoid/chlorophyll, and LMA increased, in both the broad-leaved woody species and herbaceous species groups. However, the changes in the pigment concentrations, ratios and LMA were more profound in the herbaceous species than in the woody species. In addition, pigment concentrations were negatively correlated with LMA in each life form type and in the pooled dataset. However, the LMA effect on leaf pigment concentrations was more profound in the deciduous species than in the evergreen braode-leaved species. In general, herbaceous species seemed more sensitive to the increasing altitude compared to woody species, and LMA seemed to be a more important mechanism for protecting leaf pigments in deciduous species than in evergreen broad-leaved species. These results suggested that the species with different life forms may employ different protective mechanisms to decrease the chloroplast apparatus damage and increase their carbon gain.
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造纸行业是造成我国水环境有机污染物的重要污染源之一,其水污染的特点是小厂多、草浆多、工艺落后、污染扩散面广、造成废 水排放量大,每年排放的废水量约39亿立方米,占全国工业废水排放量的1/6,其中有机污染物(以BOD5计)160万吨左右,约占全 国工业废水中有机污染物总量的1/4。尤以占全国制浆造纸行业90%以上的碱法草浆造纸厂的蒸煮黑液量大面广,除含有机物外,还 含有木质素、残碱、硫化物、氯化物等污染物,属于PH值高、色度深、难于治理的高浓度有机废水,对水体污染特别严重,各地要 求治理呼声很高,急待研究并尽快找出各种有效的治理途径。对于碱法草浆蒸煮,黑液高浓度废水的治理,有各种方法,根据国内 的研究进展和我们已有试验工作表明,最经济有效,具有实用价值,在生产上可获得成功是厌氧处理法。近10多年来,国外关于高 效厌氧处理技术研究进展迅速,并出现了多种多样的工艺设备,如高效厌氧生物反应器,并在实用化方面取得了很大成绩,建立了 生产性装置,达到了高负荷运行,效果良好。本试验是根据我们已有研究基础,针对我国国情,对小型制浆造纸厂水污染防治除了 开发碱回收及各种综合利用技术外,要特别加强废水(废液)实用技术研究的指导思想,本试验采用改进型的上流式厌氧污泥床反应 器,设计了两种试验方案,通过试验结果如下。1. 试验方案I—碱法草浆黑液酸化和厌氧发酵I号UASB反应器动态模型试验结果表 明:(1). 采用中温35℃±1℃高效厌氧反应器USAB内装有填料(陶粒)和三相分离器,具有保持高浓度生物量和防止污泥流失的特点 ,污泥浓度Vs 可达30%以上,因而具有高效、节能、产能、滞留期短的优点,当进水CODcr在7500-10000mg/l,HRT由7天缩短到3天 ,有机容积负荷在1.22gCODcr/l·d-3.43gCODcr/l·d时,CODcr平均去除率可达55%-45.5%,最高CODcr去除率可达60.2-63.5%, BOD5去除率可达75.9-83.2%,沼气容积产气率可达0.29-0.67l/l·d,每克CODcr转化为沼气产率达0.39-0.48l/gCODcr·d,CH4含量 65.8-75.5%。厌氧发酵出水再用化学法进行后处理脱除难降解的木质素,CODcr总去除率达80%以上。(2). 动态试验结果表明:采 用酸化—厌氧发酵处理黑液工艺合理,技术路线可行。2. 试验方案II—黑液用化学法(Hcl)去除木质素进行厌氧发酵,II号UASB反 应器动态模型试验结果表明:(1). 采用中温35℃±1℃高效厌氧反应器UASB(内有软填料),当进水CODcr7000-13000mg/l左右,HRT 由6天缩短到1天,有机负荷由0.98gCODcr/l·d增加到11gCODcr/l·d时,COD平均去除率均可稳定在70-77%,BOD5去除率为87.3- 93.1%,沼气容积产气率0.21-2.6l/l·d,每克CODcr转化为沼气产率为0.39-0.48l/gCODcr·d,高的可达0.53l/gCODcr·d,转化 率较高,CH4含量63-70%。(2). 试验证明碱法草浆黑液物化预处理—厌氧发酵处理的技术路线也是可行的,工艺合理、效果较好。 在有条件的工厂可采用。3.厌氧发酵阶段几大类群微生物计数表明:(1). 当发酵工艺和运行处于相对稳定状态时,微生物群体的 组成也达到相对的稳定,各类微生物之间保持动态平衡关系。当产乙酸菌的数量为107-108个/ml时,产甲烷菌的数量为105-106 个/ml,当产乙酸菌数量为106-107个/ml时,产甲烷菌的数量为103-105个/ml。(2).稳态运行条件下,黑液预处理为甲烷发酵创造 了有利的生态环境,获得了较好的处理效果和较高的COD转化为沼气的产率0.39-0.48l/g·CODcr·d,反应器中形成较为稳定而数 量较下水污泥中高1-2个数量级的厌氧发酵微生物区系组成。这一结果为黑液厌氧发酵提供了微生物理论依据。Paper industry is one of the important pollution source of water environment in our country. Its character of water pollution is many small factories, much grass pulp, disadvantageous technique, large preading area of pullution. Its effluent makes up 1/6 of whole country's industry wastwater. Its organic pollutant accounts 1/4 of whole country's. Alkaline grass paper pulp effluent with pollutants such as ligoin, remaining alkali sulfide, chloride besides organic material, is a kind of high concentrate organic wastewater which has high PH walug, dark colour and is difficult in treatment. There is urgent require to find ways to treat the wastewater. There are different ways to