Combined Fluorescent-Chromogenic In Situ Hybridization for Identification and Laser Microdissection of Interphase Chromosomes


Autoria(s): Paz Gandiaga, Nerea; Zabala, Amaia; Royo, Félix; García-Orad Carles, África; Zugaza Gurruchaga, José Luis; Parada, Luis A.
Data(s)

07/06/2013

07/06/2013

2013

Resumo

Chromosome territories constitute the most conspicuous feature of nuclear architecture, and they exhibit non-random distribution patterns in the interphase nucleus. We observed that in cell nuclei from humans with Down Syndrome two chromosomes 21 frequently localize proximal to one another and distant from the third chromosome. To systematically investigate whether the proximally positioned chromosomes were always the same in all cells, we developed an approach consisting of sequential FISH and CISH combined with laser-microdissection of chromosomes from the interphase nucleus and followed by subsequent chromosome identification by microsatellite allele genotyping. This approach identified proximally positioned chromosomes from cultured cells, and the analysis showed that the identity of the chromosomes proximally positioned varies. However, the data suggest that there may be a tendency of the same chromosomes to be positioned close to each other in the interphase nucleus of trisomic cells. The protocol described here represents a powerful new method for genome analysis

Identificador

PLoS ONE 8(4) : (2013) // e60238

1932-6203

http://hdl.handle.net/10810/10210

10.1371/journal.pone.0060238

Idioma(s)

eng

Publicador

Public Library of Science

Relação

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0060238

Direitos

2013 Paz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

info:eu-repo/semantics/openAccess

Palavras-Chave #genome organization #cells #expression #FISH
Tipo

info:eu-repo/semantics/article