1973年至2004年贵州喀斯特山区石漠化风险格局的变化——以普定后寨地下河流域中下游为例

以贵州省普定后寨地下河流域中下游为例,采用景观生态学的研究方法,利用多时相多数据源的遥感影像(1973年的航片、1995年的TM影像及2004年的SPOT-5影像),从不同等级石漠化风险的角度分析了研究区近30多年来的石漠化风险格局变化的特征,以期了解喀斯特高原山区的石漠化变化特征规律。结果表明:从景观水平上来看,整个区域的景观正在向程度更高的石漠化风险的方向发展,表现为极低度石漠化风险斑块的面积呈现出逐渐减少的趋势,而高度和极高度石漠化风险斑块的面积却呈现出不断增加的趋势;从斑块类型水平上来看,不同等级的石漠化风险斑块的变化差异明显,在变化幅度和变化趋势上都存在差异,其中极低度石漠化风险斑块面积变化最为明显,呈现出大幅度的下降,而变化最不明显的是低度石漠化风险斑块,无明显的增减趋势。

Quantified MS analysis applied to combinatorial heterogeneous catalyst libraries

A high-throughput screening system for secondary catalyst libraries has been developed by incorporation of an 80-pass reactor and a quantified multistream mass spectrometer screening (MSMSS) technique. With a low-melting alloy as the heating medium, a uniform reaction temperature could be obtained in the multistream reactor (maximum temperature differences are less than 1 K at 673 K). Quantification of the results was realized by combination of a gas chromatogram with the MSMSS, which could provide the product selectivities of each catalyst in a heterogeneous catalyst library. Because the catalyst loading of each reaction tube is comparable to that of the conventional microreaction system and because the parallel reactions could be operated under identical conditions (homogeneous temperature, same pressure and WHSV), the reaction results of a promising catalyst selected from the library could be reasonably applied to the further scale-up of the system. The aldol condensation of acetone, with obvious differences in the product distribution over different kind of catalysts, was selected as a model reaction to validate the screening system.

几种不同下垫面地表粗糙度动态变化及其对通量机理模型模拟的影响

在众多地表通量模拟模型和遥感通量反演模型中,空气动力学粗糙度(z0)是一个重要的地表参数．选取代表典型农田的禹城站,代表复杂下垫面的千烟洲站和代表森林下垫面的长白山站3个通量观测站的风速和温度廓线资料,运用最小二乘法拟合迭代,分别计算得到各站点通量塔所在地的零平面位移和空气动力学粗糙度．在此基础上,分别分析不同下垫面的空气动力学粗糙度随作物高度和叶面积指数(LAI)、风向(地形)、风速、摩擦速度等因子的变化．并采用SEBS模型分析地表空气动力学粗糙度动态变化对地表通量计算的影响．结果表明:空气动力学粗糙度随植被特征(如作物高度,叶面积指数等)以及风向、风速和摩擦速度等因子而变化．禹城和长白山站通量塔所在风浪区的空气动力学粗糙度明显随作物生长期植被高度及叶面积指数变化,即先随LAI增加而增加,达到峰值后,随LAI增加而减小;千烟洲叶面积指数变化较小,空气动力学粗糙度随叶面积指数的变化不明显;地形较平坦的禹城和长白山站空气动力学粗糙度随风向变化较小,而地形起伏较大的千烟洲站空气动力学粗糙度随风向变化较大．随着风速的变化,禹城站空气动力学粗糙度没有明显变化,而千烟洲和长白山空气动力学粗糙度表现出随风速增加而减小的趋势．各站空气动力学粗糙度的这种动态变化对模型通量反演有较大影响,通过模型分析, 5月1日～6月3日禹城空气动力学粗糙度日平均值、千烟洲及长白山通量塔空气动力学粗糙度5d平均值与模型所取z0值相比,由于z0的动态变化造成相同时间尺度显热通量H的计算相对误差的绝对值最大可分别达到2．726％,33．802％和18．105％．

不同水分状况下秸秆覆盖量对玉米根、冠生长的影响

在遮雨棚控水条件下,采用盆栽的方式研究了不同秸秆覆盖量对玉米根、冠生长的影响。试验按对照(CK)和秸秆覆盖量0.3 kg/m2(SM0.3)、0.6 kg/m2(SM0.6)、0.9 kg/m2(SM0.9)、1.2 kg/m2(SM1.2)共设5个处理,水分按高水(占田间持水量的90%)和低水(占田间持水量的60%)设两个水平。分别测定了玉米根、茎、叶、穗干重,根系垂直空间分布及叶片叶绿素相对含量等指标。结果表明:秸秆覆盖明显改变了玉米根系和地上部分的生长;高水条件下SM0.6处理玉米根系、茎秆、叶和穗干重均高于其它处理;低水条件下,地上部分干重SM1.2最大,SM0.6次之;根系的垂直空间分布与水分和覆盖条件都有密切的关系,覆盖措施主要影响下层根系的生长和发育。

