Ca2+ Influx and Tyrosine Kinases Trigger Bordetella Adenylate Cyclase Toxin (ACT) Endocytosis. Cell Physiology and Expression of the CD11b/CD18 Integrin Major Determinants of the Entry Route

Humans infected with Bordetella pertussis, the whooping cough bacterium, show evidences of impaired host defenses. This pathogenic bacterium produces a unique adenylate cyclase toxin (ACT) which enters human phagocytes and catalyzes the unregulated formation of cAMP, hampering important bactericidal functions of these immune cells that eventually cause cell death by apoptosis and/or necrosis. Additionally, ACT permeabilizes cells through pore formation in the target cell membrane. Recently, we demonstrated that ACT is internalised into macrophages together with other membrane components, such as the integrin CD11b/CD18 (CR3), its receptor in these immune cells, and GM1. The goal of this study was to determine whether ACT uptake is restricted to receptor-bearing macrophages or on the contrary may also take place into cells devoid of receptor and gain more insights on the signalling involved. Here, we show that ACT is rapidly eliminated from the cell membrane of either CR3-positive as negative cells, though through different entry routes, which depends in part, on the target cell physiology and characteristics. ACT-induced Ca2+ influx and activation of non-receptor Tyr kinases into the target cell appear to be common master denominators in the different endocytic strategies activated by this toxin. Very importantly, we show that, upon incubation with ACT, target cells are capable of repairing the cell membrane, which suggests the mounting of an anti-toxin cell repair-response, very likely involving the toxin elimination from the cell surface.

Genero eta nazio identitateak Katalina Eleizegiren antzezlanetan

383 p. ; P.315-383: "Erauso Kateriñe" antzezlanaren edizioa dago.

Corona y poderes urbanos en la Cornisa Cantábrica, siglos XVI y XVII

[ES]La formación de oligarquías municipales es un fenómeno que, arrancando de la Baja Edad Media, va alcanzando madurez durante la época de los Austrias. En ese tiempo fue posible hacer compatible el crecimiento del poder real con el mantenimiento del poder de las oligarquías en su territorio. En el presente trabajo se analizan el desarrollo y las peculiaridades de este proceso en las principales villas de la Cornisa Cantábrica.

Factors associated with chronic pain in patients with bipolar depression: a cross-sectional study

Background: While pain is frequently associated with unipolar depression, few studies have investigated the link between pain and bipolar depression. In the present study we estimated the prevalence and characteristics of pain among patients with bipolar depression treated by psychiatrists in their regular clinical practice. The study was designed to identify factors associated with the manifestation of pain in these patients.- Methods:Patients diagnosed with bipolar disorder (n=121) were selected to participate in a cross-sectional study in which DSM-IV-TR criteria were employed to identify depressive episodes. The patients were asked to describe any pain experienced during the study, and in the 6 weeks beforehand, by means of a Visual Analogical Scale (VAS).- Results: Over half of the bipolar depressed patients (51.2%, 95% CI: 41.9%–60.6%), and 2/3 of the female experienced concomitant pain. The pain was of moderate to severe intensity and prolonged duration, and it occurred at multiple sites, significantly limiting the patient’s everyday activities. The most important factors associated with the presence of pain were older age, sleep disorders and delayed diagnosis of bipolar disorder.- Conclusions: Chronic pain is common in bipolar depressed patients, and it is related to sleep disorders and delayed diagnosis of their disorder. More attention should be paid to study the presence of pain in bipolar depressed patients, in order to achieve more accurate diagnoses and to provide better treatment options.

Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice

Background: Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the Picornaviridae family. Due to its peculiar genome organization and characteristics of the per os viral transmission route, dicistroviruses make good candidates for use as biopesticides. Triatoma virus (TrV) is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of the human trypanosomiasis disease called Chagas disease. TrV was postulated as a potential control agent against Chagas' vectors. Although there is no evidence that TrV nor other dicistroviruses replicate in species outside the Insecta class, the innocuousness of these viruses in humans and animals needs to be ascertained. Methods: In this study, RT-PCR and ELISA were used to detect the infectivity of this virus in Mus musculus BALB/c mice. Results: In this study we have observed that there is no significant difference in the ratio IgG2a/IgG1 in sera from animals inoculated with TrV when compared with non-inoculated animals or mice inoculated only with non-infective TrV protein capsids. Conclusions: We conclude that, under our experimental conditions, TrV is unable to replicate inmice. This study constitutes the first test to evaluate the infectivity of a dicistrovirus in a vertebrate animal model.

Bizikletako aurreko abatzaren mekanizaziorako cam bidezko programazioa

[EU]Lan honek bizikletako aurreko gurpileko abatz baten CAM edo CNC programazioa egitean datza, honen bidez erremintaren ibilbidea deskribatuz piezaren CAD eredutik abiatuz. Hasteko, zenbakizko kontrolak izan duen eboluzioa eta garrantzia azaltzen da. Ondoren, abatzaren funtzionamenduaren deskribapena egin da, bere osagaiak eta izan behar dituen ezaugarriak aztertuz. Gainera, fabrikazio prozesu ezberdinen analisia egin da, bakoitzaren abantaila eta desabantailak azpimarratuz. Azkenik, zenbakizko kontrolaren inguruan bereganatutako ezagutzak aplikatu dira CAM programa batean abatz baten mekanizazioa programatuz. Honez gain, erabilitako CAM programaren erabilera azaltzen da.

Elejabarri eskolako aldaketa prozesua, Ikas Komunitate proiektuaren bitartez

[eus] Burututako ikerketa honen bidez, Ikas Komunitateen proiektuak, Elejabarri eskolan izan duen eragina ikustea bilatu da, eskolan sortu den aldaketa prozesua aztertuz. Horretarako, metodologia kualitatiboa erabili da, elkarrizketa erabiliz, beharrezko datu eta informazio guztiak lortzeko asmoz. Egindako elkarrizketa hauen bidez, ikusi da, Ikas Komunitateen proiektuari esker, aldaketa nabaria egon dela eskolan, aztertutako aspektu edo kategoria guztietan (absentismo maila; ikasle kopurua eta hauen ezaugarriak; ikasleen jarrerak; ikasleen, irakasleen eta familien arteko harremanak eta ikasleek lortutako emaitzak). Hori dela eta, Ikas Komunitateen proiektuak, eskolan eragin handia izan duela ondorioztatu da.