Study on bioactivity of extracts from marine sponges in Chinese Sea

The bioactivity screening of fractions from two inter-tidal sponges collected from the north of China Yellow Sea and one sponge collected from the South Chinese Sea was reported in this study. In sponge Hymeniacidon perleve there were 9 fractions out of 15 from CHCl3 extract with anti Staphylococcus aureus activity, 9 fractions out of 19 from BuOH extract with anti Escherichia coli activity, and three fractions from CHCl3 extract which had moderate to strong activity in inhibiting Bacillus subtilis, Candida albicans, and Aspergilus niger. The fractions of Reniochalina sp. showed bioactivity against bacteria and fungi. The fractions of Acanthella acuta Schmidt showed bioactivity against S. aureus and fungi. One compound from H. perleve obtained by the bioactively directing isolation was tested for bioactivity against the human hepatoma cell line Qgy7701 (IC50 10.1 mug/ml), Burkitt's lymphoma cell line Raji (IC50 9.76 mug/ml) and chronic myelogenous leukemia K562 (IC50 1.90 mug/ml). (C) 2003 Elsevier B.V. All rights reserved.

Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis

Marine sponges (Porifera) possess an extraordinary diversity of bioactive metabolites for new drug discovery and development. In vitro cultivation of sponge cells in a bioreactor system is very attractive for the sustainable production of sponge-derived bioactive metabolites; however, it is still a challenging task. The recent establishment of sponge primmorphs, multicellular aggregates from dissociated mixed-cell population (MCP), has been widely acknowledged to hold great promise for cultivation in vitro. Here we present a new method to establish an in vitro sponge primmorph culture from archaeocyte-dominant cell population (ADCP) enriched by a Ficoll gradient, rather than a mixed-cell population (MCP). Our rationale is based upon the totipotency (the ability of a cell to differentiate into other cell types) of archaeocyte cells and the different biological functions of various sponge cell types. A sponge, Hymeniacidon perleve collected from the China Yellow Sea was used as a model system for this investigation. Distinct dynamics of primmorph formation were observed while significant increases in DNA synthesis, cell proliferation (up to threefold), and cell growth (up to fourfold) were achieved. Furthermore, a time-dependent spiculogenesis was clearly demonstrated in our longterm culture, indicating high metabolic activity of primmorphs from the ADCP. This new method represents an important step forward to advance sponge cell culture in vitro that may lead to commercial exploitation of sponge-derived drugs. (C) 2003 Wiley Periodicals, Inc.

Direct spectroscopic evidence for the specific property of aqueous core phase in black soap film

The microenvironment of the aqueous core phase in the black soap film of cationic surfactant cetyltrimethylammonium bromide with the anionic dye Brilliant Yellow as spectral probe has been studied by UV-vis spectroscopy. Under neutral and basic conditions, the dye aggregates in the films exist as both the acid and base forms in contrast to a preference of the base form in the bulk solutions. The specific property of black soap film, that the intrinsic pH value of the aqueous core phase insensitively responds to pH changes of the bulk solution, is directly observed through UV-vis spectra.

黄土高原水土保持的水文环境效应研究进展

黄土区大面积、高强度的植被营造工程和各项水利工程虽充分利用了有限的水资源 ,减少了入黄泥沙 ,但同时也人为的切断或改变了水分循环的途径 ,对水环境产生不同方向和程度的影响。尽管国内外学者对水土保持的水环境效应已进行了不少研究 ,但仍存在一些问题 ,主要表现在单项措施 ,特别是林草措施下的水环境效应研究比较深入 ;在水环境研究内容上 ,多注重对地表径流减水减沙的分析 ,而对水量平衡、水文情势等研究较少 ;在研究尺度上 ,多集中于对径流小区的分析 ,单点研究深入而面研究不够

欧报春(Primula vulgaris)不同花色与色素关系及花色遗传初步分析

为了掌握欧报春各花色遗传规律服务于良种生产,通过对欧报春各色花进行色素吸收光谱和薄层层析分析,进行不同花色杂交研究,分析了欧报春各色花所含色素类型及各花色遗传规律。结果显示欧报春群体含多种花色素,单株也可含有多种花色素,形成多变的粉色、红色及蓝色花。黄色深浅主要由类胡萝卜素含量决定。白色对粉色及黄色为隐性遗传,黄色、粉色为显性遗传并有数量遗传特征,黄色与粉色独立遗传。蓝色为多基因控制的隐性遗传,并具有数量遗传特征。