土壤中Frankia菌RNA提取

土壤中Ｆｒａｎｋｉａ菌ＲＮＡ提取王育英，林建群，郭秀荣，李彤，张忠泽（中国科学院沈阳应用生态研究所，１１００１５）Ｆｒａｎｋｉａ非亚科共生固氮是一类重要的固氮资源。对Ｆｒａｎｋｉａ共生生物学、生态学等特性曾进行过广泛的研究。８ｔ０年代末，Ｆｒａｎｋｉ...

内蒙古陈巴尔虎旗饮茶型氟铝联合中毒调查

目的查明内蒙古陈巴尔虎旗饮茶型氟、铝联合中毒原因。方法在陈巴尔虎旗3个牧区嘎查随机抽取93名20~50岁牧民进行发、血清、尿含氟、铝水平测定,并拍摄前臂、小腿、骨盆正位及腰椎正侧位X线片,同时检测饮水、牛奶、砖茶等氟和铝水平,以扎兰屯市地方病防治研究所的34名23~50岁职工为对照组。结果陈巴尔虎旗牧民发、血清、尿及饮用的奶茶含铝量分别是(22.34±8.06)mg/kg、(1.13±0.48)mg/L、(3.78±1.62)mg/L、(6.02±1.77)mg/L,含氟量分别是(1.21±0.63)mg/kg、(0.092±0.042)mg/L、(2.08±1.16)mg/L、(3.00±1.11)mg/L,饮水、牛奶含铝量分别是(39±16)μg/L、(0.553±0.179)mg/L,含氟量分别是(0.50±0.39)、(0.063±0.032)mg/L;对照组发、血清、尿铝分别是(5.80±2.14)mg/kg,(0.203±0.101)mg/L、(2.19±1.34)mg/L,含氟量分别是(1.17±0.68)mg/kg、(0.033±0.008)mg/L、(0.57±0.32)mg/L,牧民发、血清、尿铝及血清、尿氟均高于对照组(P<0.001)。牧民X线改变硬化型22.2%(20/88),疏松型37.5%(33/88),退行性变86.4%(79/88),对照组退行性变13.3%(4/30),其余改变未检出。结论陈巴尔虎旗牧区饮茶型氟中毒实际为饮茶型氟、铝联合中毒。

A Simple and Inexpensive Method for Fabrication of Ultramicroelectrode Array and Its Application for the Detection of Dissolved Oxygen

Herein, we report a simple and inexpensive way for fabrication of ultra microelectrode arrays (UMEAs) and the relative characterization methods. The fabrication of UMEAs involves only a few steps of handwork. Since only metal wires and epoxy are used through the fabrication process, it is supposed to be a quite straightforward method for preparing UMEAs. A dissolved oxygen (DO) sensor based on UMEAs was constructed.

Investigation of the hydrolysis of ginsenosides by high performance liquid chromatography-electrospray ionization mass spectrometry

The hydrolysis of ginsenoside standards and the crude extracts of ginseng has been investigated at different pH values (2.4 - 11.2) using high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS). The experimental results indicated that the pH value of aqueous solutions is an important factor in changing the composition of ginsenosides. For (20S)-protopanaxadiol ginsenosides, ginsenosides with a large mass hydrolyzed to form hydrolysates (20S)-Rg(3) and (20R)-Rg(3) at pH 4.3. There were more hydrolyzed products observed at pH 3.3: (20S)-F-2, C-25,26 hydrated ginsenoside "C-Y-1" and "C-Y-2" (MW = 802 Da) accompanied with (20S)-Rg(3), (20R)-Rg(3). At pH 2.4, only (20R)-Rg(3), (20S)-F-2, a small quantity of (20S)-Rg(3) and three C-25,26 hydrated ginsenosides were obtained. For (20S)protopanaxatriol Re, no hydrolysates were observed at pH 4.3; it was hydrolyzed at pH 3.3 to form hydrolysates (20S)-Rg, (20R)Rg(2) and hydrated C-25,26 (MW = 802 Da) and at pH 2.4 only C-25,26 hydrated ginsenosides "C-Y-1" and "C-Y-2" (MW = 802 Da) were left in the solution. Similar hydrolysis reactions could be also observed for the crude extracts of ginseng. It showed that HPLC/ESI-MS is a fast and convenient method to study the hydrolysis of ginseng.

