177 resultados para Mixed fermentation


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The optimal entanglement manipulation for a single copy of mixed states of two qubits is to transform it to a Bell diagonal state. In this paper we derive an explicit form of the local operation that can realize such a transformation. The result obtained is universal for arbitrary entangled two-qubit states and it discloses that the corresponding local filter is not unique for density matrices with rank n = 2 and can be exclusively determined for that with n = 3 and 4. As illustrations, a four-parameter family of mixed states are explored, the local filter as well as the transformation probability are given explicitly, which verify the validity of the general result.

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Two biosensors for fermentation process control have been introduced, which were developed in our lab recently. One is an enzyme electrode-based on-line monitoring system for glutamate fermentation process control and the other is an H+-ISFET-based ENFET for penicillin G fermentation process control.

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The Raman scattering and the photoluminescence (PL) of ZnSxTe1-x mixed crystals grown by MBE, covering the entire composition range (0 less than or equal to x<1), were investigated. The results of Raman studies show that the phonons in ZnSxTe1-x mixed crystals display two-mode behavior. In addition, photoluminescence spectra obtained in backscattering and edge-emission geometries, reflection spectra and the temperature dependence of the photoluminescence of ZnSxTe1-x from 10 to 300K were employed to find out the origins of PL emissions in ZnSxTe1-x with different x values, The results indicate that the emission bands, for the samples with small x values, can be related to the band gap transitions or a shallow level emission center, while for the samples with large x values, they are designated to strong radiative recombinations of Te isoelectronic centers.

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We have investigated the Raman scattering and the photoluminescence (PL) of ZnSxTe1-x mixed crystals grown by MBE, covering the entire composition range (0 less than or equal to x < 1). The results of Raman studies show that the ZnSxTe1-x mixed crystals display two-mode behaviour. In addition, photoluminescence spectra obtained in backscattering and edge-emission geometries, reflectivity spectra and the: temperature dependence of the photoluminescence of ZnSxTe1-x have been employed to find out the origin of PL emissions in ZnSxTe1-x with different x values. The results indicate that emission bands, for the samples with small x values, can be related to the band gap transitions or a shallow-level emission centre, while as x approaches 1, they are designated to strong radiative recombination of Te isoelectronic centres (IECs).

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Two biosensors for fermentation process control have been introduced, which were developed in our lab recently. One is an enzyme electrode-based on-line monitoring system for glutamate fermentation process control and the other is an H+-ISFET-based ENFET for penicillin G fermentation process control.

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从芽抱杆菌属、酵母属二十株菌中筛选到一株优良伴生菌B529,与新选育出的产酸菌V6构成一新菌系B529-V6。新菌系B529-V6表现出了较强的高浓度L-山梨糖耐受能力、较高的底物代谢速率和较高的2-KGA转化能力,在8%山梨糖浓度的发酵培养基中培养48h,糖酸转化率较对照菌系提高了5.94%;山梨糖浓度提高至10%,其生长代谢受影响程度较小,且能不同程度地利用葡萄糖和山梨醇为底物,合成维生素C前体-2-酮基-L-古龙酸(2-KGA),其发酵产物2-KGA经反相高效液相色谱分析其质量符合工业化生产要求。对新菌系生长代谢规律及调控进行了研究,4M3罐和300M3罐发酵实验表明:种子培养基的碳源、葡萄糖/山梨糖浓度比、氮源、生长因子、接种种液质量及环境因子,均可影响新菌系的生长代谢。4M3罐发酵,新菌系具有周期短、糖酸转化率高等特点,连续4批发酵平均转化率较对照菌系提高10.18%,周期缩短23.7%。在300M3罐发酵试运行期间,新菌系糖酸转化率达到90.10%,较原生产菌系提高3.95%,发酵周期平均缩短1.3小时,显示出了较高的应用价值,在东北制药总厂进行了推广应用。研究了新产酸菌V6的基本生物学特性,分析了GC moL%含量,165rDNA同源性,鉴定其在系统发育学上应归入Ketoguloigenium 属,暂命名为Ketogulonigenium sp.V6。选用限制性内切酶Hind IH对新产酸菌V6染色体DNA进行了部分消化,应用载体pGEM-3zf(+)构建了v6的基因文库。结合阳性转化子在以L-山梨糖为唯一碳源培养基上的生长特性,利用PCR技术从该基因文库中,筛选到一株含有L-山梨糖还原酶(sR)基因的阳性克隆,并利用pET-32a(+)表达载体,实现了sR酶基因在大肠杆菌AD494(DE3)中的表达。SDS-PAGE电泳分析测定SR融合蛋白分子量大约在65kD左右,除去硫氧化还原蛋白、S-Tag和His-Tag蛋白,可推测出天然sR酶蛋白分子量约53 kD左右,与从SR基因推测出的分子量大小相符。另外,SR酶学特性研究表明,还原型辅酶II(NADPH)是sR酶蛋白的最适电子供体,其最适反应pH为7.0,pH6.5时保持稳定,酶活力较高;最适反应温度为50 ℃,30 ℃时热稳定性较好;lmM的Cu~(2+),Fe~(3+)和Mn~(2+)对该酶活抑制作用较大。