Correlation of cell numbers with catalase activities of pure strains of bacteria

An attempt was made to correlate the catalase production with the actual number of aerobic bacterial cells generating the enzyme: bacteria were obtained from the surface of marine fish. A linear correlation was found between the log of catalase activity and log of bacterial count in single culture.

Saline water intrusion from deep artesian sources in McGregor Isles area of Lee County, Florida

Upward leakage of saline water from an artesian aquifer below 1,500 feet has caused an increase in chloride concentration in the lower Hawthorn aquifer from less than 1,000 mg/1 (milligrams per liter) to values ranging from about 1,300 to 15,000 mg/1. Similarly the higher temperatures of the intruding water has caused an increase in water temperatures in the aquifer from 82"F to values ranging from 83 to 93"F. The intruding water moves upward either through the open bore hole of deep wells or test holes, or along a fault or fracture system, which has been identified in the area. From these points of entry into the lower Hawthorn aquifer, the saline water spreads laterally toward the south and southeast, but is generally confined to components of the fault system. The saline water moves upward from the lower Hawthorn aquifer into the upper Hawthorn aquifer through the open bore hole of wells, which connect the aquifers. This movement has resulted in an increase in chloride from less than 200 mg/1 in the unaffected parts of the upper Hawthorn aquifer to values commonly ranging from about 300 to more than 3,000 mg/1 in parts of the aquifer affected by upward leakage. The upper Hawthorn aquifer is the principal source of ground-water supply for public water-supply systems in western Lee County. Similar effects have been noted in the water-table aquifer, where chloride increased from less than 100 to concentrations ranging from about 500 to more than 5,000 mg/1. This was caused by the downward infiltration of water discharged at land surface from wells tapping the lower Hawthorn aquifer. The spread of saline water throughout most of the McGregor Isles area is continuing as of 1971. (40 page document)

Some ecological roles of Hippopotamus (Hippopotamus amphibious Linn. 1758) in fish production: possibilities for integrated fish-cum Agric production system

The common hippopotamus (Hippopotamus amphibious Linn. 1758) contributes to the productivity of aquatic systems where it lives. This paper reviews ecological roles of the hippo in this regard. Desk review of available literature information complemented with field observations were employed in the data collection. The ecological roles of the common hippopotamus being presented draw examples from East, West, Central and South African sub regions. The nutritional importance of the amphibious hippopotamus to rural communities was highlighted. In Southern Ethiopia, the Bodi, Bacha and Mura tribes eat hippo meat and this has led to severe hunting consequences on the wild populations of the animal. The important relationships between the hippopotamus and fish were presented. Hippopotamuses usually defecate in water and their excrements enrich the nutrients in the water resulting in favourable conditions for large fish populations. Some fish, including Labeo spp. were observed to feed on the micro-organisms and algae that grow on the skin of the hippotamus. A strong case was made for hippo-cum-fish integrated farm development in Nigeria based on ecological relationships so observed between the amphibious mammals and fish. This is one of the meeting points of fisheries and wildlife management that should be exploited for the benefits of the teeming Nigerian population

Effects of extracts of dried seeds of Toloache, Datura innoxia as anaesthesia on the African catfish Clarias gariepinus fingerlings

The effects of crude extract, pure extract, aqueous, fraction of pure and lipid fraction of pure extract of dried seeds of toloache. Datura innoxia as anaesthesia on the African catfish. Clarias gariepinus fingerlings were studied. The fish were exposed to various doses of the extract in aquaria tanks and the time taken for each fish to reach anaesthesia was recorded. The fish were anaesthetized up to 3.00g/l fingerlings reached anaesthesia is significantly (P<0.05) shorter time (1.004 minutes at 0.05gl) in pure unseparated extract than in crude extract (58.50 minutes at 3.00g/l concentrated). The time to reach anaesthesia decreased with an increase in concentration of the seed extract. Out the two fractions the lipid fraction had significantly (P>0.05) better anaesthetic on the fish. The control produced no observable anaesthetic effect on the fish within three hours. This suggests that the anaesthetizing active ingredent resided in the lipid fraction. All fish recovered from anaesthesia, swam and fed actively and no mortality was observed throughout the exposure period and thereafter. It is therefore recommended for use on C. gariepinus fingerlings

Radioautographic method of determination of number of bacteria in water-bodies [Translation from: Informatsionnyi Byulleten Biologiya Vnutrennikh Vod No.26, 65-67, 1975]

The radioautographic method of determination of the number of autotrophic microorganisms was initially suggested for counting methane-oxidizing bacteria. With the help of this method colonies of hydrogen-oxidizing bacteria are differentiated even more clearly from heterotrophic. Under laboratory conditions it was shown that colonies grown on membrane filters from a pure culture of thionic bacteria on a nutrient medium with radio- active carbonate, give better prints on film. This method was tested by the authors for determining the number of these bacteria in the meromictic Lake Vae de San Juan during the expedition to Cuba in the summer of 1973. The study showed that that the thionic bacteria are found throughout the pelagial. It proved that the thionic bacteria can be well considered in water-bodies by the radioautographic method.

