Development of cell culture from caudal fin and heart of Tor tor (Hamilton-Buchanan)

Tor mahseer (Tor tor), possess high commercial and recreational value as they are potential game as well as food fish of India. Two cell culture systems were developed from fin and heart of T. tor (Hamilton-Buchanan). The explants excised aseptically from fingerling of T. tor were cultured in Leibovitz-15 (L-15) medium with 20% fetal bovine serum (FBS). Radiation of cells started after 72 hours and 48 hours of explant attachment from caudal fin and heart respectively. Confluent monolayer of cells with heterogeneous morphology around fin explants was observed after 7-10 days, where as a homogenous confluent layer of fibroblastic cells from heart explant was observed after 12-13 days. The establishment of cell culture systems from different organs and tissues of commercial important species would facilitates in vitro research.

Caudal neurosecretory system of four hill stream fishes of India

Caudal neurosecretory system is an additional neuroendocrine system found in fishes. Great variation has been observed among different groups of fishes, so far its organization is concerned. Much work has been undertaken on the caudal neurosecretory system of elasmobranchs and teleosts. Large size scattered Dahlgren cells in the posterior end of spinal cord, corresponding to last few vertebrae, with long running axon process and a neurohaemal organ the urophysis are the characteristic features of the system. Although thoroughly investigated in fresh water carps, no work is reported in hill-stream fishes. In an attempt to investigate structure and organization of caudal neurosecretory system in hill-stream fishes, present investigation was undertaken in four hill-stream fish of Indian freshwater namely, Barilius bendelensis, Garra gotyla, Schizothorax plagiostomus and Tor tor. The organization of this system in hill-stream fishes was found to be quite different from that observed in fresh water carps. It displays an organization which is more close to the organization of caudal neurosecretory system observed in elasmobranchs. The features are described and discussed.

Bacterial and pathological study of caudal fin rot in brood stocks of Salmo trutta caspius from the propagation and breeding center of Shahid Bahonar of Kelardasht region

In order to study caudal fin rot with emphasis on Aeromonas hydrophila and Pseudomonas fluorescens in Salmo trutta caspius from the salmonids propagation and breeding center of Shahid Bahonar of kelardasht region, One hundred and eighty brood stocks having fin damage symptoms were chosen. Two bacterial samples from each fish were cultured on Aeromonas and Pseudomonas specific media. Biochemical tests, API2OE identification system and antibiogram test using six antibiotic disks were performed for diagnosing isolates bacteria and finding suitable antibiotic. Thirty samples from caudal fin of damaged fishes were fixed in 10% formalin and 51.tm microscopic sections were prepared using standard scatological methods and then stained by Haematoxylin-Eosin staining method to observe the pathological changes and also Maccallum-Goodpasture staining method to observe the bacterial colonies. In second stage of the study, bacterial samples were taken from thirty brood stocks using similar method at the first stage of sampling. For isolation and biochemical diagnosis of Aeromonas and Pseudormonas genus, the samples were analyzed by molecular research included PCR amplification (using 16S rDNA genes of the genus pseudomonas and 16S-23S rDNA intergenic spacer of the genus Aeromonas) and restriction analysis by four restriction enzymes for each genus. The results of biochemical tests showed that isolated bacteria were belonged to Aeromonas caviae and Aeromonas hydrophila (subspecies anaerogenes), Pseudomonas fluorescens, Pseudomonas putida and Pseudomonas alcaligenes while the results of API2OE identification system showed that the isolated bacteria belonged to Aeromonas hydrophila, Pseudomonas fluorescens, Pseudomonas putida and Pseudomonas aeruginosa. Restriction analysis of Aeromonas samples with Hin6l, Csp6I, Taql, and Tasl revealed three samples were different from others while restriction analysis of Pseudomonas samples with Alul, Hinfl, Rsal, and Trull showed at least five species or biovars. The results of antibiogram test showed all Aeromonas samples were sensitive to Trimethoprim, Chloramphenicol and Nitrofurazone, mostly to Nalidixic acid and Chloramphenicol, while most of samples were resistant to Erythromycin and Oxytetracycline. Pseudomonas samples were only sensitive to Nitrofurazone and mostly resistant to Oxytetracycline, Nalidixic acid, Erythromycin, Trimethoprim and Chloramphenicol. The results of light microscope study showed hyperplasia and spongiosis of the malpigian cells of epidermis, increasing of melanin pigments underlying epidermis; sever necrosis in both epidermis and dermis and also sloughing the epidermis in some cases. Occurrence of clefts through the epithelium, neovascularization, hyperemia and mild inflammatory response in dermis and separation of the fin rays also were observed. No bacterial colonies were found in the sections.

