Digestive peptidases and proteinases in the midgut gland of the pink shrimp Farfantepenaeus paulensis (Crustacea, Decapoda, Penaeidae)


Autoria(s): BUARQUE, Diego Souza; CASTRO, Patricia Fernandes; SANTOS, Fabio Marcel Silva; LEMOS, Daniel; CARVALHO JUNIOR, Luiz Bezerra; BEZERRA, Ranilson Souza
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

Proteases from the midgut gland of the Farfantepenaeus paulensis juveniles were assessed. Enzyme activity was determined using protease substrates and inhibitors. The effect of pH, temperature and calcium on proteolytic activity was assayed. Caseinolytic activity was analysed in substrate-sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Trypsin, chymotrypsin and leucine aminopeptidase activity was detected. Proteolytic activity was strongly inhibited by the specific trypsin inhibitors. Tosyl-phenylalanine chloromethyl ketone inhibited 59.3% of chymotrypsin activity. The greatest trypsin-like activity occurred at pH 8.0 and 45 degrees C. Chymotrypsin-like activity reached maximal values at alkaline pH (7.2-9.0) and 55 degrees C. CaCl(2) did not increase trypsin-like activity, but rather inhibited it at concentrations of 30 (20%), 50 (30%) and 100 mM (50%). The substrate-SDS-PAGE zymogram revealed eight proteinase bands. Two possibly thermal-resistant (85 degrees C, 30 min) chymotrypsin isoforms were found, which were inhibited by phenyl-methyl-sulphonyl-fluoride. Aminopeptidase activity of enzyme extracts (Arg, Leu, Lys, Phe and Val) and the recommended concentrations of these essential amino acids in penaeid shrimp diets were positively correlated (P < 0.05). Beause protein digestion involves the combined action of different enzymes, adequate knowledge of shrimp digestion and enzyme characteristics is required for the assessment of the digestive potential of different feed sources and development of in vitro digestibility protocols.

FINEP/RECARCINE

Financiadora de Estudos e Projetos (FINEP)

SEAP/PR

SEAP/PR

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

CNPq

FACEPE

FACEPE

PETROBRAS

PETROBRAS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP[05/50578-2]

FAPESP[07/07051-9]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

CNPq/SEAP[504031/2003-1]

CNPq/SEAP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

CNPq fellowship[308444/2006-0]

National Research System (Brazil)

National Research System (Brazil)

Identificador

AQUACULTURE RESEARCH, v.40, n.7, p.861-870, 2009

1355-557X

http://producao.usp.br/handle/BDPI/31977

10.1111/j.1365-2109.2009.02183.x

http://dx.doi.org/10.1111/j.1365-2109.2009.02183.x

Idioma(s)

eng

Publicador

WILEY-BLACKWELL PUBLISHING, INC

Relação

Aquaculture Research

Direitos

restrictedAccess

Copyright WILEY-BLACKWELL PUBLISHING, INC

Palavras-Chave #trypsin #chymotrypsin #aminopeptidase #protein digestion #substrate-SDS-PAGE #Farfantepenaeus subtilis #PENAEUS-VANNAMEI #ONTOGENIC VARIATION #MESSENGER-RNA #ENZYMES #TRYPSIN #PURIFICATION #FEED #DIGESTIBILITY #INHIBITORS #PROTEASES #Fisheries
Tipo

article

original article

publishedVersion