81 resultados para Lotus-japonicus
Resumo:
Electrowetting on dielectrics has been widely used to manipulate and control microliter or nanoliter liquids in micro-total-analysis systems and laboratory on a chip. We carried out experiments on electrowetting on a lotus leaf, which is quite different from the equipotential plate used in conventional electrowetting. This has not been reported in the past. The lotus leaf is superhydrophobic and a weak conductor, so the droplet can be easily actuated on it through electrical potential gradient. The capillary motion of the droplet was recorded by a high-speed camera. The droplet moved toward the counterelectrode to fulfill the actuation. The actuation speed could be of the order of 10 mm/s. The actuation time is of the order of 10 ms.
Resumo:
Electrowetting on dielectrics has been widely used to manipulate and control microliter or nanoliter liquids in micro-total-analysis systems and laboratory on a chip. We carried out experiments on electrowetting on a lotus leaf which is quite different from the equipotential plate used in conventional electrowetting. This has not been reported in the past. The lotus leaf is superhydrophobic and a weak conductor so the droplet can be easily actuated on it through electrical potential gradient. The capillary motion of the droplet was recorded by a high-speed camera. The droplet moved toward the counterelectrode to fulfill the actuation. The actuation speed could be of the order of 10 mm/s. The actuation time is of the order of 10 ms.
Resumo:
麦冬的花粉发育自小孢子母细胞开始,经连续型减数分裂,产生游离的单细胞小孢子;小孢子进一步发育,经两次有丝分裂,最终发育为包含有二个精细胞和一个营养核的成熟花粉。在此发育过程中,内质网、线粒体、高尔基体和脂体含量丰富。 麦冬的精子细胞为伸长的细胞,具有椭圆形的头部和长长的尾部细胞质突起,细胞器较少,是线粒体,但缺乏质体,没有明显的二型性;其精细胞伸长的尾部细胞质突起是在生殖细胞有丝分裂时产生,而不是以后由精子细胞本身发育;两个精子细胞之间除了头部的联结外,还有尾部的联接,但没有任何结构上的联结;一个精子细胞的头部常与营养核十分靠近;另外,在精子与精子之间或精子与营养核之间的贴近点上,常可见到多层膜的结构,根据精细胞之间的联结和精细胞与营养核之间的靠近,推测麦冬的成熟花粉中存在雄性生殖单位。 麦冬的花粉壁发育在四分体后期开始,首先产生纤维状的原外壁,由原外壁上依次发育出原基柱层、原覆盖层和原基足层,当四分体分离时,花粉外壁外层已发育完全;在单细胞小孢子时期,外壁内层发育,为极薄的一层,同时内壁-1开始发育;到了二细胞花粉时期,内壁-1开始发育;到了二细胞花粉时期,内壁-1发育完全,并在萌发孔处发育特殊的蜂窝状结构;紧接内壁-1层,发育出内壁-2层。在其壁发育过程中,花粉中大量沿周围平行于花粉壁排列的内质网可能与花粉壁的形成有关。 麦冬的绒毡层发育属分泌型。在小孢子母细胞时期,绒毡层细胞达到了发育的顶峰,此时,细胞器非常丰富,大量的线粒体、高尔基体和质体,尤为内质网含量最为丰富;原乌氏体出现较早,小孢子母细胞时期绒毡层细胞中就已出现;四分体时期,大量乌氏体被排入内切向面的质膜和纤维素壁之间;到了小孢子早期,绒毡层细胞失去细胞壁,原乌氏体分布在质膜的凹陷处,孢粉素物质开始在其上沉积,发育为乌氏体,乌氏体有单个和复合两种类型;到花粉成熟时,绒毡层细胞彻底解体。
Resumo:
本文应用聚合酶链式反应技术(PCR)在体外从玉米基因组中扩增富于含硫氨基酸的10KD玉米醇溶蛋白基因、克隆及序列分析结果,并用该基因转化豆科牧草百脉根以期获得有良好营养平衡的转基因牧草,从玉米无菌苗提取大片段的DNA.根据目的基因两端的DNA顺序合成一对引物,利用PCR技术经30个活环DNA扩增,得到了一特异的0.57Kb片段,克隆后对该片段进行限制性内切酶物理图谱分析并测定了其全部编码区序列.结果表明,克隆到的完整的10 Kd玉米醇溶蛋白基因编码区与国外报道的相比,其核苷酸顺序及推测的氧基酸序列同源率分别为96%和90%.将基因分别置于质粒pKYLX5及pKYLX71表达戴体,构建了分别在rbcS及CaMV 35S启动子调控下的10 KD zein基因的嵌合质粒.列用农杆菌介导的叶盘法及茎切段法,选用百脉根( Lotus corniculatus)品种一名分别为“里澳”及“马库”,以5-10天的无菌苗子叶和茎切段为外植体,进行基因转化,在含50mg/L km的MS培养基上,2-3周获得抗性的芽,将芽转到无激素的MS培养基上诱导生根,获得抗性植株,捡测工作正在进行中。
Resumo:
Climatic oscillations during the Pleistocene ice ages produced great changes in species' geographical distribution and abundance, which could be expected to have genetic consequences. Living in the temperate upwelling zones of the northwestern Pacific, Ja
Resumo:
The Quaternary cold periods in the Northwestern Pacific are thought to have heavily influenced the amount and distribution of intraspecific genetic variation in marine fishes. To estimate the demographic history and genetic structure of Lateolabrax macula