Increased opioid dependence in a mouse model of panic disorder

[EN] Panic disorder is a highly prevalent neuropsychiatric disorder that shows co-occurrence with substance abuse. Here, we demonstrate that TrkC, the high-affinity receptor for neurotrophin-3, is a key molecule involved in panic disorder and opiate dependence, using a transgenic mouse model (TgNTRK3). Constitutive TrkC overexpression in TgNTRK3 mice dramatically alters spontaneous firing rates of locus coeruleus (LC) neurons and the response of the noradrenergic system to chronic opiate exposure, possibly related to the altered regulation of neurotrophic peptides observed. Notably, TgNTRK3 LC neurons showed an increased firing rate in saline-treated conditions and profound abnormalities in their response to met5-enkephalin. Behaviorally, chronic morphine administration induced a significantly increased withdrawal syndrome in TgNTRK3 mice. In conclusion, we show here that the NT-3/TrkC system is an important regulator of neuronal firing in LC and could contribute to the adaptations of the noradrenergic system in response to chronic opiate exposure. Moreover, our results indicate that TrkC is involved in the molecular and cellular changes in noradrenergic neurons underlying both panic attacks and opiate dependence and support a functional endogenous opioid deficit in panic disorder patients.

SU-8 microprobes for biomedical applications

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La serigrafía de áridos con vehículos aglutinantes de base acuosa

330 p.

Formas básicas de representación y ejercicios de aprendizaje en dibujo (Un estudio basado en la observación de un grupo de alumnos adultos)

199 p. : il.

Eskolako eta Etxeko Azentua

Lan honetan hamar neska-mutil gazte ondarrutarren azentu ereduaren ezaugarri nagusiak aztertzen dira. Alde batetik, beraien ama hizkuntzaren eredua, hemen etxeko eredutzat aurkezten da, eta bestetik, euskara estandarra edo eskolakoa. Helburua, eredu bi hauek zeharo ezberdinak izateaz gain, bi hizkuntza ezberdin bezala funtzionatzen dutela frogatzea da. Horretarako, izen eta adjektiboen zerrenda bat egin eta azentuari zer gertatzen zaion aztertu da. Ondoren, smartphone batez baliatuz informanteak grabatu eta hauen transkripzioak egin dira. Azkenik, emaitzak analizatu ondoren, hasierako helburua betetzen dela ikusten da, hots, eredu biak ezberdinak direla. Ondorioz, eskolarako proposamenak sortzea ezinbestekoa da.

Fast and Efficient Neural Conversion of Human Hematopoietic Cells

Neurons obtained directly from human somatic cells hold great promise for disease modeling and drug screening. Available protocols rely on overexpression of transcription factors using integrative vectors and are often slow, complex, and inefficient. We report a fast and efficient approach for generating induced neural cells (iNCs) directly from human hematopoietic cells using Sendai virus. Upon SOX2 and c-MYC expression, CD133-positive cord blood cells rapidly adopt a neuroepithelial morphology and exhibit high expansion capacity. Under defined neurogenic culture conditions, they express mature neuronal markers and fire spontaneous action potentials that can be modulated with neurotransmitters. SOX2 and c-MYC are also sufficient to convert peripheral blood mononuclear cells into iNCs. However, the conversion process is less efficient and resulting iNCs have limited expansion capacity and electrophysiological activity upon differentiation. Our study demonstrates rapid and efficient generation of iNCs from hematopoietic cells while underscoring the impact of target cells on conversion efficiency.

Charge and spin transport in graphene devices

170 p.

Paper Art. Fibras papeleras en la Creación Artística. Producción y Edición

Nivel educativo: Grado. Duración (en horas): De 21 a 30 horas