A histopathologic evaluation of gross lesions excised from commercially important North Altantic marine fishes

Histopathologic studies of lesions found in commercially important North Atlantic marine fishes are uncommon. As part of a comprehensive Northeast Fisheries Center program ("Ocean Pulse") to evaluate environmental and resource health on the U.S. Continental Shelf from Cape Hatteras to Nova Scotia, grossly visible lesions of the gills, integument, muscle, and viscera of primarily bottom-dwelling fishes were excised and examined using light microscopy. Several gadid and pleuronectid fishes accounted for most of the lesions observed. Most pathological examinations were incidental to samples taken for age and growth determination and evaluation of predator/prey relationships. Several gadids, with either gill, heart, or spleen lesions, were sampled more intensively. Gill lesions principally affected gadids and were caused by either microsporidans or an unidentified oocyte-like cell. The majority of gastrointestinal lesions consisted of encapsulated or encysted larval worms or microsporidan-induced cysts. Few heart lesions were found. Integumental lesioos included ulcers, lymphocystis, and trematode metacercariae. Liver lesions almost always consisted of encapsulated or encysted larval helminths. Necrotic granulomata were seen in muscle and microsporidan-induced granulomata in spleen. Although not numerous, histologically interesting lesions were noted in integument, heart, liver, spleen, and muscle of several fish species. Histologic study of tissues excised from a variety of demersal and pelagic fishes from the eastern North Atlantic (France, Germany, Spain) revealed assorted integumental, renal, hepatic, and splenic lesions. Small sample size and non-random sampling precluded obtaining a meaningful quantitative estimate of the prevalence of the observed lesions in the population at risk; however, a useful census has been made of the types of lesions present in commercially important marine fishes. (PDF file contains 20 pages.)

A High-Speed Fish Evisceration System (FES) for Bycatch and Underutilized Fish Stocks

Development of a high-speed and high-yield water-powered fish evisceration system (FES) to efficiently preprocess small fish and bycatch for producing minced fish meat is described. The concept of the system is propelling fish in a stream of water through an arrangement of cutting blades and brushes. Eviscerated fish are separated from the viscera and water stream in a dual screen rotary sieve. The FES processed head off fish, weighing 170–500 g, at the rate of 300 fish/min when used with an automatic heading machine. Yields of mince produced from walleye pollock, Theragra chalcogramma; and Pacific whiting, Merluccius productus; processed by the FES ranged between 43% and 58%. The maximum yield of minced muscle from fish weighing over 250 g was 52%, and the yield of 250 g was 58%. Test results indicated that surimi made from minced meat recovered from fish processed with the FES was comparable in quality to commercial grade surimi from conventional systems. Redesigned for commercial operation in the Faeroe Islands (Denmark), the system effectively processed North Atlantic blue whiting, Micromesistius poutassou, with an average weight of 110 g at a constant rate of 500–600 fish/min, producing deboned mince feeding a surimi processing line at a rate of 2.0 t/h. Yields of mince ranged from 55% to 63% from round fish. Surimi made from the blue whiting mince meat produced by the FES was comparable to surimi commercially produced from blue whiting by Norway and France and sold into European markets.

Migration patterns of young Pacific bluefin tuna (Thunnus orientalis) determined with archival tags

We investigated the migration and behavior of young Pacific bluefin tuna (Thunnus orientalis) using archival tags that measure environmental variables, record them in memory, and estimate daily geographical locations using measured light levels. Swimming depth, ambient water temperature, and feeding are described in a companion paper. Errors of the tag location estimates that could be checked were –0.54° ±0.75° (mean ±SD) in longitude and –0.12° ±3.06° in latitude. Latitude, estimated automatically by the tag, was problematic, but latitude, estimated by comparing recorded sea-surface temperatures with a map of sea-surface temperature, was satisfactory. We concluded that the archival tag is a reliable tool for estimating location on a scale of about one degree, which is sufficient for a bluefin tuna migration study. After release, tagged fish showed a normal swimming behavioral pattern within one day and normal feeding frequency within one month. In addition, fish with an archival tag maintained weight-at-length similar to that of wild fish; however, their growth rate was less than that of wild fish. Of 166 fish released in the East China Sea with implanted archival tags, 30 were recovered, including one that migrated across the Pacific Ocean. Migration of young Pacific bluefin tuna appears to consist of two phases: a residency phase comprising more than 80% of all days, and a traveling phase. An individual young Pacific bluefin tuna was observed to cover 7600 km in one traveling phase that lasted more than two months (part of this phase was a trans-Pacific migration completed within two months). Many features of behavior in the traveling phase were similar to those in the residency phase; however the temperature difference between viscera and ambient temperature was larger, feeding was slightly more frequent, and dives to deeper water were more frequent.

