Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line


Autoria(s): Dehler, Carola E; Boudinot, Pierre; Martin, Samuel A M; Collet, Bertrand
Contribuinte(s)

University of Aberdeen, School of Biological Sciences, Biological Sciences

University of Aberdeen, MASTS (Marine Alliance for Science and Technology for Scotland)

University of Aberdeen, Scottish Fish Immunology Research Centre

Data(s)

09/09/2016

09/09/2016

01/08/2016

Resumo

This project was carried out during CD’s BBSRC Eastbio PIPS placement at Marine Scotland. The authors are grateful to Dr Milena Monte (University of Aberdeen) for help with the FACS analysis. The authors wish to thank Dr Filippo Del Bene (Neuronal Circuit Development, Institut Curie) and Dr Wenbiao Chen (School of Medicine, Vanderbilt University) for the Addgene plasmids, #61051 and #47929, respectively, and Prof. Nancy C. Reich Marshall (Department of Molecular Genetics and Microbiology, Stony Brooks University) for the plasmid pmEGFP-N1.

Peer reviewed

Publisher PDF

Formato

4

Identificador

Dehler , C E , Boudinot , P , Martin , S A M & Collet , B 2016 , ' Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line ' Marine Biotechnology , vol 18 , no. 4 , pp. 449-452 . , 10.1007/s10126-016-9708-6

1436-2228

PURE: 67084583

PURE UUID: 0128d7b6-9121-499c-86e2-57088771042e

http://hdl.handle.net/2164/7464

http://dx.doi.org/10.1007/s10126-016-9708-6

Idioma(s)

eng

Relação

Marine Biotechnology

Direitos

https://creativecommons.org/licenses/by/4.0/

Palavras-Chave #CRISPR/Cas9 #CHSE-214 #EGFP #FACS #genome editing #nCas9n #QH301 Biology #Biotechnology and Biological Sciences Research Council (BBSRC) #QH301
Tipo

Journal article