The luminescence of the phosphor Sr2ZrO4 with one-dimensional chains structure

A new type of phosphor Sr2ZrO4 with one-dimensional structure was prepared by solid reaction and its luminescence is seen at room temperature. The excitation and emission spectra were measured and display broad maximum at 354 nm and 386 nm respectively. The mechanism of this luminescence is ascribed to charge transfer.

In situ microscopic FTIR spectroelectrochemistry study of oxidation of L-ascorbic acid in polymer electrolyte (PEG/LiClO4)

In situ microscopic FTIR spectroelectrochemistry behavior of L-ascorbic acid (H(2)A) in polymer electrolyte is reported for the first time. H(2)A undergoes a two-step oxidation, The oxidation waves shift towards more anodic potential values when the scan rate increases. The peak currents of the oxidation waves are proportional to the square roots of scan rate up to 100 mV/s, The in situ infrared spectra suggest that the product of the oxidation be dehydroascorbic acid, which may exist as a dimer.

聚合物电解质(PEG/LiClO_4)中L-抗坏血酸氧化的现场显微红外光谱电化学研究

Ｌ－抗坏血酸（Ｈ２Ａ）由于在生物介质氧化还原过程中的重要作用，其分子及其衍生物已有广泛研究［１～５］．聚合物电解质由于能溶解电活性物质和具有离子导电性而成为一种新的溶剂，可用于研究一些物质的电化学行为［６］．Ｈ２Ａ在聚合物电解质中的电化学及光谱电化学…

Living planktonic foraminifera from the northwestern Pacific Ocean: SSU rDNA sequences and their paleoceanographic significance

Living planktonic foraminifera (PF) samples from the Okinawa Trough of the northwestern Pacific Ocean were taken for DNA analysis. The SSU rDNA sequences of two PF species, Globigerina sp. and Pulleniatina obliquiloculata collected at Station WP01, were obtained and compared with those from the southwestern Pacific Ocean. Only small differences (< 0.7%-1.2% for P. obliquiloculata, and 0.3% for Globigerina sp.) were found between samples from the north- and south-western Pacific Ocean areas and this molecular evidence supported that these micropaleontological species are the same species, which implies that the West Pacific Ocean circulation system influences the planktonic foraminiferal gene communication.

Ecdysteroid-responsive genes: E75 and Retinoid X Receptor (RXR), essential molt-regulating genes in the shrimp, Fenneropenaeus chinensis

本论文主要研究两种重要的调节蜕皮过程的基因—蜕皮激素效应基因E75和RXR在中国明对虾蜕皮中的作用。利用RT-PCR和RACE技术获得了编码FcE75和FcRXR的全长cDNA序列。FcRXR包含7个内含子，在对虾中存在不同的异形体，命名为RXR-1和RXR-2。应用荧光实时定量PCR分析表明FcE75和FcRXR基因在中国明对虾蜕皮前期（D3）其转录表达量明显上调。另外，FcE75和FcRXR基因在不同组织中的转录表达存在明显的差异。利用FcE75和FcRXR基因的双链RNA注射对虾能有效降低FcE75和FcRXR的表达水平。FcE75和FcRXR的体内沉默完全抑制了对虾的蜕皮过程，并且引起对虾的死亡。对不能正常蜕皮个体进行观察的结果表明，FcE75沉默的对虾，其上皮的收缩、新的刚毛及新表皮的形成均收到限制。在FcE75双链RNA沉默后的对虾中，我们检测了与蜕皮相关的一些效应因子，如chitinase等的转录，发现这些效应因子的转录明显受到抑制，说明FcE75和FcRXR在蜕皮过程中起到非常重要的作用。本论文首次阐明了这些基因在十足目甲壳动物蜕皮过程中的功能。

柴达木盆地弃耕地盐碱化治理途径初探

通过在柴达木盆地盐碱化弃耕地上种植多年生饲草和增施厩肥的试验结果表明：耕作层土壤发含量明显降低，土壤由重度盐碱土变为极轻盐碱化土壤；土壤有机质含量增加，土壤结构得到改善，弃耕地植被得到恢复，第一生产力提高。