A Reliable Method for Rhythm Analysis during Cardiopulmonary Resuscitation

nterruptions in cardiopulmonary resuscitation (CPR) compromise defibrillation success. However, CPR must be interrupted to analyze the rhythm because although current methods for rhythm analysis during CPR have high sensitivity for shockable rhythms, the specificity for nonshockable rhythms is still too low. This paper introduces a new approach to rhythm analysis during CPR that combines two strategies: a state-of-the-art CPR artifact suppression filter and a shock advice algorithm (SAA) designed to optimally classify the filtered signal. Emphasis is on designing an algorithm with high specificity. The SAA includes a detector for low electrical activity rhythms to increase the specificity, and a shock/no-shock decision algorithm based on a support vector machine classifier using slope and frequency features. For this study, 1185 shockable and 6482 nonshockable 9-s segments corrupted by CPR artifacts were obtained from 247 patients suffering out-of-hospital cardiac arrest. The segments were split into a training and a test set. For the test set, the sensitivity and specificity for rhythm analysis during CPR were 91.0% and 96.6%, respectively. This new approach shows an important increase in specificity without compromising the sensitivity when compared to previous studies.

Combined Fluorescent-Chromogenic In Situ Hybridization for Identification and Laser Microdissection of Interphase Chromosomes

Chromosome territories constitute the most conspicuous feature of nuclear architecture, and they exhibit non-random distribution patterns in the interphase nucleus. We observed that in cell nuclei from humans with Down Syndrome two chromosomes 21 frequently localize proximal to one another and distant from the third chromosome. To systematically investigate whether the proximally positioned chromosomes were always the same in all cells, we developed an approach consisting of sequential FISH and CISH combined with laser-microdissection of chromosomes from the interphase nucleus and followed by subsequent chromosome identification by microsatellite allele genotyping. This approach identified proximally positioned chromosomes from cultured cells, and the analysis showed that the identity of the chromosomes proximally positioned varies. However, the data suggest that there may be a tendency of the same chromosomes to be positioned close to each other in the interphase nucleus of trisomic cells. The protocol described here represents a powerful new method for genome analysis

Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.

Advances towards behaviour-based indoor robotic exploration

215 p.

Evaluating the Role of Energy Efficiency Labels: the Case of Dish Washers

27 p.

Surf oholeko gilen elementu finitu bidezko analisia.

[EU]Lan honetan, surf oholetan erabiltzen diren gila mota desberdinen analisia egin da. Horretarako, merkatuan garrantzi handiena duten bi enpresek euren katalogoetan eskaintzen dituzten modelo batzuk aztertu dira. Ingeniaritza simulazioak ahalbidetzen dituen ansys softwarea erabiliz egin da aukeratutako modeloen diseinu geometrikoa. Hauen analisiari ekin baino lehenago, gila hauetako bat aztertu da, hau osatzen duen materiala ezagutu eta ezaugarriak zehaztu asmoz. Aztertutakoa modelo guztiek erabiltzen duten ohiko materiala izanik, lortutako emaitzak baliogarriak dira gila guztiak aztertzeko. Materialaren propietateak ezagututa eta aipatutako softwareaz baliatuz, analisi estatikoa egin da. Horretarako, surfean aurkitzen diren ohiko egoera batzuk irudikatu dira, hauetan agertzen diren esfortzuak kalkulatuz. Gila bakoitzaren portaera, jasandako tentsio eta deformazioak aztertuz ondorioztatu da. Horrela, kasu bakoitzean komenigarrienak diren ezaugarriak oinarri hartuz, modelo desberdinen arteko konparaketa egin da.