华南地壳岩石弹性和变形性质的高温高压实验研究

The South continent of China lies to southeast of Eurasia block. It is an active area from the view of crust growth and continent spread and is a transition zone between continental crust and oceanic crust. The compressional wave velocities and anisotropies of typical crustal metamorphic rocks were determined at high temperature (up to 1000 ℃) and high pressure(up to 800MPa). The experimental results show that the velocities generally increase with pressure, and is unaffected by temperature up to around 550 ℃. But the velocities of all experimental samples start to drop above a temperature point. For an example, this greatly reduce the speed of wave propagation in amphibolite and serpentinite above 760 ℃ and above 550 ℃ respectively, which may be due to dehydrate of amphibole and serpentine. P-wave anisotropy coefficients of those rocks range from 2% to 10% at 800MPa and 500 ℃. The anisotropies decrease with increasing pressure at room temperature, but hardly change as function of temperature at constant 800MPa or 600MPa pressure. The average velocity of the six crustal rocks is 6.28km/s under the condition of 800MPa and 550 ℃, which is consistent with the result of deep seismic sounding data. Based on this experimental result, we deduce there may exist a lot of felsic granulites and amphibolites at the depth of 15-25km underground. With increasing temperature and pressure, the deformation behavior of the rocks undergoes from localized brittle fracture, semi-brittle deformation (cataclastic flow or semi-brittle faulting, semi-brittle flow) to homogeneous crystal-plastic flow. This transition is associated with mechanical behavior and micro-mechanism. It is very important to understanding earthquake source mechanics, the strength of the lithosphere and the style of deformation. The experiments were conducted at temperature of 600-1000 ℃, confining pressure of 500MPa, and stain rates of 10~(-4)-10~(-6) S~(-1). For fine-grained natural amphibolite, the results of experiments show that brittle faulting is major failure mode at temperature <600 ℃, but crystal-plastic deformation is dominate at temperature >800 ℃, and there is a transition with increasing temperature from sembrittle faulting to cataclastic flow and sembrittle flow at temperature of 670-750 ℃. For medium-grained natural Felsic granulite, the results of experiments show that brittle faulting is major failure mode at temperature <500 ℃, but crystal-plastic deformation is dominate at temperature >700 ℃, and there is a transition with increasing temperature from semibrittle faulting to cataclastic flow and sembrittle flow at temperature of 500-600 ℃.

Development of an in vitro multicellular tumor spheroid model using microencapsulation and its application in anticancer drug screening and testing

In this study, an in vitro multicellular tumor spheroid model was developed using microencapsulation, and the feasibility of using the microencapsulated. multicellular tumor spheroid (MMTS) to test the effect of chemotherapeutic drugs was investigated. Human MCF-7 breast cancer cells were encapsulated in alginate-poly-L-lysine-alginate (APA) microcapsules, and a single multicellular spheroid 150 mu m in diameter was formed in the microcapsule after 5 days of cultivation. The cell morphology, proliferation, and viability of the MMTS were characterized using phase contrast microscopy, BrdU-Iabeling, MTT stain, calcein AM/ED-2 stain, and H&E stain. It demonstrated that the MMTS was viable and that the proliferating cells were mainly localized to the periphery of the cell spheroid and the apoptotic cells were in the core. The MCF-7 MMTS was treated with mitomycin C (MC) at a concentration of 0.1, 1, or 10 times that of peak plasma concentration (ppc) for up to 72 h. The cytotoxicity was demonstrated. clearly by the reduction in cell spheroid size and the decrease in cell viability. The MMTS was further used to screen the anticancer effect of chemotherapeutic drugs, treated with MC, adriamycin (ADM) and 5-fluorouracil (5-FU) at concentrations of 0.1, 1, and 10 ppc for 24, 48, and 72 h. MCF-7 monolayer culture was used as control. Similar to monolayer culture, the cell viability of MMTS was reduced after treatment with anticancer drugs. However, the inhibition rate of cell viability in MMTS was much lower than that in monolayer culture. The MMTS was more resistant to anticancer drugs than monolayer culture. The inhibition rates of cell viability were 68.1%, 45.1%, and 46.8% in MMTS and 95.1%, 86.8%, and 91.6% in monolayer culture treated with MC, ADM, and 5-FU at 10 ppc for 72 h, respectively. MC showed the strongest cytotoxicity in both MMTS and monolayer, followed by 5-FU and ADM. It demonstrated that the MMTS has the potential to be a rapid and valid in vitro model to screen chemotherapeutic drugs with a feature to mimic in vivo three-dimensional (3-D) cell growth pattern.