28 resultados para Trigger
Resumo:
Yields, correlation shapes, and mean transverse momenta p(T) of charged particles associated with intermediate-to high-p(T) trigger particles (2.5 < p(T) < 10 GeV/c) in d + Au and Au + Au collisions at root s(NN) = 200 GeV are presented. For associated particles at higher p(T) greater than or similar to 2.5 GeV/c, narrow correlation peaks are seen in d + Au and Au + Au, indicating that the main production mechanism is jet fragmentation. At lower associated particle pT < 2 GeV/c, a large enhancement of the near- (Delta phi similar to 0) and away-side (Delta phi similar to pi) associated yields is found, together with a strong broadening of the away-side azimuthal distributions in Au + Au collisions compared to d + Au measurements, suggesting that other particle production mechanisms play a role. This is further supported by the observed significant softening of the away-side associated particle yield distribution at Delta phi similar to pi in central Au + Au collisions.
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本论文描述了多路信号处理与QDC(电荷数字转换器)门信号产生逻辑插件的研制过程。该逻辑插件的逻辑电路和模拟电路都经过了精心的设计和调试。核物理研究沿着激发能、核自旋及核同位旋三个自由度深入开展,产生了核物理研究中许多新的前沿领域。随着入射能量的增加,开放的反应道增多,多重探测器投入使用,使核物理实验的数据参数已经从几十路上升到上千路,即需要电子学工作者研制和开发一些高速触发、高密度、高稳定性的电子学硬件系统。本论文描述的多路信号处理与QDC门信号产生逻辑插件,主要用于产生QDC的开门信号。该电路系统早十六路并行、独立的高速放大单元电路、高速比较单元电路、延迟和脉宽成形单元电路、逻辑信号扇出单元电路、触发指示单元电路及多路模拟信号求和单元电路构成。该逻辑插件可接收≥ 20mv的小辐度输入信号而产生32路标准ECL(高电平-0.8V,低电平-1.6V)互补输出逻辑信号。输出ECL逻辑信号用于产生QDC的门控信号。该插件的多路模拟信号求和单元电路对每路输入信号可产生幅度为50mv的输出信号。该输出信号可用于多重符衙事件的鉴别和产生trigger触发信号。总的来说,多路辐度信号处理与QDC门信号产生逻辑插件不仅适用于重离子核物理实验中产生QDC开门信号,也可用于其它核物理实验的多重符合事件的鉴别。该插件的研制成功为多参数获取系统提供了一个良发的前端硬件设备。
Resumo:
In this work, we illustrate a simple chelation-based strategy to trigger DNA release from DNA-incorporated multilayer films, which were fabricated through the layer-by-layer (LbL) assembly of DNA and inorganic zirconium (IV) ion (Zr4+). After being incubated in several kinds of chelator solutions, the DNA multilayer films disassembled and released the incorporated DNA. This was most probably due to the cleavage of coordination/electrostatic interactions between Zr4+ and phosphate groups of DNA. Surface plasmon resonance (SPR), UV-vis spectrometry and atomic force microscopy (AFM) were used to characterize the assembly and the disassembly of the films.
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By using AuNP-modified homo-adenine DNA conjugate as a model system, simple colorimetric and resonance Rayleigh scattering assays have been developed for screening small molecules that trigger the formation of the non-Watson-Crick homo-adenine duplexes. The assay presented here is more simplified in format as it involves only one type of ssDNA modified Au-NP, and can be easily adapted to high-throughput screening.
