Culture of green algae Chlorella ellipsoidea in inexpensive media

The culture of the of green alga Chlorella ellipsoidea was conducted under natural conditions at the same place simultaneously in five different media, viz., medium-I (inorganic medium), medium-II (powdered whole-pulse medium), medium-III (medium of pulse bran), medium-IV (mixed medium = 50% inorganic medium + 50% whole-pulse powder medium), medium-V (mixed medium = 50% inorganic medium + 50% pulse bran medium). The culture was done in 500 ml conical flask. Growth rates of C. ellipsoidea in five different media were different and reached maximum cell densities of 0.63 x 10^6 cells per ml in 8 days in medium-I, 4.02 x 10^6 cells per ml in 10 days in medium-II, 3.62 x 10^6 cells per ml in 9 days in medium-III, 4.38 x 10^6 cells per ml in 11 days in medium-IV and 4.36 x 10^6 cells per ml in 11 days in medium-V. The range of air temperature was 20 to 33°C and that of culture media was 24 to 32°C and light intensity was 2000 to 7000 lux during the culture period. The inexpensive culture media were found to be significantly useful for algal culture.

Management of euglenophytes in aquaculture ponds for improvement of fish production

Four experiments each with three replications were conducted in 12 experimental ponds to control the euglenophytes bloom viz. treatment 1 (T1, covering of one third of the water surface by duckweed (Lemna minor); treatment 2 (T2), application of 123.5 kg lime/ha/month; treatment 3 (T3), use of both duckweed as in T1 and lime as in T2; treatment 4 (T4) was considered as control where neither duckweed nor lime was applied. Fishes comprising of rohu (Labeo rohita), catla (Catla catla), mrigal ( Cirrhinus cirrhosus), silver carp (Hypophthalmichthys molitrix) and silver barb (Barbonymus gonionotus) were stocked at the rate of 1080 fishes/ha with the species ratio of 8:4:6:9:13, respectively. The lowest cell density of euglenophytes was found in the ponds of T3 followed by T2, and T1. In the ponds of T3, euglenophytes bloom did not occur possibly due to alkaline pH, shade and nutrient absorption by duckweed. Thin bloom was observed in the ponds of T1 where pH was neutral or slightly alkaline. The grazing on euglenophytes by the silver carp and silver barb also had some contribution in controlling the bloom. Growth of fishes was comparatively higher in the ponds of T3 and T1, which might be due to better water quality and availability of adequate food while the lower fish growth as recorded from the ponds of T4 might be due to euglenophytes bloom. Thick bloom inhibited light penetration which hampered photosynthesis and growth of other phytoplankton that are the preferred food of planktivorous fishes. Mortality of fishes in ponds having euglenophytes bloom was possibly due to formation of anoxic situation in the early morning or due to the combined effect of anoxic situation and toxic metabolites secretion by the euglenophytes.

Preliminary study on the rearing of Chilka prawn ('Kantala') Penaeus indicus H. Milne-Edwards in the brackish water ponds at Keshpur (Orissa)

Results of experimental rearing of Penaeus indicus without supplemental feeding in unfertilized brackish water ponds in fore-shore of Chilka lake are presented in this paper. Higher rate of survival was recorded where advanced juveniles were stocked and lower rate of survival was recorded where early juveniles were stocked. The average growth rate recorded from the rearing experiments was higher than the average growth rate of Penaeus indicus recorded from the adjoining Chilka Lake which indicates the promising future of prawn farming in and around the lake. Analysis of gut contents revealed that Penaeus indicus could efficiently utilise algal and higher plant matter present in the ponds. No noticeable differences in daily growth rates were noticed between early and advanced juveniles in rearing experiments.

Methods of harvesting and preservation of the diatom Chaetoceros calcitrans

Diatom culture and larval feeding experiments were conducted to test the viability and acceptability of preserved algal concentrates. C. calcitrans is characterised by the presence of setae which keep them suspended in cultures and make autoflocculation very difficult. Flocculation was induced by the addition of a floc-forming chemical. Using the optimum conditions, it was possible to harvest the algae within 1-h settling time and with about 84% recovery. The viability of frozen Chaetoceros was determined by actual cell reproduction. Preliminary feeding experiments showed that Chaetoceros can be successfully used as a substitute for fresh diatoms as feed for Penaeus monodon larvae. Simple freezing techniques, with or without the use of protectants has been found convenient for preserving algal concentrates in small volumes for both feeding and culture purposes.

