Lepstospira interrogans shotgun phage display identified LigB as a heparin-binding protein
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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| Data(s) |
30/10/2013
30/10/2013
2012
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| Resumo |
LigB is an adhesin from pathogenic Leptospira that is able to bind to extracellular matrix and is considered a virulence factor. A shotgun phage display genomic library was constructed and used for panning against Heparan Sulfate Proteoglycan (HSPG). A phage clone encoding part of LigB protein was selected in panning experiments and showed specific binding to heparin. To validate the selected clone, fragments of LigB were produced as recombinant proteins and showed affinity to heparin and to mammalian cells. Heparin was also able to reduce the binding of rLB-Ct to mammalian cells. Our data suggests that the glycosaminoglycan moiety of the HSPG is responsible for its binding and could mediate the attachment of the recombinant protein rLB-Ct. Thus, heparin may act as a receptor for Leptospira to colonize and to invade the host tissue. (C) 2012 Elsevier Inc. All rights reserved. FAPESP FAPESP CNPq CNPq Fundacao Butantan Fundacao Butantan |
| Identificador |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, SAN DIEGO, v. 427, n. 4, supl. 1, Part 3, pp. 774-779, 37561, 2012 0006-291X http://www.producao.usp.br/handle/BDPI/36883 10.1016/j.bbrc.2012.09.137 |
| Idioma(s) |
eng |
| Publicador |
ACADEMIC PRESS INC ELSEVIER SCIENCE SAN DIEGO |
| Relação |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
| Direitos |
closedAccess Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE |
| Palavras-Chave | #LEPTOSPIRA INTERROGANS #HEPARIN #PHAGE DISPLAY #LIGB #IMMUNOGLOBULIN-LIKE PROTEIN #HOST-PATHOGEN INTERACTIONS #LYME-DISEASE SPIROCHETE #LEPTOSPIRA-INTERROGANS #BORRELIA-BURGDORFERI #FIBRONECTIN-BINDING #SURFACE PROTEIN #C-TERMINUS #LAMININ #PROTEOGLYCANS #BIOCHEMISTRY & MOLECULAR BIOLOGY #BIOPHYSICS |
| Tipo |
article original article publishedVersion |
