An impedimetric biosensor to test neat serum for dengue diagnosis
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
21/10/2015
21/10/2015
05/07/2015
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Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 2012-22820-7 NS1, a non-structural dengue protein, has been used as a biomarker for the detection of viremia in dengue diagnosis. In this work, an impedimetric label-free immunosensor based on an anti-NS1 modified gold electrode was developed. To immobilize anti-NS1, a mixed self-assembled monolayer consisting of 11-mercaptoundecanoic acid (for covalent anti-NS1 attachment) and 6-mercaptohexanol (as a spacer) was prepared. The impedance spectra were recorded in the presence of a redox probe ( [Fe(CN)(6)](3-/4-)) to monitor changes in charge-transfer resistance associated with target binding both in PBS and neat serum. A calibration curve was constructed in order to obtain analytical parameters. NS1 was diluted in PBS to final concentrations in the linear range of 0.01-2.00 mu g mL(-1) with a sensitivity of 14.1 percentage decade(-1) (R similar to 0.99) and a limit of detection (LOD) of 3 ng mL(-1). A linear range 0.01-1.00 mu g mL(-1) of NS1 diluted in neat serum was also obtained (R similar to 0.98) with a sensitivity of 10.4 percentage decade(-1) and a LOD of 30 ng mL(-1). The results open new insights into the potential use of this biosensor for point-of-care and bedside applications for dengue diagnosis. |
Formato |
150-154 |
Identificador |
http://www.sciencedirect.com/science/article/pii/S0925400515002427 Sensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 213, p. 150-154, 2015. 0925-4005 http://hdl.handle.net/11449/129089 http://dx.doi.org/10.1016/j.snb.2015.02.068 WOS:000352152500020 |
Idioma(s) |
eng |
Publicador |
Elsevier B.V. |
Relação |
Sensors And Actuators B-chemical |
Direitos |
closedAccess |
Palavras-Chave | #Dengue #NS1 protein #Electrochemical impedance spectroscopy (EIS) #Label-free biosensor #Blood serum |
Tipo |
info:eu-repo/semantics/article |