Preserved algae as food for Penaeus monodon larvae

Uni-algal cultures of C. calcitrans, S. costatum, T. chui and Isochrysis sp. obtained from the laboratory were harvested. The harvest, preserved by either freezing or sun-drying, was fed to the larvae of P. monodon . Among the test algal species, Chaetoceros and Tetraselmis were used in larval feeding trials with frozen food while Chaetoceros, Tetraselmis and Isochrysis species were utilized in feeding experiments with sun-dried algae. Their relative effects on larval survival and development were assessed. Results showed that, except the alum-flocculated cells, both frozen Chaetoceros and Tetraselmis can support survival at the zoea stage. Best survival of 68% was atained with dried Chaetoceros followed by Tetraselmis at 44%. Dried Isochrysis did not perform as well, a significantly low survival of only 25% was obtained.

Viability of frozen algae used as food for larval penaeids

Freezing with added chemicals as flocculants and protectants was assessed as a means of preserving stock cultures of 4 algal species used for larval penaeid food Chaetoceros calcitrans, Skeletonema costatum, Tetraselmis chuii and Isochrysis galbana . The maximum storage effectability of the preservation techniques for each species was also determined.

Viability of frozen algae used as food for larval penaeids

Freezing with added chemicals as flocculants and protectants was assessed as a means of preserving stock cultures of 4 algal species used for larval penaeid food Chaetoceros calcitrans, Skeletonema costatum, Tetraselmis chuii and Isochrysis galbana. The maximum storage effectability of the preservation techniques for each species was also determined.

Status of the biodiversity of St. Martin's Island, Bay of Bengal, Bangladesh

A total of 234 species of fish have been recorded from the St Martin Island. Of which, 98 species are coral associated. The total number of recorded mollusc and crab species stands at 187 and 7 species respectively. A total of 66 coral species were recorded, of which 19 are fossil corals, 36 living corals and the rest are under 6 families of subclass Octocorallia (soft corals). A total of 14 species of algae have been recorded from the St. Martin's Island. There is an estimated amount of 1500 MT red sea weed biomass available around the St. Martin's Island. The island contains some of the most unique, benthic community associations in Bangladesh, not found anywhere else in the South Asian region. The unique marine communities have very high scientific value for research and monitoring and there are only a few examples worldwide, where coral-algal communities dominate rocky reefs. The economy of the island is based on fishing. It is estimated that, about 1650 MT of fish are caught annually. Over-exploitation of renewable marine and coastal resources (e.g., rocky reef fisheries, coral and shell extraction; removal of coastal vegetation from inter-tidal and sub-tidal habitats) is a major threat to this ecosystem. Destructive fishing practices, mainly the use of rock-weighted gill nets over the inshore boulder reefs is of prime aggravates. Proper implementation of the rules and regulation for Ecologically Critical Areas (ECA's), alternative livelihood for the local people and further research should be immediately taken for sustainable utilization and to save the rich biodiversity of the only coral island in Bangladesh.

Survey of plankton blooms and red tides and their interaction with environmental factors through monitoring satellite images

