Identification of Ultraviolet radiation induced Gallic acid and Caffeic acid formation in Palm juice (Borassus flabellifer) by HPLC & Mass Spectra Technique.

Palm juice, a common-cheap-antioxidants rich natural plant juice has been investigated for optimizing the effect of UV-radiation on the antioxidant activity using a DPPH free radical scavenging activity method. In this study separate set of samples of raw palm juice has been treated with 365 and 254 nm UV-lights (UVL) respectively for different exposure time. When exposed for 15 min with 365 nm UVL induces concentration factor of caffeic acid, whereas, 254 nm UVL induces gallic acid accumulation, but overall antioxidant activity was higher for 365 nm UVradiation. Caffeic acid and other polyphenol compounds are increased by 5.5 ± 0.5 % than normal palm juice, observed after irradiation with 365 nm UVL. Even after the exposure of UV irradiation for 15 min, did not affect on peptide bond modification of protein molecules present in palm juice, therefore a green effect of UVL is explored for the effective increase of antioxidant activity.

Estudio de la estabilidad genética asociada a los procesos de crioconservación de ápices de menta

La crioconservación se ha descrito como una técnica de conservación ex situ a largo plazo que ha sido aplicada con éxito a numerosas especies, y resulta especialmente importante en aquellas con propagación vegetativa, infértiles o amenazadas, en las que sistemas de conservación ex situ más sencillos, como los bancos de semillas, no son posibles. También presenta ventajas frente a la conservación in vitro, ya que logra disminuir o eliminar problemas como la excesiva manipulación del material, evitando los subcultivos periódicos y disminuyendo así el riesgo de contaminaciones y de aparición de variación somaclonal. Sin embargo, someter al material vegetal a los procedimientos que implica la crioconservación provoca distintos estreses. Entre ellos, el estrés oxidativo puede potencialmente producir daños en membranas, proteínas, carbohidratos y en el ADN. En este trabajo se han evaluado diversos sistemas de crioconservación en ápices de Mentha × piperita L., híbrido estéril entre Mentha aquatica L. y Mentha spicata L. Se han utilizado ápices de dos genotipos (‘MEN 186’y ‘MEN 198’) en los cuales se compararon dos técnicas de crioconservación, encapsulación-deshidratación y vitrificación-droplet. El análisis de la supervivencia y capacidad de regeneración del material sometido a los tratamientos de crioconservación, junto con el análisis de la estabilidad genética de dicho material mediante marcadores moleculares (RAPD y AFLP) han permitido comparar los distintos protocolos y tratamientos establecidos. El estudio sobre el tipo de protocolo empleado reveló una mayor variabilidad genética en la técnica de encapsulación-deshidratación, especialmente en el genotipo ‘MEN 186’, ya que ‘MEN 198’ resultó ser más estable en todos los análisis. La inestabilidad encontrada en esta técnica no fue exclusiva de aquellos explantos crioconservados, sino que los pasos previos a la inmersión en nitrógeno líquido (NL) también provocaron variaciones en el ADN. Según el tipo de muestra analizada se encontraron diferencias en la estabilidad: muestras provenientes de callos presentaron una mayor inestabilidad que aquellas de hojas (brotes). Se utilizaron tres medios para la recuperación de los ápices tras la crioconservación con el uso de diferentes combinaciones de reguladores de crecimiento: “Reed” (0,5 mgL-1 6-bencilaminopurina, BAP), “Senula” (0,5 mgL-1 6-dimetilalilamino-purina, 2-iP + 0,1 mgL-1 ácido α-naftalen-acético, ANA) y “Nudos” (0,5 mgL-1 BAP + 0,1 mgL-1ANA). El medio “Reed” produjo un aumento en la supervivencia y recuperación de los ápices en ambos genotipos y técnicas, y disminuyó la formación de callo. Sin embargo, no tuvo un efecto significativo en la estabilidad genética. El medio “Senula” provocó una mayor estabilidad genética en el genotipo más inestable, ‘MEN 186’. Para reducir el daño oxidativo producido durante la encapsulación-deshidratación, e incrementar la recuperación de los ápices manteniendo su estabilidad genética, se comparó el efecto de añadir sustancias antioxidantes en el precultivo de los ápices (ácido ascórbico, vitamina E y glutatión). No se obtuvo la respuesta esperada y estos tratamientos no presentaron efectos significativos tanto en la estabilidad como en la recuperación. Para entender mejor qué sucede durante todo el proceso de encapsulación-deshidratación, se evaluó cada paso del protocolo por separado y su efecto en la estabilidad y la recuperación. Además, se determinó el estado de oxidación en cada etapa mediante la cuantificación de malondialdehído y la detección de la formación de radicales libres (mediante el ensayo del ácido tiobarbitúrico, y sondas fluorescentes específicas, respectivamente). Se