The Homology and Relationship of Human (Homo sapiens) Chromosomes 1, 19 and Dusky Langur (Trachypithacus obscurus) Chromosomes 6, 8 Demonstrated with Chromosome Painting

http://www.jstage.jst.go.jp/

Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory

Kallikrein 8 (KLK8) is a serine protease functioning in the central nervous system, and essential in many aspects of neuronal activities. Sequence comparison and gene expression analysis among diverse primate species identified a human-specific splice for

Anti Human Immunodeficiency Virus Type 1 (HIV-1) Agents 4. Discovery of 5,5 '-(p-Phenylenebisazo)-8-hydroxyquinoline Sulfonates as New HIV-1 Inhibitors in Vitro

To search for compounds with superior anti-human immunodeficiency virus type 1 (HIV-1) activity, ten 5,5'-(p-phenylenebisazo)-8-hydroxyquinoline sulfonates (4a-j) were synthesized and preliminarily evaluated as HIV-1 inhibitors in vitro for the first time. Some compounds demonstrated anti-HIV-1 activity, especially 5,5'-(p-phenylenebisazo)-8-hydroxyquinoline p-ethylbenzenesulfonate (4g) and 5,5'-(p-phenylenebisazo)-8-hydroxyquinoline p-chlorobenzenesulfonate (41) showed the more potent anti-HIV-1 activity with 50% effective concentration (EC50) values of 2.59 and 4.01 mu g/ml, and therapeutic index (TI) values of 31.77 and 24.51, respectively.

云南8种树蛙的染色体组型比较研究

报道了云南8种树蛙的染色体组型, 并对目前所已知的树蛙种类的染色体组型进行了比较。

异丙酚对大鼠海马区突触传递可塑性的影响

目的研究异丙酚对海马区突触传递和可塑性的影响。方法断头分离大 鼠海马半脑, 制备加阿厚度海马脑片。张脑片分为六组。脂肪乳剂组和异丙酚组的脑片以印防 己毒素预孵而, 然后加人川脂肪乳剂或异丙酚相当于拌, 观察对兴奋性突触后电流 的影响。月旨肪乳剂长时程增强】」下组、脂肪乳剂长时程抑制组、异丙酚功下组、异丙酚 组的脑片以川脂肪乳剂或异丙酚相当于脚预孵而, 给予高频刺激或低频 刺激, 记录或的发生情况。结果脂肪乳剂对无影响脚异 丙酚使细胞下降至基础值的尸, 使细胞玲上升至基础值的 。脂肪乳剂组给予邓后玲值为基础值的, 脂肪乳剂汀〕组给 予⋯乃后值为基础值的异丙酚组给予后, 可以产生但不能维 持, 后值为基础值的, 异丙酚几组给予后值为基础值的 , 明显低于脂肪乳剂组尸。结论异丙酚对大鼠海马区突触传递 具有双重影响, 出现抑制和兴奋两种效果异丙酚损害大鼠海马区锥体神经元的维持而易 化。 【关键

Sequence context analysis of 8.2 million single nucleotide polymorphisms in the human genome

We analyzed n-mers (n=3-8) in the local environment of 8,249,446 human SNPs and compared their distribution with that in the genome reference sequences. The results revealed that the short sequences, which contained at least one CpG dinucleotide, occurred

污水中8种雌激素化合物的定量测定

采用固相萃取-气相色谱-质谱法(GC-MS)测定污水中辛酚(OP)、壬酚(NP)、双酚A(BPA)、己烯雌酚(DES)、雌酮(E1)、17β-雌二醇(E2)、17α-乙炔雌二醇(EE2)和雌三醇(E3)8种具有雌激素活性的化合物。被测组分的加标回收率为(65.4±4.0)%～(110.0±4.5)%,检出限为1.0～7.5ng/L(相对标准偏差为5.2%～15.6%)。经检测,武汉某城市污水处理厂进水中的目标化合物(除EE2外)浓度为6.5～8954.9ng/L;除EE2和E3外,出水中的浓度为3.2～2

~(14)C标记1,2,7,8-TCDD在鲤体内分布及代谢的初步研究

利用14C标记1,2,7,8-四氯代二苯并二噁英(1,2,7,8Tetrachloro[U—14C]dibenzodioxin,14C1,2,7,8TCDD)初步研究了其在鲤体内的分布和代谢规律。14C1,2,7,8-TCDD溶解于丙酮/植物油中,腹腔暴露。暴露1、2、4、8、12d后取样,肝脏、胆汁、腹腔脂肪等消化制样后用液闪仪测量放射性活度。肝脏和胆汁内的放射性活度同步变化,都是第8d达到峰值后下降。腹腔脂肪内1—2d放射性明显高于随后取样的样品。肝和腹腔脂肪的分布量之比呈现“S”型变化趋势。暴露4d