Meristic variation in Sebastes (Scorpaenidae), with an analysis of character association and bilateral pattern and their significance in species separation

This report presents meristic data for nearly all of the known species of Sebasles. Rudimentary caudal ray counts tend to be higher in more active species. The number of caudal rays supported by the hypurals is consistently 14, whereas the number of branched caudal rays varies between 11 and 13. Vertebral counts and most fin-ray counts tend to be lower in species or populations in warmer latitudes, except for pectoral ray counts which tend to have an opposite geographic pattern. On the basis of the small magnitude of meristic and morphometric differences and the lack of other differences between northern and southern samples of "Sebasles caurinus," Sebaslichlhys vexillaris Jordan and Gilbert is regarded as a junior synonym of Sebasles caurinus Richardson. The patterns of bilateral variation in paired meristics are analyzed and their mechanism discussed. The frequency distribution of pectoral ray counts in their right-left combination is shown to be useful in species separation. No association was found between any combination of two meristic features in any species. The author proposes that intrasample associations between meristic features are evidence of sampling heterogeneity. (PDF file contains 21 pages.)

Eggs, Larvae and Young of The Striped Bass, Roccus saxatilis

Detailed descriptions of the early development of the striped bass, Roccus saxitilis (Walbaum), with emphasis on variation in size and morphology, sequence of fin formation, changes in body form, and attainment of the full complement of maristic numbers, are presented and illustrated for the first time. The egg is spherical, transparent, non-adhesive and relatively large. It is pelagic and buoyant, although it sinks in quiet fresh water. When unfertilized, it averages 1.3 mm, in diameter, but is 3.4 mm. when fertilized and water-hardened. The granular yolk sac, green when alive and whitish-yellow when preserved, averages 1.2 mm., and the single amber-colored oil globule is about 0.6 mm. in diameter. Newly hatched striped bass prolarvae, which range from 2.9-3.7 mm. in total length, are relatively undeveloped and nearly transparent, with no mouth opening, unpigmented eyes, and a greatly enlarged yolk sac with the large oil globule projecting beyond the head. When 5-6 mm. long the yolk sac and oil globule are assimilated and the postlarvae I show advanced development of the internal anatomy. Although the fish is still transparent, scattered melanophores are found on the head and body and chromatophores in the eyes and the ventro-posterior edge of the body. Postlarvae transform to young between 7 and 10 mm. in length when the finfolds are lost except in the dorsal, anal and caudal regions. The largest fish in this group possess a well-formed skeleton with a full complement of 25 vertebrae. Between 10 and 20 mm. in length all fish are fully transformed, muscular tissue renders most of the internal structure obscure, and the myotomes, which generally correspond in number with the vertebrae, are no longer visible. At fish lengths of 20-30 mm. scales are found on all specimens, and with the exception of the pectoral fin-rays, a full complement of meristic structures is present in all other fins. At this stage the body is pigmented uniformly with small spots. Linear regressions between several dependent variables and the , independent variable of standard length indicate that the rate of development of head, eye. and snout to anus lengths is proportional to the length of the larvae and young. Body depth and standard length are non-linear among newly-hatched larvae. Hatchery-reared striped bass demonstrated a slow rate of growth, and were regarded as "stunted," when compared to growth rates observed in another study and field collections. Observations were also made on abnormal eggs and teratological larvae and young. Blue-sac disease is tentatively identified and described for the first time in larvae and pugnosed larvae and young are also described for the first time in striped bass.

