Properties of the proton-evoked currents and their modulation by Ca2+ and Zn2+ in the acutely dissociated hippocampus CA1 neurons

The characterization of acid-sensing ion channel (ASIC)-like currents has been reported in hippocampal neurons in primary culture. However, it is suggested that the profile of expression of ASICs changes in culture. In this study, we investigated the properties of proton-activated current and its modulation by extracellular Ca2+ and Zn2+ in neurons acutely dissociated from the rat hippocampal CA1 using conventional whole-cell patch-clamp recording. A rapidly decaying inward current and membrane depolarization was induced by exogenous application of acidic solution. The current was sensitive to the extracellular proton with a response threshold of pH 7.0-6.8 and the pH(50) Of 6.1, the reversal potential close to the Na+ equilibrium potential. It had a characteristic of acid-sensing ion channels (ASICs) as demonstrated by its sensitivity to amiloride (IC50 = 19.6 +/- 2.1 muM). Either low [Ca2+](0) or high [Zn2+](0) increased the amplitude of the current. All these characteristics are consistent with a current mediated through a mixture of homomeric ASIC1a and heteromeric ASIC1a + 2a channels and closely replicate many of the characteristics that have been previously reported for hippocampal neurons cultured for a week or more, indicating that culture artifacts do not necessarily flaw the properties of ASICs. Interestingly, we found that high [Zn2+] (>10(-4) M) slowed the decay time constant of the ASIC-like current significantly in both acutely dissociated and cultured hippocampal neurons. In addition, the facilitating effects of low [Ca2+](0) and high [Zn2+](0) on the ASIC-like current were not additive. Since tissue acidosis, extracellular Zn elevation and/or Ca2+ reduction occur concurrently under some physiological and/or pathological conditions, the present observations suggest that hippocampal ASICs may offer a novel pharmacological target for therapeutic invention. (C) 2004 Elsevier B.V. All rights reserved.

Stress evoked by opiate withdrawal facilitates hippocampal LTP in vivo

Stress impairs hippocampal long-term potentiation (LTP), but it is unknown whether the stress evoked by opiate withdrawal has the same effect. Here the authors report that opiate withdrawal for 4 days does not influence basal synaptic transmission, but re

新生期触觉刺激和母婴分离对雌性大鼠成年后的焦虑和情绪记忆的影响

采用split-litter法对仔鼠进行分组和处理,共5组NTS组(未经实验人员抓握和标记),PND2—9TS组和PND10—17TS组(分别在仔鼠出生后的2—9天、10—17天,每天短暂抓握和标记仔鼠),PND2—9MS组和PND10—17MS组(分别在仔鼠出生后的2—9天、10—17天,除了按TS组相同方式抓握并在不同部位标记外,每天把仔鼠与母鼠分离1h)。待雌鼠成年后,进行明/暗箱测试和一次性被动回避反应测试。结果发现与NTS组相比,PND2—9TS组和PND10—17TS组的雌鼠在明/暗箱测试中停留于明室的累计时间明显较长,在被动回避作业中的重测试潜伏期也明显较长,表明新生期的触觉刺激经历减少雌性大鼠成年后在新异环境中的焦虑,并改善情绪记忆。与相应TS组相比,MS处理组的所有行为指标都无显著性差异,说明短时间母婴分离对雌鼠成年后的焦虑和情绪记忆无明显影响。结果提示,新生期的触觉刺激和母婴分离经历对仔鼠神经系统的发育产生不同的长期效应。

复合垂直流构建湿地基质酶活性与污水净化效果

构建湿地是 2 0世纪 70年代兴起的处理污水的生态工程 ,由于其具有建造、运行和日常管理费用低廉 ,处理效果稳定 ,且适用面广 ,越来越受到世界各国的重视。这种污水处理技术在发展中国家有着十分广泛的应用前景。本文研究与揭示了复合垂直流构建湿地基质中的磷酸酶和脲酶活性及与污水净化效果的关系 :1不同类型湿地基质酶活性不同 ,甚至不同月份的酶活性也不相同。2不同深度基质中的酶活性是不相同的。 3基质磷酸酶的活性与复合垂直流构建湿地对污水中总磷 ( TP)、无机磷 ( IP)以及化学需氧量 ( CODCr)的

