77 resultados para 332.17
Resumo:
该文报道了雀形目鶲科画眉亚科17种鸟类核型, 臂间倒位和小染色体缺失在该亚科进化中起重要作用. 在核型比较基础上结合古地理以及生态资料对该类群的起源和进化作了初步探讨。
Resumo:
选用实验室克隆的17个鳙鱼微卫星分子标记分析四川泸州和江西鄱阳湖的两个种群鳙鱼的遗传多样性及种质特性,计算和统计了杂合度、多态信息含量(PIC)、有效等位基因数、等位基因频率、遗传距离、遗传相似系数、Hardy-Weinberg平衡偏离指数等方面内容。结果表明:选择使用17个微卫星标记,其中有4个为单态标记,13个为多态标记。江西和四川鳙鱼群体每个微卫星位点的平均等位基因数分别为3.325及3.882,平均有效等位基因数分别为3.531及2.676,多态位点百分率分别为82.4及70.5,17个微卫星标记
Resumo:
<正> 日常生活中,B族的维生素是人们所熟悉的。例如B_1、B_2、B_4、B_6和B_(12)等,凡缺乏B族维生素就会使人们患各种维生素缺乏症。近年来国际上又发现了一种新的B族成员即B_(17),它的发现使人们充满了对癌症防治的信心,为防癌、抗癌药物增添了一朵奇葩。食品中有哪些含有B_(17),据记载有水果类、藻类、植物叶和鳖类 一、杏子:话还得从头说起,在南太平洋中有一个斐济岛国。该国气候终年如春,以盛产杏子
Resumo:
Present in the excrement of humans and animals, 17 beta-estradiol (E-2) has been detected in the aquatic environment in a range from several nanograms to several hundred nanograms per liter. In this study, the sensitivities of rare minnows during different life stages to E-2 at environmentally relevant (5, 25, and 100 ng l(-1)) and high (1000 ng l(-1)) concentrations were compared using vitellogenin (VTG) and gonad development as biomarkers under semistatic conditions. After 21 days of exposure, VTG concentrations in whole-body homogenates were analyzed; the results indicated that the lowest observed effective concentration for VTG induction was 25 ng l(-1) E-2 in the adult stage, but 100 ng l(-1) E-2 in the larval and juvenile stages. After exposure in the early life stage, the larval and juvenile fish were transferred to clean water until gonad maturation. No significant difference in VTG induction was found between the exposure and control groups in the adults. However, a markedly increased proportion of females and appearance of hermaphrodism were observed in the juvenile-stage group exposed to 25 ng l(-1) E-2. These results showed that VTG induction in the adult stage is more sensitive than in larval and juvenile stages following exposure to E-2. The juvenile stage may be the critical period of gonad development. Sex ratio could be a sensitive biomarker indicating exposure to xenoestrogens in early-life-stage subchronic exposure tests. The results of this study provide useful information for selecting sensitive biomarkers properly in aquatic toxicology testing.
Resumo:
This study investigates the ozonation of 17 alpha-ethinylestradiol (EE2) in aqueous solution. The affecting factors on the degradation of EE2 were studied and described in details, such as initial EE2 concentration, initial pH value and ozone concentration. In addition, some parameters such as pH. electrical conductivity, mineralization efficiency and degradation products were monitored during the process. The mineralization efficiency of EE2 could reach 53.9%. During the ozonation process the rapid decrease of pH and the sharp increase of electrical conductivity indicated the fort-nation of acidic by-products, small fragments and ions which were confirmed by high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GUMS) analysis. Results showed that there were intermediate products of smaller molecule with higher polarity produced during the course of EE2 degradation. Then a possible reaction pathway for EE2 degradation involving all intermediates detected is proposed. During the ozonation process EE2 was first oxidized into hydroxyl-semiquinone isomers which were subsequently degraded into low molecular weight compounds such as oxalic acid, malonate, glutarate, and so on. Furthermore. these organic acids are easily oxidized by ozone into carbon dioxide (CO2). This work shows that ozonation process is promising for the removal of EE2. The results can provide some useful information for the potential treatment of EE2 by ozonation in aqueous solution. (c) 2005 Elsevier B.V. All rights reserved.
