红发夫酵母发酵虾青素的研究

红发夫酵母分离于北美西部高山地区和日本一些岛屿上落叶树的渗出液中，因其所产主要色素为在水产养殖、食品和医药工业有广阔应用前景的虾青素而成为研究的热点。本论文对红发夫酵母Phaffia rhodozyma 的生长特性、培养参数与培养基组分对生长和虾青素积累的影响及其优化、虾青素合成的调节控制、虾青素的提取测定及红发夫酵母耐高温菌种的诱变进行了系统的研究。 虾青素是红发夫酵母的胞内色素，要对其进行分析首先要对红发夫酵母进行破壁处理，实验发现二甲亚砜是最有效的破壁溶剂，用氯仿和丙酮可以有效地把类胡萝卜素从二甲亚砜破壁后的红发夫酵母细胞中提取出来。 在固定摇床转速为200 rpm，温度为20 ℃的条件下，当种龄为36 h，以10％的接种量接入装液量为30 mL的250 mL三角瓶，初始pH为5.5时最有利于红发夫酵母的生长及类胡萝卜素的合成。 本实验中红发夫酵母最佳利用碳、氮源分别为蔗糖和蛋白胨，但蛋白胨价格昂贵，不适宜作单一氮源，因此使用硫酸铵和酵母膏作为复合氮源。 本论文采用了BP神经网络结合遗传算法的方法来优化红发夫酵母的发酵培养基，得到红发夫酵母发酵培养基的最佳配比为：蔗糖45.10 g/L、硫酸铵3.00 g/L、硫酸镁0.80 g/L、磷酸二氢钾1.40 g/L、酵母膏3.00 g/L、氯化钙0.50 g/L，使用优化后的培养基发酵类胡萝卜素产量达到8.20 mg/L，干重达到9.47 g/L，类胡萝卜素的产量比起始培养基提高了95.90%，干重提高了89.40%。 从代谢途径出发对红发夫酵母合成虾青素调控调控，选择谷氨酸、乙醇、VB1作为添加剂，通过正交试验设计得出三者添加水平分别为0.2 g/L，0.1% (V/V)，10 mg/L时，类胡萝卜素产量提高了25.73%，达到了10.31mg/L。 通过上述优化培养，本论文中红发夫酵母的虾青素产量从1.33 mg/L提高到9.12 mg/L，产量提高了6.86倍；总类胡萝卜素产量从4.23 mg/L提高到10.31 mg/L，产量提高了2.44倍；细胞干重从5.00 g/L提高到11.35 g/L，提高了2.27倍，总体提高效果显著。 红发夫酵母属于中低温菌，本论文采用紫外复合诱变的方式，通过高温筛选，得到一株能在35 ℃下能生长的突变株，但所产类胡萝卜素中虾青素所占比例很小，可能是诱变改变了红发夫酵母的代谢途径，阻断了虾青素的合成。 Phaffia rhodozyma is a heterobasidiomyceteous yeast that was originally isolated from the slime fluxes of brich tree wounds in mountain regions of northern Japan and southern Alaska. Phaffia rhodozyma produces astaxanthin as its principal carotenoid pigment, which has potential applications in acquaculture, food and pharmaceutical industry. This paper researched ways to break cell, analysis of astaxanthin, characteristics of growth, culture parameters and the effects of components of medium on growth and astaxanthin formation , optimization of culture medium, control of astaxanthin synthesis and mutagenesis of Phaffia rhodozyma. It is necessary to disrupt the yeast cell for extracting astaxanthin considering the yeast accumulating carotenoids in cell. Dimethyisulphoxide was the most effective solvent for breaking the yeast cell; acetone and chloroform were effective to extract carotenoids out of the disrupted cell. The optimum pH for growth and carotenoids synthesis is 5.5, the optimum medium volume is 30 mL (in 250 mL flask), the optimum culture time of inoculum is 36 h, the optimum inoculum concentration is 10%. The research on culture medium showed: sucrose is the best one of 6 carbon sources for growth and astaxanthin synthesis. Peptone is the best nitrogen source for growth and astaxanthin synthesis. Uniform Design was used for trial design of the formula medium components, then back-propagation neural network was established to modeling the relationships between the carotenoid yield and the concentration of medium components. Genetic algorithm (GA) was used for global optimization of the model. The optimum combination of the medium was obtained: sucrose 45.10 g/L, ammonium sulfate 3.00 g/L, magnesium sulfate 0.80 g/L, potassium dihydrogen phosphate 1.40 g/L, yeast extract 3.00 g/L, calcium chloride 0.50 g/L. The yield of carotenoid reached 8.20 mg/L, which was 95.90% higher than that of the original medium. Glu, VB1 and ethanol were selected as fermentation addictives, after Orthogonal Test, the carotenoid contents increased by 25.73% when adding 0.16 g/L Glu, VB1 10 mg/L and ethanol 0.1% (V/V). After the above optimization, the astaxanthin content increased 6.86 folds, which is 9.12 mg/L. The carotenoids content increased 2.44 folds, which is 10.31 mg/L. The biomass increased 2.27 folds, which is 11.35 g/L. Phaffia rhodozyma grows in the mild temperature range of 0 to 27 ℃, in this work, a thermotolerant mutant was selected through UV-irradiation. It can grows at 35 ℃, and showed increased carotenoid content. The optimal growth temperature for this mutant is 30 ℃. But the mutant can only produce carotenoids with little astaxanthin accumulation.