Larval development of the spotfin tonguefish (Symphurus oligomerus Mahadeva and Munroe, 1990) (Pleuronectiformes: Cynoglossidae) from the Gulf of California, Mexico

Larval and early juvenile stages of Symphurus oligomerus are described from 24 specimens from the Gulf of California. Meristic features were 48 – 49 total vertebrae, 87–94 dorsal-fin rays, 73–77 anal-fin rays, 12 caudal-fin rays, and five hypural bones. Seven larvae and one juvenile were cleared and stained to obtain the pterygiophore formula (1-3-2-2-2) that confirmed the identification of S. oligomerus. The pigment pattern from preflexion to juvenile stage consists of three bands on the dorsal margin and two bands on the ventral margin formed by star-shaped melanophores on the left side of the body. The intestine in preflexion to postflexion larvae forms an abdominal projection that ends in a short conical appendix. The intestine is supported by three cartilaginous struts; larvae with these physical attributes are called exterilium larvae. Preflexion larvae have two elongated dorsal-fin rays, and in flexion to postflexion larvae the second to the fourth dorsalfin rays are elongate. We found an apparent connection between the size at metamorphosis of the species of Symphurus and the depth distribution range of adults such that the fish species that metamorphose at a larger size have a deeper distribution as adults and exterilium larvae seem to correspond to species that have deeper distributions.

Description of early life history stages of the northern sculpin (Icelinus borealis Gilbert) (Teleostei: Cottidae)

Larvae of the genus Icelinus are collected more frequently than any other sculpin larvae in ichthyoplankton surveys in the Gulf of Alaska and Bering Sea, and larvae of the northern sculpin (Icelinus borealis) are commonly found in the ichthyofauna in both regions. Northern sculpin are geographically isolated north of the Aleutian Islands, Alaska, which allows for a definitive description of its early life history development in the Bering Sea. A combination of morphological characters, pigmentation, preopercular spine pattern, meristic counts, and squamation in later developmental stages is essential to identify Icelinus to the species level. Larvae of northern sculpin have 35–36 myomeres, pelvic fins with one spine and two rays, a bony preopercular shelf, four preopercular spines, 3–14 irregular postanal ventral melanophores, few, if any, melanophores ventrally on the gut, and in larger specimens, two rows of ctenoid scales directly beneath the dorsal fins extending onto the caudal peduncle. The taxonomic characters of the larvae of northern sculpin in this study may help differentiate northern sculpin larvae from its congeners, and other sympatric sculpin larvae, and further aid in solving complex systematic relationships within the family Cottidae.

Growth and maturity of salmon sharks (Lamna ditropis) in the eastern and western North Pacific, and comments on back-calculation methods

Age and growth estimates for salmon sharks (Lamna ditropis) in the eastern North Pacific were derived from 182 vertebral centra collected from sharks ranging in length from 62.2 to 213.4 cm pre-caudal length (PCL) and compared to previously published age and growth data for salmon sharks in the western North Pacific. Eastern North Pacific female and male salmon sharks were aged up to 20 and 17 years, respectively. Relative marginal increment (RMI) analysis showed that postnatal rings form annually between January and March. Von Bertalanffy growth parameters derived from vertebral length-at-age data are L∞ =207.4 cm PCL, k=0.17/yr, and t0=−2.3 years for females (n=166), and L∞ =182.8 cm PCL, k=0.23/yr , and t0=−1.9 years for males (n=16). Age at maturity was estimated to range from six to nine years for females (median pre-caudal length of 164.7 cm PCL) and from three to five years old for males (median precaudal length of 124.0 cm PCL). Weight-length relationships for females and males in the eastern North Pacific are W=8.2 × 10_05 × L2.759 –06 × L3.383 (r2 =0.99) and W=3.2 × 10 (r2 =0.99), respectively. Our results show that female and male salmon sharks in the eastern North Pacific possess a faster growth rate, reach sexual maturity earlier, and attain greater weight-at-length than their same-sex counterparts living in the western North Pacific.

