Summer deficit irrigation strategies in hedgerow olive orchard cv. ‘Arbequina’: Effect on fruit characteristics and yield.

Maximum production in hedgerow olive orchards is likely not achieved with maximum evapotranspiration over the long-term. Thus, regulated deficit irrigation (RDI) should be considered as a management option. Four irrigation treatments were evaluated during the summer when olive is most drought resistant. Control (CON) was irrigated to maintain the rootzone close to field capacity. Severe water deficit was applied by irrigating 30% CON from end of fruit drop to end July (DI-J) and from end July until beginning of oil synthesis (DI-A). Less severe water deficit was applied during July and August (DI-JA) by irrigating 50% CON. Flowering, fruiting, abscission, fruit development, fresh and dry weight of fruits, and oil production were evaluated. There were not significant differences in number of buds initiated, number of fruits per inflorescence and fruit drop. Oil production was significantly different between irrigation treatments in all experimental years. CON produced more oil and fruit with higher oil% than DI-A and DI-JA. Oil production of DI-J was not significantly reduced compared to CON and oil% was greater. DI-J was the most effective RDI strategy; with 16% less applied water relative to CON average loss in oil production of 8% was not significantly different to CON. While DI-JA saved most water (27%), oil production was reduced by 15%. Greatest loss in oil production (21%) was observed in DI-A with water saving of 22%.

Human midbrain precursors activate the expected developmental genetic program and differentiate long-term to functional A9 dopamine neurons in vitro. Enhancement by Bcl-XL

Understanding the molecular programs of the generation of human dopaminergic neurons (DAn) from their ventral mesencephalic (VM) precursors is of key importance for basic studies, progress in cell therapy, drug screening and pharmacology in the context of Parkinson's disease. The nature of human DAn precursors in vitro is poorly understood, their properties unstable, and their availability highly limited. Here we present positive evidence that human VM precursors retaining their genuine properties and long-term capacity to generate A9 type Substantia nigra human DAn (hVM1 model cell line) can be propagated in culture. During a one month differentiation, these cells activate all key genes needed to progress from pro-neural and prodopaminergic precursors to mature and functional DAn. For the first time, we demonstrate that gene cascades are correctly activated during differentiation, resulting in the generation of mature DAn. These DAn have morphological and functional properties undistinguishable from those generated by VM primary neuronal cultures. In addition, we have found that the forced expression of Bcl-XL induces an increase in the expression of key developmental genes (MSX1, NGN2), maintenance of PITX3 expression temporal profile, and also enhances genes involved in DAn long-term function, maintenance and survival (EN1, LMX1B, NURR1 and PITX3). As a result, Bcl-XL anticipates and enhances DAn generation.

Developmental Expression of Kv Potassium Channels at the Axon Initial Segment of Cultured Hippocampal Neurons

Axonal outgrowth and the formation of the axon initial segment (AIS) are early events in the acquisition of neuronal polarity. The AIS is characterized by a high concentration of voltage-dependent sodium and potassium channels. However, the specific ion channel subunits present and their precise localization in this axonal subdomain vary both during development and among the types of neurons, probably determining their firing characteristics in response to stimulation. Here, we characterize the developmental expression of different subfamilies of voltage-gated potassium channels in the AISs of cultured mouse hippocampal neurons, including subunits Kv1.2, Kv2.2 and Kv7.2. In contrast to the early appearance of voltage-gated sodium channels and the Kv7.2 subunit at the AIS, Kv1.2 and Kv2.2 subunits were tethered at the AIS only after 10 days in vitro. Interestingly, we observed different patterns of Kv1.2 and Kv2.2 subunit expression, with each confined to distinct neuronal populations. The accumulation of Kv1.2 and Kv2.2 subunits at the AIS was dependent on ankyrin G tethering, it was not affected by disruption of the actin cytoskeleton and it was resistant to detergent extraction, as described previously for other AIS proteins. This distribution of potassium channels in the AIS further emphasizes the heterogeneity of this structure in different neuronal populations, as proposed previously, and suggests corresponding differences in action potential regulation.

Summer deficit irrigation in a hedgerow olive orchard cv. Arbequina: relationship between soil and tree water status, and growth and yield components

El potencial hídrico del tronco es una herramiento útil para el manejo del riego. Los umbrales de riego deben establecerse para cada periodo fisiológico. En este experimento, realizado en Arbequina en seto, se estudio la relacion entre los potenciales hídricos y la produccion de aceite. Cuando los potenciales hidricos son inferiores a -1.3 MPa el crecimiento vegetativo se reduce mas del 50%. En cuanto a la produccion, se observó que regando en Julio cuando se alcanzan potenciales cercanos a -2.9 MPa se puede ahorrar agua sin afectar a la produccion. Sin embargo en Agosto el potencial debe mantenerse por encima de -2 MPa para que no se resienta la producción.