2,3,7,8-四氯代二苯并二英(TCDD)和苯并芘(B[a]P)对原代培养鲫鱼肝细胞中卵黄蛋白原诱导的影响

通过测定原代培养鲫鱼(Carassius auratus)肝细胞中雌激素受体所介导的卵黄蛋白原(Vtg)生成以及芳香烃受体所介导的CYP1A1基因转录水平的变化, 建立了一种类雌激素体外实验模型. 实验结果表明, Vtg和Vtg mRNA的表达与己烯雌酚(DES)之间均有很好的剂量-效应关系, Vtg和Vtg mRNA均可作为指示类雌激素毒性的生物标志物. TCDD, B[a]P可显著抑制鱼肝细胞中DES诱导的Vtg和Vtg mRNA的表达, 呈明显的抗雌激素效应, 并同时激活了CYP1A1 基因的表达;

长江三峡库区鱼类资源现状的研究

1997— 2 0 0 0年在长江三峡库区的巴南和万州江段共监测 1 2 92船次 ,统计渔获物40 96.38kg,生物学测定 2 4 64尾 ,共采集鱼类 30种 ,分属 8科 2 4属。目前库区渔获物组成和结构与 2 0世纪 70年代相比发生了较大变化 ,白甲鱼、中华倒刺、岩原鲤等在渔获物中比例已经很少 ,铜鱼资源也明显下降 ,、鳡、等在渔获物中已基本消失 ,而南方鲇、鲤、黄颡鱼等比例相对上升 ,渔获物中高龄鱼个体减少 ,低龄鱼及幼鱼个体比重增加。与 70年代资料相比 ,三峡库区鱼类资源已呈衰

2,3,7,8-TCDD对稀有鮈鲫幼体超微结构影响的观察

国家自然科学基金29607004

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

In this study, an IL-8 homologue has been cloned and identified from a reptile, Chinese soft-shelled turtle for the first time. The full-length cDNA of turtle IL-8 was 1188 bp and contained a 312 bp open reading frame (ORF) coding for a protein of 104 amino acids. The chemokine CXC domain, which contained Glu-Leu-Arg (ELR) motif and four cysteine residues, was well conserved in turtle IL-8. The 4924 bp genomic DNA of turtle IL-8 contained four exons and three introns. Phylogenetic analysis showed that the amino acid sequence of turtle IL-8 clustered together with birds. RT-PCR analysis showed that turtle IL-8 mRNA was constitutively expressed liver, spleen, kidney, heart, blood and intestine tissues of control turtles. Real-time quantitative PCR analysis further indicated that the turtle IL-8 mRNA expression was apparent in various tissues at 8 h and up-regulated significantly during 8 h-7 d after Aeromonas hydrophila infection. The present studies will help us to understand the evolution of IL-8 molecule and the inflammatory response mechanism in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.

Elevated levels of urinary 8-hydroxy-2'-deoxyguanosine in male electrical and electronic equipment dismantling workers exposed to high concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans, polybrominated diphenyl ethers, and polychlorinated biphenyls

To investigate the occupational exposure levels to polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polybrominated diphenyl ethers (PBDEs), and polychlorinated biphenyls (PCBs), indoor dust (n = 3) in workshops and hair samples from male workers (n = 64) were collected at two electrical and electronic equipment waste (E-waste) dismantling factories located in the LQ area in east China in July 11-13, 2006. Pre- and postworkshift urines (64 of each) were also collected from the workers to study oxidative damage to DNA using 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker. The concentrations of PCDD/Fs, PCDD/F-WHO-TEQs, PBDEs, PCBs and PCB-WHO-TEQs were (50.0 +/- 8.1) x 10(3), 724.1 +/- 249.6, (27.5 +/- 5.8) x 10(6), (1.6 +/- 0.4) x 10(9), (26.2 +/- 3.0) x 10(3) pg/g dry weight (dw) in dust, and (2.6 +/- 0.6) x 10(3), 42.4 +/- 9.3, (870.8 +/- 205.4) x 10(3), (1.6 +/- 0.2) x 10(6), 41.5 +/- 5.5 pg/g dw in hair, respectively. The homologue and congener profiles in the samples demonstrated that high concentrations of PCDD/Fs, PBDEs, and PCBs were originated from open burning of E-waste. The 8-OHdG levels were detected at 6.40 +/- 1.64 mu mol/mol creatinine in preworkshift urines. However, the levels significantly increased to 24.55 +/- 5.96 mu mol/mol creatinine in postworkshift urines (p < 0.05). Then, it is concluded that there is a high cancer risk originated from oxidative stress indicated by the elevated 8-OHdG levels in the E-waste dismantling workers exposed to high concentrations of PCDD/Fs, PBDEs, and PCBs.