Marine eutrophication review. Part 1: Quantifying the effects of nitrogen enrichment on phytoplankton in coastal ecosystems; Part 2: Bibliography with abstracts

Professionals who are responsible for coastal environmental and natural resource planning and management have a need to become conversant with new concepts designed to provide quantitative measures of the environmental benefits of natural resources. These amenities range from beaches to wetlands to clean water and other assets that normally are not bought and sold in everyday markets. At all levels of government — from federal agencies to townships and counties — decisionmakers are being asked to account for the costs and benefits of proposed actions. To non-specialists, the tools of professional economists are often poorly understood and sometimes inappropriate for the problem at hand. This handbook is intended to bridge this gap. The most widely used organizing tool for dealing with natural and environmental resource choices is benefit-cost analysis — it offers a convenient way to carefully identify and array, quantitatively if possible, the major costs, benefits, and consequences of a proposed policy or regulation. The major strength of benefit-cost analysis is not necessarily the predicted outcome, which depends upon assumptions and techniques, but the process itself, which forces an approach to decision-making that is based largely on rigorous and quantitative reasoning. However, a major shortfall of benefit-cost analysis has been the difficulty of quantifying both benefits and costs of actions that impact environmental assets not normally, nor even regularly, bought and sold in markets. Failure to account for these assets, to omit them from the benefit-cost equation, could seriously bias decisionmaking, often to the detriment of the environment. Economists and other social scientists have put a great deal of effort into addressing this shortcoming by developing techniques to quantify these non-market benefits. The major focus of this handbook is on introducing and illustrating concepts of environmental valuation, among them Travel Cost models and Contingent Valuation. These concepts, combined with advances in natural sciences that allow us to better understand how changes in the natural environment influence human behavior, aim to address some of the more serious shortcomings in the application of economic analysis to natural resource and environmental management and policy analysis. Because the handbook is intended for non-economists, it addresses basic concepts of economic value such as willingness-to-pay and other tools often used in decision making such as costeffectiveness analysis, economic impact analysis, and sustainable development. A number of regionally oriented case studies are included to illustrate the practical application of these concepts and techniques.

Global transcriptional responses of the toxic cyanobacterium, Microcystis aeruginosa, to nitrogen stress, phosphorus stress, and growth on organic matter

Whole transcriptome shotgun sequencing (RNA-seq) was used to assess the transcriptomic response of the toxic cyanobacterium Microcystis aeruginosa during growth with low levels of dissolved inorganic nitrogen (low N), low levels of dissolved inorganic phosphorus (low P), and in the presence of high levels of high molecular weight dissolved organic matter (HMWDOM). Under low N, one third of the genome was differentially expressed, with significant increases in transcripts observed among genes within the nir operon, urea transport genes (urtBCDE), and amino acid transporters while significant decreases in transcripts were observed in genes related to photosynthesis. There was also a significant decrease in the transcription of the microcystin synthetase gene set under low N and a significant decrease in microcystin content per Microcystis cell demonstrating that N supply influences cellular toxicity. Under low P, 27% of the genome was differentially expressed. The Pho regulon was induced leading to large increases in transcript levels of the alkaline phosphatase phoX, the Pst transport system (pstABC), and the sphX gene, and transcripts of multiple sulfate transporter were also significantly more abundant. While the transcriptional response to growth on HMWDOM was smaller (5–22% of genes differentially expressed), transcripts of multiple genes specifically associated with the transport and degradation of organic compounds were significantly more abundant within HMWDOM treatments and thus may be recruited by Microcystis to utilize these substrates. Collectively, these findings provide a comprehensive understanding of the nutritional physiology of this toxic, bloom-forming cyanobacterium and the role of N in controlling microcystin synthesis.

Historical nitrogen and phosphorus loadings to the northern Gulf of Mexico

Primary productivity in many coastal systems is nitrogen (N) limited; although, phytoplankton productivity may be limited by phosphorus (P) seasonally or in portions of an estuary. Increases in loading of limiting nutrients to coastal ecosystems may lead to eutrophication (Nixon 1996). Anthropogenically enhanced eutrophication includes symptoms such as loss of seagrass beds, changes in algal community composition, increased algal (phytoplankton) blooms (Richardson et al. 2001), hypoxic or anoxic events, and fish kills (Bricker et al. 2003).

