2 resultados para Canadian Bank of Commerce
em CaltechTHESIS
Resumo:
Pre-mRNA splicing requires interaction of cis- acting intron sequences with trans -acting factors: proteins and small nuclear ribonucleoproteins (snRNPs). The assembly of these factors into a large complex, the spliceosome, is essential for the subsequent two step splicing reaction. First, the 5' splice site is cleaved and free exon 1 and a lariat intermediate (intron- exon2) form. In the second reaction the 3' splice site is cleaved the exons ligated and lariat intron released. A combination of genetic and biochemical techniques have been used here to study pre-mRNA splicing in yeast.
Yeast introns have three highly conserved elements. We made point mutations within these elements and found that most of them affect splicing efficiency in vivo and in vitro, usually by inhibiting spliceosome assembly.
To study trans -acting splicing factors we generated and screened a bank of temperature- sensitive (ts) mutants. Eleven new complementation groups (prp17 to prp27) were isolated. The four phenotypic classes obtained affect different steps in splicing and accumulate either: 1) pre-mRNA, 2) lariat intermediate, 3) excised intron or 4) both pre-mRNA and intron. The latter three classes represent novel phenotypes. The excised intron observed in one mutant: prp26 is stabilized due to protection in a snRNP containing particle. Extracts from another mutant: prpl8 are heat labile and accumulate lariat intermediate and exon 1. This is especially interesting as it allows analysis of the second splicing reaction. In vitro complementation of inactivated prp18 extracts does not require intact snRNPs. These studies have also shown the mutation to be in a previously unknown splicing protein. A specific requirement for A TP is also observed for the second step of splicing. The PRP 18 gene has been cloned and its polyadenylated transcript identified.
Resumo:
I. Foehn winds of southern California.
An investigation of the hot, dry and dust laden winds
occurring in the late fall and early winter in the Los Angeles
Basin and attributed in the past to the influences of the desert
regions to the north revealed that these currents were of a
foehn nature. Their properties were found to be entirely due
to dynamical heating produced in the descent from the high level
areas in the interior to the lower Los Angeles Basin. Any dust
associated with the phenomenon was found to be acquired from the
Los Angeles area rather than transported from the desert. It was
found that the frequency of occurrence of a mild type foehn of this
nature during this season was sufficient to warrant its classification
as a winter monsoon. This results from the topography of
the Los Angeles region which allows an easy entrance to the air
from the interior by virtue of the low level mountain passes north
of the area. This monsoon provides the mild winter climate of
southern California since temperatures associated with the foehn
currents are far higher than those experienced when maritime air
from the adjacent Pacific Ocean occupies the region.
II. Foehn wind cyclo-genesis.
Intense anticyclones frequently build up over the high level
regions of the Great Basin and Columbia Plateau which lie between
the Sierra Nevada and Cascade Mountains to the west and the Rocky
Mountains to the east. The outflow from these anticyclones produce
extensive foehns east of the Rockies in the comparatively low
level areas of the middle west and the Canadian provinces of
Alberta and Saskatchewan. Normally at this season of the year very
cold polar continental air masses are present over this territory
and with the occurrence of these foehns marked discontinuity surfaces
arise between the warm foehn current, which is obliged to slide over
a colder mass, and the Pc air to the east. Cyclones are
easily produced from this phenomenon and take the form of unstable
waves which propagate along the discontinuity surface between the
two dissimilar masses. A continual series of such cyclones was
found to occur as long as the Great Basin anticyclone is maintained
with undiminished intensity.
III. Weather conditions associated with the Akron disaster.
This situation illustrates the speedy development and
propagation of young disturbances in the eastern United States
during the spring of the year under the influence of the conditionally
unstable tropical maritime air masses which characterise the
region. It also furnishes an excellent example of the superiority
of air mass and frontal methods of weather prediction for aircraft
operation over the older methods based upon pressure distribution.
IV. The Los Angeles storm of December 30, 1933 to January 1, 1934.
This discussion points out some of the fundamental interactions
occurring between air masses of the North Pacific Ocean in connection
with Pacific Coast storms and the value of topographic and
aerological considerations in predicting them. Estimates of rainfall
intensity and duration from analyses of this type may be made and
would prove very valuable in the Los Angeles area in connection with
flood control problems.