Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells.


Autoria(s): Zheng, Dinghai; Ezzeddine, Nader; Chen, Chyi-Ying A; Zhu, Wenmiao; He, Xiangwei; Shyu, Ann-Bin
Data(s)

14/07/2008

Resumo

Deadenylation is the major step triggering mammalian mRNA decay. One consequence of deadenylation is the formation of nontranslatable messenger RNA (mRNA) protein complexes (messenger ribonucleoproteins [mRNPs]). Nontranslatable mRNPs may accumulate in P-bodies, which contain factors involved in translation repression, decapping, and 5'-to-3' degradation. We demonstrate that deadenylation is required for mammalian P-body formation and mRNA decay. We identify Pan2, Pan3, and Caf1 deadenylases as new P-body components and show that Pan3 helps recruit Pan2, Ccr4, and Caf1 to P-bodies. Pan3 knockdown causes a reduction of P-bodies and has differential effects on mRNA decay. Knocking down Caf1 or overexpressing a Caf1 catalytically inactive mutant impairs deadenylation and mRNA decay. P-bodies are not detected when deadenylation is blocked and are restored when the blockage is released. When deadenylation is impaired, P-body formation is not restorable, even when mRNAs exit the translating pool. These results support a dynamic interplay among deadenylation, mRNP remodeling, and P-body formation in selective decay of mammalian mRNA.

Identificador

http://digitalcommons.library.tmc.edu/uthmed_docs/331

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2584600/?tool=pmcentrez

Publicador

DigitalCommons@The Texas Medical Center

Fonte

UT Medical School Journal Articles

Palavras-Chave #Animals #Cytoplasmic Structures #Humans #Mice #Models #Biological #Multiprotein Complexes #NIH 3T3 Cells #Poly A #Polyadenylation #Protein Binding #Protein Transport #Proteins #Puromycin #RNA Stability #RNA #Messenger #Receptors #CCR4 #Models, Biological #RNA, Messenger #Receptors, CCR4 #Medicine and Health Sciences
Tipo

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