The ionic composition of surface waters in the English Lake District, related to bedrock geology, with some singular facts and speculation on the existence of mineral-rich groundwaters

This article is based on a survey of tarns conducted mainly in the summers of 1983 to 1985, plus a survey made in the winter of 1985, in which streams were sampled on the wide variety of rock-types occurring on the fringes of the Lake District. Differences in composition of major ions and their concentrations in the surface waters of Cumbria reflect the complex geological structure of the region. At altitudes above 300 m, on Borrowdale Volcanics and Skiddaw Slates, surface waters are derived from atmospheric precipitation, with additional inputs of some ions - especially calcium and bicarbonate - from catchment rocks and soils. In some of the low-lying large lakes on the fringes of the central fells, water composition is also dominated by inputs from upper catchments; examples are Wastwater, Ullswater and Haweswater. However in other lakes there is evidence (Derwentwater and Bassenthwaite Lake) of inputs from saline groundwater.

The problem of the co-existence of Arctodiaptomus bacillifer (Koelb.), Acanthodiaptomus denticornis (Wierz.) and Heterocope saliens Lill [Translation from: Memorie dell'Istituto Italiano di Idrobiologia Dott.Marco de Marchi 8 81-94, 1954]

In freshwater environments of modest size and without notable ecological structure, there is usually present only one diaptomid species. When two or more diaptomid species are present in the same habitat, generally their body dimensions are distinctly different. There are only four examples of co-existence of Arctodiaptomus bacillifer (Koelb.) and Acanthodiaptomus denticornis (Wierz.) situated at higher altitudes alpine lakes. The article discusses the results of sampling in the summer of 1953 and the problem of the co-existence of Arctodiaptomus bacillifer, Acanthodiaptomus denticornis and Heterocope saliens.

Production of positive controls for calcivirus-specific PCR using recombinant baculiovirus technology

Recent advances in our knowledge of the genetic structure of human caliciviruses (HuCVs) and small round-structured viruses (SRSVs) have led to the development of polymerase chain reaction (PCR)-based molecular tests specific for these viruses. These methods have been developed to detect a number of human pathogenic viruses in environmental samples including water, sewage and shellfish. HuCVs and SRSVs are not culturable, and no animal model is currently available. Therefore there is no convenient method of preparing viruses for study or for reagent production. One problem facing those attempting to use PCR-based methods for the detection of HuCVs and SRSVs is the lack of a suitable positive control substrate. This is particularly important when screening complex samples in which the levels of inhibitors present may significantly interfere with amplificiation. Regions within the RNA polymerase regions of two genetically distinct human caliciviruses have been amplified and used to produce recombinant baculoviruses which express RNA corresponding to the calicivirus polymerase. This RNA is being investigated as a positive control substrate for PCR testing, using current diagnostic primer sets. Recombinant baculovirus technology will enable efficient and cost-effective production of large quantities of positive control RNA with a specific known genotype. We consider the development of these systems as essential for successful screening and monitoring applications.

Combating fish disease. An explanation of the controls on notifiable fish diseases in Great Britain and advice on steps to prevent the spread of disease

For many years action has been taken to prevent the introduction and spread of serious fish diseases in Great Britain. In 1993 national rules were replaced by European Union wide rules designed to promote trade within the single market while safeguarding those parts of the Union with a high fish health status - such as this country. This booklet details the checks and controls which are applied to prevent the spread of disease outbreaks in this country. One can see that different rules apply to different diseases, generally reflecting the severity and other characteristics of the disease. The booklet also tries to explain the diseases and helps to recognise symptoms. This booklet is split into three parts: Part 1 gives an overview of the controls; Part 2 gives details for each of the diseases; and Part 3 gives advice on some of the precautions you can take to guard against the spread of disease.