determinó que a partir de los primeros pasos se genera estrés oxidativo, el cual aumenta a medida que se avanza por el protocolo hasta la inmersión en nitrógeno líquido. Esto se ve reflejado en la disminución progresiva tanto de la recuperación como de la estabilidad genética. Con el uso de antioxidantes en el precultivo (ácido ascórbico y vitamina E) no se obtuvo un efecto positivo en el mantenimiento de la estabilidad genética, y tan sólo con el uso de vitamina E se observó una recuperación mayor en uno de los pasos estudiados (después de la desecación). Sin embargo, cuando se utilizó ácido ascórbico durante el precultivo o la deshidratación osmótica se consiguió disminuir de forma significativa la formación de MDA y la acumulación del radical superóxido (O2•-) en la mayoría los pasos analizados, aunque esta reducción no parece tener un efecto directo en la estabilidad genética del material recuperado. ABSTRACT Cryopreservation has been described as an effective technique for the long term of ex situ conservation that has been successfully applied to numerous species, and is of especial relevance for those with vegetative propagation, infertile or endangered, in which simpler systems of ex situ conservation, such as seed banking, are not feasible. It also has advantages over in vitro conservation, as it reduces or eliminates excessive material handling, avoids periodic subcultures and thus limits the risk of contamination and the appearance of somaclonal variation. However, plant material is subjected to different treatments involved in the cryopreservation procedures, which impose several stresses. Among them, oxidative stress can potentially cause damage to membranes, proteins, carbohydrates and DNA. In this work, two cryopreservation techniques have been evaluated in Mentha × piperita L. shoot tips, sterile hybrid between Mentha aquatica L. and Mentha spicata L. Two genotypes ('MEN 186' and 'MEN 198') were used to compare two techniques: encapsulation-dehydration and droplet-vitrification. The analysis of survival and recovery capacity of the material after the cryopreservation treatments, and the analysis of the genetic stability by molecular markers (RAPD and AFLP) have enabled the comparison between protocols and treatments. The study of the two cryopreservation procedures revealed a higher genetic variability in the encapsulation-dehydration technique, especially in genotype 'MEN 186', as 'MEN 198' was more stable in all analyses. The instability generated in this technique was not exclusive of cryopreserved explants, pretreatments prior to immersion in NL also caused DNA variations. The type of sampled plant material revealed also differences in the stability: callus samples showed greater instability than shoots. Three different culture media were used for the recovery of shoot tips after cryopreservation, using different combinations of growth regulators: "Reed" (0.5 mgL-1 6-benzylaminopurine, BAP), "Senula" (0.5 mgL-1 6-dimetilalilamino-purine, 2-iP + 0.1 mgL-1 α-naphthalene acetic acid, ANA) and "Nodes" (0.5 mgL-1 BAP + 0.1 mgL-1 ANA). "Reed" medium increased survival and recovery of shoot tips in both genotypes and techniques and decreased callus formation. However, it didn`t have a significant effect on genetic stability. "Senula" medium caused a higher genetic stability in the most unstable genotype, 'MEN 186'. To reduce oxidative damage during encapsulation-dehydration, and increase shoot tip recovery and maintain genetic stability, the effect of added antioxidants (ascorbic acid, vitamin E and glutathione) in the shoot tip preculture medium was studied. These treatments had no significant effect on both stability and recovery. To better understand the events during the encapsulation-dehydration process, the effect of each step of the protocol on stability and recovery was evaluated separately. Moreover, the oxidation level was determined by quantifying malondialdehyde (MDA) formation and detecting free radical accumulation (using the thiobarbituric acid assay, and specific fluorescent probes, respectively). The oxidative stress was detected from the first steps and increased throughout the protocol until the immersion in liquid nitrogen. This was also reflected in the gradual decline of recovery and genetic stability. The use of antioxidants (ascorbic acid and vitamin E) in the shoot tip preculture medium had no effect in maintaining genetic stability; only vitamin E increased recovery in one of the steps studied (after desiccation). However, when ascorbic acid was used during the preculture or during the osmotic dehydration, a significantly decrease was observed in MDA formation and superoxide radical accumulation in most of the steps analyzed, although this reduction did not seem to have a direct effect on the genetic stability of recovered material.