Resumo:
MEP is a member of thioester-containing protein (TEP) family found in Zhikong scallop Chlamys farreri and is involved in innate immunity against invading microbes. In the present study, the genomic DNA of CfTEP was cloned and characterized. The genomic DNA sequence of CfTEP consisted of 40 exons and 39 introns spanning 35 kb with all exon-intron junction sequences agreeing with the GT/AG consensus. The genomic organization of CfTEP was similar to human and mouse 0 rather than ciona C3-1 and Drosophila dTEP2. By RT-PCR technique, seven different cDNA variants of CfTEP (designated as CfTEP-A-CfTEP-G) were cloned from scallop gonad. CfTEP-A-CfTEP-F were produced by alternative splicing of six mutually exclusive exons (exons 19-24), respectively, which encoded the highly variable central region. While in CfTEP-G, the deletion of all the six exons introduced a new translation stop site and might trigger nonsense mediated decay (NMD). The mRNA expression and the proportion of the seven CfTEP variant transcripts were examined in the gonad of scallops after bacterial challenge. The fragments containing the highly variable central region of UTEP were amplified by RT-PCR and a 100 positive clones were sequenced randomly. The expression profiles of the seven MEP variants were different and displayed the sex and bacteria dependent manner. In the blank, sea water and Listonella anguillarum challenged subgroups of male scallops, all the transcripts detected were CfTEP-G isoform. In the Micrococcus luteus challenged subgroup, the isoforms expressed and their proportions were CfTEP-F (54%), CfTEP-B (23%), CfTEP-A (10%), CfTEP-C (7%) and CfTEP-E (6%). However, in the gonad of female scallops, only CfTEP-A were found in blank and sea water challenged subgroups. After L anguillarum or M. luteus challenge, four and five isoforms were detected, respectively, with CfTEP-F isoform being the most one in the both subgroups. These results suggested that the evolution of TEP genes was very complex, and that the diverse CfTEP transcripts generated by alternative splicing played an important role as pattern recognition receptors in the innate immune defense of scallops. (C) 2009 Elsevier Ltd. All rights reserved.
Resumo:
Porphyra yezoensis Ueda (Rhodophyta) is a seaweed of economic importance with a typical dimorphic life cycle consisting of a leafy gametophyte and a filamentous sporophyte. Recently, it has been recognized as a model system for fundamental and applied studies in marine biological sciences. Conchospore, a major spore linking the two distinct multicellular phases in the life cycle, is most widely used in the breeding of P. yezoensis. In this paper, the early-stage development of conchospore, including the attachment and the cell wall formation, was studied with fluorescent reagents staining and Scanning Electron Microscopy detection. Results displayed: (I) the cell wall began to be generated after culturing for 4 h in the attached conchospores; (2) the initially released conchospores were plastids with some filmy, amorphous substance on the surface, and they attached to the fibers firmly via the actively secreted mucilaginous substances after their touch to the fibers; (3) cellulase and pectolase prohibited the attachment of conchospores in the different ways; and (4) only attached conchospores generated cell walls and developed normally, while the suspending ones could not. It indicated that the cellulose played crucial roles in the permanent attachment as the pectin did in the initial attachment. The conchospore attachment seemed to trigger the cell wall formation and the further development. Affects of light on the development of conchospores were also discussed. The results showed that high intensity (200 mu mol.m(-2).s(-1)) and long-wave (>= 580 nm) light facilitated the division rate of conchospores. (C) 2008 Elsevier B.V. All rights reserved.
Resumo:
Field-collected tetrasporophytes of Palmaria palmata were tumbled in 300-L outdoor tanks from January to August at ambient daylength or in a constant short-day (SD) regime (8 h light per day), both at 10 or 15 degrees C. Tetrasporangia were massively induced after 2.5 months under SD conditions at 10 degrees C and completely lacking at 15 degrees C, both under SD or ambient daylength conditions, with a few tetrasporangia present at 10 degrees C and ambient daylength. Elongation rates of tagged tetrasporophytic thalli peaked from March to April in all four conditions, when the biomass densities in the outdoor tanks were close to 2.5 kg fresh weight m(-2). Under all four conditions, juvenile proliferations started to appear in June from the margins of the old fronds, and attained approximately 1 cm in length by the end of July. Approximately 80% of the tetraspores were released during the first three dark phases in a light/dark regime, and the remaining 20% during the light phases. A minimum of 10 min darkness was observed to trigger spore release. White light inhibited tetraspore release, while a similar number of spores were released in continuous red light or in the light/dark regime, although with no significant differences of spore release during subjective days and nights. Sporelings were successfully derived from the released tetraspores for mass propagation of the male gametophyte in 2000-L outdoor tanks in a greenhouse. Mass production of male gametophytic sporelings of P. palmata was completed two times by SD induction of tetrasporangia at 10 degrees C, release of spores in darkness and culturing the sporelings until they were ready to be propagated vegetatively in greenhouse tanks. One experiment lasted from January to October 2001, with spore release in June, and the second from September to April 2003, with spore release in January. These results may support the development of sustainable, year-round Palmaria farming. (c) 2005 Elsevier B.V. All rights reserved.
