Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane

A highly catalytic activity microperoxidase-11 (MP-11) biosensor for H2O2 was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (DDAB) lipid membrane. The enzyme electrode thus obtained responded to H2O2 without electron mediator or promoter, at a potential of +0.10 V versus Ag \ AgCl. A linear calibration curve is obtained over the range from 2.0 x 10(-5) to 2.4 x 10(-3) M. The biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8 x 10(-7) M (S/N = 3) Providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors.

Behavioral responses of tilapia (Oreochromis niloticus) to acute fluctuations in dissolved oxygen levels as monitored by computer vision

Behavioral and ventilatory parameters have the possibility of predicting the stress state of fish in vivo and in situ. This paper presents a new image-processing algorithm for quantifying the average swimming speed of a fish school in an aquarium. This method is based on the alteration in projected area caused by the movement of individual fish during frame sequences captured at given time intervals. The image enhancement method increases the contrast between fish and background, and is thus suitable for use in turbid aquaculture water. Behavioral parameters (swimming activity and distribution parameters) and changes in ventilation frequency (VF) of tilapia (Oreochromis niloticus) responded to acute fluctuations in dissolved oxygen (DO) which were monitored continuously in the course of normoxia, falling DO level, maintenance of hypoxia (three levels of 1.5, 0.8 and 0.3 mg l(-1)) and subsequent recovery to normoxia. These parameters responded sensitively to acute variations in DO level; they displayed significant changes (P < 0.05) during severe hypoxia (0.8 and 0.3 mg l(-1) level) compared with normoxic condition, but there was no significant difference under conditions of mild hypoxia (1.5 mg l(-1) level). There was no significant difference in VF between two levels of severe hypoxia 0.8 and 0.3 mg l(-1) level during the low DO condition. The activity and distribution parameters displayed distinguishable differences between the 0.8 and 0.3 mg l(-1) levels. The behavioral parameters are thus capable of distinguishing between different degrees of severe hypoxia, though there were relatively large fluctuations. (c) 2006 Elsevier B.V. All rights reserved.

A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences

"Da-Huang" (Radix et Rhizoma Rhei, medicinal rhubarb), a famous and important Traditional Chinese Medicine, has often been confused with the adulterant species in the same genus, Rheum. Through sequencing the trnL (UAA)/trnF (GAA) regions of chloroplast DNA of thirteen species of Rheum (three medicinal rhubarb species and ten adulterant ones), a molecular marker of the medicinal species was found. A pair of PCR primers based on the sequences, was thus designed, which amplified a highly specific DNA fragment in medicinal rhubarb exclusively, and absent in the adulterants at all under an optimized PCR condition.

Robust Learning Control for Manipulators with Uncertainty Disturbances Based on Internal Model

In this paper, a disturbance controller is designed for making robotic system behave as a decoupled linear system according to the concept of internal model. Based on the linear system, the paper presents an iterative learning control algorithm to robotic manipulators. A sufficient condition for convergence is provided. The selection of parameter values of the algorithm is simple and easy to meet the convergence condition. The simulation results demonstrate the effectiveness of the algorithm..

龙滩工程左岸边坡监测资料分析与稳定性评价研究

On the basis of the geological analysis and rock mass toppling deformation and failure mechanism analysis of Longtan engineering left bank slope, the synthetic space-time analysis and influence factors analysis on the surface monitoring data and deep rock mass monitoring data of B-zone of left bank slope are carried on. At the same time, based on the monitoring data analysis in conjunction with the predecessor's mechanics analysis results, the deformation state of B-zone of the left bank slope is discussed and its stability is synthetically evaluated. The detailed research contents and results are as following: According to the monitoring drill histogram analysis of Longtan engineering left bank slope, numerical simulation analysis and model experimentation analysis of bedded counter-inclined steep slope, a new type of toppling deformation and failure mode is proposed, that is "up-slope warping". Then the deformation and failure mode of bedded counter-inclined steep slope is summarized as "down-slope toppling" type, "up-slope warping" type and "complex fold" type. On the basis of synthetic space-time analysis to surface monitoring data and deep rock mass deformation monitoring data of B-zone of Longtan left bank slope;, we can get the conclusion that there exists potential instability rock mass over 520m altitude, especially over 560m altitude of slope B, and the rock mass of around strong-weathering line or creep rock mass breaking band controls the deformation of the whole slope. 1. According to the synthetic space-time analysis and influence factors analysis to the surface monitoring data of B-zone of Longtan left bank slope, a dynamical index, accumulative total acceleration index, which is used to analyze the influence factors of slope surface deformation, is raised. The principle and method of accumulative acceleration index are explained, and the index can be used for the influence factors analysis of the similar slope. 2. Summarize the results of geologic analysis, monitoring analysis and mechanics analysis, the following conclusion can be gotten: the stability of B-zone of the slope is basically good. However, on the condition of drainage and slope toe loading engineering, there is still some creep deformation in the rock mass over 520m altitude, especially over 560m altitude. So, better measures of the monitoring and timely maintenance of the drainage system are suggested in the paper.

