Isolamento e caracterização de uma proteína tóxica produzida por um isolado açoriano de Bacillus weihenstephanensis

Dissertação de Mestrado, Biotecnologia em Controlo Biológico, 27 de Junho de 2013, Universidade dos Açores.

Building the «true evolutionism»: Darwin's impact on Henri Bergson's thought

The paper focuses on the importance of Darwin’s work for the shaping of Henri Bergson’s philosophy, bearing on mind that the two authors first intercepted symbolically in 1859, when On the Origin of Species was published and Bergson was born. Bergson studied the biological sciences of his time, whose results were integrated in a metaphysical thought. He belonged to spiritualistic positivism, a philosophy that goes from the positive data of sciences and finds the ultimate explanation of reality in a spiritual principle. He was interested in the positive evolution of the natural world and in the works of naturalists such as Lamarck, De Vries or Eimer. Darwin was among these authors, being responsible for a vision of evolution that went from the scientific level to other domains. Bergson defends the “insufficiency of pure Darwinism” by pointing out the necessity to compensate scientific evolution with an internal metaphysical reading of the real, which he considered to be “true evolutionism”. This criticism is the most visible aspect of the relations between both works. However, an attentive look verifies that Darwin’s influence overcomes the divergence of positions concerning the extent of “evolution”. The French philosopher knew not only the 1859’s bestseller, but also studies by Darwin about ethology, entomology and botany, which contributed to the fact that the naturalist’s impact gained fundamental importance in Bergson’s philosophical perspective.

Pittosporum Undulatum and Hedychium Gardnerianum: nutritive value and secondary metabolites on cattle reproductive performances

Hedychium gardnerianum (HG) and Pittospporum undulatum (PU) are invasive plants all over the world, being in the Azores supplied to cattle on periods of shortage food. As these plants produce secondary metabolites, including a diverse range of phytochemicals compounds, the aim of the presente study is to identify how these metabolites can be related to animal’s reproductive performances. For such purpose, plants were harvested on winter, compounds extracted by method of decoction and analysed by combination of liquid chromatography and mass spectrometry as well as highperformance liquid chromatography. For nutritive evaluations, Van Soest and Weende methodologies were used. In HG quercetin-3, 4'-di-O-betaglucopyranoside, myricetin rhamnoside, quercetin rhamnoside, and gibberellin A1 and A8 were identified, while for PU were found cafeic acid derivatives, including dicaffeoylquinic acid and caffeoylquinic acid. In nutritional terms, these plants can be considered as poor, presenting percentages of dry matter (DM%) of 16.34% and 40.39%, respectively for HG and PU. Values for ash 10.4%, crude protein (CP) 7.75%, neutral detergent fiber (NDF) 64.5, acid detergent fiber (ADF) 34.69%, acid detergent lignin (ADL) 3.47% and ether extract (EE) 2.03% were found for HG. For PU values were ash 6.64%, CP 6.11%, NDF 43.84%, acid ADF 35.57%, ADL 3.56% and EE 2.71%. This study clearly indicated that, besides their low nutritive values, these plants can be used to feed ruminants, especially when pasture lacks. Nevertheless, as some compounds, namely the caffeoylquinic and dicaffeoylquinic acids, are known to be associated to physiological reproductive mechanisms, one could speculate that these compounds can be directly or indirectly associated to reproductive performances in bovine fed with these plants.