A new species of western Atlantic lizardfish (Teleostei: Synodontidae: Synodus) and resurrection of Synodus bondi Fowler, 1939, as a valid species from the Caribbean with redescriptions of S. bondi, S. foetens (Linnaeus, 1766), and S. intermedius (Agassiz, 1829)

Western Atlantic synodontid species were studied as part of an ongoing effort to reanalyze Caribbean shorefish diversity. A neighbor-joining tree constructed from cytochrome c oxidase I (COI) data revealed 2 highly divergent genetic lineages within both Synodus intermedius (Agassiz, 1829) (Sand Diver) and S. foetens (Linnaeus, 1766) (Inshore Lizardfish). A new species, Synodus macrostigmus, is described for one of the S. intermedius lineages. Synodus macrostigmus and S. intermedius differ in number of lateral-line scales, caudal pigmentation, size of the scapular blotch, and shape of the anterior-nostril flap. Synodus macrostigmus and S. intermedius have overlapping geographic and depth distributions, but S. macrostigmus generally inhabits deeper water (>28 m) than does S. intermedius and is known only from coastal waters of the southeastern United States and the Gulf of Mexico, in contrast to those areas and the Caribbean for S. intermedius. Synodus bondi Fowler, 1939, is resurrected from the synonymy of S. foetens for one of the S. foetens genetic lineages. The 2 species differ in length and shape of the snout, number of anal-fin rays, and shape of the anterior-nostril flap. Synodus bondi and S. foetens co-occur in the central Caribbean, but S. bondi otherwise has a more southerly distribution than does S. foetens. Redescriptions are provided for S. intermedius, S. foetens, and S. bondi. Neotypes are designated for S. intermedius and S. foetens. A revised key to Synodus species in the western Atlantic is presented.

Nueva especie del género Mugil (Pisces: Mugilidae) de aguas cubanas.

A new species of mullet (Mugil) from Nipe Bay. North coast of Oriente Province, Cuba. The absence of axillary scale of pectoral fins, a very slender body and a notably elongated caudal peduncle are among the most diagnostic features of a new species.

Diel vertical migration of the bigeye thresher shark (Alopias superciliosus), a species possessing orbital retia mirabilia

The bigeye thresher shark (Alopias superciliosus, Lowe 1841) is one of three sharks in the family Alopiidae, which occupy pelagic, neritic, and shallow coastal waters throughout the altropics and subtropics (Gruber and Compagno, 1981; Castro, 1983). All thresher sharks possess an elongated upper caudal lobe, and the bigeye thresher shark is distinguished from the other alopiid sharks by its large upward-looking eyes and grooves on the top of the head (Bigelow and Schroeder, 1948). Our present understanding of the bigeye thresher shark is primarily based upon data derived from specimens captured in fisheries, including knowledge of its morphological features (Fitch and Craig, 1964; Stillwell and Casey, 1976; Thorpe, 1997), geographic range as far as it overlaps with fisheries (Springer, 1943; Fitch and Craig, 1964; Stillwell and Casey, 1976; Gruber and Compagno, 1981; Thorpe, 1997), age, growth and maturity (Chen et al., 1997; Liu et al., 1998), and aspects of its reproductive biology (Gilmore, 1983; Moreno and Moron, 1992; Chen et al., 1997).

