Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp

White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV. In this study, a recombinant antibody library, as single-chain fragment variable (scFv) format, displayed on phage was constructed using mRNA from spleen cells of mice immunized with-full-length VP28 expressed in Escherichia coli. After several rounds of panning, six scFv antibodies specifically binding to the epitopes in the N-terminal, middle, and C-terminal regions of VP28, respectively, were isolated from the library. Using these scFv antibodies as tools, the epitopes in VP28 were located on the envelope of the virion by immuno-electron Microscopy, Neutralization assay with these antibodies in vitro suggested that these epitopes may not be the attachment site of WSSV to host cell receptor. This study provides a new way to investigate the structure and function of the envelope proteins of WSSV. (c) 2008 Published by Elsevier Inc.

Keyword spotting system based on biomimetic pattern recognition

The mandarin keyword spotting system was investigated, and a new approach was proposed based on the principle of homology continuity and point location analysis in high-dimensional space geometry theory which are both parts of biomimetic pattern recognition theory. This approach constructed a hyper-polyhedron with sample points in the training set and calculated the distance between each test point and the hyper-polyhedron. The classification resulted from the value of those distances. The approach was tested by a speech database which was created by ourselves. The performance was compared with the classic HMM approach and the results show that the new approach is much better than HMM approach when the training data is not sufficient.

1.78 μm Strained InGaAs-InGaAsP-InP Distributed Feedback Quantum Well Lasers

Ridge-waveguide distributed-feedback(DFB) lasers with highly strained InGaAs/InGaAsP active regions,emitting at 1.78 μm were fabricated by low pressure metal-organic vapor phase epitaxy(LP-MOVPE) and tested.The lasers exhibited threshold current of 33 mA for 900 μm long cavities at room temperature.A maximum light output power of 8 mW from one facet and an external differential quantum efficiency of 7% were also obtained.In oddition,the side mode suppression ratio (SMSR) is 27.5 dB.

Wavelength Tuning in Two-Section Distributed Bragg Reflector Laser by Selective Intermixing of InGaAp-InGaAsP Quantum Well Structure

The two-section tunable ridge waveguide distributed Bragg reflector (DBR) laser fabricated by the selective intermixing of an InGaAsP-InGaAsP quantum well structure is presented. The threshold current of the laser is 51mA. The tunable range of the laser is 4.6nm, and the side mode suppression ratio (SMSR) is 40dB.

铬硅化物的形成及其界面反应

于2010-11-23批量导入

低功率氩电弧加热发动机内传热与流动的数值模拟

<正>电弧加热发动机是通过阴极和阳极之间直流放电产生的电弧加热推进剂,产生的高温部分电离气体经喷管加速产生推力。国外早在上个世纪50年代就对电弧加热发动机开始研究,经过几十年努力,先后攻克了电极烧蚀、电弧稳定等一系列难题,终于研制成功千瓦量级的用于同步卫星位置保持和姿态调整的推进系统,并得到了广泛的商业应

镇江下蜀土剖面的化学风化强度与元素迁移特征

IEECAS SKLLQG

土壤水分与短期遮光对棉花光合及其气孔响应的影响

以陆地棉 (Gossypium hirsutum L.Zhongmain No.2 3)为供试材料 ,探讨了在充分供水-水分胁迫 -复水的处理过程中 ,短期不同遮光水平对棉花光合特性及其气孔响应的影响。结果表明 ,在水分处理过程中 ,所有不同遮光水平的棉花叶片对短期遮光具有相似的基本响应规律 :短期遮光使净光合速率迅速降低 ,气孔导度减少 ,但减少速率缓慢 ;遮阳网去掉后 ,叶片气孔重新开放速率和光合恢复被延迟。水分胁迫期间 ,所有遮光处理的水分利用效率均最高 ,但净光合速率均较低 ,光合及其气孔响应受到严重限制 ,遮光 75%受到的影响大于遮光 40 %的处理。复水后水分胁迫带来的残留影响仍然存在 ,结果使水分利用效率在整个水分处理过程中最低 ,遮光 75%的处理尤为明显。上述表明 ,土壤水分与短期遮光对棉花光合及其气孔响应的影响十分显著

论市场经济体制下自然资源的合理利用

论市场经济体制下自然资源的合理利用李法云，范志平（中科院沈阳应用生态研究所）一、计划经济体制下自然资源利用中存在的问题经济社会中的资源配置是指各种生产要素的优化组合，是自然资源与社会资源之间的协调；在计划经济体制下，由于没有形成标志资源稀缺性的合理价...