塿土坡面降雨水沙运移实验研究

黄土高原是我国植被破坏与水土流失最为严重的地区。本文基于室内坡地降雨实验,分析了塿土坡面降雨入渗补给系数的变化,对比了不同雨强影响下径流泥沙含量和累积泥沙含量的时间变化趋势,并将侵蚀与入渗结合,分析了同时段产沙量和入渗率的变化关系,研究表明:入渗补给系数随雨强增大而逐渐减小,两者可描述为线性关系,雨强越小,入渗补给系数越高;降雨初始产沙值最大,在产沙10 min内变缓。雨强大的,初始产沙量和稳定沙量都稍大,但总体差异不大,产沙量分布曲线形状与入渗率曲线极为相似;不同雨强下累积泥沙量与降雨历时之间存在明显的幂函数关系,且幂函数的指数部分基本随降雨雨强增加而逐渐增加;不同雨强下入渗率和累积泥沙量的变化趋势相反,当累积泥沙量增加,相应的土壤入渗率减小,两者变化趋势随雨强增大而趋于明显。

水蚀风蚀交错带小流域生态环境综合治理模式研究

:水蚀风蚀交错带是黄土高原侵蚀最严重的地区和黄河下游河床粗泥沙的主要来源区 ,其生态环境脆弱、治理难度大 ,加之晋陕蒙能源基地的大规模开发 ,也带来了新的环境问题。鉴于治黄和煤田开发及生态环境整治的紧迫需要 ,以水蚀风蚀交错带六道沟小流域为试区 ,分析研究了该区水蚀风蚀时空分布规律及脆弱生态环境特征 ,提出以防治水蚀和风蚀为中心 ,以提高生态经济效益和持续发展为目标 ,以基本农田优化结构和高效利用及植被建设为重点 ,建立具有防蚀固沙兼高效生态经济功能的大农业复合生态系统综合治理模式 ,取得了良好的社会与经济效益

黄土高原水蚀风蚀交错区治理的重要性与紧迫性

在全国范围划分水蚀、风蚀和冻融侵蚀三大侵蚀类型区基础上 ,提出进一步划分水蚀风蚀交错区的必要性及重要意义。黄土高原的强烈侵蚀中心出现在水蚀风蚀交错区 ,该区为黄河粗泥沙的主要来源区 ,生态环境脆弱 ,自然灾害频繁。该区又为世界级大型煤田蕴藏地 ,将建成我国 2 1世纪的能源重化工基地。强化水蚀风蚀交错区综合治理 ,对治黄及西部地区开发具有重大意义。

抢救“土壤水库”实为黄土高原生态环境综合治理与可持续发展的关键——四论黄土高原国土整治28字方略

现在全国上下深切关注着黄河的重大问题及其对策。其实问题的根源都出自人们对黄河流域尤其黄土高原自然资源的掠夺式开发 ,形成了“3个恶性循环”:广种薄收 ,薄收更广种 ,虽是罪魁祸首 ,但常反被忽视 ,致使生态环境脆弱 ,人民长期贫困 ;只有下游河床越淤越高 ,防洪大堤越筑越高 ,越高越险和断流历时越来越长 ,上溯速度越来越快 2个恶性循环的危害才使人惊恐。不难看出 ,这是恰与形成黄土高原自然规律完全相悖的人为地质过程的结果。可惜它还未引起人们深入充分的认识 ,难怪一向短缺一个为各家合力共识的治本对策。当今国家将经济发展的重点向中、西部转移 ,并要求重建一个山川秀美的大西北 ,黄土高原的持续开发与治理对策就显得更为举足轻重。由此其各项对策都将面临着转变观念、调整思路、实事求是地进行科学分析和抉择。笔者最近从陆地生态的发生发展及其整个地质历史演变过程的研究中发现 :“土壤水库”的发生发展及其演变是陆地生态发生发展的关键和“动力”,只要维护土壤水库的正常发展就能更好地保卫生态环境。黄土高原地区由于得天独厚的降尘堆积环境条件和持续的成壤过程 ,可使降水具有直接渗入“地下水库”的特殊功能。只要维护住高入渗土壤水库的存在就...

