Ensiled product from fish by microbial fermentation

Fish ensilage for animal feed stuff was prepared from Jew fish (Pseudosciaena spp.) and Silver bellies (Leiognathus spp.) by fermentation with pure culture of Lactobacillus piantarum NCIB 6105. The precooked ensiled product gave better product of fish silage (high content of lactic acid, about 5%). Protein Nitrogen content ranged between 1.76 to 1.94%. During storage for one year, the Protein Nitrogen loss was not significant. The material can be used as a supplemental animal ration.

Effects of iso-nitrogenous fertilizers as nutrient sources on carp polyculture in Bangladesh

A 120-day long experiment was conducted to find out the effects of urea plus triple super phosphate (UT), cow manure (CM) and poultry manure (PM) having iso-nil:rogen content on pond productivity and fish yield. Three fertilizer treatments, with three replicates each, were randomly assigned into nine earthen ponds of 100 m2 each. The stocking fish were rohu (Labeo ruhita), catla ( Catla catla) and mrigal ( Cirrhinus mrigala) in each treatment pond at the rate of 10000/ha with the ratio of 1:1:1. All ponds were fertilized fortnightly at the rate of 125 kg/ha urea plus 100 kg TSP/ha, 7000 kg/ha cow manure and 3500 kg/ha poultry manure for the treatment of UT, CM and PM, respectively, having an iso-nitrogen content of 56 kg in each. Though the physicochemical water quality parameters were more or less similar in all treatment ponds, the chlorophyll-a content and abundance of total plankton were significantly higher (P < 0.05) in the ponds receiving the treatment PM. Final growth as well as per unit production of fish was significantly higher (pnitrogen content is similar, in carp polyculture system under prevailing conditions.

Protein hydrolysate from miscellaneous fish

A method to prepare fish protein hydrolysate from miscellaneous fish obtained as by catch from shrimp trawlers is outlined. Effect of temperature and concentration of enzyme papain on the yield of hydrolysates has been determined. It is seen that within 30 min at 55°C and pH 6.5 fish proteins can be effectively solubilized, provided the nitrogen content of the enzyme (activity 10 units/mg enzyme) and substrate are maintained in the ratio 1:30. This hydrolysate possesses the best amino acid pattern compared to those obtained after hydrolysis for 60 to 180 min.

Studies on smoke curing of tropical fishes

Fresh mackerel (Rastrelliger kanagurta), catfish (Tachisurus dussumeri) and sole (Cynoglossus dubis) were gutted, cleaned, washed, brined, and smoked. Though it contributes to the quality of the smoked products, salt does not appear to prevent bacterial growth or spoilage at low concentration. In heavily salted products, salt is found to have a definite preservative action. Smoking lasted roughly 5 hrs for mackerel and 4 hrs for sole. Increasing the smoking time gave the product an unpleasant taste. Fish were then sun-dried to 20% moisture. The fish had been prepared in three groups, (1) turmeric-treated, (2) propionate-treated and control. Turmeric treated samples had a very attractive appearance, especially sole. The course of spoilage, as measured by following changes in total volatile nitrogen content, is tabulated. All controls were spoiled within 3 months; the others remained in good condition for 6 months. Turmeric is considered to be an ideal preservative.

Squilla protein: chemical composition and nutritive value

Protein extract prepared from squilla (Grato squilla nepa), a commercially unexploited crustacean, was analysed for crude protein and essential amino acids. All the essential amino acids except tryptophan and threonine were present in nutritionally adequate amounts. The protein was evaluated for its nutritional quality in respect of growth rate, protein efficiency ratio (PER) and liver nitrogen content by feeding on rats. Growth rates and protein efficiency ratios were similar in rats fed on casein, squilla protein and a combination of squilla protein and casein (1:1) diet. The weight of liver and kidneys were normal.

Ratio of natural isotopes of nitrogen. I. Primary results: soils of Dombes [Translation from: Revue d'Ecologie et de Biologie du Sol 14, 279-287, 1977]

Utilization of the heavy isotope of nitrogen as a tracer has found numerous applications in soil biology. It allows better definition of different stages of the nitrogen cycle, in particular the immobilization-mineralization cycle. In this work, the authors report the results of calculations of natural isotope ratios of nitrogen in samples of water, soil and vegetation prevailing in Dombes and discuss the possibilities of errors and coefficients of fractionation.

