Nitrogen uptake dynamics, yield and quality as influenced by nitrogen fertilization in "Piel de sapo" melon.

The need to reduce nitrogen (N) fertilizer pollution strengthens the importance of improving the utilization efficiency of applied N to crops. This requires knowledge of crop N uptake characteristics and how fertilization management affects it. A three-year field experiment was conducted from May to September in central Spain to investigate the influence of different N rates, which ranged from 11 to 393 kg ha-1, applied through drip irrigation, on the dynamics of N uptake, nitrogen use efficiency (NUE), fruit yield and quality of a ?Piel de sapo? melon crop (Cucumis melo L. cv. Sancho). Both N concentration and N content increased in different plant parts with the N rate. Leaves had the highest N concentration, which declined by 40-50% from 34-41 days after transplanting (DAT), while the highest N uptake rate was observed from 30-35 to 70-80 DAT, coinciding with fruit development. In each year, NUE declined with increasing N rate. With N fertilizer applications close to the optimum N rate of 90-100 kg ha-1, the fruits removed approximately 60 kg N ha-1, and the amount of N in the crop residue was about 80 kg N ha-1; this serves to replenish the organic nutrient pool in the soil and may be used by subsequent crops following mineralization.

Influence of fruit turgidity and firmness on apple bruise susceptibility.

Bruise damage is a major cause of quality loss for apples. It would be very useful to establish a method of characterizing bruise susceptibility in order to improve fruit handling, sometimes Magness-Taylor firmness is used as an indirect guide to handling requirements. The objective of the present work was to achieve a better bruise susceptibility prediction.

Sensing and modelling mechanical properties of fruits for quality.

Nondestructive techniques are extensively researched for the measurement of physical properties of fruits related to quality. Optical properties can be applied mainly in the detection of those quality features which are related to the chemical composition of the fruit, color (in the VIS region) or chemical constituents (sugar, in the MR region) being the most important. The most relevant mechanical property of fruits is consistency, generally called firmness, and to date only techniques which are able to measure the mechanical properties of the fruit bulk tissue are used for its prediction. Fruits can be modelled as elastic bodies, or at least as partially elastic. Therefore, the measurement of some elastic constants of the fruit can be used for the evaluation of its firmness. The differences in the response to loading are relevant in studying a) fruit firmness and b) bruising susceptibility. Both have been modelled for selected fruit species and varieties.

Quality assurance systems in the production and marketing of fresh peaches.

Due to the fast rate of peach post-harvest ripening, damage due to mechanical handling, externally appreciated as bruises and soft areas, is a real problem that leads to an early harvesting and poor quality of the fruits, as perceived by the consumers. More and more, the European consumer asks for good taste and freshness of fruits and vegetables, and these quality factors are not included in standards, nor in most of the producers' practices. Fruit processing and marketing centres (co-operatives) are increasingly interested in adopting quality controls in their processes. ISO 9000 procedures are being applied in some food areas, primarily milk and meat processors, but no generalised procedures have been developed until the present time to be applied to fresh product processes. All different peach and nectarine varieties that are harvested and handled in Murcia cooperatives and sold in a large supermarket in Madrid were analysed during the whole 1997 season (early May to late August). A total number of 78 samples of 25 fruits (co-operative) or 10 fruits (market), were tested in the laboratory for mechanical, optical, chemical and tasting quality. The variability and relationships between all these quality parameters are presented and discussed, and sampling unit sizes which would be advisable for quality control are calculated.

Testing equipment for the improvement of mechanical devices to minimize damage to fruit in commercial packing lines.

Damages -reduced in fruit packing lines is a major cause of grace reduction and quality loos in fresh marks: fruit. Fruit must be treated gently during in sir handling to improve their qualityin order to get a good price in a competitive market. The correct post-hardvest handling in fruit packing lines is a prerequisite to cut down the heavy post-harvest losses. Fruit packing lines must be evaluated, studying their design, the impacts applied to the fruits, the characteristics of the materials, etc. This study establishes the possibility of carrying out modifications and tests in a packing line during a long period of time. For this purpose, an experimental fruit packing line has been designed and located in the Agricultural Engineering Department of the Polythecnic University of Madrid with the aim of improving mechanical devices and fruit handling conditions to minimize damage to fruit. The experimental line consists of several transporting belts, one rollers transporter, one sizer, one elevator, one singularizer, and three trays to receive the calibrated fruit. The line has a length of 6.15 m and a width cf 1.9 m. Movement of the different components is regulated by electric motors with variable velocity electronically controlled. The height of the transfer points is variable and can be easily modified. The experimental line has been calibrated using two instrumented spheres IS 100 (8.8 cm Ø and6.2cm Ø). Average acceleration values obtained in all the transfers of the experimental line lay under 80 g's, although there is big variation for some of them being some values above 100 g's.