黄河中游地区泥沙变化研究进展

自 2 0世纪 80年代起 ,有关黄河中游地区泥沙变化的研究已取得了丰富的成果。从黄河中游地区泥沙变化的时空差异、泥沙变化研究中存在的问题、未来泥沙变化的预测等 3个方面分析总结了目前的研究现状 ,并在此基础上提出了自己的看法。

黄河流域泥沙矿物成分与分布规律

黄河及其干支流经常产生高含沙水流,泥沙中矿物的成分及含量是影响高含沙水流性质的主导因素,粘土矿物颗粒含量对高含沙水流流变性质的影响,远大于颗粒粒径大小对流变性质的影响,粘土颗粒和阳离子之间的相互作用对高含沙水流的流变性质也有非常大的影响。通过对黄河及其支流地区12个地点进行采样分析,得出黄河及其支流地区泥沙基本来源于同一地区,次生矿物是在长期的、相同的环境条件作用下形成的。根据泥沙样本的粒径分布曲线和矿物成分及含量,建立了黄河及其支流泥沙粘土、非粘土矿物含量与中值粒径之间的关系,能够更方便、快捷、廉价地估算黄河及其支流粘土矿物的含量。

流域尺度不同水保措施减水效益分割

在实测资料的基础 ,借助流域网格划分的模型计算方法 ,首次在流域尺度内将水土保持工程措施和生物农业措施在减少地表径流中的作用分割开来 ,从而为定量评价黄土高原以林草植被建设为主的生态环境治理对黄河流域水资源的影响提供数据基础。计算结果显示 ,在“六五”基础上王东沟小流域以梯田建设、土地平整、沟坡道路防蚀技术为主的水土保持工程措施平均减少地表径流 18.1% ,而在“七五”基础上以人工林草植被建设为主的生物措施和调整土地利用结构、扩大经济林果种植面积的农业措施共减少地表径流 10 .9%。这一结论说明为最大限度地减少黄土高原生态环境建设对黄河水资源的影响 ,其治理应以生态、农业措施为主。

基于RS的黄河中游多沙粗沙区土地利用变化分析

据1975年的Landsat MSS、1986年和1997年的Landsat TM影像资料,运用遥感影像计算机自动分类方法获取土地利用信息,用GIS空间分析方法以及数理统计方法全面分析了黄河中游多沙粗沙区1975~1986年和1986~1997年两个时期内各土地利用类型的变化幅度、变化速度、数量变化的区域差异、变化方向以及变化方向的区域差异等。结果表明:后期土地利用类型间的相互转化有所增强;耕地、草地、林地和未利用地是本区土地利用变化的主导类型,耕地、草地与其它土地利用类型间的相互转化分布校广;后期耕地被居民地占用的面积和毁林开荒的面积比前期有所增加。

Relationship between species diversity and ecotope diversity

The relationship between species diversity and ecotope diversity has long been debated. But these debates seem meaningless because most of them were based on different definitions. In this paper, diversity has two components: richness based on the total number and evenness based on the relative abundance. Species diversity is distinguished into individual-counting diversity and biomass-based diversity. Ecotope diversity is divided into individual ecotope-counting diversity and ecotope-area based diversity. Under this definition, we make a comprehensive investigation into Dongzhi tableland of Loess Plateau by cooperating with local technicians. We find that individual-counting diversity is significantly correlated with biomass-based diversity in grassland ecosystems; individual ecotope-counting diversity and ecotope-area based diversity have a significant correlation. Therefore, it is unnecessary to divide species diversity into individual-counting diversity and biomass-based diversity in grassland ecosystems and to distinguish ecotope diversity into individual ecotope-counting and ecotope-area based diversity for the issues that have no special requirement for accuracy. But the analyses of the investigation data demonstrate that species diversity has no significant correlation with ecotope diversity.

Robust Tris-Cyclometalated Iridium(III) Phosphors with Ligands for Effective Charge Carrier Injection/Transport: Synthesis, Redox, Photophysical, and Electrophosphorescent Behavior

With the target to design and develop new functionalized green triplet light emitters that possess distinctive electronic properties for robust and highly efficient phosphorescent organic light-emitting diodes (PHOLEDs), a series of bluish-green to yellow-green phosphorescent tris-cyclometalated homoleptic iridium(III) complexes [Ir(ppy-X)(3)] (X=SiPh3, GePh3, NPh2, POPh2, OPh, SPh, SO2Ph, Hppy=2-phenylpyridine) have been synthesized and fully characterized by spectroscopic, redox, and photophysical methods