Swimming depth, ambient water temperature preference, and feeding frequency of young Pacific bluefin tuna (Thunnus orientalis) determined with archival tags

We investigated the migration and behavior of young Pacific Bluefin tuna (Thunnus orientalis) using archival tags. The archival tag measures environmental variables, records them in its memory, and estimates daily geographical locations based on measured light levels. Of 166 archival tags implanted in Pacific bluefin tuna that were released at the northeastern end of the East China Sea from 1995 to 1997, 30 tags were recovered, including one from a fish that migrated across the Pacific. This article describes swimming depth, ambient water temperature, and feeding frequency of young Pacific bluefin tuna based on retrieved data. Tag performance, effect of the tag on the fish, and horizontal movements of the species are described in another paper. Young Pacific bluefin tuna swim mainly in the mixed layer, usually near the sea surface, and swim in deeper water in daytime than at nighttime. They also exhibit a pattern of depth changes, corresponding to sunrise and sunset, apparently to avoid a specific low light level. The archival tags recorded temperature changes in viscera that appear to be caused by feeding, and those changes indicate that young Pacific bluefin tuna commonly feed at dawn and in the daytime, but rarely at dusk or at night. Water temperature restricts their distribution, as indicated by changes in their vertical distribution with the seasonal change in depth of the thermocline and by the fact that their horizontal distribution is in most cases confined to water in the temperature range of 14−20°C.

Opportunistic feeding of longhorn sculpin (Myoxocephalus octodecemspinosus): Are scallop fishery discards an important food subsidy for scavengers on Georges Bank?

There has been much recent interest in the effects of fishing on habitat and non-target species, as well as in protecting certain areas of the seabed from these effects (e.g. Jennings and Kaiser, 1998; Benaka, 1999; Langton and Auster, 1999; Kaiser and de Groot, 2000). As part of an effort to determine the effectiveness of marine closed areas in promoting recovery of commercial species (e.g. haddock, Melanogrammus aegelfinus; sea scallops, Placopecten magellanicus; yellowtail flounder, Limanda ferruginea; cod, Gadus morhua), nontarget species, and habitat, a multidisciplinary research cruise was conducted by the Northeast Fisheries Science Center (NEFSC), National Marine Fisheries Service. The cruise was conducted in closed area II (CA-II) of the eastern portion of Georges Bank during 19–29 June 2000 (Fig. 1). The area has historically produced high landings of scallops but was closed in 1994 principally for groundfish recovery (Fogarty and Murawski, 1998). The southern portion of the area was reopened to scallop fishing from 15 June to 12 November 1999, and again from 15 June to 15 August 2000. While conducting our planned sampling, we observed scallop viscera (the noncalcareous remains from scallops that have been shucked by commercial fishermen at sea) in the stomachs of several fish species at some of these locations, namely little skate (Raja erinacea), winter skate (R. ocellata), red hake (Urophycis chuss), and longhorn sculpin (Myoxocephalus octodecemspinosus). We examined the stomach contents of a known scavenger, the longhorn sculpin, to evaluate and document the extent of this phenomenon.

Palatability of tissues in Holothuria leucospilota (Brandt) from central west coast of India

The palatability of various organs (body wall, cuvierian gland, viscera, longitudinal muscle bands and gonads) of sea cucumber Holothuria leucospilota (Brandt) was studied by feeding experiments, performed on a freshwater fish Sarotherodon mossambicus and a marine fish Therapon jarbua. The result shows that the food pellets of the body wall were less toxic and more palatable than the gonads, viscera and cuvierian gland (p<0.001).

Effects of supplementary feeds on growth and survival of freshwater giant prawn (Macrobrachium resenbergii [i.e. rosenbergii] deMan)

Highest growth of prawn was obtained with Feed B (743 kg/ha) with highest survival rate (60.88%) followed by Feed A where production and survival rate was 659 kg/ha and 53.50%, respectively. Feed A contained 30% dry ground cow viscera, 40% oil cake, 20% rice-bran and 10% heat bran. Feed conversion ratios were found to be 7.60:1 for Feed A and 6.46:1 for Feed B, which indicated that Feed B was more efficiently utilized by the prawn than Feed A. Statistical analysis revealed that the differences in production of prawns among the treatments were highly significant (P< 0.01).

Studies on the preparation of fish silage. 2. Rate of liquefaction in different parts of silver belly

An experiment was undertaken to determine from which part of silver belly (Leiognathus splendens) carcasses originate the autolytic enzymes responsible for liquefaction of silage. Findings show that it is important to leave head and viscera present in order to get a satisfactory liquefaction in silage prepared from silver belly using 3.5% formic acid. The storage life of silage produced from various parts of the fish carcasses is also discussed.

On the little known species of threadfin breams Nemipterus mesoprion (Bleeker) and Nemipterus delagoae (Smith)

Among the fish species of the family Nemipteridae, the two little known species, namely, Nemipterus mesoprion (Bleeker) and Nemipterus delagoae (Smith) are recorded from the Cochin waters. Nemipterus mesoprion is a new distributional record from the west coast of India. N. delagoae is described with adequate numbers of specimen for the first time from Indian waters. Moreover, the results of the present study show that the colour of the viscera, the number of pyloric caeca and the gill rakers can also be used for the diagnosis of the species of the genus Nemipterus. The affinity of the above species with other related species of the genus Nemipterus and their geographical distribution are presented.

Utilisation of squid waste as meal

The squid waste which includes head, tentacles, viscera, fin, skin and pen amounts to 52% of the whole weight and is discarded at present. A method has been worked out for the conversion of squ.id waste into meal. The waste is boiled in salt solution (2%)/salt (2%) and alumn (0.5%) solution/and water for two minutes, drained and dried. All the dried samples including the control (dried without blanching) were analysed for physical and biochemical changes. Blanching reduced the yield but the product could be dried in a shorter period. The volatile bases were reduced significantly and the colour was improved. Blanching made pulverisation of the dried product easy. Pulverisation before drying yielded a granular product.