One of the most important marine ecological phenomena is red tide which is created by increasing of phytoplankton population, influenced by different factors such as climate condition changes, utrification hydrological factors and can leave sever and undesired ecological and economical effects behind itself in the case of durability. Coast line of Hormozgan is about 900km from east to west, within the range of geographical coordinates of 56 16 23.8, 26 58 8.8 to 54 34 5.33 and 26 34 32 eastern longitude and northern latitude, seven sampling stations were considered and sampled for a period of one year from October 2008 to October 2009. after the analysis of Satellite images, monthly, during the best time. In several stages, samplings were performed. In each station, three samples were collected for identification and determination of Bloom- creating species abundance. Cochlodinium polykrikoides was the species responsible for the discoloration which occurred at October 2008 in Hormozgan marine water. Environmental parameters such as sea surface temperature, pH, salinity, Dissolved Oxygen concentration, Total Dissolved Solids (T.D.S.), conductivity, nitrate, nitrite and phosphate and also chlorophyll a were measured and calculated. Kruscal Wallis test was used to compare the densities between different months, seasons and the studied stations. Mann-whitney test from Nonparametric Tests was used for couple comparison. Pearson correlation coefficient was used to determine the relationship between physical and chemical data set and the abundance of Cochlodinium polykrikoides. Multivariate Regression and analysis of variance (ANOVA) also were used to obtain the models and equations of red tide occurrence relationship, environmental parameters and nutrient data. The highest density was 26 million cells per liter in Qeshm station. A meaningful difference was observed between sampling months and seasons but there was no between sampling stations which indicates that in favorable conditions, the occurrence of this phenomenon by the studied species is probable. Regarding to β coefficients of nitrate, temperature, phosphate, Total Dissolvable Solutions (T.D.S) and pH these parameters are effective on the abundance of this species and red tide occurrence. Increase in these factors can represent the effects and outcomes of human activities and increase in marine pollution.

Assessment of pelagic primary and secondary production in Sefidrud River estuary

Sefid-Rood River Estuary (SRE) is the most important riverine ecosystem in the south Caspian Sea along the Iranian coast lines. The aim of this study was to examine spatial and temporal variability in Phytoplankton and Zooplankton abundance and diversity in SRE. Variability of Chlorophyll a and inorganic nutrient concentration were determined during a year (November 2004– October 2005) in five sampling stations. Primary and secondry production were determined during a year. Total chlorophyll a concentration during the investigation ranged between zero to 22.8 μgl-1 and the highest levels were consistently recorded during summer and the lowest during winter with a annual mean concentration 4.48 μgl-1. Nutrient concentration was seasonally related to river flow with annual mean concentration: NO2 0.05±0.2 mgl-1, NO3 1.13±0.57 mgl-1, NH4 0.51±0.66 mgl-1, total phosphate 0.13±0.1mgl-1 and SiO2 5.68±1.91 mgl-1. Bacillariophytes, Cyanophytes, Chlorophytes, Pyrophytes and Euglenophytes were the dominant phytoplankton groups in this shallow and turbid estuary. The diversity and abundance of phytoplankton had a seasonal pattern while Diatomas and Chrysophytes were dominant throughout the year but Cyanophytes observed only during the summer. Zooplankton community structure was dominated by copepods which 68% of the total zooplankton. In the winter and summer seasons two increased in the number of zooplankton community and usually toward the sea had occurred. Zooplankton also showed a significant spatial and temporal variation. The high turbidity and temperature prime characteristics of SRE seem to be determining factors acting directly on phytoplankton and zooplankton temporal variability and nutrient fluctuations. Everywhere in this estuary nutrients appeared to be in excess of algal requirement and did not influence a phytoplankton and zooplankton composition. Also there was a positive correlation between chlorophyll a and temperature and a negative one with DIN and TP. Primary production determined in this estuary by dark and light butter method and G.P.P. 38.27±34.12 mgcm-2h-1 and N,PP 201.6±289.9 mgcm-2d-1. secondry production determined 15/128 mgc/m3/year. Everywhere in this estuary nutrients appeared to be in excess to algal requirement and did not influence in Chl. a and primary production. The most important factor influence on Chl. a was water temperature.

Effects of cage fish culture on water quality and selected biological communities in northern Lake Victoria, Uganda

Growing of fish in cages is currently practiced in Uganda and was first introduced in northern Lake Victoria in 2010. An environment monitoring study was undertaken at Source of the Nile, a private cage fish farm, in Napoleon gulf, northern Lake Victoria. In-situ measurements of key environmental (temperature, dissolved oxygen, pH and conductivity) and biological (algae, zooplankton, macro-benthos) variables were made at three transects: Transect 1- the site with fish cages (WC); transect 2- upstream of the fish cages (USC-control) and Transect 3- downstream of the cages (DSC). Upstream and Downstream sites were located approximately 1.0 km from the fish cages. Environment parameters varied spatially and temporally but were generally within safe ranges for freshwater habitats. Higher concentrations of SRP (0.015-0.112 Mg/L) occurred at USC during February, September and at DSC in November; NO2-N (0.217- 0.042 mg/L) at USC and DSC in February and November; NH4-N (0.0054- 0.065 Mg/L) at WC and DSC in February, May and November. Algal bio-volumes were significantly higher at WC (F (2,780)=4.619; P=0.010). Zooplankton species numbers were consistently lower at WC with a significant difference compared to the control site (P=0.032). Macro-benthos abundance was consistently higher at the site with cages where mollusks and low-oxygen and pollution-tolerant chironomids were the dominant group. Higher algal biomass, concentration of low-oxygen/pollution-tolerant macro-benthos and depressed zooplankton diversity at WC suggested impacts from the fish cages on aquatic biota.