Summer deficit-irrigation strategies in a hedgerow olive cv. Arbequina orchard: Effect on oil quality

En este ensayo se evaluó el efecto del déficit hídrico en el verano en la calidad del aceite de la variedad Arbequina. Para ello se establecieron 3 tratamientos de riego deficitario y un Control en un olivar en seto. El deficit severo aplicado en Julio provocó un incremento en polifenoles comparado con los otros tratamientos.

Summer deficit-irrigation strategies in a hedgerow olive cv. Arbequina orchard: Effect on oil quality

En un olivar en seto de la variedad Arbequina, situado en la Puebla de Montalbán (Toledo), se aplicaron diferentes estrategias de riego deficitario durante el verano. Se evaluó el efecto del riego sobre la calidad del aceite. Los aceite procedentes del tratamiento de deficit severo de julio presentarion una mayor estabilidad oxidativa, lo que coincide con un mayor contenido en polifenoles

Iron distribution through the developmental stages of Medicago truncatula nodules.

Paramount to symbiotic nitrogen fixation (SNF) is the synthesis of a number of metalloenzymes that use iron as a critical component of their catalytical core. Since this process is carried out by endosymbiotic rhizobia living in legume root nodules, the mechanisms involved in iron delivery to the rhizobia-containing cells are critical for SNF. In order to gain insight into iron transport to the nodule, we have used synchrotron-based X-ray fluorescence to determine the spatio-temporal distribution of this metal in nodules of the legume Medicago truncatula with hitherto unattained sensitivity and resolution. The data support a model in which iron is released from the vasculature into the apoplast of the infection/differentiation zone of the nodule (zone II). The infected cell subsequently takes up this apoplastic iron and delivers it to the symbiosome and the secretory system to synthesize ferroproteins. Upon senescence, iron is relocated to the vasculature to be reused by the shoot. These observations highlight the important role of yet to be discovered metal transporters in iron compartmentalization in the nodule and in the recovery of an essential and scarce nutrient for flowering and seed production.

Quality attributes of pistachio nuts as affected by rootstocks and deficit irrigation

BACKGROUND: In this work, the influence of two regulated deficit irrigation (RDI) treatments and three different rootstocks on the quality of pistachios was evaluated by analyzing different parameters: morphological analysis, physicochemical analysis and sensory analysis. RESULTS: The results obtained in terms of the choice of rootstock revealed that Pistacia atlantica had increased production yields, nut weight, mineral content, higher intensities of characteristic sensory attributes and a higher degree of consumer satisfaction, than the other rootstocks studied. Moreover, the results established that the application of RDI on pistachio cultivation had no significant influence on production yield, weight, size, colour, water activity or mineral composition. Furthermore, T1 treatment (stem water potential?deficit irrigation (T1) contributes to an increase in overall product quality. Furthermore, Pistacia atlantica rootstock provided better yield and quality than the other rootstocks studied. © 2014 Society of Chemical Industry

Effect of the season on the free phytoprostanes content in Cornicabra extra virgin olive oil from deficit irrigated olive trees

BACKGROUND: The effect of regulated deficit irrigation (RDI) on the phytoprostane (PhytoP) content in extra virgin olive (Olea europaea L., cv. Cornicabra) oil (EVOO) was studied. During the 2012 and 2013 seasons, T0 plants were irrigated at 100% ETc, while T1 and T2 plants were irrigated avoiding water deficit during phases I and III of fruit growth and saving water during the non-critical phenological period of pit hardening (phase II), developing amore severewater deficit in T2 plants. In 2013, a fourth treatment (T3) was also performed, which was similar to T2 except that water saving was from the beginning of phase II to 15 days after the end of phase II. RESULTS: 9-F1t-PhytoP, 9-epi-9-F1t-PhytoP, 9-epi-9-D1t-PhytoP, 9-D1t-PhytoP, 16-B1-PhytoP and 9-L1-PhytoP were present in Cornicabra EVOO, and their contents increased in the EVOO fromRDI plants. CONCLUSION: Deficit irrigation during pit hardening or for a further period of 2 weeks thereafter to increase irrigation water saving is clearly critical for EVOO composition because of the enhancement of free PhytoPs, which have potential beneficial effects on human health. The response of individual free PhytoPs to changes in plant water status was not as perceptible as expected, preventing their use as biomarkers of water stress.