Resumo:
Undoped Ga-Sb samples were investigated by positron lifetime spectroscopy (PAS) and the coincident Doppler broadening (CDB) technique. PAS measurement indicated that there were monovacancy-type defects in undoped Ga-Sb samples, which were identified to be predominantly Ca vacancy (V-Ga) related defects by combining the CDB measurements. After annealing of these samples at 520 C, positron shallow trapping have been observed and should be due to Ga-Sb defects. Undoped Ga-Sb is intrinsically p-type having a residual carrier density of 10(16)-10(17) cm(-3). And the Ga-Sb antisite defects are stable in the (0), (1-) and (2-) charge states and act as a double acceptor. Thus, we infer that Ga-Sb antisite defects are the acceptor contributing to the p-type conduction for undoped samples. (C) 2004 Elsevier B.V All rights reserved.
Resumo:
This paper reports that lnAs/In0.53Ga0.47As/AlAs resonant tunnelling diodes have been grown on InP substrates by molecular beam epitaxy. Peak to valley current ratio of these devices is 17 at 300K. A peak current density of 3kA/cm(2) has been obtained for diodes with AlAs barriers of ten monolayers, and an In0.53Ga0.47As well of eight monolayers with four monolayers of InAs insert layer. The effects of growth interruption for smoothing potential barrier interfaces have been investigated by high resolution transmission electron microscope.
Resumo:
丙型肝炎病毒(Hepatitis C virus, HCV)的全基因组序列测定,曾经由于许多方面 的条件限制而难于完成。但是,其对于研究HCV 分子病毒学、流行病学、进化和致 病性却至关重要,特别是在临床应用中,不同序列的基因型决定α-干扰素治疗的不同 效果。在本研究完成之前,HCV 基因型6 仅有6 个亚型有其全基因组序列。因此, 本研究的主要目的在于,测定HCV 变异株代表基因型6 其余的11 个亚型和新亚型的 全基因组序列,并深入分析。 本研究从样品分别来自于中国、泰国,和在美国及加拿大生活的东南亚国家移民 的HCV 感染者。因为样品有限,改良传统的PCR 方法,摸索出“桥”和“岛”DNA 全序列扩增法,从每例样品100μl 血清或从100μl 血清中获得的cDNA 中测定了13 个HCV 全基因组核苷酸序列。 以来源于Genbank 的已知基因型6 的七个全长序列为参考对所测定的13 个亚型 全序列进行共同分析显示,这些全基因组核苷酸的两两比较相似率变化范围为 71.9%--82.7%,著地, 这四对序列间的相同率高于标准定义的HCV 基因亚型之间的 范围值75%-80%。为了进一步理解和证实这些亚型间的遗传相似性,本研究还测定 了代表这4 对亚型的病毒原型株的全基因序列,结果显示了相同的核苷酸水平上的变 异范围,这为HCV 基因亚型的分类提供了新的认识,亦强调了全长序列对于分类的 重要性。 从系统发育方面的分析证实,本研究所测得的13 个分离株都属于基因型6。在系 统发育树上,每个病毒株代表一个独立的枝。并形成了高度分化的HCV 基因型6 分 枝,从而清楚显示,各亚型的独立分布。本研究至此完成了基因型6 中17 个亚型的 全序列测定,而km41 和gz52557 因缺乏其临床上和流行病学上的多个感染病例的证 实,而继续保留其亚型未命名状态。