Amperometric urea biosensors based on the entrapment of urease in polypyrrole films
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
01/11/2013
01/11/2013
2012
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| Resumo |
Urease (Urs) was immobilized in electrochemically prepared polypyrrole (PPy) and the resulting films were characterized by cyclic voltammetry, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and ultraviolet visible spectroscopy (UV-VIS). The enzymatic activity of Urs entrapped in the PPy matrix was confirmed by the catalytic conversion of urea into carbon dioxide and ammonia, when urea was detected amperometrically at different concentrations in standard samples and commercial fertilizers. The PPy/Urs biosensors exhibited selectivity, a relatively high efficiency at urea concentrations below 3.0 mmol L-1, and a sensitivity to urea of 2.41 mu A cm(-2) mmol(-1) L (C) 2011 Elsevier Ltd. All rights reserved. FAPESP FAPESP CAPES CAPES CNPq CNPq |
| Identificador |
REACTIVE & FUNCTIONAL POLYMERS, AMSTERDAM, v. 72, n. 2, supl. 1, Part 1, pp. 148-152, FEB, 2012 1381-5148 http://www.producao.usp.br/handle/BDPI/37151 10.1016/j.reactfunctpolym.2011.12.002 |
| Idioma(s) |
eng |
| Publicador |
ELSEVIER SCIENCE BV AMSTERDAM |
| Relação |
REACTIVE & FUNCTIONAL POLYMERS |
| Direitos |
closedAccess Copyright ELSEVIER SCIENCE BV |
| Palavras-Chave | #BIOSENSORS #CONDUCTING POLYMERS #POLYPYRROLE #UREA #UREASE #GLASS-PH-ELECTRODE #BLOOD-SAMPLES #AMMONIA #CONDUCTIVITY #COMPOSITES #MORPHOLOGY #REDUCTION #OXIDATION #ENZYME #SENSOR #CHEMISTRY, APPLIED #ENGINEERING, CHEMICAL #POLYMER SCIENCE |
| Tipo |
article original article publishedVersion |