Generating Scale-free Networks with Adjustable Clustering Coefficient Via Random Walks

This paper presents an algorithm for generating scale-free networks with adjustable clustering coefficient. The algorithm is based on a random walk procedure combined with a triangle generation scheme which takes into account genetic factors; this way, preferential attachment and clustering control are implemented using only local information. Simulations are presented which support the validity of the scheme, characterizing its tuning capabilities.

Novel free-form optical surface design with spiral symmetry

Manufacturing technologies as injection molding or embossing specify their production limits for minimum radii of the vertices or draft angle for demolding, for instance. These restrictions may limit the system optical efficiency or affect the generation of undesired artifacts on the illumination pattern when dealing with optical design. A novel manufacturing concept is presented here, in which the optical surfaces are not obtained from the usual revolution symmetry with respect to a central axis (z axis), but they are calculated as free-form surfaces describing a spiral trajectory around z axis. The main advantage of this new concept lies in the manufacturing process: a molded piece can be easily separated from its mold just by applying a combination of rotational movement around axis z and linear movement along axis z, even for negative draft angles. The general designing procedure will be described in detail

Towards the automatic generation of card games through Grammar-Guided Genetic Programming

We demonstrate generating complete and playable card games using evolutionary algorithms. Card games are represented in a previously devised card game description language, a context-free grammar. The syntax of this language allows us to use grammar-guided genetic programming. Candidate card games are evaluated through a cascading evaluation function, a multi-step process where games with undesired properties are progressively weeded out. Three representa- tive examples of generated games are analysed. We observed that these games are reasonably balanced and have skill ele- ments, they are not yet entertaining for human players. The particular shortcomings of the examples are discussed in re- gard to the generative process to be able to generate quality games

High-frequency signal generation using 1550 nm VCSEL subject to two-frequency optical injection

We experimentally investigate high-frequency microwave signal generation using a 1550 nm single-mode VCSEL subject to two-frequency optical injection. We first consider a situation in which the injected signals come from two similar VCSELs. The polarization of the injected light is parallel to that of the injected VCSEL. We obtain that the VCSEL can be locked to one of the injected signals, but the observed microwave signal is originated by beating at the photodetector. In a second situation we consider injected signals that come from two external cavity tunable lasers with a significant increase of the injected power with respect to the VCSEL-by-VCSEL injection case. The polarization of the injected light is orthogonal to that of the free-running slave VCSEL. We show that in this case it is possible to generate a microwave signal inside the VCSEL cavity. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Depth perceptual video coding for free viewpoint video based on H.264/AVC

A novel scheme for depth sequences compression, based on a perceptual coding algorithm, is proposed. A depth sequence describes the object position in the 3D scene, and is used, in Free Viewpoint Video, for the generation of synthetic video sequences. In perceptual video coding the human visual system characteristics are exploited to improve the compression efficiency. As depth sequences are never shown, the perceptual video coding, assessed over them, is not effective. The proposed algorithm is based on a novel perceptual rate distortion optimization process, assessed over the perceptual distortion of the rendered views generated through the encoded depth sequences. The experimental results show the effectiveness of the proposed method, able to obtain a very considerable improvement of the rendered view perceptual quality.