结合来源于Los Alamos HCV database 的基因型6 的已知部分序列的变异株进一步分析,发现各相近亚型变异株均来自东南亚或东南亚国家移民,这提示了这些HCV 的相同感染源。 为了探讨HCV 夫妻间传播的可能性,本研究还测定了来自于泰国的两位感染 HCV 的献血员及其感染HCV 的配偶。这4 个基因序列C-0044 和C-0046 之间核苷 酸相同率为98.1%,而C-0185 和 C-0192 之间为97.8%。文献研究感染HCV 的夫妇 间的部分亚基因序列的相同率为96.3%至100%,本研究结果与此范围相符,并第一 次用全基因组序列提示了HCV 在夫妻间传播的可能性。 本研究还测定了基因型6 的另一个变异株的全基因组序列:HK6554,香港的某患 者,与上文中的GX004 一起,均为静脉吸毒者,并共同感染了HCV 和HIV-1。分析 结果还表明了一种趋势,即是在中国南方,基因亚型6e 有从以前的地方性传播方式 转为现有的流行性传播方式。这种转变可能由于静脉吸毒感染HCV 的人群的网络传 播而加快。 综上,本研究用传统PCR、简并引物结合链特异引物的方法有效地测定了共21 个病毒株的全基因序列。该方法也可用于其它分子流行病学的研究,特别在测定珍贵 的病毒序列然而样品量又受限时。本研究所测定的全基因组序列代表HCV 中最古老、 分化最多、地方性传播、又可能动物源性的基因型6 的全套17 个亚型。这有助于进 一步理解HCV 基因亚型的分类意义、更准确评价HCV 的进化和起源,亦有助于发 现HCV 新的变异株和提高临床诊断、治疗,为将来HCV 的流行及公众健康的预测、 预防和疫苗的制备奠定了坚实的分子遗传学基础。
Resumo:
在黑白仰鼻猴(Rhinopithecus bieti)分布区北端的南仁(99o04’E, 28o34’N), 野外工作分别于2001年4月10日 - 6月30 日(代表冬末和春季),9月14日 - 12月20日(夏秋季)进行。我们分别用粪便取样法、录像带记录和直接观察法收集了猴群生境的垂直利用、过夜处选择和社会组织数据。此外,我们于1998年8月20日到12月31日在中科院昆明动物研究所老所利用全发生取样法(All-Occurence sampling)收集了一个单雄多雌单元(One-male, multi-female unit: OMU)的性行为数据。另外,我们利用昆明动物所1994 - 2003年和昆明动物园1991 - 2003年笼养黑白仰鼻猴群的出生记录来说明出生季节和出生间隔。 黑白仰鼻猴群全年在3500 - 4300 m的林带上活动,集中利用的海拔带为3900 - 4200 m,这可能与猴群的主食(松萝)主要分布于高海拔有关。冬季, 山沟中的粪便密度高于山脊,这可能是猴群在沟中过夜的缘故。猴群喜欢在树高(27.5 ± 3.2 m)较高、胸径(57.9 ± 16.9 cm)和树冠(6.3 ± 1.4 m)大的针叶树(云冷杉)上过夜。猴群冬季喜欢在阳坡中部的针叶树上过夜,这样既安全又可以接受适量的阳光照射。这是猴群在选择最安全和最暖和过夜处的一种折衷策略。 1994年猴群OMUs大小为7.8 ± 1.7(n = 17),成年性比(M/F)是1.0: 3.8。2001年OMUs大小为10.1 ± 3.7 (n = 15),成年性比是1.0: 4.9。1994-2001年,OMUs中每个成年雌性每年的平均增长率是0.04。这种OMU-band两层社会组织与Kirkpatrick(1996)的报道一致。 雌性以匍匐地面或栖木上,同时面部和视线左右摆动,或者坐着上下移动头部的动作邀配;雄性则以伴有特别的叫声、露齿动颌表情邀配。在有射精记录的观察日中,平均每5.2次爬跨有1次射精,而单次爬跨就射精的仅占4.4%。雌性邀配了18次射精爬跨的大多数(72%),但163次非射精爬跨中她们邀配的仅为45%。雄性在射精交配中叫声多于非射精交配。该种交配模式与其它疣猴亚科动物相似,而性内交配竞争可能与这种模式的进化有关。 笼养黑白仰鼻猴群的出生日期为12 - 6月份,出生高峰期为3 - 5月份。猴群的平均出生日期为4月18日(标准差为43天),中位出生日期为4月10日。猴群的出生间隔平均为624 ± 150天(n = 15,范围:332 - 787天)。幼猴可活到1岁后的出生间隔(706 ± 71, n = 12, 498 - 787天)显著长于1岁内死亡或流产后的出生间隔(428 ± 87, n = 5, 332 - 568天)。婴猴性比(M/F)显著偏离1: 1。
Resumo:
高功率激光二极管列阵广泛应用于抽运固体激光器.报道了17 kW GaAs/AlGaAs叠层激光二极管列阵的设计、制作过程和测试结果.为了提高器件的输出功率,一方面采用宽波导量子阱外延结构,降低腔面光功率密度,提高单个激光条的输出功率,通过金属有机物化学气相沉积(MOCVD)方法进行材料生长,经过光刻、金属化、镀膜等工艺制备1 cm激光条,填充密度为80%,单个激光条输出功率达100 W以上;另一方面器件采用高密度叠层封装结构,提高器件的总输出功率,实现了160个激光条叠层封装,条间距0.5 mm.经测试,器件输出功率达17kW,峰值波长为807.6 nm,谱线宽度为4.9 nm.