Red clay sediment in the central Chinese Loess Plateau and its implication for the uplift of the Tibetan Plateau

IEECAS SKLLQG

长白山地区曲尾藓属植物生态分化的排序研究

The relationships of eight moss species of Dicranum in 31 sites in main ecological systems in the Changbai Mountain with environmental factors were studied by canonical correspondence analysis (CCA). The results showed that altitude, soil sand percentage, water percentage, acidity and canopy density were important environmental factors influencing the distribution of the species of Dicranum . The relationships between Dicranum elongatum Schleich. ex Schwaegr ., D.groenlandicum Brid. and altitude,between D.japonicum Mitt., D.scoparium Hedw. and canopy density,between D.polysetum Sw., D. undulatum Schrad. ex Brid. and soil acidity and water percentage,were positively correlative. The niche overlaps among the eight species of Dicranum were calculated. The minimal spanning tree of the eight species on the two-dimensional scatter plot were also drawn based on their niche overlaps, which clearly revealed the ecological similarities of eight species.

长白山红松针阔混交林CO_2通量的日变化与季节变化

Diurnal and seasonal variation of CO_2 flux above the Korean Pine and broad_leaved mixed forest in Changbai Mountain were expounded according to the measurements by eddy covariance technique. The results showed that the diurnal variation during growing season was closely correlated with photosynthetically active radiation (PAR). The forest assimilated the CO_2 in daytime and released in night. The maximum uptake occurred about 9 o'clock of local time in clear day. Assimilation was synchronous to PAR in cloudy day. The night respiration increased with increasing of shallow soil temperature. The CO_2 flux also had obviously seasonal variation that was mainly controlled by temperature. Relationship between monthly net exchange of CO_2 and monthly mean air temperature fit cubic equation. Remarkable uptake occurred in blooming growing season,May to August,and weak respiration occurred in dormant season,October to March,and relatively big release happed in October. Assimilation and respiration were nearly balanced during the transition of growing and dormant seasons. The annual carbon uptake of the ecosystem was-184 gC·m -2 .

长白山阔叶红松林夏季温度特征研究

Air and soil temperatures were observed in and out of broadleaved P.koriaensis forest (average height 25m) at Changbai Mountain in July,2000.The results showed that there was a very obvious difference between temperatures in and out of forest and both of them showed diurnal variation.Air temperature in the forest at 2m height was always lower in daytime and higher at nighttime than that in the open air.Air temperature at height of 22m in the forest was higher than that at 2m.The difference of air and soil temperature at the two sites in clear day was larger than that in cloudy day,and the latter was larger than that in overcastday.Reasons were also explained.

浑太流域洪涝灾害及其治理方略

Hunhe, Taizihe rivers originating from the east mountain areas of Liaoning Province are the biggest tributaries of the left bank of Liaohe River. From the initial stage of New China to the early 1990s, the frequency of flood damage had decreased, but recently the disasters have upgraded and changed from trunk stream to tributary basins. In July 1995, Dongzhouhe, a tributary of Hunhe River, suffered from a heaviest flood since establishing the document of floods in Hunhe basin. Meanwhile, a peak discharge, almost beyond double defending ability, occurred at Dadongshanpu Station of Beishahe River, a tributary of Taizihe River. Besides the continued heavy rainstorm, no controllable water conservancy projects and ecological damages caused by quickly decreasing forest cover and water and soil loss are the two main causes of flood damage. In addition, the low flood protection standard is also one of causes. According to the above analyses, some strategies of synthetic controlling for flood damages were put forward: 1) Establishing the system of ecological engineering to defend floods and reduce disasters; 2)Setting up storage and detention areas; 3) Establishing flood controlling information system; 4) Improving the accuracy of media short period and short period rainstorm forecast and flood forecast; 5) Insurance of flood protection.

云雾山天然草地植物生长规律

试验以云雾山优势种本氏针茅(Stipa bungeana)、大针茅(Stipa grandis)与伴生种百里香(Thymus mongolicus)、铁杆蒿(Artemisia sacrorum)、厚穗冰草(Agropyron dasystachys)、二裂萎陵菜(Potentilla bifurca)和阿尔泰狗娃花(Heteropappus altaicus)为研究对象,对每一植物选择典型植株进行标记,重点从植物株高和地上单株生物量两个方面对其生长动态,进行一个生长周期(返青期-枯黄期)的观察测量,结果表明:(1)植物生长在株高和地上单株生物量达到最大值之前,均基本符合Logistic生长模型;(2)在植物整个生长过程中,地上单株生物量月变化与株高存在动态分形关系:lnB=D×lnH+lnC。(3)植物生长除受其生物学特性支配外还受外界环境因子的限制,在干旱半干旱地区,降雨量显得尤为重要,在本次试验中,植物株高增长和地上单株生物量月变化均与降雨量累积存在着极显著的对数相关性:y=a×lnx+b。