Physical and chemical characteristics of major aquatic ecosystems in the Victoria and Kyoga lake basins

The physical-chemical characteristics of any aquatic ecosystem include pH, conductivity, and temperature, water transparency, nutrient and the chlorophyll-a levels. Physical and chemical factors of any ecosystem determine the type and quality of flora present in it and these forms the basis on which the system operates. The elements required in largest amounts for plant productions are carbon, phosphorus, nitrogen, and silicon, which is important for diatoms as a major component of the cell wall. Nutrients may limit algal productivity in the tropics despite the high temperature there allowing rapid nutrient recycling. Nutrients most likely to be limiting African lakes are nitrogen (Talling & Talling 1965; Moss 1969; Lehman & Branstrator 1993, 1994) and phosphorus (Melack.et al l982; Kalff 1983) while silicon may limit diatom growth (Hecky & Kilham 1988). The objective of the study is to investigate the impact of physical-chemical characteristics on the distribution and abundance of organisms in the major aquatic ecosystems.

The diversity and abundance of zooplankton in the Lake Victoria and Kyoga basins and their relationship to fish production

Biological diversity of an ecosystem is considered a reliable measure of the state of health of the ecosystem. In Uganda's large lakes, the Victoria and Kyoga, the past three decades have been characterized by profound changes in fish species composition following the introduction of the piscivorous Nile perch (Oguto-Ohwayo 1990). Over 300 haplochromine cichlid species comprising a wide range of trophic groups were lost along with a host of non-cichlid fishes which occupied virtually all available ecological niches and in the lakes (Witte 1992). A second major ecological event has been the gradual nutrient enrichment of the water bodies (eutrophication) from diffuse and point sources, while at the same time pollutants have also gained entrance into the water systems in pace with indusfrial development and human population increases in the lake basins. Eutrophication and pollution have drastically altered the physical and-chemical character of the water medium in which different fauna and flora thrive. In Lake Victoria these alterations have resulted in changes of algal species composition from pristine community dominated by chlorophytes and diatoms (Melosira etc) to one composed largely of blue-green algae or Cyanobacteria (Microcystis, Anabaena, Planktolyngbya etc) (Mugidde 1993, Hecky 1993).

Technical report on the environmental monitoring of the cage area at the Source of the Nile (SON) Fish Farm for quarter 2: April-June 2015

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm have a collaborative arrangement with NaFIRRI to undertake quarterly environment monitoring of the cage site as is mandatory under the NEMA conditions. The monitoring surveys cover selected physical-chemical factors i.e. water column depth, water transparency, water column temperature, dissolved oxygen, pH and conductivity; nutrient status, algal and invertebrate communities (micro-invertebrates/zooplankton and macroinvertebrates/ macro-benthos) as well as fish community. The second quarter survey for the calendar year 2015, which is the subject of this report, was undertaken in June 2015. Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and aquatic biota.

Effects of inlake application of glyphoste and 2, 4‐D to control shoreline water hyacinth on diversity and abundance of associated macrofauna

The purpose of inlake herbicide trials was to assess on the aquatic environment and resources, of in-lake of weeder 64 (2,4-0 amine) and Rodio (Glyphosate) water hyacinth the effects application to control water hyacinth. The experiments reported here specifically studied the effects of the herbicides on the diversity and abundance of aquatic macrofauna associated with the water weed. Results from this and similar experiments which assessed herbicide efficacy on water hyacinth; dissipation in water, impact on water quality, algal biomass and on diversity and abundance of zooplankton and macrofauna were all to be evaluated as input into the environmental impact assessment exercise required to facilitate decisions on the use of herbicides to control water hyacinth in Uganda.