treat alkaline paper grass pulp effluent. According to the research advances and our experiment work, the most economical and useful way is anaerobic degradation which was advanced quick in last ten years. In the control of waste water of small pulp paper mill, the study of wastewater utilization technology should be emphasized, besides alkaline retrieving and different kinds of comprehensive utilization technology. Our experiment used modified UASB(Upflow Anaerobic Sludge Blanket Reactor). Two kinds of plan were disgned. The results are lined below. 1. The first experiment plant-aciding black pulp effluent and methanogenic digestion. The dynamic model experiment results of I-UASB reactor showed: (1)The mesophilic(35℃±1℃)high effect UASB reactor having haydite and threee state seperation in it had the character of keeping high bioimass concentration and preventing losss of sludge. It had advantages of high effect, energe saving, energe prodcing and short HRT(Hydroulic retention time). When the influent COD was 7500-10000mg, HRT was shortened from 7 days to 3days, organic loading rate was 1.22g-3.43COD/l· d, the average COD removal efficiency was 55%-45%. The highest COD efficiency was 60.2-63.5%, BOD removal of 75.9 -83.4% was achieved. Biogass production rate were up to 0.29-0.67l/l·d. Biogass converted efficiency from every gram of COD could reach 0.39-0.48l/gCOD·d. Methane content was 65.0-75.5%. Chemical method was used to deplate lignin in anaerobic digestion effluent. Total COD removal efficiency could be more than 80%. (2)Using aciding annaerobic digestion to treat the black effluent was reseanable in technique and technology. 2. The second experiment plan-anaerobic digestion was used after the chemical method was used to deplate lignin in the black effluent. The result of dynamic experiment of II-UASB reactor showed: (1)High effect mesophilic (35℃±1℃)UASB reactor having soft slaffing in was used. When influent COD was about 7000-13000mg/l, HRT was shortened from 6 days to 1 day and organic loading rate was increased from 0.90 to 11g COD /l·d, average COD removal efficiency remained stable on 70-77%. BOD, removal efficiency was between 87.3-93.1%. Biogass production rate was 0.2-2.6l/l ·d .Biogass converted efficiency from a gram of COD was 0.39-0.481/gCOD·d with the high value of 0.53l/gCOD·d. Methane content was 63-70%. (2)The way that using physical, chemical Pre-treatment-anaerobic digestion to treat alkaline black effluent is feasible and can be used in some factories when the condition exists. 3. Counting of several class of microoganisms in anaerobic digestion stage showed: (1)As the disgestion was in stable motion, the compositon of microorganic colony could get relative stable. Dynamic balance was remaining among different kinds of microorganism such as methanogenic bacteria, Acidogenic bacteria, sulfate reducing bacteria, and heterotrophic bacteria. (2)Under stable motion, the pre-treatment of black effluent produced favourable eco-enviroment for methanegenic digestion. Good treatment effect and high biogass convertent efficiency from COD(0.39-0.48l/g·COD· d)were gotten. Some stable and high quantity(10-100times more than sewage sludge)microorganism colony were formed in the reactor. This result provided theoretical basis for anaerobic digestion of black effluent.