Do tissue carbon and nitrogen limit population growth of weevils introduced to control waterhyacinth at a site in the Sacramento-San Joaquin Delta, California?

Waterhyacinth (Eichhornia crassipes(Mart.) Solms), is a serious problem in the Sacramento Delta. Two weevil species (Neochetina bruchi Hustache and N. eichhorniae Warner) have been introduced as biological control agents. The purpose of this study was to test the hypothesis that nitrogen (N) in the tissue of waterhyacinth was not sufficient to support weevil growth and reproduction. Because it grows better on plants with high N content and because it has a greater impact on the growth of high N plants, N. bruchi may be a more effective biological control agent in the Sacramento Delta.

Reducing non-point DOC and nitrogen exports from rice fields: A pilot study and quantitative survey to determine the effects of different hydrologic management practices : Deliverable 15.3 Final Report

Rice cultivation at any level in the Sacramento–San Joaquin Delta (existing or expanded) compels the need to quantify surface and subsurface loads of dissolved organic carbon (DOC), disinfection byproduct precursors (DBPPs) and nitrogen. This information can be used to develop Best Management Practices (BMPs) to reduce export of these constituents in order to improve drinking water quality. Although rice cultivation in the Delta is relatively limited, several factors outside of this research could contribute to increased rice acreage in the Delta: • Recently developed rice varieties seem more suitable for the Delta climate than earlier varieties which required warmer conditions; • Previous economic analyses (Appendix A.10) suggest rice is more profitable than corn, a dominant land use in the Delta; • Recent studies on wetlands at Twitchell Island suggest rice production can help mitigate oxidative subsidence (Miller et al. 2000); • The different oxidative states that result from flooding in rice as compared to those found in crops that require drained soils may help control crop specific weeds and nematodes when rice is incorporated into a crop rotation; and • Providing flooded conditions during a greater part of the year than other crops may benefit water birds. ... (PDF contains 249 pages)

Analysis of soils and sediments for organic pollutants

20 samples of soil or sediment (7 of which were predominantly sand) from various locations were received for analysis of their content of organic pollutants. These analyses were performed using a capillary column gas chromatograph equipped with an electron impact (E.I.) mass spectrometer as detector and using computerised data storage. In addition to the target compounds, the full scan data were examined to determine the composition of natural organic products and a series of diagnostic fragment ions was used to search for additional anthropogenic products. Organic-rich environmental samples are notoriously difficult to analyse for pollutant organics owing to the presence of high concentrations of many natural organic compounds. A single procedure for extraction and clean-up was adopted. It was designed for chlorinated aliphatic and aromatic hydrocarbons and other pesticides containing acidic functional groups and was based on published methods for the determination of organic pollutants in soils and sediments. 4 soils and 2 sands showed levels of one or two groups of PCBs slightly in excess of the detection limit, one sample showed a similar level of 2,4-D and 3 samples contained dieldrin at or just above the detection limit.

Global transcriptional responses of the toxic cyanobacterium, Microcystis aeruginosa, to nitrogen stress, phosphorus stress, and growth on organic matter

Whole transcriptome shotgun sequencing (RNA-seq) was used to assess the transcriptomic response of the toxic cyanobacterium Microcystis aeruginosa during growth with low levels of dissolved inorganic nitrogen (low N), low levels of dissolved inorganic phosphorus (low P), and in the presence of high levels of high molecular weight dissolved organic matter (HMWDOM). Under low N, one third of the genome was differentially expressed, with significant increases in transcripts observed among genes within the nir operon, urea transport genes (urtBCDE), and amino acid transporters while significant decreases in transcripts were observed in genes related to photosynthesis. There was also a significant decrease in the transcription of the microcystin synthetase gene set under low N and a significant decrease in microcystin content per Microcystis cell demonstrating that N supply influences cellular toxicity. Under low P, 27% of the genome was differentially expressed. The Pho regulon was induced leading to large increases in transcript levels of the alkaline phosphatase phoX, the Pst transport system (pstABC), and the sphX gene, and transcripts of multiple sulfate transporter were also significantly more abundant. While the transcriptional response to growth on HMWDOM was smaller (5–22% of genes differentially expressed), transcripts of multiple genes specifically associated with the transport and degradation of organic compounds were significantly more abundant within HMWDOM treatments and thus may be recruited by Microcystis to utilize these substrates. Collectively, these findings provide a comprehensive understanding of the nutritional physiology of this toxic, bloom-forming cyanobacterium and the role of N in controlling microcystin synthesis.