Numerical Investigation of the Flow Features around Heave Plates Oscillating Close to a Free Surface or Seabed

Performance of heave plates used in offshore structures is strongly influenced by their added mass and damping, which are affected by proximity to a boundary. A previous paper by the authors presented numerical simulations of the flow around a circular solid disk oscillating at varying elevations from seabed [1]. The force calculated was used to evaluate the added mass and damping coefficients for the disk. The simulations suggest that as the structure moves closer to the seabed the added mass and damping coefficients (Ca and Cb) increases continuously. In order to understand the physics behind the added mass and damping trends, when a heave plate is moving near a seabed or closer to the free surface, the flow characteristics around the heave plate are examined numerically in this paper. Flow around oscillating disks is dominated by generation and development of phase-dependent vortical structures, characterized by the KC number and the distance from the seabed or free surface to the heave plate. Numerical calculations presented in this paper have comprised the qualitative analysis of the vortex shedding and the investigation of the links between such vortex shedding and, on one hand the damping coefficient, and on the other hand, pairing mechanisms such as the shedding angle.

La opción radical : viaje a través de Superstudio

Entre 1966 y 1967 un pequeño grupo de jóvenes arquitectos recién titulados en la Facultad de Arquitectura de Florencia escenifican en dos actos programados en las ciudades de Pistoia y Módenala exposición Superarchitettura, con la que sin saberlo inauguran un corto pero intenso periodo de experimentación arquitectónica que convulsionaría Italia durante los 10 años posteriores, dinamitados por multitud de propuestas que darán lugar a la última arquitectura con aspiraciones socio-políticas responsable de un vibrante legado teórico que proclamaba la vuelta a las raíces olvidadas de la profesión y que se mostraba más preocupada por reflexionar sobre sus fundamentos que por perpetuar la práctica de un oficio ahora puesto en crisis. Ambos eventos suponen también el nacimiento simultáneo de Superstudio y Archizoom, dos de los colectivos más influyentes de la arquitectura experimental italiana de estos años, convertidos en protagonistas de un agitado periodo de transición en el que una generación estaba dando paso a la siguiente a través de la sustitución de los principios doctrinarios recibidos de la modernidad por una jugosa incertidumbre desmitificadora ligada a una nueva realidad económica, política y social que marcaría los inicios de la posmodernidad. La experiencia italiana surge aprovechando la efervescencia de un fenómeno que posteriormente algunos críticos etiquetaron como Arquitectura Radical con la intención de unificar el trabajo de un heterogéneo y atomizado grupo de arquitectos fundamentalmente europeos – individualmente comprometidos con el replanteamiento total de la definición y objetivos de la disciplina, al tiempo que participaban en una particular cruzada existencial mediante la que pretendían, si no cambiar el mundo, al menos romper la dinámica estéril de la arquitectura profesional y tecnológica heredada. La presente tesis fomenta la recuperación interesada de este breve momento radical como un ambicioso proyecto de modernización inacabado, pero no solo para analizar las causas de su precipitado final, sino sobre todo para mostrar las razones de su gran éxito internacional, convirtiendo para ello el trabajo de Superstudio, ejemplo paradigmático de la actitud crítica compartida por el resto de operadores contemporáneos, en el catalizador adecuado a partir del cual interpretar y extrapolar su legado con el fin de narrar el cambio trascendental que este episodio supuso para la práctica arquitectónica, alejándola del diseño de edificios y centrándola en la adopción y aplicación de multitud de actividades no tectónicas en un intento por separarse de lo que era percibido como una disciplina corrupta. A lo largo de su corta carrera en común, los seis miembros de Superstudio sustituyeron la construcción convencional por la explotación intensiva de medios alternativos desde el diseño de mobiliario e iluminación y algunos experimentos en torno a la “arquitectura eléctrica”, hasta la expansión mediática de su trabajo a través de revistas, películas, exposiciones y conferencias contribuyendo así a una provocativa destrucción y posterior expansión de los límites dentro de los que hasta ese momento se había mantenido cómodamente instalada la arquitectura. Esta investigación descubre la trascendencia de