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综述了 60年代以来植物根际微域研究中土壤样品的采集方法 ,认为根际土样采集的发展趋势是从田间的粗略采集到室内模拟实验的精细划分 ,再把模拟装置运用到田间 ,最终达到指导生产的目的 .同时 ,随着根际概念的拓宽 (生态界面Eco boundarylayer) ,根际土样的采集朝着保持原位和更精细的方向发展 .
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以纸坊沟流域为例,构建了黄土丘陵区流域农业生态安全评价指标体系。依据该流域近70 a来的农业生态安全状况,将该流域生态经济系统演变过程划分为4个阶段。从这4个阶段入手分析影响农业生态安全的主要因子,得出了一系列评价指标。结合选取指标的科学性、动态性、实用性等基本原则,参考压力—状态—响应(PSR)模型和多位专家的建议,进一步筛选、分类和确定评价指标,从而建立了一套由生态环境、社会经济、综合功能3大类,20个具体指标构成的适宜于黄土丘陵区的农业生态安全评价指标体系。
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1991、 1996、 1997和 1998年的几次洪灾 ,造成了 6171亿元的直接经济损失。除气象因素外 ,水土流失是引发洪灾的一个极为主要的因素。加强领导 ,强化管理 ,加大执法力度 ,加强防洪工程建设 ,坚持综合治理 ,良化生态环境 ,搞好小流域治理是保持水土、治理江河的根本措施
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在国内外已有研究的基础上,结合黄土丘陵区纸坊沟流域近70a来农业生态经济系统演变过程,参考PSR模型,从生态环境、社会经济和综合功能3方面构建黄土丘陵区流域农业生态安全评价指标体系,基于层次分析法赋权的综合指数评价方法,对纸坊沟流域1938~2005年农业生态安全状况进行评价。结果表明:该流域农业生态安全状况发生了先降后升的变化,1938年处于较安全状况,1958年为极不安全状况,1975年和1985年都处于较不安全状况,1995年处于临界安全状况,2005年属于较安全状况。评价结果表明纸坊沟流域还需要进一步优化农业系统结构,实现农业生态安全。
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论述了水土保持与生态环境的关系 ,分析了水土流失对生态环境造成的严重影响和由此造成的经济损失 ,搞好水土保持不但可以改善生态环境 ,而且提高了人民的生活水平
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黄土高原丘陵沟壑区干旱缺水 ,要让有限的水资源支撑当地的生态农业建设 ,就要高效利用有限水资源 ,提高单位水的利用效率。通过在延安市燕儿沟的生产实践证明 ,在沟道中兴建截潜流工程 ,引水上山 ,发展坡地果园和保护地蔬菜微灌 ,是高效利用当地有限水资源的一种重要模式 ,很有推广价值
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对农业、环境建设、自然资源和农业生态经济“专家”系统建设等限制黄土高原生态农业建设和发展的有关的重大问题进行了理论分析 ,探讨了其存在的问题和运作设想 ,以求得在上述几个领域内为黄土高原生态农业、环境保护和经济的发展提供参考