Evaluation of genotoxicity and oxidative damage in painters exposed to low levels of toluene
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
24/10/2013
24/10/2013
2012
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Resumo |
Toluene is an organic solvent used in numerous processes and products, including industrial paints. Toluene neurotoxicity and reproductive toxicity are well recognized: however, its genotoxicity is still under discussion, and toluene is not classified as a carcinogenic solvent. Using the comet assay and the micronucleus test for detection of possible genotoxic effects of toluene, we monitored industrial painters from Rio Grande do Sul, Brazil. The putative involvement of oxidative stress in genetic damage and the influences of age, smoking, alcohol consumption, and exposure time were also assessed. Although all biomarkers of toluene exposure were below the biological exposure limits, painters presented significantly higher DNA damage (comet assay) than the control group; however, in the micronucleus assay, no significant difference was observed. Painters also showed alterations in hepatic enzymes and albumin levels, as well as oxidative damage, suggesting the involvement of oxidative stress. According to multiple linear regression analysis, blood toluene levels may account for the increased DNA damage in painters. In summary, this study showed that low levels of toluene exposure can cause genetic damage, and this is related to oxidative stress, age, and time of exposure. (C) 2012 Elsevier B.V. All rights reserved. CNPq (S.C. Garcia) [477740/2007-3, 479613/2009-5] CNPq (S.C. Garcia) CNPq/MCT (Paulo Saldiva) CNPq/MCT (Paulo Saldiva) CNPq [308020/2009-0] CNPq |
Identificador |
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, AMSTERDAM, v. 746, n. 1, supl. 4, Part 1, pp. 42-48, 38169, 2012 1383-5718 http://www.producao.usp.br/handle/BDPI/35985 10.1016/j.mrgentox.2012.02.007 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE BV AMSTERDAM |
Relação |
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS |
Direitos |
closedAccess Copyright ELSEVIER SCIENCE BV |
Palavras-Chave | #TOLUENE #GENOTOXICITY #COMET ASSAY #MICRONUCLEUS ASSAY #OXIDATIVE STRESS #PERFORMANCE LIQUID-CHROMATOGRAPHY #BRAZILIAN FOOTWEAR-WORKERS #ISCHEMIA-MODIFIED ALBUMIN #GENETIC-DAMAGE #DNA-DAMAGE #COMET ASSAY #OCCUPATIONAL-EXPOSURE #IN-VITRO #BIOMARKERS #STRESS #BIOTECHNOLOGY & APPLIED MICROBIOLOGY #GENETICS & HEREDITY #TOXICOLOGY |
Tipo |
article original article publishedVersion |