Concerning the current conditions and transport of sand in the shallow water areas off the coast of the south-eastern North Sea [Translation from: Hamburger Kustenforschung 29 [pages 41-43 only], 1974]

With the aid of the German Research Association in the central programme 'Sand movements in the German coastal region', an investigation into the current conditions in the shallow water areas of the coasts of the south-eastern North Sea between Sylt and the Weser estuary was carried out by the author. Foundations of the work are 19 continuous current recordings in five profiles normal to the coast from years 1971 to 1973. Off the coasts of the south-eastern North Sea varying tidal currents impinge; they are currents whose directions may vary periodically through all points of the compass. They are caused by the circulating tides in the North Sea (Amphidromien). The turning flow movement experiences a deformation in the very shallow coastal waters, and as it happens the flow turning movement in the case of high tide continues right up onto the outer flats, while here and in the fore-lying shallow water areas around the time of low water (on account of the small depths of waters), there prevails a more variable current. A result of this hydrodynamical procedure is the development of counter currents. This partial translation of the original paper provides the summary of this study of of the mudflat areas between the Elbe and Weser.

Temporal Changes in a Tropical Nekton Assemblage and Performance of a Prawn Selective Gear

The temporal variation of components of a moderately diverse (H=1.46) tropical estuarine fish assemblage (long. 146°30'E, lat. 8°45'S) was directed by salinities that had been determined by local oceanographic and probably topographic conditions. For this assemblage, two types of intrayear component profiles are predicted. Pooled data (1988-91) reveal a large component of regular/resident species (43%) in an assemblage which has been under a narrow temperature regime «5T). These results facilitate a discussion on the relevance and usefulness of three hypotheses often cited in studies concerning species diversity and component characteristics of the subtropical/tropical coastal nonreef fish assemblages. Manifestations of the assemblage are reflected in catch composition and weights of 39 trials conducted for a selective prawning gear whose performance in bycatch reduction, mainly for finfishes, is judged by an index, E, we have previously proposed. This gear is capable of harvesting the prawn while conserving the demersal fish. Behavioral responses to netting of the prawns and the finfishes, especially the nearshore surface schoolers such as leiognathids, are discussed from several points of view. An adaptation in terms of group selection for leiognathids of their locking mechanism of median fin spines has been interpreted. For the purpose of bycatch reduction or E enhancement, suggestions for improvements in net design and trawl configuration by considering the behavioral features of fish are made. Our original formula of E is modified for general use. Bycatch problems in the regional prawn fisheries and their possible impacts on fishery planning and development in Papua New Guinea as a developing country are discussed. The gear tested may offer enormous ecological and economic benefits. The gear is multipurpose, extremely simple, and can also be used as a biological sampler.

The Feasibility of Enhancing Red Sea Urchin, Strongylocentrotus iranciscanus, Stocks in California: An Analysis of the Options

The California fishery for red sea urchins, Strongylocentrotus franciscanus, has undergone explosive growth in recent years and is approaching full exploitation. Thus, there is considerable interest in enhancing stocks to maintain a high rate of landings. Fishable stocks of red sea urchins in different areas appear to be limited at three stages in their life history: By the availability of larvae, by the survival of newly settled to mid-sized animals, and by the food available to support growth and reproduction of larger animals. Here I review other efforts, notably the extensive Japanese work, to enhance fishable stocks of benthic marine invertebrates, and consider the potential options for red sea urchins at different points of limitation. These include collecting or culturing seed for outplanting, physical habitat improvement measures, improving the food supply, and conservation measures to protect existing stocks until alternate methods are proven and in place. The options are compared in terms of biological feasibility, capital and labor requirements, and potential implications for change in the structure of the fishing industry.