基于GIS的高速公路养护管理信息系统的设计与开发

China is experiencing a rapid development of highway ever since 1990s. By the end of 2004, the total length of the highway summed up to 33 thousand kilometers, ranking 2n in the world. After the open of highway, the accumulation of time and traffic causes the decrease of its capability. To ensure its good quality, security and operation functions, we should take some reasonable measures to maintain it periodically. At present, a big problem is that the traditional maintain measures can no longer meet the increasing requirements. Due to the characters of highway, the relationship of various maintenance data and geographic positions is even closer than any others. If we wan to improve the quality and efficiency of the maintenance work, particularly when there is need for decision-making, a great number of data that is related to geographic positions are absolutely necessary. Evidently, Geographical Information System (GIS) has incomparably advantages in dealing with these spatial information. As a result, a management system for highway maintenance work based on GIS became inevitable for the development of the maintenance of highway. The purpose of this paper is to establish a management system for highway maintenance work base on Geographical Information System (GIS), Global Positioning System (GPS) and spatial database, to manage all kinds of problems encountered in the work, and to provide support on information and methods. My study mainly includes: (1) Analysis on the current status of the maintenance and management work; overview on the history of domestic and international highway maintenance management systems; identifying the necessity and importance for establishing a management system for highway maintenance work based on GIS. (2) Based on the requirement analysis, I proposed a general design for this management system, and discussed the objective, design principles, framework, systematical structure and function design. (3) Outdoor data collection is not only a prime way to understand the current situation of the road, but also an important method for data update after the system is put into use. This paper also intends to establish a set of plan to collect data efficiently and precisely which is based on GIS and GPS technologies. (4) The maintenance management database is a supporting platform for various maintenance decision-makings. Such decisions need the support of a great amount of data, which would cause other problems, such as the diversity of the data source, difference of data formats. This paper also discussed how to deal with these problems and establish such a database. (5) To propose an approach to assess the condition of pavement, based on GIS and related maintenance models. Among all the maintenance models, the two for assessing and forecasting pavement condition are the most important and mature. This paper also analyzed these two models and introduced them in terms of the integration of models. (6) This paper took the Guangshen Highway for example, explaining how to realize a GIS for management of highway maintenance work.

Effect of pore ring number of zeolites on catalytic performance of Mo/Zeolite in methane aromatization under non-oxygen condition

The catalytic behavior of Mo-based zeolite catalysts with different pore structure and size, particularly with 8 membered ring ( M R), 10 M R, coexisted 10 and 12 M R, and 12 M R, was studied in methane aromatization under the conditions of SV=1500 ml/(g.h), p=0.1 MPa and T = 973 K. It was found that the catalytic performance is correlated with the pore structure of the zeolite supports. The zeolites that possess 10 MR or 10 and 12 MR pore structure with a pore diameter equal to or slightly larger than the dynamic diameter of benzene molecule, such as ZSM-5, ZSM-11, ZRP-1 and MCM-22, are fine supports. Among the tested zeolite supports, MCM-22 exhibits the highest activity and selectivity for benzene. A methane conversion of 10.5% with benzene selectivity of 80% was achieved over Mo/MCM-22 catalyst. The Mo/ERS-7 catalyst with 8 MR (0.45 nm) does not show any activity in methane dehydro-aromatization, while Mo/JQX-1 and Mo/SBA-15 catalysts with 12 MR pore exhibit little activity in the reaction. It can be concluded that the zeolites with 10 MR pore or coexisted 10 and 12 MR, having pore size equal to or slightly larger than the dynamic diameter of benzene molecule, are fine supports for methane activation and aromatization.