蓝藻色氨酸调节型突变体的筛选和分析

自发或经MNNG诱变获得了22株稳定的鱼腥藻1017抗6—氟色氨酸(6FT)突变体。有些突变体分泌过量的色氨酸.并能供应给色氨酸营养缺陷型大肠杆菌.使其周围形成明显的生长圈,它们中的一些还能缓解6FT对野生型生长的抑制作用。突变体6FT—1细胞内色氨酸含量与野生型相近,但分泌到培养基的色氨酸量为野生型的2～3倍。外加6FT能促进突变体的细胞分泌色氨酸和蛋白质。外源芳香类氨基酸对鱼腥藻1017的生长有抑制作用。以上结果暗示鱼腥藻1017色氨酸生物合成可能存在某些在蓝藻中尚来报道的机理,其中包括存在受色氨酸(

柄杆菌与固氮蓝藻培养——Ⅰ.多态柄杆菌的分离和鉴定

本文报道了血腥藻(Anabaena spp.)的一种异常生长现象和从鱼腥藻中分离得到的柄杆菌1017—41株的生物学特性。此菌株除具有柄杆菌特有的形态和发育周期外,还出现分叉状和长的分节状菌体,与已有记载的柄杆菌明显不同,故定为柄杆菌属(Caulobacter)的一个新种,命名为多态柄杆菌(Caulobacter polymorphus nov.sp.)。

C-values of seven marine mammal species determined by flow cytometry

C-values, which estimate genome size, have puzzled geneticists for years because they bear no relationship to organismal complexity. Though C-values have been estimated for thousands of species, considerably more data are required in order to better understanding genome evolution. This is particularly true for mammals, in which C-values are known for less than 8% of the total number of mammalian species. Among marine mammals, a C-value has been estimated only for the bottlenose dolphin (Tursiops truncatus). Thus examination of additional species of marine mammals is necessary for comparative purposes. It will enable a better understanding of marine mammal genome evolution, and it is also relevant to conservation, because larger genome size has been linked to increased likelihood of extinction in some plant and animal groups. Our study presents C-values of seven marine mammal species, including five cetacean species that are endangered to varying degrees. Similarly to the results for other groups, our results suggest that larger genome size in cetaceans is related to an increased likelihood of extinction.

基于草图界面的设计方案复用研究

当前的CAD工具缺乏对构思活动中各种设计知识的有效建模,造成设计方案难以在后续工作中灵活、快速地复用.采用基于草图界面的自然交互方式,通过对设计过程中草图信息的分析与建模实现对设计过程的描述;在复用过程中向用户提供推荐方案,支持设计意图的自然表达和方案的灵活复用,从而降低用户认知负担.实验结果表明,这种基于草图信息分析与建模的设计方案能满足敏捷设计的需要,实现了设计方案的灵活复用.

厌氧-好氧生物处理绢麻废水

本文介绍了广州绢麻纺织厂通过试验提出的用厌氧-好氧生物处理绢麻废水的工艺流程和处理效果。

偶氮类电光聚合物薄膜非线性定量研究

介绍了主客掺杂NAEC/PMMA电光极化聚合物薄膜的制备方法，测得其紫外可风吸收光谱和在1064 nm波长下的二阶非线性系数X_(33)~((2))，为23.6 pm/V；采用两态模型，对X_(33)~((2))进行了理论估算，作出了其色散曲线，得到理论值与实验值符合较好的结论。估算极化电场强度为2.638 MV/cm。

多量子阱光学波导中的模式截止

导出由两种材料构成的多量子阱光波导的模式截止方程,并讨论了Si衬底上生长的应变Ge_xSi_(1-x)/Si多量子阱光波导的模式截止特性.