Resumo:
Quantitative studies on the evolution and dynamics of the deepwater area of Pearl River Mouth basin (PRMB) were carried out based on the latest geological and seismic data. The study area is generally in an extensional state during the Cenozoic. The major extension happened in the earlier syn-rift stages before 23 Ma and the extension after 23 Ma is negligible. Two rapid subsidence periods, 32-23 Ma and 5.3-2.6 Ma, are identified, which are related to the abrupt heat decay during margin breakup and the collision between the Philippine Sea plate and the Eurasian plate, respectively. The strongest crustal thinning in the Baiyun (sic) sag may trigger the syn-rift volcanism along the weak faulted belt around the sag. The Cenozoic tectonic evolution of the study area could be divided into five stages: rifting (similar to 50-40 Ma), rift-drift transition (similar to 40-32 Ma), early post-breakup (similar to 32-23 Ma), thermal subsidence (similar to 23-5.3 Ma) and neotectonic movement (similar to 5.3-0 Ma).
Resumo:
研究区位于郯庐断裂中段与济阳坳陷的构造结合部,区内走滑构造广泛发育,主要的走滑断裂有7条,分别是郯庐断裂带的东西两支、垦东断层、孤东断层、长堤断层、埕东断层和发育于垦东凸起中部的浅层走滑构造带。走滑构造带与油气富集带有着明显的对应关系。 通过对研究区内二维、三维地震测线和平面构造图的精细解释和分析,分别揭示了各走滑断裂在平面、剖面和三维空间上的构造形态。根据走滑断裂及其伴生构造的平面和剖面上的几何学特征,将研究区内的走滑断裂划分为三种类型:成熟型走滑断裂、隐伏型走滑断裂、不连续型的走滑断裂。 从理论模式研究入手,推导了拉分盆地中盆地的走滑速率与沉降速率之间的关系,证实了走滑速率同盆地的几何形状参数、最大沉降深度和盆地的沉降速率存在着稳定的数值关系。通过对莱州湾地区潍北凹陷基底沉降历史的分析,建立了潍北凹陷沉降速率与郯庐断裂中段走滑速率之间的经验关系式,进而求出郯庐断裂中段新生代右行走滑位移量的大小为40km。 运用2DMove软件,对研究区内四条典型剖面进行构造复原,计算出了各条剖面每个时期的伸展参数,对研究区构造活动强度进行了定量分析,揭示了研究区的构造演化规律。通过运用Ansys软件进行有限元模拟,恢复了晚白垩世晚期-古近纪早期研究区内的构造应力场和应变场,揭示了扭张作用是研究区内走滑断层开始走滑的主要原因。 通过上述分析,结合对究区内近几年勘探开发成功和失败的实例分析,全面探讨了走滑活动对于油气成藏“生”、“储”、“盖”、“圈”、“运”、“保”各因素的影响。
Resumo:
紫菜是一类海洋藻类研究的模式系统,具有重要的经济价值和理论研究意义。本研究基于紫菜的世代交替生活史,对其壳孢子萌发过程进行了研究,并对丝状孢子体与叶状配子体的世代差异进行了分析,主要包括以下三部分: 1) 对紫菜丝状孢子体与叶状配子体的连接枢纽——壳孢子的发育过程及其光影响进行了研究与讨论,发现壳孢子只有在附着后才能形成细胞壁并发育,说明壳孢子的附着是触发了壳孢子细胞壁的形成以及后期发育的开关,亦即附着是触发紫菜世代交替过程中配子体转录组表达的开关;纤维素酶和果胶酶均能抑制壳孢子附着,但影响机制各不相同,推测果胶质主要介导壳孢子的初始附着,而纤维素则与永久附着相关;波长≥580 nm的高强度(200 μmol•m-2•s-1)可见光有利于壳孢子早期发育。 2) 结合现有藻类数据,对坛紫菜丝状孢子体阶段11000 EST数据进行了大规模的生物信息学分析,结果首次发现坛紫菜丝状孢子体中可能存在PCK型C4光合固碳途径,并筛选出44条在紫菜孢子体中表达上调的代表基因。 3) 结合坛紫菜、条斑紫菜、海带和红毛菜,对红藻和褐藻等大型海藻孢子体与配子体阶段代表基因Rubisco的表达与羧化酶活性差异进行了研究分析,结果表明Rubisco的表达量和初始羧化酶活在其配子体中均显著高于其孢子体世代,即与藻体不同世代的相对复杂度无关,而与染色体倍性相关,说明Rubisco的世代差异极有可能与染色体倍性相连锁,因而可能是海藻世代交替过程中的重要功能基因。
Resumo:
Changes in photoperiod, ambient temperature and food availability trigger seasonal acclimatization in physiology and behavior of many animals. In the present study, seasonal adjustments in body mass and in several physiological, hormonal, and biochemical markers were examined in wild-captured plateau pikas (Ochotona curzoniae) from the Qinghai-Tibetan plateau. Our results showed that plateau pikas maintained a relatively constant body mass throughout the year and showed no seasonal changes in body fat mass and circulating levels of serum leptin. However, nonshivering thermogenesis, cytochrome c oxidase activity, and mitochondrial uncoupling protein 1 (UCP1) contents in brown adipose tissues were significantly enhanced in winter. Further, serum leptin levels were positively correlated with body mass and body fat mass while negatively correlated with UCP1 contents. Together, these data suggest that plateau pikas mainly depend on increasing thermogenic capacities, rather than decreasing body mass, to cope with cold, and leptin may play a potential role in their thermogenesis and body mass regulation.