Physical and chemical characteristics of major aquatic ecosystems in the Victoria and Kyoga lake basins

The physical-chemical characteristics of any aquatic ecosystem include pH, conductivity, and temperature, water transparency, nutrient and the chlorophyll-a levels. Physical and chemical factors of any ecosystem determine the type and quality of flora present in it and these forms the basis on which the system operates. The elements required in largest amounts for plant productions are carbon, phosphorus, nitrogen, and silicon, which is important for diatoms as a major component of the cell wall. Nutrients may limit algal productivity in the tropics despite the high temperature there allowing rapid nutrient recycling. Nutrients most likely to be limiting African lakes are nitrogen (Talling & Talling 1965; Moss 1969; Lehman & Branstrator 1993, 1994) and phosphorus (Melack.et al l982; Kalff 1983) while silicon may limit diatom growth (Hecky & Kilham 1988). The objective of the study is to investigate the impact of physical-chemical characteristics on the distribution and abundance of organisms in the major aquatic ecosystems.

Report of the baseline survey undertaken on the Chinese cage site in Napoleon Gulf, Northern Lake Victoria, 12 March 2012

The government of the People's Republic of China through a 2007 agreement with the Government of the Republic of Uganda, has establishment of an Agricultural Technology Demonstration Center (ATDC). The first phase covering the building of aquaculture infrastructure at Kajjansi ARDC is complete and the second operation phase has started in which facilities for cage culture have been set up in the Napoleon gulf, northern Lake Victoria near Jinja. The cage facility is aimed at boosting fish farming within the lake as a diversification to the traditional pond fish culture technology. NaFIRRI scientists as well as Chinese experts undertook a baseline survey in the chosen cage site on 12 March 2012. The survey covered determination of water depth, water transparency, measurement of selected physical-chemical parameters (temperature,dissolved oxygen, conductivity and pH; determination of the nutrient status and study of algae, invertebrate and fish communities at the site. Materials and methodologies used in the survey were based on the Standard Operating Procedures (SOPs) of NaFIRRI. The study area was divided into three study sites. Site 1 (upstream) was at 8.9 metre depth while site 2 (proposed cage site) and site 3 (downstream) were 6 and 4.3 metres deep respectively. Water transparency was lowest at site 1 (1.58 m) and highest at site 3 (1.64 m). Dissolved oxygen at the three sites ranged from 6.0 to 8 mg/I. Water temperature profiles fluctuated within narrow limits between 26.5 and 27.5 DC. Measurements of pH were between 7 (neutral) and 8 (alkaline) while electrical conductivity was between 98 and 101 uS/em. These observed physical-chemical parameters at the study site were considered suitable for cage fish rearing purposes. Nitrite-nitrogen levels varied within narrow limits from 0.043 to 0.0453 mgtl. Similarly, Ammonia-nitrogen varied between 0.015 and 0.0185 mg/1. Soluble reactive phosphorus (SRP) level was highest at site 3 (O.012mgll) compared to that at sites 1 and 2 (0.009mgll). Total suspended solids (TSS) were higher at site 1 (83.3mgll), thereafter decreasing to lower levels at sites 2 (24.8mgtl) and 3 (19.8mgl) respectively. The nutrient level results observed here all fall below the maximum permissible limits by NEMA and therefore the site is recommended for cage culture The algal community was constituted by four major groups: Blue greens,Greens, Cryptophytes, and Diatoms with blue greens as the common and dominant group. High algal biomass (19944961 ugtL) of the dominant blue green algae was observed at site 1 compared site 2 and 3 (58655.2 & 27487. 7 ugtL) respectively. Occurrence of toxicin producing algae: microsytis and cylindrospermopsis in the proposed cage area was considered to be of not much significance as their concentrations were below harmful levels. However, monitoring their presence, biomass and seasonality will be critical in order to follow when and where they occur and at what time of the year for ease of management of the cages