Microbial community analysis of Acropora palamata mucus swabs, water and sediment samples from Hawksnest Bay, St. John, U.S. Virgin Islands

Colonies of the scleractinian coral Acropora palmata, listed as threatened under the US Endangered Species Act in 2006, have been monitored in Hawksnest Bay, within Virgin Islands National Park, St. John, from 2004 through 2010 by scientists with the US Geological Survey, National Park Service, and the University of the Virgin Islands. The focus has been on documenting the prevalence of disease, including white band, white pox (also called patchy necrosis and white patches), and unidentified diseases (Rogers et al., 2008; Muller et al., 2008). In an effort to learn more about the pathologies that might be involved with the diseases that were observed, samples were collected from apparently healthy and diseased colonies in July 2009 for analysis. Two different microbial assays were performed on Epicentre Biotechnologies DNA swabs containing A. palmata coral mucus, and on water and sediment samples collected in Hawksnest Bay. Both assays are based on polymerase chain reaction (PCR) amplification of portions of the small rRNA gene (16S). The objectives were to determine 1) if known coral bacterial pathogens Serratia marcescens (Acroporid Serratiosis), Vibrio coralliilyticus (temperature-dependent bleaching, White Syndrome), Vibrio shiloi (bleaching, necrosis), and Aurantimonas coralicida (White Plague Type II) were present in any samples, and 2) if there were any differences in microbial community profiles of each healthy, unaffected or diseased coral mucus swab. In addition to coral mucus, water and sediment samples were included to show ambient microbial populations. In the first test, PCR was used to separately amplify the unique and diagnostic region of the 16S rRNA gene for each of the coral pathogens being screened. Each pathogen test was designed so that an amplified DNA fragment could be seen only if the specific pathogen was present in a sample. A positive result was indicated by bands of DNA of the appropriate size on an agarose gel, which separates DNA fragments based on the size of the molecule. DNA from pure cultures of each of the pathogens was used as a positive control for each assay.

Elevating fishers of Dal Lake in Jammu and Kashmir

The deteriorating condition of one of the most magnificent lakes of India, the Dal Lake, has provoked a lot of research interest from the biological and ecological points of view. However, the impact of deterioration of the ecology on the inhabitant fisher folk around Dal Lake has not been given much importance. In this study, the three major beats of the lake, namely, Hazratbal, Nishat and Nehru Park, were surveyed, randomly taking 10% of the fishermen families. It was observed that the summer mean catch per day (5.18 ± 0.69 kg) was more than the mean winter catch per day (2.85 ± 0.40 kg). However, the total annual catch per person was only 1195.56 ± 211.63 kg. Moreover, marketing through middlemen, especially, during summer, was observed to be very low fetching in terms of mean price per kilogram fish, amounting to Rs 37.50 ± 2.54 for local fish and Rs 22.50 ± 2.88 for the carp. It was also observed that the fisher folk had borrowed a good amount of money for the repair and construction of their houses and boats. Therefore, on the whole, the fishing business around Dal Lake was observed to be under severe stress. In this context, this paper has come out with recommendations to uplift the socio-economic conditions of the fishers.

Status of fish broodstock management and seed production in Bangladesh

The study was conducted with the broad objectives to assess the existing situation of broodstock management and fish seed production in private fish seed farms in Bangladesh. The data were collected from 100 private hatcheries and 40 nurseries in seven upazilas under four districts. There was no shed in forty hatcheries and the owners faced many problems. Brood fish ponds were found suitable for rearing brood fish. About 66% of the hatchery owners collected brood fish from their own ponds and ponds of neighboring areas. Activities like pond preparation; manuring and supplementary feeding were done properly but stocking density of brood fish in 76% of the hatcheries was 3,000-7,000 kg/ha. Infection of argulosis was found in brood fish of 87% of the hatcheries. About 67% of the hatchery owners practiced inter-species crossing. Major problems faced by the hatchery owners were argulosis of brood fish, unavailability of pure brood stock, inadequate brood fish pond. The hatchery owners were found interested to find out the preventive measures of argulosis, develop pure brood stock of indigenous carp and import pure strain of exotic carp. According to the nursery operators, they cultivated hybrid fry because of high demand, rapid growth and good taste. Problems of using hatchery spawn as mentioned by the nursery operators were inbreeding, under sized and aged brood stock, stunted growth, physical deformities and high mortality of spawn due to unknown causes.

In vitro effects of fungicides on the fungus Haliphthoros philippinensis

Pure cultures of Haliphthoros philippinensis, isolated from infected Penaeus monodon larvae, were exposed for 24 hours to varying concentrations of antifungal agents. The efficiency of each agent to inhibit sporulation and mycelial growth was measured. Effects on P. monodon eggs and larvae were also investigated. It is concluded that preliminary bioassay of larval tolerance to the suggested effective doses should always be made prior to prophylaxix or therapeutic applications.

Species identification and authentication of some of the most important seafood products in Iranian markets using biotechnological methods based on DNA

This study describes the molecular identification of sixteen fish species present in processed products imported into Iran for human consumption. DNA barcoding using direct sequencing of about 650 bp of the mitochondrial Cytochrome Oxidase subunit I gene revealed incorrect labeling (31.25%). Substitution of fish species constitutes serious economic fraud, and our results increase concern regarding the trading of processed fish products in Iran from both health and conservation points of view.