黄土丘陵区次降雨下的山坡道路侵蚀特征分析

黄土丘陵区是水土流失治理的重点区,由于大规模以植被恢复重建的生态建设和以石油资源开发引起的山区道路建设对本地区侵蚀环境和侵蚀动力机制产生了巨大影响,坡面水土流失与道路水土流失对比发生了新变化,在坡面侵蚀得到初步治理的新环境下,水土流失源于道路侵蚀则上升为主要地位。本文分析了2005年7月2日次降雨引起的支道山路(植物路)、干道山路、石油运输道路和农林草地小区、小支沟的径流、泥沙系统监测结果,提出在本次降雨条件下,三类道路的产沙强度分别为:支道山路500 t.km-2、干道山路3163 t.km-2和运油道路13500 t.km-2,而农林草地下垫面的产沙强度则仅为:5.68~184.38 t.km-2,道路产沙强度远大于农林草地的产沙强度,道路产沙量占流域总产沙量的42%。而灌草地侵蚀产沙仅占流域的26.7%。进而提出黄土高原丘陵区水土流失治理应该重点放在道路防蚀的观点,成果有助于社会主义新农村建设。

略论延安市降水资源的潜力与高效利用模式

根据在甘肃子午岭 ,安塞墩滩和延安燕儿沟的试验观测资料 ,研究了梢林区植被对降水的拦蓄和消耗情况 ;分析了农区基本农田上和退耕还林 (草 )坡地上作物和植物对降水的拦蓄和土壤水分季节动态 ;计算了本区主要农作物和几种有代表性的乔、灌、草植物的全生育期内需水量和降水资源补给的盈亏状况 ;并提出了几种利用降水资源和河川径流的模式

封育对云雾山本氏针茅草地群落的影响

以云雾山封育与未封区为对象,采用样线法进行调查,重点分析二者群落特征和地上生物量的变化,以期为退化草地植被恢复提供依据。结果表明:封育后本氏针茅(Stipa bungeana)群落发生较大变化,物种数显著增加、演替差异明显,由未封区的本氏针茅+大针茅(S. gigantea)群落演替为封育后的大针茅+本氏针茅群落;未封区本氏针茅种群的优势地位明显加强,重要值明显大于封育区;封育与未封区群落的相似性系数为0.419;物种丰富度指数和多样性指数(修正的Simpson指数、Shannon-winner指数、Audair和Groff指数)均表现为封育区>未封区,而均匀度指数则相反;封育区地上生物量明显增加,其中禾本科、蔷薇科和杂类草占总生物量的比例均表现为封育>未封区,而菊科则相反。

云雾山本氏针茅群落根系分布特征

为揭示本氏针茅(Stipa bungeana Trin.)群落的生理生态适应机制,采用根系取样器(Φ=9 cm)对宁夏云雾山本氏针茅群落根系分布特征和土壤含水量进行研究。结果表明:本氏针茅群落地下生物量、根长密度、根表面积、比根长均随土壤深度增加而减少,均表现出向表层(0~20 cm)集聚的趋势,且集中分布于0~40 cm土层,最大值均分布在0~20 cm土层,而底层(80~100 cm)最小;所有主要根系分布参数在0~20 cm和20~40 cm土层之间差异显著,以下各层差异不显著;土壤含水量与根生物量和比根长相关性达显著水平(P<0.05),与根表面积、根长密度均呈成正相关;根表面积、根生物量、根长密度和比根长间相关性均达极显著水平(P<0.01)。

黄土高原水土流失区退耕还林(草)的基本思路

朱总理针对 1 998年我国水土流失加剧而致使洪灾造成 2 2 5 1亿元人民币巨大损失的现实 ,于 1 999年 8月提出了“退耕还林 (草 )、封山绿化、个体承包、以粮代赈”的措施 ,为根治黄土高原的水土流失和良化我国的生态环境指明了方向。目前我国黄土高原地区坡耕地水土流失、肥力低下、水源缺乏、灾害频繁。根据黄土高原的生态环境脆弱的具体情况 ,应采取的对策是 :提高思想认识 ;解决好退耕后脱贫 ;推进东西部合作政策 ;保证人均一定的农田 ;实现农林牧业最佳结合 ;提出合理的措施 ;健全保障机制 ;正确处理各种关系

大巴山北坡垂直带土壤特性及系统分类

通过野外考察与室内分析相结合 ,对大巴山北坡不同海拔高度的土壤特性进行研究 ,确定了各剖面的诊断层和诊断特性及其在系统分类中的位置。结果表明大巴山北坡各垂直带土壤在系统分类中可划归 2个土纲的 6个土类 ,系统分类中土壤的分类位置与发生分类位置并不具有简单的一一对应的关系。