Larval development of the southern sea garfish (Hyporhamphus melanochir) and the river garfish (H. regularis) (Beloniformes: Hemiramphidae) from South Australian waters

Larval development of the southern sea garfish (Hyporhamphus melanochir) and the river garfish (H. regularis) is described from specimens from South Australian waters. Larvae of H. melanochir and H. regularis have completed notochord flexion at hatching and are characterized by an elongate body with distinct rows of melanophores along the dorsal, lateral, and ventral surfaces; a small to moderate head; a heavily pigmented and long straight gut; a persistent pre-anal finfold; and an extended lower jaw. Fin formation occurs in the following sequence: caudal, dorsal and anal (almost simultaneously), pectoral, and pelvic. Despite the similarities between both species and among hemiramphid larvae in general, H. melanochir larvae are distinguishable from H. regularis by 1) having 58–61 vertebrae (vs. 51–54 for H. regularis); 2) having 12–15 melanophore pairs in longitudinal rows along the dorsal margin between the head and origin of the dorsal fin (vs. 19–22 for H. regularis); and 3) the absence of a large ventral pigment blotch anteriorly on the gut and isthmus (present in H. regularis). Both species can be distinguished from similar larvae of southern Australia (other hemiramphids and a scomberosocid) by differences in meristic counts and pigmentation.

Some aspects of the biology of Trypauchen vagina Bloch and Schneider of Bombay coast

Morphometrics of 14 metric and 6 meristic characters of Trypauchen vagina of Bombay coast is presented. Number of spines and rays of dorsal, anal and pectoral fins differ with earlier studies. Sexual dimorphism in the length of caudal and anal fins was observed. Length weight relationship of males and females have been worked out to be W = 0.00000 687L super(2.8440) and W = 0.00000 2747L super(3.0274) respectively. The fish is a prolific breeder and peak breeding activity occurs in December. The growth rate was found to be 15.20 mm/month.

Larval ontogeny of a percichthyid fish, Synagrops philippinensis (Günther) in Kagoshima Bay, southern Japan

The larval ontogeny of a developmental series (1.2-8.3mm body length, BL) of Synagrops philippinensis from Kagoshima Bay, southern Japan is described and illustrated. The yolk was completely absorbed in larva of ≥1.5 mm BL. Notochord flexion commenced at about 3.5mm BL and was completed by about 4.0-4.5mm BL. S. philippinensis larvae were distinguished from their congeners based on melanophore patterns, head spination and fin spines and rays. Larvae of 7.5-8.3 mm BL were characterized by anteriorly serrated pelvic spine, two anal spines, nine inner preopercular spines and no melanophore on lateral side of the caudal peduncle; 7.0 to 7.5mm BL larvae by the above characters except serration on pelvic spine; and yolk-sac, pre-flexion, flexing and post-flexion larvae up to 7.0mm BL by unique melanophores on lower lobe of pectoral finfold/fin.

Predatory behaviour of a snakehead fish (Channa striatus Bloch)

The predatory behaviour of a snake-head, Channa striatus (Bloch) on Labeo rohita fingerlings was studied in the laboratory. The study was conducted with six C. striatus (120 to 210 g and 22 to 28 cm) over 24h a day for 3 weeks. Three different sizes prey of large (2.00g and 5.8cm), medium (1.30g and 4.5cm) and small (0.72g and 3.5cm) were used for the first week and then medium size prey for 2nd and 3rd weeks. All the predators preferred eating the small group of L. rohita although all three size groups of L. rohita offered were available. It was found that the prey fishes remained together aside of the aquarium from the predator. Predator first targeted a prey, drove fast towards it, the prey tried to escape from the predator's attack using a specific route and finally the predator grasped the prey on head first and then engulfed. The handling time ranged between 45 and 50 sec. The time of peak feeding was found in the morning and in the evening of day. When 2 or 3 predators were kept in one aquarium, they engaged in fighting, head on, followed by an attack on the mouth region by the dominant one, and subsequently on the pectoral fin and caudal fin of the defeating one. After 2-3 days they became habituated to remain together and did not involve themselves in fighting.