Physicochemical and biological cycles in a tide dominated, nitrogen-polluted temperate estuary

Spatio-temporal variations in the physicochemical and biological parameters in the Morlaix estuary on the Brittany coast of France were studied. Hydrographically, the estuary can be classified into 3 segments: the upper estuary where stratification always persists, the lower estuary where vertical homogeneity is permanent, and a middle estuary where there is a regular oscillation of stratification and homogeneity during every tidal cycle, stratification being associated with slack waters and homogeneity, with ebb and flood. Nitrogen pollution in the estuary is very intense.

The influence of nitrogen and phosphorus on the growth of a diatom Skeletonema costatum (Greville) Cleve

Nitrogen and phosphorus requirements of a chain-forming diatom, Skeletonema costatum (Greville) Cleve, collected from Yatsushiro Sea, Japan, were investigated in a laboratory culture experiment. Sodium nitrate and sodium glycerophosphate were used as nitrogen and phosphorus sources, respectively. Cultures were grown in modified Provasoli's ASP2NTA medium (Provasoli et al. 1957) at 25±1°C, light intensity 60 µE mˉ² secˉ¹ and photoperiod 12:12-h, L:D cycle. Optimum growth was observed at nitrate concentrations of 3-10 mglˉ¹ and phosphate concentrations of 1.5-15 mglˉ¹. Adequate growth was also found at the nitrate concentration of up to as high as 300 mglˉ¹. Significantly poorer growth was found at lower nitrate (<3.0 mglˉ¹) and higher phosphate (>15 mglˉ¹) concentrations. From the present study, it is concluded that S. costatum can grow well at wide ranges of nitrate concentrations but is sensitive to higher phosphate concentrations.

Analysis of lipid and lipid-fractions of some freshwater fishes and their inter-relationship

Two fish species each from carnivorous (Clarias batrachus, Channa punctatus), omnivorous (Cyprinus carpio, Cirrhinus reba), and plankton feeder (Catla catla, Labeo rohita) were collected from freshwater sources under natural habitat to study their total lipid (TL) and lipid-fractions. Significant relationship between these parameters was also worked out. The variation of total lipid and lipid-fractions in tissues of freshwater fishes were not significantly different (P>0.05). But a higher trend of total lipid and glyceride (TGL) contents were found in carnivores followed by omnivores and least in plankton feeders. The trend was reverse for total phospholipid, cholesterol and free fatty acids. TGL content in all class of fishes was significantly related with TL (P<0.01), phospholipid (PL) (P<0.001), cholesterol (P<0.05), free fatty acids (P<0.05) and monoglycerides (P<0.001). Similarly total lipid was linearly related with total glycerides (TL=-3.02 + 0.10 TGL) and phospholipid (TL=7.13-0.12 PL). From this study it is concluded that almost all lipid-fractions of freshwater fishes can be predicted easily from total lipid content of the tissue.

Influence of nitrogen salts on growth and physiological responses of Rhizophora apiculata Blume in non-aerated water culture

Influence of two different forms of nitrogen on growth and physiological aspects of water-cultured seedlings of Rhizophora apiculata was studied. Of the two forms of nitrogen supplied to the growth medium, ammonium nitrogen was better than nitrate nitrogen by exhibiting increased dry matter production, shoot length, leaf area and also enhanced the contents of carotenoids, chlorophylls and their presence in photosystems and light harvesting protein complex.

Nitrogen-fixing aerobic bacteria and their nitrogenase activity in north Mumbai coastal waters

The effect of the physicochemical parameters of water and soil on the distribution of nitrogen-fixing bacteria and their nitrogen-fixing capacity was studied. Four species of nitrogen-fixing bacteria, e. g. Azotobacter chroococcum, A. vinelandii, A. beijerinckii and A. armeniacus, were recorded from water and soil samples of Mumbai coast. A higher number of bacterial populations were observed in sediment than in water samples. A positive correlation was observed between the dissolved organic matter and nitrogen fixing bacterial populations of water as well as between available phosphorus and the nitrogen-fixing bacteria of sediment. The nitrogen-fixing capacity of A. chroococcum was found to be 1.076 nmol C sub(2) H sub(4)/l/d and that of A. vinelandii was 0.965 nmol C sub(2) H sub(4)/l/d. Station 1 showed higher level of nitrogenase activity in comparison to other four stations.