Resumo:
The relationship between exogenous attention and endogenous attention is still unclear. We conclude the result of previous studies and our experiments by proposing a convergence hypothesis. A lot of evidences from ERP and fMRI proved the distinction between exogenous and endogenous attention. The distinction is fit for the complicated surroundings. Some studies found that exogenous and endogenous attention activated some common brain areas but if there’s any common function in these areas was still unclear. Previous studies failed to disentangle the relationship between these two mechanisms in detail, and most of the previous studies compared these two mechanisms separately. We used the cue-target paradigm, controlled the SOA and the cue validity to separate endogenous and exogenous attention. We try to figure out the relationship between endogenous and exogenous attention. We found that, in the exogenous attention condition, the amplitude of the P1 component was larger and the amplitude of N1 was smaller than that in the invalid trials. In the endogenous attention condition, the amplitudes of the P1 and N1 components in valid trials were both larger than that in the invalid trials. The difference wave of endogenous and exogenous attentions showed that at the beginning of their process endogenous and exogenous attentions were separate and then the process was going to converge. The stimuli appear in the visual field will activate the “external trigger” in exogenous attention processing, and then the signal will be transferred to next stage, in the endogenous attention processing, when the observer want to attend, the “inner trigger” will be activated and the signal will be transfered. The difference between “external trigger” and “inner trigger” in endogenous and exogenous attention was significant, so the difference at the beginning of the process was significant. When the signal was transferred to the next processing stage, the difference between the endogenous and exogenous attention became diminished, and it was a convergence process. We used the cue-target paradigm, let the central cue and peripheral cue appear in one trial, try to investigate the relationship between endogenous and exogenous attention under different perceptual load conditions. The result from of difference wave showed that the relationship between the two attentions was consistent with the convergence hypothesis. We also found that under different perceptual load conditions, the relationship between endogenous and exogenous attention was different. The perceptual load has influence on the “external trigger” and “inner trigger”. A cue-target paradigm was used to investigate the relationship between attention, age and perceptual load, we found that the function of visual attention was decrease in the old group, they need more attentional resource when they face the issue. The aging effect has influence on the “external trigger” and “inner trigger”. The effects of age and perceptual load were much higher in exogenous attention condition.
Resumo:
Zeigarnik effect refers to the enhanced memory performance for unfinished tasks and studies on insight using hemi-visual field presentation technology also find that after failing to solve an problem, hints to the problem are more effective received and lead to insight experience when presented to the left-visual field (Right hemisphere) than presented to the right-visual field, especial when the hints appeared with a delay. Thus, it seems that right hemisphere may play an important role in preserving information of unsolved problems and processing related cues. To further examine the finding above, we introduce an Chinese character chunking task to investigate the brain activities during the stage of failure to resolve problems and of hint presentation using Event-Related Potentials (ERP) and functional MRI technology. Our FMRI results found that bilateral BA10 showed more activation when seeing hints for unsolved problems and we proposed that it may reflect the processes of information to failure problems, howerver, there was no hemispheric difference. The ERP results after the effort to the problems showed that unsolved problems elicited a more positive P150 over the right frontal cortex while solved problems demonstrated a left hemispheric advantage of P150. When hints present, P2 amplitudes of hints were modulated by the status of problem only in the right hemisphere but not in the left hemisphere. Our results confirmed the hypothesis that failure to solve problems would trigger the perseverance processes in right hemisphere, which would make right hemisphere more sensitive to related information of failure problems.