Determination of firmness in a fruit packing line using a non destructive impact sensor.

The increasing of quality fruit demanded by the consumers is originating an advance in the development and application of sensors capable of measuring parameters of quality (sugar, acids, firmness, etc) on a non destructive way. Some of these sensors are already operative for their use in laboratory and even in lines. The Physical Properties laboratory of the Polytechnic University of Madrid, is developing different sensors for their implementation in lines. One of them is a non destructive impact sensor to measure fruit firmness.

On-line MRI sequences for the evaluation of apple internal quality.

On-line dynamic MRI, which is oriented to industrial grading lines, requires high-speed sequences with motion correction artefacts. In this study two different types of motion correction sequences have been used and have been implemented in real-time (FLASH and UFLARE). They are based on T2* and T2 respectively and their selection depends on the expected contrast effect of the disorder: while watercore enhances bright areas due to higher fluid mobility, internal breakdown potentiates low signal due to texture degradation. For watercore study, five different apple cultivars were used (Normanda-18-, Fuji-35-, Helada-36-, Verde Doncella-54-, Esperiega-75-) along two seasons (2011 and 2012). In total 218 fruits were measured under both, static conditions (20 slices per fruit) and under dynamic conditions (3 repetitions without slice selection). For internal breakdown, Braeburn cultivar has been studied (in total 106 fruits) under both static (20 slices per fruit) and dynamic conditions (3 replicates with slice selection). Metrological aspects such as repeatability of dynamic images and subsequent histogram feature stability become of major interest for further industrial application. Segregation ability among varying degrees of disorder is also analyzed.

1H HR MAS NMR metabolomic and non-destructive 2D NMR relaxometry to assess internal quality in apples.

NMR can be considered a multi-scale multidimensional technology in the sense that it provides both spatial insight at macroscopic (MRI) or microscopic level (relaxometry), together with chemical characterization (HR-MAS). In this study 296 apples (from 4 cultivars) were MRI screened (20 slices per fruit) among which 7 fruits were used for metabolomic study by 1H HR MAS in order to assess various chemical shifts: malic acid, sucrose, glucose, fructose and ethanol. On the first season, tissue samples were taken from the sound and affected apples (near the core, centre and outer part of the mesocarp) belonging to sound and affected locations, while on the second season, tissue samples were focused on the comparison between sound and affected tissue. Beside, MRI and 2D non-destructive relaxometry (on whole fruits, and localized tissue) where performed on 72 and 12 apples respectively in order to compare features at macroscopic (tissue) and microscopic (subcellular) level. HR MAS shows higher content of ?-glucose, ?-glucose, malic acid and aromatic compounds in watercore affected tissues from both seasons, while sound tissue reflects higher sucrose. Microscopic (subcellular) degradation of tissue varies according to disorder development and is in good accordance with macroscopic characterization with MRI.

Jujube fruit water relations during fruit maturation stage under different water deficit conditions

The fruit maturation stage is considered the optimal phenological stage for implementing water deficitin jujube (Zizyphus jujuba Mill.), since a low, moderate or severe water deficit at this time has no effect onyield, fruit volume or eating quality. However, no information exists at fruit water relations level on themechanisms developed by Z. jujuba to confront drought. The purpose of the present study was to increaseour understanding of the relationship between leaf and fruit water relations of jujube plants under dif-ferent irrigation conditions during fruit maturation, paying special attention to analysing whether fruitsize depends on fruit turgor. For this, adult jujube trees (cv. Grande de Albatera) were subjected to fiveirrigation treatments. Control plants (T0) were irrigated daily above their crop water requirements inorder to attain non-limiting soil water conditions in 2012 and 2013. T1 plants were subjected to deficitirrigation throughout the 2012 season, according to the criteria frequently used by the growers in thearea. T2 (2012), T3 and T4 (2013) were irrigated as T0 except during fruit maturation, in which irrigationwas withheld for 32, 17 and 24 days, respectively. The results indicated that the jujube fruit maturationperiod was clearly sensitive to water deficit. During most of this stage water could enter the fruits viathe phloem rather than via the xylem. From the beginning of water withholding to when maximumwater stress levels were achieved, fruit and leaf turgor were maintained in plants under water deficit.However, a direct relation between turgor and fruit size was not found in jujube fruits, which could bedue to an enhancement of a cell elasticity mechanism (elastic adjustment) which maintains fruit turgorby reducing fruit cells size or to the fact that jujube fruit growth depends on the fruit growth-effectiveturgor rather than just turgor pressure.