Influence of energy concentration and feed form of the diet on growth performance and digestive traits of brown egg-laying pullets from 1 to 120 days of age

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Heterologous Expression of a Plant Small Heat-Shock Protein Enhances Escherichia Coli Viability under Heat And Cold Stress Ref.: 1

A small heat-shock protein (sHSP) that shows molecular chaperone activity in vitro was recently purified from mature chestnut (Castanea sativa) cotyledons. This protein, renamed here as CsHSP17.5, belongs to cytosolic class I, as revealed by cDNA sequencing and immunoelectron microscopy. Recombinant CsHSP17.5 was overexpressed in Escherichia coli to study its possible function under stress conditions. Upon transfer from 37°C to 50°C, a temperature known to cause cell autolysis, those cells that accumulated CsHSP17.5 showed improved viability compared with control cultures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of cell lysates suggested that such a protective effect in vivo is due to the ability of recombinant sHSP to maintain soluble cytosolic proteins in their native conformation, with little substrate specificity. To test the recent hypothesis that sHSPs may be involved in protection against cold stress, we also studied the viability of recombinant cells at 4°C. Unlike the major heat-induced chaperone, GroEL/ES, the chestnut sHSP significantly enhanced cell survivability at this temperature. CsHSP17.5 thus represents an example of a HSP capable of protecting cells against both thermal extremes. Consistent with these findings, high-level induction of homologous transcripts was observed in vegetative tissues of chestnut plantlets exposed to either type of thermal stress but not salt stress

Dynamics interaction between rails and structure in a composite bridge of 120 m length

Dynamics interaction between rails and structure in a composite bridge of 120 m length

Same Virtus 120, versatilidad y economía sin complicaciones

El pasado día 17 de junio de 2014 tuvimos la oportunidad de realizar ensayos en campo a un tractor Same Virtus 120, en las instalaciones que la empresa posee junto a Toledo. La prueba de laboreo se realizó con un cultivador Kverneland CLC Evo con nueve brazos amortiguados con ballestas, rejas rectas y 2,4 m de anchura.También realizamos una prueba de transporte de un remolque con cebada, y evaluamos la maniobrabilidad y frenada del tractor.