1 resultado para Multidimensional deprivation
em Instituto Gulbenkian de Ciência
Filtro por publicador
- JISC Information Environment Repository (1)
- Repository Napier (1)
- Aberdeen University (2)
- Academic Research Repository at Institute of Developing Economies (2)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (3)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (8)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (3)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (2)
- Archive of European Integration (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (16)
- Argos - Repositorio Institucional de la Secretaría de Investigación y Postgrado de la Facultad de Humanidades y Ciencias Sociales de la Universidad Nacional de Misiones (2)
- Aston University Research Archive (15)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (2)
- Biblioteca Digital da Câmara dos Deputados (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (5)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (3)
- Biblioteca Digital de la Universidad Católica Argentina (5)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (5)
- Bibloteca do Senado Federal do Brasil (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (46)
- Boston University Digital Common (1)
- Brock University, Canada (2)
- Bucknell University Digital Commons - Pensilvania - USA (4)
- Bulgarian Digital Mathematics Library at IMI-BAS (5)
- CaltechTHESIS (4)
- Cambridge University Engineering Department Publications Database (17)
- CentAUR: Central Archive University of Reading - UK (14)
- Center for Jewish History Digital Collections (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (13)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (11)
- CORA - Cork Open Research Archive - University College Cork - Ireland (1)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (2)
- Deakin Research Online - Australia (78)
- Digital Commons - Michigan Tech (3)
- Digital Commons at Florida International University (3)
- Digital Peer Publishing (1)
- DigitalCommons@The Texas Medical Center (2)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (1)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (9)
- eScholarship Repository - University of California (1)
- Helda - Digital Repository of University of Helsinki (37)
- Indian Institute of Science - Bangalore - Índia (74)
- Instituto Gulbenkian de Ciência (1)
- Instituto Politécnico de Leiria (1)
- Instituto Politécnico do Porto, Portugal (11)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (3)
- Memoria Académica - FaHCE, UNLP - Argentina (19)
- Ministerio de Cultura, Spain (26)
- National Center for Biotechnology Information - NCBI (16)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (113)
- Queensland University of Technology - ePrints Archive (200)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- RDBU - Repositório Digital da Biblioteca da Unisinos (2)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (6)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional da Universidade Federal do Rio Grande - FURG (2)
- Repositorio Institucional de la Universidad de El Salvador (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (50)
- Research Open Access Repository of the University of East London. (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (2)
- Scielo España (3)
- Universidad de Alicante (4)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (8)
- Universidade de Lisboa - Repositório Aberto (3)
- Universidade Federal do Pará (2)
- Universidade Federal do Rio Grande do Norte (UFRN) (7)
- Universidade Técnica de Lisboa (1)
- Universitat de Girona, Spain (3)
- Université de Montréal, Canada (7)
- University of Connecticut - USA (1)
- University of Michigan (2)
- University of Queensland eSpace - Australia (15)
- University of Southampton, United Kingdom (2)
- University of Washington (1)
- WestminsterResearch - UK (2)
Resumo:
Since years, research on SnRK1, the major cellular energy sensor in plants, has tried to define its role in energy signalling. However, these attempts were notoriously hampered by the lethality of a complete knockout of SnRK1. Therefore, we generated an inducible amiRNA::SnRK1α2 in a snrk1α1 knock out background (snrk1α1/α2) to abolish SnRK1 activity to understand major systemic functions of SnRK1 signalling under energy deprivation triggered by extended night treatment. We analysed the in vivo phosphoproteome, proteome and metabolome and found that activation of SnRK1 is essential for repression of high energy demanding cell processes such as protein synthesis. The most abundant effect was the constitutively high phosphorylation of ribosomal protein S6 (RPS6) in the snrk1α1/α2 mutant. RPS6 is a major target of TOR signalling and its phosphorylation correlates with translation. Further evidence for an antagonistic SnRK1 and TOR crosstalk comparable to the animal system was demonstrated by the in vivo interaction of SnRK1α1 and RAPTOR1B in the cytosol and by phosphorylation of RAPTOR1B by SnRK1α1 in kinase assays. Moreover, changed levels of phosphorylation states of several chloroplastic proteins in the snrk1α1/α2 mutant indicated an unexpected link to regulation of photosynthesis, the main energy source in plants.