The free alpha amino acid nitrogen contents of some common food fishes of Kakinada region, Andhra Pradesh

The moisture and free alpha amino nitrogen contents of some important food fishes and shell fishes of Kakinada region have been studied. Crustaceans and molluscs contain free alpha amino acids in quantities several times higher than all other aquatic animals examined in this study. Their probable role in the physiological activities of these animals has been discussed.

Carbonyls from some commercially important fishes and shell fishes of tropical waters

Results of a preliminary study of the hexane extractable carbonyls from three fish and one shell fish are reported. Volatile carbonyls that can be isolated from oil sardine by distillation at different temperatures in air or nitrogen atmosphere were also studied. Carbonyls were converted to their 2:4 dinitro phenyl hydrozones. These were fractionated by column chromatography and the different fractions were analysed by capillary gas chromatography. The data show wide variations in the content of different carbonyls in different fishes. Carbonyls in distillates from muscle water homogenates of oil sardine showed some differences from the carbonyls extracted with hexane from the same fish. More data is necessary to correlate the carbonyls with the characteristic flavour of each fish.

Advantages of liquid nitrogen freezing of Penaeus monodon over conventional plate freezing

Liquid nitrogen frozen products are biochemically and organoleptically superior to conventional plate frozen products but beneficial effect of liquid nitrogen freezing over conventional plate freezing can exist only up to 59 days at a commercial storage temperature of -18°C.

Nitrogen fixing bacteria, Azotobacter sp. in aquatic sediment

Non-symbiotic, free living, nitrogen fixing bacteria, Azotobacter sp. was estimated in sediments of estuarine, marine, backwater and mangrove environments of Portonovo. Number of colony forming units (CFU) of Azotobacter sp. was less (5 to 27 cells/g of dry sediment). CFU of total heterotrophic bacteria (THB), actinomycetes and fungi were between 4.1x10 super(6) and 4.5x10 super (7), 0.8x10 super(5) and 4.9x10 super(5), 1.1x10 super(5) and 3.8x10 super(5)/g respectively. Mangrove sediments contained more CFU of the above microbial groups.

Effect of liquid nitrogen freezing and subsequent storage on survival of Staphylococcus aureus and Streptococcus pyogenes in treated prawn meat

Prawn meat treated with Streptococcus pyogenes B-49-2 culture and Staphylococcus aureus ATCC-12598 culture were frozen in conventional plate freezer at -40°C and by spray type liquid nitrogen freezer. The frozen products were stored at -18°C. Streptococcus pyogenes B-49-2 showed low sensitivity to cold injury during freezing and frozen storage. Staphylococcus aureus ATCC-12598 survived during the entire storage period of 240 days. Total bacterial count of untreated prawn meat was found to be always lesser in liquid nitrogen frozen products than that in plate frozen products.

Influences of dietary lipid and phosphorus levels on retention and excretion of phosphorus and nitrogen in fingerlings red sea bream, Pagrus major

A laboratory based 2x3 factorial experiment was conducted for 12 weeks to investigate the influences of dietary lipid and phosphorus (P) levels on retention and excretion of phosphorus and nitrogen (N) in fingerling red sea bream. Two levels of lipid (210 and 260 g/kg) and three levels of phosphorus (17, 14 and 12 g/kgˉ¹) in the dry diets were tested. Duplicate groups of 25 red sea bream (average weight 3.74±0.07 g) per 60L glass tank were fed experimental diets three times a day near to satiation level at 22 to 28°C water temperature. A reduction in dietary fish meal from 500 to 300 g/kg dry diet, corresponding to a supplementation in both dietary lipid and P resulted in significant increase in both P and N retention which resulted in the reduction of their excretion by red sea bream. The overall results of the present study demonstrated that both lipid and phosphorus supplementation are necessary for developing less-polluting feed which in turn, reduce fish meal level in the diet of fingerling red sea bream. Further studies in this regard with different size and age groups of red sea bream are warranted.