Evaluation of genotoxicity and oxidative damage in painters exposed to low levels of toluene


Autoria(s): Moro, Angela M.; Brucker, Natalia; Charao, Mariele; Bulcao, Rachel; Freitas, Fernando; Baierle, Marilia; Nascimento, Sabrina; Valentini, Juliana; Cassini, Carina; Salvador, Mirian; Linden, Rafael; Thiesen, Flavia; Buffon, Andreia; Moresco, Rafael; Garcia, Solange C.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

24/10/2013

24/10/2013

2012

Resumo

Toluene is an organic solvent used in numerous processes and products, including industrial paints. Toluene neurotoxicity and reproductive toxicity are well recognized: however, its genotoxicity is still under discussion, and toluene is not classified as a carcinogenic solvent. Using the comet assay and the micronucleus test for detection of possible genotoxic effects of toluene, we monitored industrial painters from Rio Grande do Sul, Brazil. The putative involvement of oxidative stress in genetic damage and the influences of age, smoking, alcohol consumption, and exposure time were also assessed. Although all biomarkers of toluene exposure were below the biological exposure limits, painters presented significantly higher DNA damage (comet assay) than the control group; however, in the micronucleus assay, no significant difference was observed. Painters also showed alterations in hepatic enzymes and albumin levels, as well as oxidative damage, suggesting the involvement of oxidative stress. According to multiple linear regression analysis, blood toluene levels may account for the increased DNA damage in painters. In summary, this study showed that low levels of toluene exposure can cause genetic damage, and this is related to oxidative stress, age, and time of exposure. (C) 2012 Elsevier B.V. All rights reserved.

CNPq (S.C. Garcia) [477740/2007-3, 479613/2009-5]

CNPq (S.C. Garcia)

CNPq/MCT (Paulo Saldiva)

CNPq/MCT (Paulo Saldiva)

CNPq [308020/2009-0]

CNPq

Identificador

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, AMSTERDAM, v. 746, n. 1, supl. 4, Part 1, pp. 42-48, 38169, 2012

1383-5718

http://www.producao.usp.br/handle/BDPI/35985

10.1016/j.mrgentox.2012.02.007

http://dx.doi.org/10.1016/j.mrgentox.2012.02.007

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

AMSTERDAM

Relação

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

Direitos

closedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #TOLUENE #GENOTOXICITY #COMET ASSAY #MICRONUCLEUS ASSAY #OXIDATIVE STRESS #PERFORMANCE LIQUID-CHROMATOGRAPHY #BRAZILIAN FOOTWEAR-WORKERS #ISCHEMIA-MODIFIED ALBUMIN #GENETIC-DAMAGE #DNA-DAMAGE #COMET ASSAY #OCCUPATIONAL-EXPOSURE #IN-VITRO #BIOMARKERS #STRESS #BIOTECHNOLOGY & APPLIED MICROBIOLOGY #GENETICS & HEREDITY #TOXICOLOGY
Tipo

article

original article

publishedVersion