considerar aquello que ocupó gran parte de la actividad de Superstudio y de sus colegas radicales (muebles, revistas, instalaciones, películas, labor teórica y docente, etc.) como nuevas formas de arquitectura con las que construir un nuevo lenguaje crítico, en un intento por contribuir a renovar y reforzar la relevancia y responsabilidad social de una profesión llamada a expandirse más allá de lo construido para nutrirse e interactuar con todos los ámbitos de nuestro entorno diario. ABSTRACT Between 1966 and 1967 a small group of newly qualified young architects from the Faculty of Architecture of Florence staged in two events planned in the cities of Pistoia and Modena the Superarchitettura exhibition, with which unwittingly inaugurated a short but intense period of architectural experimentation that convulse Italy during the following 10 years, blown up by a multitude of proposals that will result in the latest architecture with socio political aspirations responsible for a vibrant theoretical legacy proclaiming a return to forgotten roots of the profession and that was more concerned with reflect on its fundamentals than perpetuate the practice of a profession now put into crisis. Both events also involve the simultaneous birth of Superstudio and Archizoom, two of the most influential groups of Italian experimental architecture of these years, turned into protagonists of an hectic period of transition in which a generation was giving way to the next through replacing the received doctrinal principles of modernity by a juicy demystifying uncertainty linked to a new reality economic, political and social that would mark the beginning of postmodernism. The Italian experience arises taking advantage of the commotion of a phenomenon that later some critics labeled as Radical Architecture with the intention of unifying the work of a diverse and fragmented group of architects mainly European individually committed to the total rethinking of the definition and objectives of the discipline, while participating in a particular existential crusade with which they sought to, if not change the world, at least break out of the sterile dynamic of professional and technological architecture inherited. This doctoral thesis promotes an interested recovery of in this short radical time as an ambitious modernization project unfinished, but not only to analyze the causes of its precipitous end, but above all to show the reasons for it’s international success, turning the work of Superstudio, a paradigmatic example of the critical attitude shared by the rest of contemporary operators, into the suitable catalyst from which to interpret and extrapolate it’s legacy in order to recount the significant change that this episode marked for architectural practice, moving it away from building and focusing it on the adoption and implementation of many nonLtectonic activities in an attempt to separate from what was perceived as a corrupt discipline. Throughout their short career in common, the six members of Superstudio replaced conventional construction by intensive exploitation of alternative means L from furniture and lighting design and some experiments around the "electric architecture", to the media expansion of their work through magazines, films, exhibitions and lectures thus contributing to a provocative destruction and subsequent expansion of the limits within which architecture had remained ensconced until then. This dissertation finds out the importance of considering what occupied much of the activity of Superstudio and their radical colleagues (furniture, magazines, installations, films, theoretical and educational work, etc.) as new forms of architecture that build a new critical language, in an attempt to help renew and reinforce the relevance and social responsibility of a profession call to expand beyond the built to nourish and interact with all areas of our everyday environment.

Cost-free feed-in tariffs for renewable energy deployment in Spain

Feed-in-tariff (FIT) schemes have been widely employed to promote renewable energy deployment. While FITs may be perceived by consumers as an extra cost, renewable energies cause a noticeable price reduction in wholesale electricity markets. We analyse both effects for the case of the Spanish electricity market during 2010. In particular, we examine the level of FITs that makes savings and extra costs to be similar on an hourly basis. Results are obtained for a wide range of renewable generation scenarios. It is found that FITs with null extra costs for consumers are in the range of 50–80 €/MWh. Some of the side-effects of a high penetration of renewable energy in the market are analysed in detail and discussed.